Updated on 2026/04/05

写真a

 
KOGA Daisuke
 
Organization
School of Medicine Medical Course Basic Medicine Anatomy[Microscopic Anatomy and Cell Biology]
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Degree

  • 博士(医学) ( 2007.3   新潟大学 )

  • 博士 (医学) ( 新潟大学 )

Research Interests

  • 細胞・組織

  • 細胞分裂

  • 立体構造

  • 解剖学

  • 細胞

  • オスミウム浸軟法

  • ゴルジ装置

  • 細胞小器官

  • 走査電子顕微鏡

  • 下垂体

  • 組織

  • 細胞微細形態学

  • 電子顕微鏡

  • SEM

Research Areas

  • Life Science / Anatomy

  • Life Science / Anatomy

  • Life Science / Morphology, anatomy

Research History

  • 東北大学 理学部 生物学科 非常勤講師

    2026

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  • 東北大学 理学部 生物学科 非常勤講師

    2023 - 2024

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  • 北海道大学 大学院医学院 非常勤講師

    2017

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  • Asahikawa Medical College   Associate Professor

    2015.5

  • Asahikawa Medical University   School of Medicine   Associate Professor

    2015.5

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  • Niigata University   Graduate School of Medical and Dental Sciences   Lecturer

    2013.7 - 2015.4

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  • Niigata University   Graduate School of Medical and Dental Sciences   Assistant Professor

    2007.10 - 2013.6

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  • 県立新潟女子短期大学 食物栄養専攻 非常勤講師

    2004.4 - 2008.9

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Professional Memberships

  • 下垂体研究会

    2012.4

  • 日本解剖学会

    2006.4

  • 日本顕微鏡学会

    2005.4

  • THE JAPANESE ASSOCIATION OF ANATOMISTS

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  • THE JAPANESE SOCIETY OF MICROSCOPY

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  • 下垂体研究会

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Committee Memberships

  • 日本解剖学会   ダイバーシティ委員会委員  

    2025.4   

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  • 日本顕微鏡学会   代議員  

    2025.4   

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  • 日本解剖学会   認定解剖組織技術者資格審査委員会委員  

    2025.4   

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  •   細胞小器官用語委員会委員  

    2024.12   

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  • 日本顕微鏡学会   代議員  

    2023.4 - 2025.3   

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  • 日本顕微鏡学会   和文誌「顕微鏡」編集委員  

    2019.4 - 2025.3   

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    Committee type：Academic society

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  • 日本解剖学会   「若手研究者の会」運営委員 教育研究キャリア班  

    2019.4 - 2021.3   

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  •   Biomedical Research 誌 編集委員  

    2019   

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  • 日本解剖学会   評議員  

    2018   

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    Committee type：Academic society

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  • 日本顕微鏡学会   北海道支部役員  

    2017   

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  • 日本顕微鏡学会   走査電子顕微鏡分科会幹事  

    2016.4 - 2023.3   

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  • 日本解剖学会   学術委員会委員  

    2016.4 - 2019.3   

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  • 日本顕微鏡学会   代議員  

    2015.4 - 2017.3   

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  • 日本顕微鏡学会   技術認定委員  

    2014   

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  • 日本顕微鏡学会   代議員  

    2013.4 - 2015.3   

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  • 下垂体研究会   評議員  

    2013   

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  • 日本顕微鏡学会   関東支部役員  

    2012.4 - 2014.3   

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    Committee type：Academic society

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Papers

  • Three-dimensional morphological analysis of ghrelin-producing cells in the rat fundic gland by combining serial section scanning electron microscopy and immunogold technique Reviewed

    Daisuke KOGA, Satoshi KUSUMI, Takahiro SATO

    Biomedical Research   47 ( 1 )   35 - 46   2026.1

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    Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Biomedical Research Press  

    DOI： 10.2220/biomedres.47.35

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  • A novel 3D immuno-electron microscopy and its application to the Golgi apparatus. Reviewed

    Daisuke Koga, Satoshi Kusumi, Hirokazu Yagi, Koichi Kato

    Biomedical research (Tokyo, Japan)   47 ( 1 )   25 - 34   2026

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Serial section scanning electron microscopy (SEM) is useful for revealing the three-dimensional (3D) architecture of organelles by acquiring backscattered electron images of ultrathin serial sections of resin-embedded tissues on solid substrates. However, comprehensive analyses of organelle function require a combination of ultrastructural and molecular localization data. In the present study, we developed a novel 3D immuno-electron microscopy (immuno-EM) approach that combines Tokuyasu cryosectioning with serial section SEM to elucidate the spatial distribution of organelle-associated proteins. Thick cryosections of tissues were immunolabeled with primary antibodies and FluoroNanogold-conjugated secondary antibodies, followed by gold enhancement, resin embedding, and serial sectioning and SEM. Serial tomographic images of organelles were aligned and segmented to generate 3D reconstructions. To demonstrate the effectiveness of the method, we visualized the localization of GM130, a representative cis-Golgi matrix protein, in a 3D model of the Golgi apparatus in rat pituitary gonadotropes. The 3D model revealed a spherical Golgi apparatus composed of five cisternae arranged in cis-trans order, with GM130 localized on the outer cisternae, consistent with previous findings. Our 3D immuno-EM technique enables the detailed 3D visualization of the Golgi apparatus and other organelles as well as analyses of the spatial distribution of target proteins in their 3D reconstructions.

    DOI： 10.2220/biomedres.47.25

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  • Three-Dimensional Ultrastructure of the Golgi Apparatus In Vivo: Scanning Electron Microscopy of Osmium-Macerated Mammalian Cells. Invited Reviewed International journal

    Daisuke Koga, Ryosuke Morinaga, Satoshi Kusumi

    Sub-cellular biochemistry   110   1 - 34   2026

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    The osmium maceration method remains the only technique that enables direct three-dimensional (3D) visualization of subcellular structures via scanning electron microscopy (SEM), and it is particularly effective for resolving spatially complex organelles such as the Golgi apparatus. Here, we describe the 3D ultrastructure of the Golgi apparatus in various cell types, including epididymal epithelial principal cells, intestinal goblet cells, pituitary gonadotropes, and spinal ganglion cells, processed using the osmium maceration method. The overall morphology of the Golgi apparatus varied between cell types, exhibiting a range of configurations, including cup-shaped and spherical forms. Within the Golgi stacks, the cis-most cisterna and the trans-Golgi network displayed especially distinct structural characteristics. The cis-most cisterna typically appeared as a flat sheet with numerous fenestrations, a feature consistently observed across all four cell types. In contrast, the 3D organization of the trans-Golgi network, comprising tubules and/or plate-like membranes frequently interconnected to form elaborate architectures, varied according to the cell type. The morphological diversity of the Golgi apparatus in three dimensions likely reflects functional and structural specializations among different cell types.

    DOI： 10.1007/978-3-032-06936-8_1

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  • 3D免疫電子顕微鏡法とゴルジ装置の形態解析への応用 Invited Reviewed

    甲賀大輔, 久住聡, 矢木宏和, 加藤晃一

    顕微鏡   2026

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  • Direct imaging of the three-dimensional ultrastructure of neuronal organelles. Invited Reviewed

    Daisuke Koga, Ryosuke Morinaga, Satoshi Kusumi

    Anatomical science international   2025.8

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    In the context of cell morphological analyses observing organelles embedded within the cell matrix is difficult. The osmium maceration method is a unique technique used to directly observe the three-dimensional structure of organelles through scanning electron microscopy, without requiring time-consuming and labor-intensive reconstruction. In this method, tissues are immersed in a diluted osmium solution for several days to remove cytosolic soluble proteins and filamentous structures, including microfilaments, intermediate filaments, and microtubules, from the freeze-cracked surfaces of cells, leaving the subcellular structures, Golgi apparatus, mitochondria, and smooth and rough endoplasmic reticulum intact. Specimen preparation involves several key steps, specifically pre-fixation with aldehyde fixatives, tissue excision, trimming, post-fixation with osmium tetroxide solution, dimethyl sulfoxide cracking (i.e., freeze-cracking), the thawing of cracked tissues, osmium maceration, osmium fixation, conductive staining (tannin-osmium method), dehydration, drying, mounting, metal coating, and scanning electron microscopy observations. Here, we present a step-by-step protocol based on the maceration method using neural cells as an example, ensuring reproducibility and consistent results for neurons and various other cell types. Moreover, the results presented indicate that the osmium maceration method is effective for elucidating the three-dimensional intracellular ultrastructure of neurons.

    DOI： 10.1007/s12565-025-00888-5

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  • Dysregulation of PI4P in the trans Golgi regions activates the mammalian Golgi stress response Reviewed

    Kanae Sasaki, Marika Toide, Takuya Adachi, Fumi Morishita, Yuto Watanabe, Hajime Tajima Sakurai, Sadao Wakabayashi, Satoshi Kusumi, Toshiyuki Yamaji, Kaori Sakurai, Daisuke Koga, Kentaro Hanada, Masafumi Yohda, Hiderou Yoshida

    Journal of Biological Chemistry   301 ( 1 )   108075 - 108075   2025.1

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.jbc.2024.108075

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  • 走査電子顕微鏡による下垂体前葉細胞オルガネラの 3D イメージングに関する研究

    日本下垂体研究会誌   12   1 - 7   2025

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  • Secretion of endoplasmic reticulum protein VAPB/ALS8 requires topological inversion Reviewed

    Kosuke Kamemura, Rio Kozono, Mizuki Tando, Misako Okumura, Daisuke Koga, Satoshi Kusumi, Kanako Tamai, Aoi Okumura, Sayaka Sekine, Daichi Kamiyama, Takahiro Chihara

    Nature Communications   15 ( 1 )   2024.10

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1038/s41467-024-53097-5

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    Other Link： https://www.nature.com/articles/s41467-024-53097-5

  • Three-dimensional analysis of the intracellular architecture by scanning electron microscopy Invited Reviewed

    Daisuke Koga, Satoshi Kusumi, Hirokazu Yagi, Koichi Kato

    Microscopy   73 ( 3 )   215 - 225   2024.6

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    Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    The two-dimensional observation of ultrathin sections from resin-embedded specimens provides an insufficient understanding of the three-dimensional (3D) morphological information of membranous organelles. The osmium maceration method, developed by Professor Tanaka’s group >40 years ago, is the only technique that allows direct observation of the 3D ultrastructure of membrane systems using scanning electron microscopy (SEM), without the need for any reconstruction process. With this method, the soluble cytoplasmic proteins are removed from the freeze-cracked surface of cells while preserving the integrity of membranous organelles, achieved by immersing tissues in a diluted osmium solution for several days. By employing the maceration method, researchers using SEM have revealed the 3D ultrastructure of organelles such as the Golgi apparatus, mitochondria and endoplasmic reticulum in various cell types. Recently, we have developed new SEM techniques based on the maceration method to explore further possibilities of this method. These include: (i) a rapid osmium maceration method that reduces the reaction duration of the procedure, (ii) a combination method that combines agarose embedding with osmium maceration to elucidate the 3D ultrastructure of organelles in free and cultured cells and (iii) a correlative immunofluorescence and SEM technique that combines cryosectioning with the osmium maceration method, enabling the correlation of the immunocytochemical localization of molecules with the 3D ultrastructure of organelles. In this paper, we review the novel osmium maceration methods described earlier and discuss their potential and future directions in the field of biology and biomedical research.

    DOI： 10.1093/jmicro/dfad050

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    Other Link： https://academic.oup.com/jmicro/article-pdf/73/3/215/58122848/dfad050.pdf

  • Secretion Bias of Lamellar Granules Revealed by Three-Dimensional Electron Microscopy. Reviewed

    Ishida-Yamamoto A, Yamanishi H, Igawa S, Kishibe M, Kusumi S, Watanabe T, Koga D

    Journal of Investigative Dermatology   2023.7

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Secretion Bias of Lamellar Granules Revealed by Three-Dimensional Electron Microscopy Reviewed

    Akemi Ishida-Yamamoto, Haruyo Yamanishi, Satomi Igawa, Mari Kishibe, Satoshi Kusumi, Tsuyoshi Watanabe, Daisuke Koga

    Journal of Investigative Dermatology   143 ( 7 )   1310 - 1312.e3   2023.7

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    Authorship：Last author   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.jid.2023.03.1674

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  • The cholesterol pathway of the Golgi stress response induces cell death and transcription of Golgi-related genes through metabolic dysregulation of phosphatidylinositol-4-phosphate

    Kanae Sasaki, Takuya Adachi, Fumi Morishita, Marika Toide, Yuto Watanabe, Hajime Tajima Sakurai, Sadao Wakabayashi, Satoshi Kusumi, Toshiyuki Yamaji, Kaori Sakurai, Daisuke Koga, Kentaro Hanada, Masafumi Yohda, Hiderou Yoshida

    BioRxiv (Journal of Biological Chemistryでin press, 2025)   2023.5

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    Publisher：Cold Spring Harbor Laboratory  

    Abstract

    The Golgi stress response is an important cytoprotective system that enhances Golgi function in response to cellular demand, while cells damaged by prolonged Golgi stress undergo cell death to ensure the survival of organisms. OSW-1, a natural compound with anticancer activity, acts as a potent inhibitor of OSBP that transports cholesterol and phosphatidylinositol-4-phosphate (PI4P) at contact sites between the endoplasmic reticulum and the Golgi apparatus. Previously, we reported that OSW-1 induces the Golgi stress response, resulting in Golgi stress-induced transcription and cell death. However, the underlying molecular mechanism has been unknown. To reveal the mechanism of a novel pathway of the Golgi stress response regulating transcriptional induction and cell death (the cholesterol pathway), we performed a genome-wide knockout screen and found that transcriptional induction as well as cell death induced by OSW-1 was repressed in HeLa cells deficient in factors involved in the PI4P metabolism, such as PITPNB and PI4KB genes. Our data indicate that OSW-1 induces Golgi stress-dependent transcriptional induction and cell death through dysregulation of the PI4P metabolism in the Golgi apparatus.

    DOI： 10.1101/2023.05.18.541279

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  • A point mutation in GPI-attachment signal peptide accelerates the development of prion disease Reviewed

    Atsushi Kobayashi, Tetsuya Hirata, Taishi Shimazaki, Yoshiko Munesue, Keisuke Aoshima, Takashi Kimura, Junko Nio-Kobayashi, Rie Hasebe, Atsuko Takeuchi, Yuichi Matsuura, Satoshi Kusumi, Daisuke Koga, Yasushi Iwasaki, Taroh Kinoshita, Shirou Mohri, Tetsuyuki Kitamoto

    Acta Neuropathologica   2023.3

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    Language：English   Publishing type：Research paper (scientific journal)  

  • A point mutation in GPI-attachment signal peptide accelerates the development of prion disease Reviewed

    Atsushi Kobayashi, Tetsuya Hirata, Taishi Shimazaki, Yoshiko Munesue, Keisuke Aoshima, Takashi Kimura, Junko Nio-Kobayashi, Rie Hasebe, Atsuko Takeuchi, Yuichi Matsuura, Satoshi Kusumi, Daisuke Koga, Yasushi Iwasaki, Taroh Kinoshita, Shirou Mohri, Tetsuyuki Kitamoto

    Acta Neuropathologica   145 ( 5 )   637 - 650   2023.3

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00401-023-02553-5

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    Other Link： https://link.springer.com/article/10.1007/s00401-023-02553-5/fulltext.html

  • Monitoring of Post-Brain Injuries By Measuring Plasma Levels of Neuron-Derived Extracellular Vesicles Reviewed

    Naoshi Hotta, Takahiro Tadokoro, John Henry, Daisuke Koga, Keisuke Kawata, Hiroyuki Ishida, Yuko Oguma, Akihiro Hirata, Masato Mitsuhashi, Kenji Yoshitani

    Biomarker Insights   2022.10

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Polyvinyl alcohol coating prevents platelet adsorption and improves mechanical property of polycaprolactone-based small-caliber vascular graft Reviewed

    Naohiro Wakabayashi, Takumi Yoshida, Kyohei Oyama, Daisuke Naruse, Masahiro Tsutsui, Yuta Kikuchi, Daisuke Koga, Hiroyuki Kamiya

    Frontiers in Cardiovascular Medicine   2022.8

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Polyvinyl alcohol coating prevents platelet adsorption and improves mechanical property of polycaprolactone-based small-caliber vascular graft Reviewed

    Naohiro Wakabayashi, Takumi Yoshida, Kyohei Oyama, Daisuke Naruse, Masahiro Tsutsui, Yuta Kikuchi, Daisuke Koga, Hiroyuki Kamiya

    Frontiers in Cardiovascular Medicine   9   2022.8

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    Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    The low patency of synthetic vascular grafts hinders their practical applicability. Polyvinyl alcohol (PVA) is a non-toxic, highly hydrophilic polymer; thus, we created a PVA-coated polycaprolactone (PCL) nanofiber vascular graft (PVA–PCL graft). In this study, we examine whether PVA could improve the hydrophilicity of PCL grafts and evaluate its in vivo performance using a rat aorta implantation model. A PCL graft with an inner diameter of 1 mm is created using electrospinning (control). The PCL nanofibers are coated with PVA, resulting in a PVA–PCL graft. Mechanical property tests demonstrate that the PVA coating significantly increases the stiffness and resilience of the PCL graft. The PVA–PCL surface exhibits a much smaller sessile drop contact angle when compared with that of the control, indicating that the PVA coating has hydrophilic properties. Additionally, the PVA–PCL graft shows significantly less platelet adsorption than the control. The proposed PVA–PCL graft is implanted into the rat’s abdominal aorta, and its in vivo performance is tested at 8 weeks. The patency rate is 83.3% (10/12). The histological analysis demonstrates autologous cell engraftment on and inside the scaffold, as well as CD31/α-smooth muscle positive neointima regeneration on the graft lumen. Thus, the PVA–PCL grafts exhibit biocompatibility in the rat model, which suggests that the PVA coating is a promising approach for functionalizing PCL.

    DOI： 10.3389/fcvm.2022.946899

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  • Mutant GNAS limits tumor aggressiveness in established pancreatic cancer via antagonizing the KRAS-pathway. Reviewed

    Kawabata H, 他

    Journal of Gastroenterology   57 ( 3 )   208 - 220   2022.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00535-021-01846-4.

  • Mutant GNAS limits tumor aggressiveness in established pancreatic cancer via antagonizing the KRAS-pathway. Reviewed

    Hidemasa Kawabata, Yusuke Ono, Nobue Tamamura, Kyohei Oyama, Jun Ueda, Hiroki Sato, Kenji Takahashi, Kenzui Taniue, Tetsuhiro Okada, Syugo Fujibayashi, Akihiro Hayashi, Takuma Goto, Katsuro Enomoto, Hiroaki Konishi, Mikihiro Fujiya, Keita Miyakawa, Mishie Tanino, Yuji Nishikawa, Daisuke Koga, Tsuyoshi Watanabe, Chiho Maeda, Hidenori Karasaki, Andrew S Liss, Yusuke Mizukami, Toshikatsu Okumura

    Journal of gastroenterology   57 ( 3 )   208 - 220   2022.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    BACKGROUND: Mutations in GNAS drive pancreatic tumorigenesis and frequently occur in intraductal papillary mucinous neoplasm (IPMN); however, their value as a therapeutic target is yet to be determined. This study aimed at evaluating the involvement of mutant GNAS in tumor aggressiveness in established pancreatic cancer. METHODS: CRISPR/Cas9-mediated GNAS R201H silencing was performed using human primary IPMN-associated pancreatic cancer cells. The role of oncogenic GNAS in tumor maintenance was evaluated by conducting cell culture and xenograft experiments, and western blotting and transcriptome analyses were performed to uncover GNAS-driven signatures. RESULTS: Xenografts of GNAS wild-type cells were characterized by a higher Ki-67 labeling index relative to GNAS-mutant cells. Phenotypic alterations in the GNAS wild-type tumors resulted in a significant reduction in mucin production accompanied by solid with massive stromal components. Transcriptional profiling suggested an apparent conflict of mutant GNAS with KRAS signaling. A significantly higher Notch intercellular domain (NICD) was observed in the nuclear fraction of GNAS wild-type cells. Meanwhile, inhibition of protein kinase A (PKA) induced NICD in GNAS-mutant IPMN cells, suggesting that NOTCH signaling is negatively regulated by the GNAS-PKA pathway. GNAS wild-type cells were characterized by a significant invasive property relative to GNAS-mutant cells, which was mediated through the NOTCH regulatory pathway. CONCLUSIONS: Oncogenic GNAS induces mucin production, not only via MUC2 but also via MUC5AC/B, which may enlarge cystic lesions in the pancreas. The mutation may also limit tumor aggressiveness by attenuating NOTCH signaling; therefore, such tumor-suppressing effects must be considered when therapeutically inhibiting the GNAS pathway.

    DOI： 10.1007/s00535-021-01846-4

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  • Monitoring of Post-Brain Injuries By Measuring Plasma Levels of Neuron-Derived Extracellular Vesicles Reviewed

    Naoshi Hotta, Takahiro Tadokoro, John Henry, Daisuke Koga, Keisuke Kawata, Hiroyuki Ishida, Yuko Oguma, Akihiro Hirata, Masato Mitsuhashi, Kenji Yoshitani

    Biomarker Insights   17   2022.1

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    Publishing type：Research paper (scientific journal)   Publisher：SAGE Publications  

    Background:

    Extracellular vesicles (EV) released from neurons into the blood can reflect the state of nervous tissue. Measurement of neuron derived EV (NDE) may serve as an indicator of brain injury.

    Methods:

    A sandwich immunoassay was established to measure plasma NDE using anti-neuron CD171 and anti-EV CD9 ([CD171 <sup>+</sup> CD9<sup>+</sup>]). Plasma samples were obtained from commercial sources, cross-country (n = 9), football (n = 22), soccer (n = 19), and rugby (n = 18) athletes over time. Plasma was also collected from patients undergoing total aortic arch replacement (TAR) with selective cerebral perfusion during cardiopulmonary bypass before and after surgery (n = 36).

    Results:

    The specificity, linearity, and reproducibility of NDE assay (measurement of [CD171 <sup>+</sup> CD9<sup>+</sup>]) were confirmed. By scanning electron microscopy and nanoparticle tracking, spherical vesicles ranging in size from 150 to 300 nm were confirmed. Plasma levels of NDE were widely spread over 2 to 3 logs in different individuals with a significant age-dependent decrease. However, NDE were very stable in each individual within a ± 50% change over time (cross-country, football, soccer), whereas rugby players were more variable over 4 years. In patients undergoing TAR, NDE increased rapidly in days post-surgery and were significantly ( P = .0004) higher in those developing postoperative delirium (POD) (n = 13) than non-delirium patients (n = 23).

    Conclusions:

    The blood test to determine plasma levels of NDE was established by a sandwich immunoassay using 2 antibodies against neuron (CD171) and exosomes (CD9). NDE levels varied widely in different individuals and decreased with age, indicating that NDE levels should be considered as a normalizer of NDE biomarker studies. However, NDE levels were stable over time in each individual, and increased rapidly after TAR with greater increases associated with patients developing POD. This assay may serve as a surrogate for evaluating and monitoring brain injuries.

    DOI： 10.1177/11772719221128145

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    Other Link： https://journals.sagepub.com/doi/full-xml/10.1177/11772719221128145

  • Applications of Scanning Electron Microscopy Using Secondary and Backscattered Electron Signals in Neural Structure. Invited Reviewed

    Koga D, Kusumi S, Shibata M, Watanabe T

    Frontiers in Neuroanatomy   2021.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3389/fnana.2021.759804. eCollection 2021.

  • Applications of Scanning Electron Microscopy Using Secondary and Backscattered Electron Signals in Neural Structure Invited Reviewed

    Daisuke Koga, Satoshi Kusumi, Masahiro Shibata, Tsuyoshi Watanabe

    Frontiers in Neuroanatomy   15   2021.12

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    Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    Scanning electron microscopy (SEM) has contributed to elucidating the ultrastructure of bio-specimens in three dimensions. SEM imagery detects several kinds of signals, of which secondary electrons (SEs) and backscattered electrons (BSEs) are the main electrons used in biological and biomedical research. SE and BSE signals provide a three-dimensional (3D) surface topography and information on the composition of specimens, respectively. Among the various sample preparation techniques for SE-mode SEM, the osmium maceration method is the only approach for examining the subcellular structure that does not require any reconstruction processes. The 3D ultrastructure of organelles, such as the Golgi apparatus, mitochondria, and endoplasmic reticulum has been uncovered using high-resolution SEM of osmium-macerated tissues. Recent instrumental advances in scanning electron microscopes have broadened the applications of SEM for examining bio-specimens and enabled imaging of resin-embedded tissue blocks and sections using BSE-mode SEM under low-accelerating voltages; such techniques are fundamental to the 3D-SEM methods that are now known as focused ion-beam SEM, serial block-face SEM, and array tomography (i.e., serial section SEM). This technical breakthrough has allowed us to establish an innovative BSE imaging technique called section-face imaging to acquire ultrathin information from resin-embedded tissue sections. In contrast, serial section SEM is a modern 3D imaging technique for creating 3D surface rendering models of cells and organelles from tomographic BSE images of consecutive ultrathin sections embedded in resin. In this article, we introduce our related SEM techniques that use SE and BSE signals, such as the osmium maceration method, semithin section SEM (section-face imaging of resin-embedded semithin sections), section-face imaging for correlative light and SEM, and serial section SEM, to summarize their applications to neural structure and discuss the future possibilities and directions for these methods.

    DOI： 10.3389/fnana.2021.759804

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  • Thermoregulatory role of ghrelin in the induction of torpor under a restricted feeding condition Reviewed

    Takahiro Sato, Kanae Oishi, Daisuke Koga, Takanori Ida, Yusuke Sakai, Kenji Kangawa, Masayasu Kojima

    Scientific Reports   11 ( 1 )   2021.9

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    Abstract

    Ghrelin, a circulating orexigenic hormone secreted from the stomach, stimulates appetite and food intake by activating the hypothalamic arcuate nucleus. Administration of exogenous ghrelin exerts anabolic effects, causing weight gain, increased adiposity, and decreased metabolism. Body temperature (BT), which is determined by the balance of heat production and heat loss, must be strictly regulated to maintain proper cellular function and metabolism. However, the role of ghrelin in thermoregulation remains unclear. In this study, we found that ghrelin was essential for decreasing BT when mice are placed under calorie restriction. Elevated ghrelin concentrations induced by fasting correlated with significant decreases in BT, a hibernation-like state called torpor. Ghrelin-deficient (Ghrl<sup>−/−</sup>) animals could not enter torpor. The BT of Ghrl<sup>−/−</sup> mice also remained high under restricted feeding, but the animals gradually entered precipitous hypothermia, indicating thermoregulatory impairment. These effects of ghrelin on thermoregulation were the result of suppression of sympathetic nervous system activity input to brown adipose tissue; in the absence of ghrelin, it was not possible to suppress uncoupling protein 1 (ucp1) expression and decrease BT in low-energy states. Together, these findings demonstrate that ghrelin is an essential circulating hormone involved in lowering BT.

    DOI： 10.1038/s41598-021-97440-y

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    Other Link： https://www.nature.com/articles/s41598-021-97440-y

  • Novel concept for the epaxial/hypaxial boundary based on neuronal development. Reviewed International journal

    Hiroshi Nagashima, Daisuke Koga, Satoshi Kusumi, Katsuki Mukaigasa, Hiroyuki Yaginuma, Tatsuo Ushiki, Noboru Sato

    Journal of anatomy   237 ( 3 )   427 - 438   2020.9

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    Trunk muscles in vertebrates are classified as either dorsal epaxial or ventral hypaxial muscles. Epaxial and hypaxial muscles are defined as muscles innervated by the dorsal and ventral rami of spinal nerves, respectively. Each cluster of spinal motor neurons passing through dorsal rami innervates epaxial muscles, whereas clusters traveling on the ventral rami innervate hypaxial muscles. Herein, we show that some motor neurons exhibiting molecular profiles for epaxial muscles follow a path in the ventral rami. Dorsal deep-shoulder muscles and some body wall muscles are defined as hypaxial due to innervation via the ventral rami, but a part of these ventral rami has the molecular profile of motor neurons that innervate epaxial muscles. Thus, the epaxial and hypaxial boundary cannot be determined simply by the ramification pattern of spinal nerves. We propose that, although muscle innervation occurs via the ventral rami, dorsal deep-shoulder muscles and some body wall muscles represent an intermediate group that lies between epaxial and hypaxial muscles.

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  • 【「切片SEM法」の基礎と生物学・医学生物学研究への応用】切片SEM法と連続切片SEM法

    久住 聡, 甲賀 大輔, 柴田 昌宏, 渡部 剛

    顕微鏡   55 ( 1 )   18 - 22   2020.4

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  • β3-Adrenergic receptor blockade reduces mortality in endotoxin-induced heart failure by suppressing induced nitric oxide synthase and saving cardiac metabolism. Reviewed International journal

    Satoshi Kawaguchi, Motoi Okada, Eriko Ijiri, Daisuke Koga, Tsuyoshi Watanabe, Kentaro Hayashi, Yuta Kashiwagi, Satoshi Fujita, Naoyuki Hasebe

    American journal of physiology. Heart and circulatory physiology   318 ( 2 )   H283-H294   2020.2

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    The β3-adrenergic receptor (β3AR) is related to myocardial fatty acid metabolism and its expression has been implicated in heart failure. In this study, we investigated the role of β3AR in sepsis-related myocardial dysfunction using lipopolysaccharide (LPS)-induced endotoxemia as a model of cardiac dysfunction. We placed mice into three treatment groups and treated each with intraperitoneal injections of the β3AR agonist CL316243 (CL group), the β3AR antagonist SR59230A (SR group), or normal saline (NS group). Survival rates were significantly improved in the SR group compared with the other treatment groups. Echocardiography analyses revealed cardiac dysfunction within 6-12 h of LPS injections, but the outcome was significantly better for the SR group. Myocardial ATP was preserved in the SR group but was decreased in the CL-treated mice. Additionally, quantitative PCR analysis revealed that expression levels of genes associated with fatty acid oxidation and glucose metabolism were significantly higher in the SR group. Furthermore, the expression levels of mitochondrial membrane protein complexes were preserved in the SR group. Electron microscope studies showed significant accumulation of lipid droplets in the CL group. Moreover, inducible nitric oxide synthase (iNOS) protein expression and nitric oxide were significantly reduced in the SR group. The in vitro study demonstrated that β3AR has an independent iNOS pathway that does not go through the nuclear factor-κB pathway. These results suggest that blockading β3AR improves impaired energy metabolism in myocardial tissues by suppressing iNOS expression and recovers cardiac function in animals with endotoxin-induced heart failure.NEW & NOTEWORTHY Nitric oxide production through stimulation of β3-adrenergic receptor (β3AR) may improve cardiac function in cases of chronic heart failure. We demonstrated that the blockade of β3AR improved mortality and cardiac function in endotoxin-induced heart failure. We also determined that LPS-induced inducible nitric oxide synthase has a pathway that is independent of nuclear factor-κB, which worsened cardiac metabolism and mortality in the acute phase of sepsis. Treatment with the β3AR antagonist had a favorable effect. Thus, the blockade of β3AR could offer a novel treatment for sepsis-related heart failure.

    DOI： 10.1152/ajpheart.00108.2019

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  • Optimizing the reaction temperature to facilitate an efficient osmium maceration procedure. Reviewed

    Daisuke Koga, Satoshi Kusumi, Tsuyoshi Watanabe

    Biomedical research (Tokyo, Japan)   41 ( 4 )   161 - 168   2020

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    The osmium maceration method is a powerful technique for observing the three-dimensional ultrastructure of cellular organelles by scanning electron microscopy. In the conventional osmium maceration method, tissues are immersed in a diluted osmium tetroxide solution for several days at 20°C to remove soluble cytosolic proteins from the freeze-cracked surface of cells, and the optimal duration of this process is dependent on the cell type. To improve the efficiency of the osmium maceration procedure, we have examined systematically the relationship between the reaction temperature and time of the osmium maceration procedure. Treatment at temperatures higher than 20°C drastically shortened the time required to remove cytosolic proteins from the freeze-cracked surface of specimens with optimal durations for the osmium maceration of hepatocytes at 30, 40, 50 and 60°C being 30, 15, 5 and 1 h, respectively. Considering the stability and reproducibility of the macerated specimens, we concluded that the most appropriate temperature was 30 to 40°C. This rapid osmium maceration procedure was used successfully to observe the 3D ultrastructure of Purkinje cells in the cerebellum and proximal convoluted tubule cells in the kidney. This simple and reproducible rapid osmium maceration protocol should find wide appeal for the 3D analysis of cellular organelles in various cell types.

    DOI： 10.2220/biomedres.41.161

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  • 医学生物学分野におけるSEMの可能性

    甲賀大輔

    医生電顕技術誌   33   13 - 15   2020

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  • Culture in 10% O2 enhances the production of active hormones in neuro-endocrine cells by up-regulating the expression of processing enzymes Reviewed

    Sato E, Maeda Y, Sato Y, Hinata A, Gomi H, Koga D, Torii S, Watanabe T, Hosaka M

    Biochemical Journal   476   827 - 842   2019.3

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  • Three-dimensional configuration of apical epithelial compartments including stem cell niches in guinea pig cheek teeth Reviewed

    Seino Y, Nakatomi M, Ida-Yonemochi H, Koga D, Ushiki T, Ohshima H

    Journal of Oral Biosciences   61   55 - 63   2019.3

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  • Three-dimensional configuration of apical epithelial compartments including stem cell niches in guinea pig cheek teeth. Reviewed International journal

    Yuta Seino, Mitsushiro Nakatomi, Hiroko Ida-Yonemochi, Daisuke Koga, Tatsuo Ushiki, Hayato Ohshima

    Journal of oral biosciences   61 ( 1 )   55 - 63   2019.3

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    OBJECTIVES: Continuously growing rodent incisors have an apically located epithelial stem cell compartment, known as an "apical bud" (AB). Few studies have described the morphological features of ABs and stem cell niches in continuously growing premolars/molars. We attempted to clarify the relationship between the three-dimensional configuration of ABs and the stem cell niches in guinea pig cheek teeth. METHODS: We perfusion-fixed four-week-old guinea pigs, then decalcified their premolars/molars to produce serial paraffin sections, which we immunostained for Sox2. We reconstructed the serial sections using image processing and analysis software. We processed undecalcified samples for scanning electron microscopy by KOH digestion. RESULTS: Two types of epithelia with M and Δ shapes surrounded the S-shaped dental papilla in the apical region of the premolars/molars, and there were three Sox2-positive epithelial bulges above the M- and Δ-shaped epithelia. Sox2-positive epithelial stem cell niches were restricted to the apical side, and cell proliferation and differentiation immediately proceeded in the crown-analogue dentin. The Sox2-positive epithelial stem cell niches were sparsely distributed and extended to the occlusal side. We also detected continuously proliferating cells in the cervical loop and Hertwig's epithelial root sheath of the root-analogue dentin. CONCLUSIONS: Our findings suggest that guinea pig cheek teeth have three ABs, and the complex configuration of these types of teeth may be attributed to the prompt formation of crown-analogue dentin followed by the long-term formation of root-analogue dentin.

    DOI： 10.1016/j.job.2019.01.002

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  • The integral function of the endocytic recycling compartment is regulated by RFFL-mediated ubiquitylation of Rab11 effectors Reviewed

    Sato E, Maeda Y, Sato Y, Hinata A, Gomi H, Koga D, Torii S, Watanabe T, Hosaka M

    Journal of Cell Science   132   2019.2

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  • Culture in 10% O2 enhances the production of active hormones in neuro-endocrine cells by up-regulating the expression of processing enzymes. Reviewed

    Sato E, Maeda Y, Sato Y, Hinata A, Gomi H, Koga D, Torii S, Watanabe T, Hosaka M

    Biochem. J.   476   827 - 842   2019

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    DOI： 10.1042/BCJ20180832

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  • The integral function of the endocytic recycling compartment is regulated by RFFL-mediated ubiquitylation of Rab11 effectors. Reviewed International journal

    Sakai R, Fukuda R, Unida S, Aki M, Ono Y, Endo A, Kusumi S, Koga D, Fukushima T, Komada M, Okiyoneda T

    J. Cell Sci.   132 ( 3 )   pii: jcs228007   2019

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    Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role of ubiquitylation in lysosomal delivery has been well documented, but its role in the recycling pathway is largely unknown. Here, we report that the ubiquitin (Ub) ligase RFFL regulates ubiquitylation of endocytic recycling regulators. An RFFL dominant-negative (DN) mutant induced clustering of endocytic recycling compartments (ERCs) and delayed endocytic cargo recycling without affecting lysosomal traffic. A BioID RFFL interactome analysis revealed that RFFL interacts with the Rab11 effectors EHD1, MICALL1 and class I Rab11-FIPs. The RFFL DN mutant strongly captured these Rab11 effectors and inhibited their ubiquitylation. The prolonged interaction of RFFL with Rab11 effectors was sufficient to induce the clustered ERC phenotype and to delay cargo recycling. RFFL directly ubiquitylates these Rab11 effectors in vitro, but RFFL knockout (KO) only reduced the ubiquitylation of Rab11-FIP1. RFFL KO had a minimal effect on the ubiquitylation of EHD1, MICALL1, and Rab11-FIP2, and failed to delay transferrin recycling. These results suggest that multiple Ub ligases including RFFL regulate the ubiquitylation of Rab11 effectors, determining the integral function of the ERC.

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  • Impaired processing of prohormones in secretogranin III-null mice causes maladaptation to an inadequate diet and stress Reviewed

    Yoshinori Maeda, Saki Kudo, Ken Tsushima, Eri Sato, Chisato Kubota, Aika Kayamori, Hiroki Bochimoto, Daisuke Koga, Seiji Torii, Hiroshi Gomi, Tsuyoshi Watanabe, Masahiro Hosaka

    Endocrinology   159 ( 2 )   1213 - 1227   2018.2

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    DOI： 10.1210/en.2017-00636

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  • Differential effects of depot formulations of GnRH agonist leuprorelin and antagonist degarelix on the seminiferous epithelium of the rat testis Reviewed

    Hori J, Koga D, Kakizaki H, Watanabe T

    Biomedical Research   39   197 - 214   2018

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  • Backscattered electron imaging of resin-embedded sections. Invited Reviewed International journal

    Koga D, Kusumi S, Watanabe T

    Microscopy   67   196 - 206   2018

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    Scanning electron microscopes have longer focal depths than transmission electron microscopes and enable visualization of the three-dimensional (3D) surface structures of specimens. While scanning electron microscopy (SEM) in biological research was generally used for the analysis of bulk specimens until around the year 2000, more recent instrumental advances have broadened the application of SEM; for example, backscattered electron (BSE) signals under low accelerating voltages allow block-face and section-face images of tissues embedded in resin to be acquired. This technical breakthrough has led to the development of novel 3D imaging techniques including focused ion beam SEM, serial-block face SEM and serial section SEM. Using these new techniques, the 3D shapes of cells and cell organelles have been revealed clearly through reconstruction of serial tomographic images. In this review, we address two modern SEM techniques: section-face imaging of resin-embedded tissue samples based on BSE observations, and serial section SEM for reconstruction of the 3D structures of cells and organelles from BSE-mode SEM images of consecutive ultrathin sections on solid substrates.

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  • Combination of a cryosectioning method and section scanning electron microscopy for immuno-scanning electron microscopy Reviewed

    Satoshi Kusumi, Daisuke Koga, Tsuyoshi Watanabe, Masahiro Shibata

    Biomedical Research (Japan)   39 ( 1 )   21 - 25   2018

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Biomedical Research Foundation  

    DOI： 10.2220/biomedres.39.21

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  • Differential effects of depot formulations of GnRH agonist leuprorelin and antagonist degarelix on the seminiferous epithelium of the rat testis. Reviewed

    Hori J, Koga D, Kakizaki H, Watanabe T

    Biomed. Res.   39   197 - 214   2018

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    DOI： 10.2220/biomedres.39.197

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  • The ultrastructural characteristics of porcine hepatocytes donated after cardiac death and preserved with warm machine perfusion preservation Reviewed

    Hiroki Bochimoto, Naoto Matsuno, Yo Ishihara, Tatsuya Shonaka, Daisuke Koga, Yoshiki Hira, Yuji Nishikawa, Hiroyuki Furukawa, Tsuyoshi Watanabe

    PLOS ONE   12 ( 10 )   e0186352   2017.10

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    DOI： 10.1371/journal.pone.0186352

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  • Brachylaima ezohelicis sp nov (Trematoda: Brachylaimidae) found from the land snail Ezohelix gainesi, with a note of an unidentified Brachylaima species in Hokkaido, Japan Reviewed

    Minoru Nakao, Tsukasa Waki, Mizuki Sasaki, Jason L. Anders, Daisuke Koga, Mitsuhiko Asakawa

    PARASITOLOGY INTERNATIONAL   66 ( 3 )   240 - 249   2017.6

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    DOI： 10.1016/j.parint.2017.01.015

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  • Novel scanning electron microscopy methods for analyzing the 3D structure of the Golgi apparatus Invited Reviewed

    Daisuke Koga, Tatsuo Ushiki, Tsuyoshi Watanabe

    ANATOMICAL SCIENCE INTERNATIONAL   92 ( 1 )   37 - 49   2017.1

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    DOI： 10.1007/s12565-016-0380-8

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  • Changes in the three-dimensional ultrastructure of membranous organelles in male rat pituitary gonadotropes after castration Reviewed

    Daisuke Koga, Hiroki Bochimoto, Satoshi Kusumi, Tatsuo Ushiki, Tsuyoshi Watanabe

    BIOMEDICAL RESEARCH-TOKYO   38 ( 1 )   1 - 18   2017

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    DOI： 10.2220/biomedres.38.1

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  • Three-dimensional reconstruction of root cells and interdental cells in the rat inner ear by serial section scanning electron microscopy Reviewed

    Ryusuke Shodo, Manabu Hayatsu, Daisuke Koga, Arata Horii, Tatsuo Ushiki

    BIOMEDICAL RESEARCH-TOKYO   38 ( 4 )   239 - 248   2017

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    DOI： 10.2220/biomedres.38.239

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  • Integrative method for three-dimensional imaging of the entire Golgi apparatus by combining thiamine pyrophosphatase cytochemistry and array tomography using backscattered electron-mode scanning electron microscopy Reviewed

    Daisuke Koga, Satoshi Kusumi, Tatsuo Ushiki, Tsuyoshi Watanabe

    BIOMEDICAL RESEARCH-TOKYO   38 ( 5 )   285 - 296   2017

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    DOI： 10.2220/biomedres.38.285

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  • Direct evidence for activated CD8+T cell transmigration across portal vein endothelial cells in liver graft rejection Reviewed

    Taro Kariya, Hisashi Ueta, Xue-Dong Xu, Daisuke Koga, Taichi Ezaki, Enqiao Yu, Satoshi Kusumi, Yusuke Kitazawa, Yasushi Sawanobori, Tatsuo Ushiki, Thomas Issekutz, Kenjiro Matsuno

    JOURNAL OF GASTROENTEROLOGY   51 ( 10 )   985 - 998   2016.10

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    DOI： 10.1007/s00535-016-1169-1

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  • Backscattered electron image of osmium-impregnated/macerated tissues as a novel technique for identifying the cis-face of the Golgi apparatus by high-resolution scanning electron microscopy Reviewed

    D. Koga, H. Bochimoto, T. Watanabe, T. Ushiki

    JOURNAL OF MICROSCOPY   263 ( 1 )   87 - 96   2016.7

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    DOI： 10.1111/jmi.12379

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  • Three-dimensional shape of the Golgi apparatus in different cell types: serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus. Invited Reviewed

    Koga D, Kusumi S, Ushiki T

    Microscopy   65 ( 2 )   145 - 157   2016.4

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    DOI： 10.1093/jmicro/dfv360

  • Three-dimensional shape of the Golgi apparatus in different cell types: serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus Reviewed

    Daisuke Koga, Satoshi Kusumi, Tatsuo Ushiki

    MICROSCOPY   65 ( 2 )   145 - 157   2016.4

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    DOI： 10.1093/jmicro/dfv360

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  • Backscattered electron image of osmium-impregnated/macerated tissues as a novel technique for identifying the cis-faceof the Golgi apparatus by high-resolution scanning electron microscopy. Reviewed

    Koga D, Bochimoto H, Watanabe T and Ushiki T

    Journal of Microscopy   in press   2016

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    DOI： 10.1111/jmi.12379

  • Correlative Light and Scanning Electron Microscopy for Observing the Three-Dimensional Ultrastructure of Membranous Cell Organelles in Relation to Their Molecular Components Reviewed

    Daisuke Koga, Satoshi Kusumi, Hiroki Bochimoto, Tsuyoshi Watanabe, Tatsuo Ushiki

    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY   63 ( 12 )   968 - 979   2015.12

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    DOI： 10.1369/0022155415609099

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  • High-resolution imaging by scanning electron microscopy of semithin sections in correlation with light microscopy Reviewed

    Daisuke Koga, Satoshi Kusumi, Ryusuke Shodo, Yukari Dan, Tatsuo Ushiki

    MICROSCOPY   64 ( 6 )   387 - 394   2015.12

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    DOI： 10.1093/jmicro/dfv042

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  • Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen Reviewed

    Satoshi Kusumi, Daisuke Koga, Tatsuo Kanda, Tatsuo Ushiki

    BIOMEDICAL RESEARCH-TOKYO   36 ( 3 )   195 - 203   2015.6

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    DOI： 10.2220/biomedres.36.195

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  • Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen. Reviewed

    Kusumi S, Koga D, Kanda T and Ushiki T

    Biomed Research   36   195 - 203   2015

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  • Correlative Light and Scanning Electron Microscopy for Observing the Three-Dimensional Ultrastructure of Membranous Cell Organelles in Relation to their Molecular Components. Reviewed

    Koga D, Kusumi S, Bochimoto H, Watanabe T, Ushiki T

    Journal of Histochemistry and Cytochemistry   63   968 - 979   2015

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    DOI： 10.1369/0022155415609099

  • High resolution imaging by scanning electron microscopy of semithin sections in correlation with light microscopy. Invited Reviewed

    Koga D, Kusumi S, Shodo R, Dan Y and Ushiki T

    Microscopy   64   387 - 394   2015

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  • Functional implications of the Golgi and microtubular network in gonadotropes Reviewed

    Tsuyoshi Watanabe, Hiroki Bochimoto, Daisuke Koga, Masahiro Hosaka, Tatsuo Ushiki

    MOLECULAR AND CELLULAR ENDOCRINOLOGY   385 ( 1-2 )   88 - 96   2014.3

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    DOI： 10.1016/j.mce.2013.10.003

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  • Functional Implications of the Golgi and Microtubular Network in Gonadotropes. Invited Reviewed

    Watanabe T, Bochimoto H, Koga D, Hosaka M and Ushiki T

    Molecular and Cellular Endocrinology.   385   88 - 96   2014

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    DOI： 10.1016/j.mce.2013.10.003

  • 連続切片SEM法とゴルジ装置の3D構造解析への応用 Invited Reviewed

    甲賀大輔, 久住聡, 牛木辰男

    顕微鏡   49   171 - 175   2014

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    DOI： 10.11410/kenbikyo.49.3_171

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  • Three-Dimensional Morphology of Touch Domes in Human Hairy Skin by Correlative Light and Scanning Electron Microscopy Reviewed

    Mari Orime, Tatsuo Ushiki, Daisuke Koga, Masaaki Ito

    JOURNAL OF INVESTIGATIVE DERMATOLOGY   133 ( 8 )   2108 - 2111   2013.8

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  • Sustained treatment with a GnRH agonist (Leuprorelin) affects the ultrastructural characteristics of membranous organelles in male rat pituitary gonadotropes Reviewed

    Hiroki Bochimoto, Daisuke Koga, Yuko Sakai, Yoshiki Hira, Masahiro Hosaka, Tatsuo Ushiki, Tsuyoshi Watanabe

    Archives of Histology and Cytology   74 ( 1 )   41 - 57   2013.7

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    DOI： 10.1679/aohc.74.41

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  • Three-dimensional ultra-structures of myelin and the axons in the spinal cord: Application of SEM with the osmium maceration method to the central nervous system in two mouse models Reviewed

    Taichi Nomura, Yoshio Bando, Hiroki Bochimoto, Daisuke Koga, Tsuyoshi Watanabe, Shigetaka Yoshida

    Neuroscience Research   75 ( 3 )   190 - 197   2013.3

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    DOI： 10.1016/j.neures.2013.01.009

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  • Three-Dimensional Morphology of Touch Domes in Human Hairy Skin by Correlative Light and Scanning Electron Microscopy. Reviewed

    Orime M, Ushiki T, Koga D and Ito M

    Journal of investigative Dermatology   133   2108 - 2111   2013

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  • Sustained treatment with a GnRH agonist (leuprorelin) affects the ultrastructural characteristics of membranous organelles in male rat pituitary gonadotropes. Reviewed

    Bochimoto H, Koga D, Sakai Y, Hira Y, Hosaka M, Ushiki T and Watanabe T

    Archives of Histology and Cytology   74   41 - 57   2013

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    DOI： 10.1679/aohc.74.41

  • Three-dimensional ultra-structures of myelin and the axons in the spinal cord: Application of SEM with the osmium maceration method to the central nervous system in two mouse models. Reviewed

    Nomura T, Bando Y, Bochimoto H, Koga D, Watanabe T, Yoshida S

    Neuroscience Research   75   190 - 197   2013

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    DOI： 10.1016/j.neures.2013.01.009

  • Morphological assessment of early axonal regeneration in end-to-side nerve coaptation models Reviewed

    Hiroshi Oyamatsu, Daisuke Koga, Michihiro Igarashi, Minoru Shibata, Tatsuo Ushiki

    JOURNAL OF PLASTIC SURGERY AND HAND SURGERY   46 ( 5 )   299 - 307   2012.10

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    DOI： 10.3109/2000656X.2012.696264

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  • Tetraspanin Is Required for Generation of Reactive Oxygen Species by the Dual Oxidase System in Caenorhabditis elegans Reviewed

    Hiroki Moribe, Ryouji Konakawa, Daisuke Koga, Tatsuo Ushiki, Kuniaki Nakamura, Eisuke Mekada

    PLOS GENETICS   8 ( 9 )   e1002957   2012.9

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    DOI： 10.1371/journal.pgen.1002957

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  • A Unique Ball-Shaped Golgi Apparatus in the Rat Pituitary Gonadotrope: Its Functional Implications in Relation to the Arrangement of the Microtubule Network Reviewed

    Tsuyoshi Watanabe, Yuko Sakai, Daisuke Koga, Hiroki Bochimoto, Yoshiki Hira, Masahiro Hosaka, Tatsuo Ushiki

    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY   60 ( 8 )   588 - 602   2012.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1369/0022155412448791

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  • A useful method for observing intracellular structures of free and cultured cells by scanning electron microscopy Reviewed

    Daisuke Koga, Masato Nakajima, Tatsuo Ushiki

    JOURNAL OF ELECTRON MICROSCOPY   61 ( 2 )   105 - 111   2012.4

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/jmicro/dfr098

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  • リアルタイム3D走査電子顕微鏡の医学生物応用 Invited Reviewed

    牛木辰男, 伊東祐博, 伊藤広, 岩田太, 甲賀大輔

    顕微鏡   45   198 - 201   2010

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  • Trafficking of recirculating lymphocytes in the rat liver: Rapid transmigration into the portal area and then to the hepatic lymph Reviewed

    Xue-Dong Xu, Hisashi Ueta, Shu Zhou, Changde Shi, Daisuke Koga, Tatsuo Ushiki, Kenjiro Matsuno

    LIVER INTERNATIONAL   28 ( 3 )   319 - 330   2008.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1478-3231.2008.01671.x

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  • 走査電子顕微鏡によるゴルジ装置の形態解析 Invited Reviewed

    甲賀大輔, 牛木辰男

    顕微鏡   43   283 - 286   2008

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  • Notes on ovarian histology of a harbor seal, Phoca largha, stranded on the beach at Izumozaki, Niigata Prefecture, Sea of Japan. Reviewed

    Honma Y, Koga D, Ushiki T, Aoyagi A, Shindo J

    J. Jap. Drif. Soc.   5   11 - 17   2007

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  • Three-dimensional ultrastructure of the Golgi apparatus in different cells: high-resolution scanning electron microscopy of osmium-macerated tissues Reviewed

    Daisuke Koga, Tatsuo Ushiki

    ARCHIVES OF HISTOLOGY AND CYTOLOGY   69 ( 5 )   357 - 374   2006.12

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1679/aohc.69.357

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  • SEM observation of the insect prepared by microwave irradiation Reviewed

    D Koga, M Ueno, S Yamashina

    JOURNAL OF ELECTRON MICROSCOPY   52 ( 5 )   477 - 484   2003

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/jmicro/52.5.477

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Books

  • The Golgi network : Golgi organization and dynamics

    Sengupta, Kaushik, Muchir, Antoine（ Role： Contributor）

    Springer Nature Swizerland  2026  （ ISBN:9783032069351 ）

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    Total pages：x, 358 pages   Language：English  

    CiNii Books

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  • 走査電子顕微鏡

    日本表面真空学会

    丸善出版  2025.12  （ ISBN:9784621312278 ）

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    Total pages：x, 212p   Language：Japanese  

    CiNii Books

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  • 新 電顕入門ガイドブック

    [公社]日本顕微鏡学会 電子顕微鏡技術認定委員会（ Role： Joint author）

    国際文献社  2022.6  （ ISBN:4910603050 ）

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    Language：Japanese   Book type：Textbook, survey, introduction

  • 新電顕入門ガイドブック

    日本顕微鏡学会電子顕微鏡技術認定委員会（ Role： Contributor）

    国際文献社  2022.5  （ ISBN:9784910603056 ）

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    Total pages：xl, 248p   Language：Japanese  

    CiNii Books

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  • ミクロにひそむ不思議

    牛木辰男, 甲賀大輔

    岩波書店  2008 

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  • 細胞生物学

    牛木辰男, 堅田利明, 甲賀大輔, 木南凌, 佐々木啓子, 白土明子, 高橋悟, 中西義信, 松岡耕二, 光本篤史, 堅田利明

    廣川書店 

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MISC

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Presentations

  • 電顕によるオルガネライメージングのニューエッジ International conference

    甲賀 大輔

    第45回日本分子生物学会年会  

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    Event date： 2022.11 - 2022.12

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

  • 脳の細胞内外の構造を超解像可視化技術で明らかにする

    Daisuke Koga

    NEURO2022 

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    Event date： 2022.6 - 2022.7

    Language：English   Presentation type：Symposium, workshop panel (public)  

  • Recent applications of correlative light and electron microscopy

    Daisuke Koga, Satoshi Kusumi, Tsuyoshi Watanabe

    日本顕微鏡学会　第78回学術集会 

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    Event date： 2022.5

    Language：English   Presentation type：Symposium, workshop panel (public)  

  • SEM によるオルガネラの3D 構造解析

    甲賀大輔　久住聡　渡部剛

    第127回日本解剖学会総会・全国学術集会 

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    Event date： 2022.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  • 連続切片SEM法によるゴルジ装置の3D構造解析

    甲賀大輔

    生体機能ボリュームデータ解析研究部会 第６回研究会 

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    Event date： 2022.3

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

  • オルガネラや細胞膜の特殊化した膜領域「ゾーン」研究の最前線

    甲賀 大輔, 久住 聡, 森永 涼介, 渡部 剛

    第128回日本解剖学会総会　学術集会 

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    Event date： 2022.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  • 走査電子顕微鏡法 (SEM法) -オルガネラ3D形態解析を目指して-

    甲賀大輔

    第44回日本分子生物学会年会  

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    Event date： 2021.12

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

  • オルガネラや細胞膜の特殊化した膜領域「ゾーン」研究の最前線 （日本生理学会連携シンポジウム)

    甲賀 大輔 、久住 聡 、森永 涼介 、渡部 剛

    第128回日本解剖学会総会・全国学術集会 

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    Event date： 2023.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：東北大学  

  • 細胞内スーパーイメージングの最先端

    甲賀大輔, 渡部剛, 中澤英子, 内山安男

    第124回日本解剖学会・全国学術集会 

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    Event date： 2019.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  • 「医学生物学領域における SEM のこれまでとこれから」

    甲賀大輔

    顕微鏡学会関東支部講演会 

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    Event date： 2019.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：順天堂大学本郷キャンパス  

  • 「医学・生物学研究におけるSEMの応用」

    甲賀大輔, 渡部剛, 中澤英子, 内山安男

    形態解析ワークショップ‐多様な顕微鏡を用いて 

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    Event date： 2019.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：品川インターシティ  

  • 細胞内スーパーイメージングの世界

    甲賀大輔, 久住聡, 渡部剛

    第41回日本分子生物学会年会 

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    Event date： 2018.11

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：パシフィコ横浜  

  • 連続切片のSEM観察

    甲賀　大輔

    SCANTECH2018  東京都市大学

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    Event date： 2018.8

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  • 下垂体研究の未来と展望

    甲賀大輔, 久住聡, 渡部剛

    下垂体研究会 

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    Event date： 2018.8

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：国民宿舎「桂浜荘」  

  • Microscopy冠ワークショップ受賞講演

    甲賀大輔, 久住聡, 牛木辰男

    第74回日本顕微鏡学会学術講演会 

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    Event date： 2018.5

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：久留米シティプラザ  

  • Tokuyasu Method：An Old and New Technique International conference

    Daisuke Koga, Satoshi Kusumi, Tsuyoshi Watanabe

    第74回日本顕微鏡学会学術講演会  久留米シティプラザ

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    Event date： 2018.5

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

    Venue：久留米シティプラザ  

  • 基礎技術チュートリアル

    甲賀大輔

    第74回日本顕微鏡学会学術講演会 

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    Event date： 2018.5

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  • 先端的3Dイメージング技法によるゴルジ装置の形態的多様性の観察

    甲賀大輔, 久住聡, 暮地本宙己, 牛木辰男, 渡部剛

    第121回日本解剖学会総会・全国学術集会 

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    Event date： 2016.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：ビックパレふくしま  

  • 準超薄切片の超薄像観察法の開発と応用

    甲賀大輔, 久住聡, 暮地本宙己, 渡部剛

    平成27年度日本顕微鏡学会北海道支部講演会 

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    Event date： 2015.12

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

  • 凍結割断による生物試料の観察

    甲賀　大輔

    SCANTECH 2015 

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    Event date： 2015.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  • 蛍光免疫染色像とオスミウム浸軟SEM像の相関顕微鏡法の開発

    甲賀大輔, 久住聡, 暮地本宙己, 渡部剛, 牛木辰男

    第61回東北・北海道連合支部学術集会 

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    Event date： 2015.8

    Language：Japanese   Presentation type：Oral presentation (general)  

  • 連続切片走査電子顕微鏡・3D再構築法によるゴルジ装置の立体構造解析

    甲賀大輔, 久住聡, 牛木辰男

    第71回日本解剖学会学術講演会 

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    Event date： 2015.5

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  • 徳安凍結切片法とオスミウム浸軟法を組み合わせた免疫蛍光・走査電子顕微鏡相関法 Invited

    甲賀大輔

    第67回顕微鏡学会シンポジウム  2024.11 

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    Presentation type：Symposium, workshop panel (nominated)  

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  • オスミウム浸軟法によるオルガネラの3D構造解析

    甲賀大輔

    第47回日本分子生物学会年会  2024.11 

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    Presentation type：Symposium, workshop panel (public)  

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  • The shape of mitochondria analyzed by ultra-high resolution scanning electron microscopy International conference

    Koga D, Ushiki T

    16th International Congress of the IFAA  2004.8 

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  • Three-dimensional ultrastructure of the Golgi apparatus in different cells: high-resolution scanning electron microscopy of osmium-macerated tissues Invited International conference

    Koga D, Ushiki T

    The16th International Microscopy Congress  2006.9 

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    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • 走査電子顕微鏡によるゴルジ装置の極性観察－オスミウム染色法とその応用例－

    甲賀大輔, 牛木辰男

    第63回日本顕微鏡学会総会  2007.5 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Backscattered electron imaging of osmium impregnated macerated tissues as a novel technique for identifying the cis-face of the Golgi apparatus by high-resolution scanning electron microscopy International conference

    Koga D, Ushiki T

    XX International Symposium on Morphological Sciences  2008.9 

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  • バイオ研究におけるSEMの活用 Invited

    甲賀大輔, 牛木辰男

    SCANTECH2009  2009.9 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • Morphological change of membranous cell organelles in the gonadotroph of the rat anterior pituitary gland after castration International conference

    Koga D, Bochimoto H, Watanabe T, Ushiki T

    XXI International Symposium on Morphological Sciences  2010.9 

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  • ゴルジ装置の多様な形態と機能変化－走査電顕による立体構造解析－

    甲賀大輔, 暮地本宙己, 渡部剛, 牛木辰男

    第115回日本解剖学会学術総会  2010.3 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • 走査電子顕微鏡による海綿体の立体構造解析 Invited

    甲賀大輔

    第24回日本抗加齢医学会総会  2024.6 

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    Presentation type：Symposium, workshop panel (public)  

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  • 超高分解能SEMによる膜性小器官の観察

    甲賀大輔, 渡部剛, 中澤英子, 内山安男

    第124回日本解剖学会総会・全国学術集会  2019 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • XXI International Symposium on Morphological Sciences International conference

    Koga D, Ushiki T

    XXII International Symposium on Morphological Sciences  2012 

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  • Correlative light and scanning electron microscopy for observing the three-dimensional ultrastructure of membranous cell organelles in relation to their molecular components International conference

    Koga D, Kusumi S, Bochimoto H, Watanabe T, Ushiki T

    XXIII International Symposium on Morphological Sciences  2013 

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    Language：English   Presentation type：Oral presentation (general)  

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  • バイオ研究におけるSEMの応用 Invited

    SCANTECH 2014  2014.9 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • 連続切片走査電子顕微鏡法・3D再構築法によるゴルジ装置の立体構造解析 Invited

    甲賀大輔, 久住聡, 牛木辰男

    第70回日本顕微鏡学会学術総会  2014.5 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • 蛍光イメージングと走査電子顕微鏡像の対比法 Invited

    甲賀大輔, 久住聡, 暮地本宙己, 渡部剛, 牛木辰男

    第70回日本顕微鏡学会学術総会  2014.5 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • 走査電子顕微鏡による下垂体前葉細胞の3D微細構造解析 Invited

    甲賀大輔, 牛木辰男

    第18回日本内分泌病理学会学術総会  2014.11 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Serial section scanning electron microscopy and its application for the morphological analysis of the Golgi apparatus Invited

    Koga D, Kusumi S, Ushiki T

    第120回日本解剖学会総会･全国学術集会・第92回 日本生理学会大会合同大会  2015.3 

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  • 凍結割断による生物試料の観察 Invited

    Daisuke Koga

    2015 

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  • 連続切片走査電子顕微鏡・3D再構築法によるゴルジ装置の立体構造解析 Invited

    甲賀大輔, 久住聡, 暮地本宙己, 渡部剛, 牛木辰男

    第71回日本顕微鏡学会総会  2015.5 

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  • 走査電子顕微鏡試料作製法

    甲賀大輔

    第72回日本顕微鏡学会学術講演会  2016 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • 医学生物学分野におけるSEMの可能性 Invited

    甲賀 大輔

    医学生物学電子顕微鏡技術学会第35回学術講演会  2019 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Three-dimensional structure of the Golgi apparatus in mammalian cells

    Daisuke Koga, Satoshi Kusumi, Tsuyoshi Watanabe

    2019 

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  • 切片SEM法

    甲賀 大輔, 久住 聡, 渡部 剛

    第75回日本顕微鏡学会学術講演会  2019 

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  • 最先端の3Dイメージング技法によるゴルジ装置の形態学的多様性の観察

    甲賀大輔, 久住聡, 暮地本宙己, 牛木辰男, 渡部剛

    第121回日本解剖学会総会・全国学術集会  2016 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • SEM試料作製法の基礎

    甲賀大輔

    第73回日本顕微鏡学会学術講演会  2017 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • 免疫組織化学染色切片とオスミウム浸軟組織の相関顕微鏡法 Invited International conference

    甲賀大輔, 久住聡, 渡部剛

    第73回日本顕微鏡学会学術講演会  2017 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • SEM試料作製法

    甲賀大輔

    第74回日本顕微鏡学会学術講演会  2018 

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  • 連続切片SEM・3D再構築法 Invited

    甲賀大輔

    SCANTECH 2018  2018 

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  • 細胞内膜系のダイナミクス

    甲賀大輔, 渡部剛, 中澤英子, 内山安男

    第123回日本解剖学会総会・全国学術集会  2018 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • 超高分解能SEMによる細胞内膜系の3Dイメージング

    甲賀大輔, 渡部剛, 中澤英子, 内山安男

    第41回日本分子生物学会年会  2018 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Three-dimensional shape of the Golgi apparatus in different cell types:serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus

    甲賀大輔, 久住聡, 牛木辰男

    第74回日本顕微鏡学会学術講演会 (Microscopy冠ワークショップ, 論文賞受賞講演)  2018 

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  • Correlative light and scanning electron microscopy (CLSEM) by combining Tokuyasu cryosectioning technique with osmium maceration method. Invited

    2018 

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  • 新規3D電顕技法によるゴルジ装置の形態基盤構築

    甲賀大輔, 久住聡, 森永涼介, 渡部剛

    第70回日本解剖学会東北・北海道連合支部学術集会  2024.9 

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  • 走査電子顕微鏡による下垂体前葉オルガネラの3Dイメージングに関する研究

    甲賀大輔

    第39回日本下垂体研究会学術集会  2024.8 

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    Presentation type：Oral presentation (invited, special)  

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  • 走査電子顕微鏡でみたゴルジ装置 －その多様な形態と謎－ Invited

    甲賀大輔, 牛木辰男

    第3回可視化技術ワークショップ  2006.11 

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    Presentation type：Symposium, workshop panel (nominated)  

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  • 走査電子顕微鏡によるオルガネラの3D微細構造解析 Invited

    甲賀大輔

    第59回日本脳炎ウイルス生態学研究会  2025.5 

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    Presentation type：Oral presentation (invited, special)  

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  • The Golgi apparatus and scanning electron microscopy Invited

    Koga D.

    2025.7 

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    Presentation type：Symposium, workshop panel (public)  

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  • Daisuke KOGA;Satoshi KUSUMI;Takahiro SATO Invited

    甲賀大輔, 久住聡  2025.9 

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    Presentation type：Symposium, workshop panel (nominated)  

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  • Daisuke KOGA;Satoshi KUSUMI;Takahiro SATO Invited

    Daisuke KOGA;Satoshi KUSUMI;Takahiro SATO  2025.8 

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    Presentation type：Symposium, workshop panel (nominated)  

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  • 3D morphological imaging of the Golgi apparatus by SEM Invited

    Koga D Kusumi S.

    Daisuke KOGA;Satoshi KUSUMI;Takahiro SATO  2025.7 

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    Presentation type：Symposium, workshop panel (public)  

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Awards

  • 吉村賞

    2024.8   日本下垂体研究会  

    甲賀 大輔

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  • 日本解剖学会 第68回東北・北海道連合支部学術集会学会賞

    2022.9   日本解剖学会 第68回東北・北海道連合支部  

    甲賀 大輔, 久住 聡, 渡部 剛

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  • 学会賞

    2022.9   日本解剖学会東北・北海道連合支部  

    甲賀 大輔

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  • 日本解剖学会 東北北海道連合支部学術集会 優秀発表賞 受賞

    2019.9   日本解剖学会  

    甲賀大輔　久住聡　渡部剛

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  • 優秀発表賞

    2019.9   日本解剖学会 東北北海道連合支部学術集会  

    甲賀 大輔

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    Award type：Award from Japanese society, conference, symposium, etc. 

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  • Microscopy論文賞

    2018.5   日本顕微鏡学会  

    甲賀大輔

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    Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

  • 論文賞

    2018.5   Microscopy (Oxford)  

    甲賀大輔

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    Award type：Honored in official journal of a scientific society, scientific journal 

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  • 日本顕微鏡学会　奨励賞(生物系応用研究部門)

    2016.6   日本顕微鏡学会  

    甲賀　大輔

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    Award type：International academic award (Japan or overseas)  Country：Japan

  • 奨励賞

    2016.5   日本顕微鏡学会  

    甲賀大輔

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  • 日本解剖学会奨励賞

    2016.3   日本顕微鏡学会  

    甲賀　大輔

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  • 奨励賞

    2016.3   日本解剖学会  

    甲賀大輔

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  • ポスター賞

    2015.2   第39回日本顕微鏡学会 関東支部講演会  

    甲賀大輔

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    Award type：Award from Japanese society, conference, symposium, etc. 

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  • 優秀発表賞

    2012.8   第27回日本下垂体研究会学術集会  

    甲賀大輔

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    Award type：Award from Japanese society, conference, symposium, etc. 

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  • Best Scientific Poster

    2012.2  

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    Award type：Award from international society, conference, symposium, etc. 

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  • “Pietro Motta” Award

    2010.9  

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    Award type：Award from international society, conference, symposium, etc. 

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  • ポスター賞

    2009.5   第65回日本顕微鏡学会学術総会  

    甲賀大輔

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    Award type：Award from Japanese society, conference, symposium, etc. 

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  • 市民選出最優秀賞

    日本顕微鏡学会写真コンクール(日本顕微鏡学会第63回学術講演会)  

    甲賀大輔

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    Award type：Award from Japanese society, conference, symposium, etc. 

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Research Projects

  • Uncovering the selective sorting program in the lysosome transport pathway

    Grant number：25K02410  2025.4 - 2029.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Grant amount：\18,720,000 （ Direct Cost: \14,400,000 、 Indirect Cost：\4,320,000 ）

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  • iPS細胞を用いたSIFDの病態解明と治療法開発のための基盤研究

    2024.4 - 2027.3

    基盤研究(C)

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    Sideroblastic anemia with B-cell immunodeficiency, fevers, and developmental delay (SIFD)の病態解明を目指した基盤研究。

  • 独創的3D電顕イメージングで探る神経細胞ゴルジ体の集合化と分散化のメカニズム

    2024.4 - 2027.3

    基盤研究(C)

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    神経細胞Golgi体の基本的構造を解明し、その構造学的知見を基に、生後発達過程における神経細胞Goli体の高次構造獲得過程と、病態時のGolgi体の崩壊メカニズムの解明を目指した研究。

  • Research for the Pathological Understanding and Therapeutic Development of SIFD Using iPS Cells

    Grant number：24K11037  2024.4 - 2027.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4,680,000 （ Direct Cost: \3,600,000 、 Indirect Cost：\1,080,000 ）

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  • Elucidating the mechanism of assembly and disassembly of the Golgi apparatus in neurons using 3D electron microscopy techniques.

    Grant number：24K09993  2024.4 - 2027.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4,550,000 （ Direct Cost: \3,500,000 、 Indirect Cost：\1,050,000 ）

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  • リポソームを捕捉したマクロファージの MDSC 様細胞への変容に関わる分子基盤の解明

    2023.4 - 2026.3

    基盤研究(C)

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    マクロファージに取り込まれたリポソームがどのような細胞内シグナル変化を引き起こし骨髄由来免疫抑制細胞(MDSC)誘導に至るのかを解明する研究。

  • リポソームを捕捉したマクロファージのMDSC様細胞への変容に関わる分子基盤の解明

    Grant number：23K07243  2023.4 - 2026.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    吉田 陽一郎, 東 寛, 佐藤 雅之, 甲賀 大輔, 長森 恒久, 青山 藍子, 酒井 宏水, 石羽澤 映美

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    Grant amount：\4,160,000 （ Direct Cost: \3,200,000 、 Indirect Cost：\960,000 ）

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  • フラビウイルス蛋白質量恒常性維持の分子機構解明

    2022.4 - 2026.3

    基盤研究(B)

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    デングウイルス(DENV)や日本脳炎ウイルス(JEV)などのフラビウイルスは、宿主の小胞体関連分解 (endoplasmic reticulum (ER) associated degradation (ERAD)) システムによって、各ウイルス蛋白質の量比が制御されている。このシステムにおいて、どのように成熟した非構造蛋白質が選択的に分解されるのか、または分解阻害に伴うウイルス増殖抑制の分子機構などいくつかの不明な点が残されている。
    本研究では、どのような機構により一部のウイルス因子のみがERADによって選択的分解を受けるのか？不要蛋白質の蓄積がなぜウイルスの複製を抑制するのか？
    類似の機構は細胞に備わっているのか？など、まだ明らかにされていない部分について解析を進め、新たな抗ウイルス戦略開発に繋がる分子基盤の解明を目指す。

  • フラビウイルス蛋白質量恒常性維持の分子機構解明

    Grant number：22H02873  2022.4 - 2026.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    森田 英嗣, 甲賀 大輔

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    Grant amount：\17,160,000 （ Direct Cost: \13,200,000 、 Indirect Cost：\3,960,000 ）

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  • フラビウイルス蛋白質量恒常性維持の分子機構解明

    Grant number：23K24135  2022.4 - 2026.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    森田 英嗣, 甲賀 大輔

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    Grant amount：\17,160,000 （ Direct Cost: \13,200,000 、 Indirect Cost：\3,960,000 ）

    研究項目1. 一部のウイルス非構造蛋白質のみが選択的にERADで分解される分子機構の解明：日本脳炎ウイルスのEタンパク質、prMタンパク質とNS1タンパク質の糖鎖修飾を欠損させた変異体の安定性が著しく減弱する要因として、小胞体関連分解ERADが重要な役割を担っていることを明らかにした。
    研究項目2. ウイルス感染細胞内でERAD因子が集積するコンボリューティッド膜(CM)の解析：日本脳炎ウイルス感染細胞内に形成されるCMの脂質組成の解析を進め、コレステロールが多く蓄積していること、また、セラミドなど一部の脂質が排除されていることを明らかにした。また、タイムラプスイメージング解析によりウイルス複製オルガネラ形成過程を捉えることに成功した。
    研究項目3. ２種類の異なるCM構造の役割と形成・維持機構の解明： CM膜構造の3D走査型電子顕微鏡（SEM）イメージの取得に成功した。また、タイムラプスイメージングと光電子相関顕微鏡(CLEM)を組み合わせた動態三次元電子顕微鏡解析に成功した。
    研究項目4.他のストレス誘導時に見られるCM様構造との比較解析(ウイルス因子のViroporinとしての役割) ： Fura 2-AM を用いた カルシウムイオン濃度変化測定 の条件検討を進め、一部ウイルス非構造タンパク質発現がカルシウムチャネル活性を強く抑制することを明らかにした。

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  • ゴルジ体の動態解明に基づく糖鎖修飾の制御

    2021.10 - 2027.3

    戦略的創造研究推進事業 (JST CREST)

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    糖鎖修飾の舞台としてのゴルジ体に着目し、その微細構造の時空間ダイナミクスと糖タンパク質の輸送経路を精密探査するものである。さらに、ゴルジ体の形成とカーゴ分子の選別輸送の分子機構を解明することにより、分泌経路の設計原理を理解し、その過程で得られた知見を基にタンパク質の糖鎖修飾を制御することを目指す。

  • Glycosylation machinery regulated by localization of glycosyltransferases and selective transport of substrate proteins

    Grant number：23K21358  2021.4 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Grant amount：\17,290,000 （ Direct Cost: \13,300,000 、 Indirect Cost：\3,990,000 ）

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  • ゴルジ体の動態解明に基づく糖鎖修飾の制御

    2021 - 2026

    科学技術振興機構  戦略的な研究開発の推進 戦略的創造研究推進事業 CREST 

    加藤 晃一, 甲賀 大輔, 戸島 拓郎, 光山 統泰

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    タンパク質の糖鎖修飾を制御することはバイオ医薬開発における重要課題です。本研究は糖鎖修飾の舞台としてのゴルジ体に着目し、その微細構造の時空間ダイナミクスと糖タンパク質の輸送経路を明らかにします。複雑に区画化されたゴルジ体における糖転移酵素の局在とカーゴ分子の選別輸送の分子機構を探査することにより、分泌経路のプログラムを読み解き、それを改変することでタンパク質の糖鎖修飾を制御することを目指します。

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    J-GLOBAL

  • 負荷で顕在化するホルモン分泌不全の謎：グラニン蛋白欠損マウスから生活習慣病に迫る

    Grant number：20H03411  2020.4 - 2024.3

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    渡部 剛, 甲賀 大輔, 穂坂 正博

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    Grant amount：\17,550,000 （ Direct Cost: \13,500,000 、 Indirect Cost：\4,050,000 ）

    内分泌細胞の分泌顆粒へのペプチドホルモンの選択的な輸送・蓄積過程には、グラニン蛋白と総称される一群の可溶性酸性蛋白の関与が示唆されてきたが、複数のグラニン蛋白の存在による機能的冗長性のために、その生理的意義については未だ不明な点が多い。そこで、本研究課題では、このグラニン蛋白の生理的意義の解明を目指して、2系統の代表的なグラニン蛋白、セクレトグラニンII (Sg2)およびセクレトグラニンIII (Sg3)の遺伝子欠損マウス (KOマウス) を作成し、ホルモン分泌需要を増大させた場合に顕在化する内分泌学的不全症状を解析している。2021年度には、2020年度までに研究分担者の穂坂正博が新規に確立したSg2-KOマウスを高脂肪/高糖質食で飼育し、体重増加曲線の変化や耐糖能異常の有無などを検討した。その結果、既に得られているSg3-KOマウスと同様に、高脂肪/高糖質食で膵島β細胞に負荷をかけると、顕著な肥満や耐糖能の異常など2型糖尿病様の症候が顕在化することが明らかになった。この解析と並行して、やはり代表的なペプチド性内分泌組織である下垂体前葉でグラニン蛋白欠損による影響の有無を明らかにすべく、野生型マウスを比較対象として、Sg2-KO、Sg3-KOマウスの下垂体組織における遺伝子発現の変化をDNAマイクロアレイで比較・解析した。その結果、Sg2-KO、Sg3-KOのどちらのグラニン欠損マウスの下垂体でも、下垂体前葉ホルモン遺伝子の発現が全般的かつ顕著に低下（野生型と比較して50-25％）していることが明らかになった。この実験結果から、グラニン蛋白の欠損は視床下部性の下垂体前葉ホルモン放出ホルモンの分泌障害を誘発するのではないかと考え、視床下部-下垂体系の神経分泌細胞におけるグラニン蛋白の発現様式の詳細な解析に着手した。

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  • Elucidation of the functional significance of primary cilia-Golgi associated zone in endocrine cells

    Grant number：20H04896  2020.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

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    Grant amount：\7,020,000 （ Direct Cost: \5,400,000 、 Indirect Cost：\1,620,000 ）

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  • 内分泌細胞における入出力の会合点・一次線毛-ゴルジ連携ゾーンの機能的意義の解明

    2020 - 2021

    日本学術振興会  新学術領域研究 (研究領域提案型) 

    甲賀 大輔

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    Authorship：Principal investigator 

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  • 先端的イメージング技術で解き明かす下垂体前葉細胞に特異な一次線毛の存在意義

    2019.4 - 2022.3

    基盤研究(C)

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    下垂体前葉内分泌細胞には一次線毛が存在するが、その機能や形態学的詳細は不明なままである。下垂体前葉は、少なくても5種類の内分泌細胞が混在する複雑な組織であるため、各細胞を正確に同定した上で、線毛の機能・構造解析が重要となる。申請者がこれまで開発してきた先端的な走査電子顕微鏡(SEM)・3D解析法は、複雑な細胞構成の組織中で特定の細胞を同定し、その3D微細構造を明らかにすることができる。そこで本研究では、この新たな3D技法を駆使し、下垂体前葉内分泌細胞の一次線毛の形態的多様性、および発生・分化・成長に伴う一次線毛の構築起点を細胞の機能成熟と関連させて明らかにする。さらに、SEMによる革新的な「細胞表面レセプター局在解析法」も新たに確立し、下垂体前葉組織に特異な一次線毛の存在意義を解明する。

  • The elucidation of the significance of unique primary cilia in the anterior pituitary by modern 3D imaging techniques.

    Grant number：19K07261  2019.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

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    Authorship：Principal investigator 

    Grant amount：\4,290,000 （ Direct Cost: \3,300,000 、 Indirect Cost：\990,000 ）

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  • 先端的３Dイメージング技法によるゴルジ装置の新たな形態学的基盤構築

    2019

    公益財団法人 秋山記念生命科学振興財団 (研究助成一般) 

    甲賀 大輔

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    Authorship：Principal investigator  Grant type：Competitive

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  • Ninj1-mediated vascular maturation and development of therapeutic strategy for atherosclerosis

    Grant number：17H04170  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Kawabe Jun-ichi

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    Authorship：Coinvestigator(s) 

    Grant amount：\16,120,000 （ Direct Cost: \12,400,000 、 Indirect Cost：\3,720,000 ）

    Atherosclerosis is a fundamental condition for cardiovascular diseases. It is well recognized that atherosclerotic events are initiated by damage in inner side of vascular walls. We found that in addition to inner side, event in adventitial side, especially abnormality in the formation of microvasculature closely contribute to progression of atherosclerotic plaque. We also found that abnormality of microvascular formation is due to an interaction of PCs and EC tubes and perivascular wiring of peripheral nerve.

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  • Diversity of the Golgi apparatus and maturation process: creation of the Golgi library

    Grant number：16K08458  2016.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Koga Daisuke

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    Authorship：Principal investigator 

    Grant amount：\4,680,000 （ Direct Cost: \3,600,000 、 Indirect Cost：\1,080,000 ）

    In this study, we have visualized the entire three-dimensional (3D) shape of the Golgi apparatus in anterior pituitary endocrine cells and revealed the morphological diversity of this organelle in individual endocrine cells by using a serial-section scanning electron microscopy (SEM) and 3D reconstruction method. Because the anterior pituitary comprises several different hormone-producing cells, it is difficult to identify each endocrine cell type in this tissue by only morphological analysis. Thus, we developed a novel 3D imaging technique that combines serial-section SEM and immunocytochemistry. Using this novel method, we were able to analyze the 3D structure of the Golgi apparatus in targeted anterior pituitary cells that were identified accurately by the immunocytochemical approach.

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  • Morphofunctional analyses of autophagy for understanding the mechanism of the isolation membrane formation

    Grant number：15H04670  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Satoshi Waguri, KOMATSU MASAAKI, USHIKI TATSUO

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    Authorship：Coinvestigator(s) 

    Grant amount：\17,420,000 （ Direct Cost: \13,400,000 、 Indirect Cost：\4,020,000 ）

    This study aimed to identify precursor structures of the autophagic isolation membrane. Because another group demonstrated the ones induced by amino acid starvation using similar approaches, we focused on investigating on mitophagy. As a results, we found that the extension of the isolation membrane on the mitochondrial surface is involved in the iron-deficiency induced mitophagy. Also, we were able to establish a method for observing the isolation membrane by scanning electron microscopy, which will promote future research project. Finally, we suggested the importance of autophagy-lysosomal system in fibroblasts during wound-healing process and in muscle fibers in cancer induced cachexia.

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  • Elucidation of the 3D structure and morphological diversity of the Golgi apparatus by modern 3D imaging technique

    Grant number：26860128  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Koga Daisuke

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    Authorship：Principal investigator 

    Grant amount：\3,380,000 （ Direct Cost: \2,600,000 、 Indirect Cost：\780,000 ）

    The present study developed a novel three-dimensional (3D) imaging technique combining array tomography and osmium impregnation methods. This novel method was applied to analysis of the 3D shape of the entire Golgi apparatus, which exhibits a spatially complex structure. The results clearly demonstrate that complete Golgi apparatus structures are both diverse in shape and cell type-dependent, with each apparatus appearing as a single mass located within a large cytoplasmic region. In addition, morphological changes of the Golgi apparatus were analyzed in gonadotropes before and after castration by correlative light and scanning electron microscopy paired with a combination of immunofluorescence imaging and osmium maceration methods.

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  • 先端的な3Dイメージング法によるゴルジ装置の全体像とその多様性の解明

    2014.4 - 2016.3

    若手研究(B)

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    ゴルジ装置が細胞の機能に果たす重要性に比して、その構造の多様性と複雑性については不明な点も多い。そこで本研究は、先端的な 3D 技法を駆使して、光学顕微鏡レベルと電子顕微鏡レベルを結び付けたゴルジ装置の立体微細構造解析を行う。具体的には、ゴルジ装置の良く発達した下垂体性腺刺激ホルモン産生細胞、膵外分泌細胞、精巣上体管細胞などを用い、超薄連続切片を走査電子顕微鏡を用いて 3D 再構築する新しい手法を確立し、ゴルジ装置の立体像を明らかにする。さらに光顕免疫組織化学と走査電子顕微鏡の相補解析法を用い、ゴルジ装置の複雑な構造の中の極性(シス・トランス)を明らかにし、細胞の機能に関連したゴルジ装置の形状の多様性について明らかにする。

  • Biological application of micro-dissection techniques for scanning electron microscopy

    Grant number：24659081  2012.4 - 2014.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    USHIKI Tatsuo, KOGA Daisuke, NAKAJIMA Masato

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    Authorship：Coinvestigator(s) 

    Grant amount：\3,900,000 （ Direct Cost: \3,000,000 、 Indirect Cost：\900,000 ）

    In the present study, we introduced a microdissection technique which can be used in a stereoscopic scanning electron microscope (3D-SEM) and applied this technique to the study on the three-dimensional ultrastructure of cells and tissues.
    We developed preparation methods suitable for dissecting samples by the two micro-manipulators in the 3D-SEM. We also used the improved manipulators with a needle, micro-scissors and micro-forceps. Using these micro-manipulators, we succeeded in dissecting some tissue samples(e.g, kidney, inner ear, etc) in a 3D-SEM. Thus, the SEM-dissection technique in a 3D-SEM is very useful for the morphological study on cells and tissues.

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  • 凍結切片法を用いた新しい走査電子顕微鏡試料作製法の開発

    2012

    新潟大学塚田医学奨学金 

    甲賀 大輔

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    Authorship：Principal investigator  Grant type：Competitive

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  • Development of scanning probe microscopy in biomedical fields.

    Grant number：21390051  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    USHIKI Tatsuo, HOSHI Osamu, KOGA Daisuke, NAKAJIMA Masato, IWATA Futoshi

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    Authorship：Coinvestigator(s) 

    Grant amount：\18,720,000 （ Direct Cost: \14,400,000 、 Indirect Cost：\4,320,000 ）

    In this project, we studied applicability of the following three techniques of scanning probe microscopy(SPM) to biological fields : 1) Real-time imaging in liquid by atomic force microscopy(AFM) 2) Scanning ion-conductance microscopy(SICM) 3) Manipulation using AFM manipulators Our results showed that these techniques are useful in the field of microscopic anatomy and expected to become powerful tools for imaging and manipulation of cells and tissues.

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  • Three-dimensional structural and functional analysis of the Golgi apparatus by new method of scanning electron microscopy

    Grant number：21790176  2009 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    KOGA Daisuke

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    Authorship：Principal investigator 

    Grant amount：\3,900,000 （ Direct Cost: \3,000,000 、 Indirect Cost：\900,000 ）

    A novel high resolution three-dimensional observation method was developed by an effective combination of light microscopic technique and scanning electron microscopic observation. This method was especially useful for investigation of tissues containing various kinds of cell, enabling the classification of anterior pituitary cells which has hitherto been difficult by using scanning electron microscopy. Furthermore, the three-dimensional ultrastructure in relation to its function of each anterior pituitary cell of the Golgi apparatus was precisely analyzed by high-resolution scanning electron microscopy.

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  • Microscopic studies on the high-order structure of chromosomes in relation to their dynamics during mitosis.

    Grant number：18390058  2006 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    USHIKI Tatsuo, HOSHI Osamu, KOGA Daisuke

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    Authorship：Coinvestigator(s) 

    Grant amount：\16,780,000 （ Direct Cost: \14,200,000 、 Indirect Cost：\2,580,000 ）

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Teaching Experience

  • Laboratory in Osteology

    2015 Institution：Asahikawa Medical College

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  • Histology

    2015 Institution：Asahikawa Medical College

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  • Laboratory in Histology

    2015 Institution：Asahikawa Medical College

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  • Laboratory in Histology

    2008 - 2014 Institution：Niigata University

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  • Histology

    2008 - 2014 Institution：Niigata University

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    2023.8

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    2023.6

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「DNA」  2019.12

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「下垂体」  2019.12

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「鼻」  2019.12

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「すい臓」  2019.12

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    Type：TV or radio program

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  • NHKスペシャル「食の起源」 への画像提供

    Role(s)： Informant

    NHK  第２集 「塩」 人類をとりこにする“本当の理由”  2019.12

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「皮膚」  2019.8

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「口」  2019.8

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「耳」  2019.8

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「目」  2019.8

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    Type：TV or radio program

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  • NHKスペシャル 人体Ⅱ 遺伝子へ画像提供

    Role(s)： Informant

    NHK  第2集 “ＤＮＡスイッチ”が 運命を変える  2019.5

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    Type：TV or radio program

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  • NHKスペシャル 人体Ⅱ 遺伝子へ画像提供

    Role(s)： Informant

    NHK  第1集 あなたの中の宝物“トレジャーDNA”  2019.5

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    Type：TV or radio program

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  • 第1部 「人体」の貴重な映像はどう生まれたか！(45分) 第2部 ミクロの世界の魅力とは？(45分)

    Role(s)： Appearance

    NHK  トークショー NHKスペシャル「人体」からだの不思議を語る！  2019.4

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    Type：Other

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  • NHK Eテレ 「きょうの健康」へ画像提供

    Role(s)： Informant

    NHK Eテレ  「あなたの体を総チェック！②症状別！血液で病気を見つけ出せ」  2019.4

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    Type：TV or radio program

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  • 2019年　医学会総会　名古屋 ＮＨＫスペシャル「人体」からだの不思議を語る！

    2019.4

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    NHKスペシャル「人体」に提供した画像についてや、「ミクロの世界の魅力」について、トークショーというスタイルで子供達や一般の方々に紹介した。

  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「赤ちゃん」  2019.3

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「肝臓」  2019.3

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「血」  2019.3

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    Type：TV or radio program

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  • 市民公開講座 (順天堂大学 本郷・お茶の水キャンパス)

    Role(s)： Lecturer

    「魔法の虫メガネ、走査電子顕微鏡の魅力」の講演  2019.3

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    Type：Lecture

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「小腸」  2018.12

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「肺」  2018.12

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「脂肪」  2018.12

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    Type：TV or radio program

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  • 「電子顕微鏡のまち・米子市」ミクロの探索コーナー開設記念講演会

    Role(s)： Lecturer

    「魔法の虫メガネ 走査電子顕微鏡の魅力～NHKスペシャル人体収録エピソード～」の講演  2018.9

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    Type：Lecture

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  • 「電子顕微鏡のまち・米子市」ミクロの探索コーナー開設記念講演会

    2018.9

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    魔法の虫メガネ　走査電子顕微鏡の魅力～NHKスペシャル人体収録エピソード～、と題して、小学生から大人まで幅広い年代層に、電子顕微鏡の魅力について講演した。

  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「骨」  2018.8

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    Type：TV or radio program

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  • NHK Eテレ (NHKエデュケーショナル) 子供向け医学教育番組 「バビブベボディ」への画像提供

    Role(s)： Informant

    NHK Eテレ  「腎臓」  2018.8

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    Type：TV or radio program

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  • NHKスペシャル「人体」 への画像提供

    Role(s)： Informant

    NHK  NHKスペシャル「人体」第6集「“生命誕生”見えた！母と子 ミクロの会話」  2018.3

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    Type：TV or radio program

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  • 国立科学博物館(上野) 特別展 「人体-神秘への挑戦-」への画像提供

    Role(s)： Informant

    国立科学博物館、NHK  特別展 「人体-神秘への挑戦-」  2018.3 - 2018.6

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    Type：Other

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  • NHKスペシャル「人体」への画像提供

    Role(s)： Informant

    NHK  NHKスペシャル「人体」第4集「万病撃退！“腸”が免疫の鍵だった」  2018.1

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    Type：TV or radio program

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  • NHKスペシャル「人体」 への画像提供

    Role(s)： Informant

    NHK  NHKスペシャル「人体」「“骨”が出す！ 最高の若返り物質」  2018.1

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    Type：TV or radio program

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  • 「ミクロの不思議展2」

    Role(s)： Planner

    旭川医科大学  電子顕微鏡のパネル展  2017.12

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    Type：Other

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  • 「人体精密図鑑」への画像提供と監修

    Role(s)： Informant

    NHK  「人体精密図鑑」  2017.11

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    Type：Internet

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  • 「ミクロの不思議展」

    Role(s)： Planner

    旭川医科大学  電子顕微鏡のパネル展  2017.10

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    Type：Other

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  • 「ミクロの世界を体験しよう」

    Role(s)： Interviewer,　Planner

    旭川医大、旭川市教育委員会、日立ハイテクノロジーズ  子供たちの「電子顕微鏡の体験企画」  2017.10

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    Type：Seminar, workshop

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  • NHKスペシャル「人体」 への画像提供

    Role(s)： Informant

    NHK  NHKスペシャル「人体」第一集「“腎臓”が寿命を決める臓が寿命を決める」  2017.10

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    Type：TV or radio program

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  • NHKスペシャル「人体」 への画像提供

    Role(s)： Informant

    NHK  NHKスペシャル「人体」プロローグ  2017.9

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    Type：TV or radio program

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  • NHKスペシャル「人体」 への画像提供

    Role(s)： Informant

    NHK  NHKスペシャル「人体」第2集「驚きのパワー！“脂肪と筋肉”が命を守る」  2017

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    Type：TV or radio program

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  • サイエンス・リーダーズ・キャンプ

    Role(s)： Lecturer,　Demonstrator

    旭川医科大学  講師・実験実習の担当。基調講演「現代生命科学研究における先端研究技術の原理と応用; (電子顕微鏡の原理・医学生物学応用)」についての講演  2016

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    Type：Lecture

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  • テレビ新潟企画 第7回新潟ジュニアメディカルフォーラム

    Role(s)： Lecturer

    新潟大学  「ミクロの世界をたのしもう」の講演  2014.10

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    Audience： Schoolchildren,　Junior students

    Type：Lecture

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  • 高大連携事業

    Role(s)： Lecturer

    栃木県立大田原女子高等学校  「走査電子顕微鏡で眺めたからだ」の講演  2013.12

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    Type：Visiting lecture

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  • サイエンス・パートナーシップ・プログラム

    Role(s)： Lecturer

    旭川医科大学  「走査電子顕微鏡で眺めてからだ」(旭川東栄高校、旭川南高校)の講演  2012

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    Type：Visiting lecture

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  • 高大連携事業

    Role(s)： Lecturer

    新潟南高校  「走査電子顕微鏡で見た体の世界」の講演  2011.9

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    Type：Visiting lecture

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  • サイエンス・パートナーシップ・プログラム

    Role(s)： Lecturer

    旭川医科大学  「走査電子顕微鏡で眺めてからだ」(旭川東栄高校、旭川南高校)の講演  2011

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    Type：Visiting lecture

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  • サイエンス・パートナーシップ・プログラム

    Role(s)： Lecturer

    旭川医科大学  「走査電子顕微鏡で眺めたからだ」(旭川東栄高校、旭川南高校)の講演  2010.12

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    Audience： High school students

    Type：Visiting lecture

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  • 高大連携事業

    Role(s)： Lecturer

    新潟県立燕中等教育学校  「走査電子顕微鏡で眺めたからだ」の講演  2010.12

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    Type：Visiting lecture

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  • サイエンス・パートナーシップ・プログラム

    Role(s)： Lecturer

    旭川医科大学  「走査電子顕微鏡で眺めたからだ」 (旭川東栄高校、旭川西高校)の講演  2009

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    Type：Visiting lecture

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Media Coverage

  • 「人体の細胞、組織細部まで」 Newspaper, magazine

    北海道新聞  2022.6

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  • 「ミクロの世界 いろんな形があるなあ」 Newspaper, magazine

    朝日新聞  2022.5

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  • 「不死細胞隠れたヒロイン」 Newspaper, magazine

    日本経済新聞  2021.1

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Academic Activities

  • Medical Molecular Morphology

    Role(s)： Peer review

    2025.1

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    Type：Peer review 

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  • 第47回日本分子生物学会年会 シンポジウム「マルチモーダル電顕オルガネラ・イメージング」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2024.11

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    Type：Competition, symposium, etc. 

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  • 日本顕微鏡学会 第67回シンポジウム シンポジウム「S-1 光・電子相関観察法(CLEM)」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2024.11

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    Type：Academic society, research group, etc. 

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  • 第67回顕微鏡学会シンポジウム 実行委員会委員

    Role(s)： Planning, management, etc.

    2024.11

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    Type：Academic society, research group, etc. 

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  • Microscopy

    Role(s)： Peer review

    2024.10

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    Type：Peer review 

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  • Anatomical Science International

    Role(s)： Peer review

    2024.7

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    Type：Peer review 

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  • 第129回日本解剖学会総会・全国学術集会 シンポジウム「電顕オルガネライメージングのシンギュラリティ」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2024.3

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    Type：Academic society, research group, etc. 

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  • 日本顕微鏡学会代議員 International contribution

    2023.4 - 2025.3

  • 第128回日本解剖学会総会・全国学術集会 シンポジウム「オルガネラ・イメージングの電顕マルチモダリティ」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2023.3

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  • 第45回日本分子生物学会年会 フォーラム「電顕によるオルガネライメージングのニューエッジ」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2022.11

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  • 日本顕微鏡学会 第78回学術講演会 医学・生物系プログラム委員 (2022.5.13)

    Role(s)： Planning, management, etc.

    2022.5

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  • オーガナイザー・座長

    2022.4 - 2023.3

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    第128回日本解剖学会総会・全国学術集会　
    「オルガネラ・イメージングの電顕マルチモダリティ」
    シンポジウム企画・座長

  • 第128回日本解剖学会総会・全国学術集会 シンポジウム「電顕技術の深化によるオルガネライメージングの新たな挑戦」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2022.3

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  • 第44回日本分子生物学会年会 ワークショップ「電子顕微鏡イメージング研究の最前線」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2021.12

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    Type：Academic society, research group, etc. 

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  • 第77回日本顕微鏡学会学術講演会 (ハイブリッド開催) シンポジウム「相関顕微鏡法の最前線-高精度かつ大容量の域へ-」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2021.6

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  • 第126回日本解剖学会総会・学術集会/第98回日本生理学会大会合同大会 (オンライン開催) シンポジウム「電子線によるオルガネライメージングの新たな挑戦」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2021.3

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  • 日本顕微鏡学会 第63回シンポジウム 実行委員会委員

    Role(s)： Planning, management, etc.

    2020.11

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  • 第125回日本解剖学会総会・全国学術集会(誌上開催) シンポジウム「先端的電顕技術が切り拓くオルガネライメージングの世界」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2020.3

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  • 第75回日本顕微鏡学会学術講演会 シンポジウム「走査電顕を用いた樹脂包埋切片観察法の基礎と生物試料への応用」 のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2019.6

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    Type：Academic society, research group, etc. 

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  • 日本顕微鏡学会・和文誌「顕微鏡」編集委員

    2019.4

  • Biomedical Research誌編集委員 International contribution

    2019.4

  • 第124回日本解剖学会総会・全国学術集会 “日本解剖学会 若手研究者の集い：「若手研究者の会」設立に向けた意見交換・討論会” の企画・開催

    Role(s)： Planning, management, etc.

    2019.3

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  • 第124回日本解剖学会総会・全国学術集会 シンポジウム「細胞内スーパーイメージングの最先端」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2019.3

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  • 日本解剖学会・若手研究者の会　

    2019.3

  • SCANTECH 2018 実行委員会委員

    Role(s)： Planning, management, etc.

    2018.8

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  • SCANTECH 2017 実行委員会委員

    Role(s)： Planning, management, etc.

    2017.9

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  • 日本顕微鏡学会第73回学術講演会 シンポジウム「徳安法 (凍結超薄切片法)の基礎と応用」のオーガナイザー・座長

    Role(s)： Planning, management, etc.

    2017.5 - 2017.6

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  • 第73回日本顕微鏡学会学術講演会 実行委員会委員 (医学・生物系) (～2017.6.1)

    Role(s)： Planning, management, etc.

    2017.5

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    Type：Academic society, research group, etc. 

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  • SCANTECH 2016 実行委員会委員

    Role(s)： Planning, management, etc.

    2016.9

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  • 日本顕微鏡学会 技術認定委員

    2015.4

  • 日本顕微鏡学会　走査電子顕微鏡分科会　幹事

    2015.4 - 2023.3

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