Updated on 2026/03/13

写真a

 
SAKO Yasuhito
 
Organization
School of Medicine Medical Course Basic Medicine Parasitology
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Degree

  • 博士(医学) ( 2011.3   旭川医科大学 )

  • 博士(獣医学) ( 1999.3   北海道大学 )

Research Interests

  • Parasitology

  • parasitology

  • molecular biology

  • infectious diseases

  • laboratory medicine

Research Areas

  • Life Science / Parasitology

  • Life Science / Infectious disease

  • Environmental Science/Agriculture Science / Environmental impact assessment

Education

  • Hokkaido University   Graduate School, Division of Veterinary Medicine

    - 1999.3

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    Country： Japan

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  • Hokkaido University

    - 1999

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    Country： Japan

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  • Hokkaido University   Graduate School, Division of Veterinary Medicine

    - 1999

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  • Hokkaido University   Faculty of Veterinary Medicine   Veterinary Medicine

    - 1995.3

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    Country： Japan

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  • Hokkaido University   Faculty of Veterinary Medicine

    - 1995

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Professional Memberships

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Papers

  • Comparative assessment of immunochromatographic test kits using low-molecular-weight antigens from cyst fluids of two different genotypes of Taenia solium for serodiagnosis of human cysticercosis. Reviewed International journal

    Lakkhana Sadaow, Penchom Janwan, Patcharaporn Boonroumkaew, Rutchanee Rodpai, Oranuch Sanpool, Tongjit Thanchomnang, Marcello Otake Sato, Pewpan M Intapan, Hiroshi Yamasaki, Yasuhito Sako, Toni Wandra, Kadek Swastika, Wanchai Maleewong

    Parasite (Paris, France)   33   3 - 3   2026

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Human cysticercosis is a serious zoonosis caused by infection with larvae (cysticerci) of the pork tapeworm, Taenia solium. Infection can involve the nervous system, causing chronic headache and intracranial hypertension, focal neurological deficits, epileptic seizures, and paralysis. The disease is found in developing countries, where porcine cysticercosis is prevalent and undercooked pork is habitually consumed. This study aimed to develop immunochromatography-based test (ICT) kits, using low-molecular-weight antigens purified from cyst fluids of Latin American and Asian genotypes of T. solium. To evaluate the kits, we used 164 serum samples, including 24 from proven/confirmed cysticercosis cases, 110 from cases with other parasitoses, and 30 from healthy individuals. Diagnostic performances were calculated. The sensitivity, specificity, and accuracy were 83.3% (95% CI [62.6-95.3]), 93.6% (95% CI [88.1-97.0]), and 92.1% (95% CI [86.8-95.7]), respectively for the American genotype-based ICT kit, while for the Asian genotype-based ICT kit, they were 87.5% (95% CI [67.6-97.3]), 98.6% (95% CI [94.9-99.8]), and 97.0% (95% CI [93.0-99.0]), respectively. The sensitivity and specificity did not significantly differ between the two ICT kits (exact McNemar's test; p > 0.05), with a concordance of 93.9%, represented by a Cohen's kappa of 0.77 (p < 0.001), indicating substantial agreement. These results indicate that affinity-purified antigens from different geographical isolates can be used for the diagnosis of human cysticercosis. The diagnostic specificities were better than for a previously reported ICT kit that used crude antigen.

    DOI： 10.1051/parasite/2026003

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  • Soil-based environmental DNA enables detection of Oncomelania hupensis quadrasi and Schistosoma japonicum microhabitats for schistosomiasis japonica surveillance and control in the Philippines. Reviewed International journal

    Joseph E Valencia, Marcello Otake Sato, Mark June Revolteado, Phoyphaylinh Prasayasith, Mario Jiz, Eleonor A Cervantes, Ralph N Aniceto, Marianette Inobaya, Pengfei Cai, Darren J Gray, Catherine A Gordon, Lydia R Leonardo, Yasuhito Sako, Megumi Sato

    Infectious diseases of poverty   14 ( 1 )   110 - 110   2025.10

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    BACKGROUND: Schistosomiasis japonica, caused by Schistosoma japonicum, remains a significant public health concern in the Philippines, where 12.4 million people are at risk due to persistent transmission in endemic regions. The distribution of schistosomiasis is closely linked to the distribution of its snail intermediate host. This study aims to assess the potential of using soil samples to detect the environmental presence of Oncomelania hupensis quadrasi and Schistosoma japonicum. METHODS: This cross-sectional observational study utilized a soil-based environmental DNA (eDNA) detection system for simultaneous detection of O. h. quadrasi, and S. japonicum mitochondrially encoded cytochrome c oxidase subunit 1 gene in multiplex quantitative real-time PCR and digital PCR platforms. A two-phase sample collection and testing were carried out in December 2023 (Phase 1) and March 2024 (Phase 2) across 30 selected sampling sites in Ekiran village, Leyte, Philippines. Wilcoxon two-sample/Mann-Whitney U test was used to determine whether there was a significant difference in eDNA presence and edaphic factors, while percent agreement was used to assess the concordance among methods. RESULTS: This study reveals that S. japonicum eDNA can be detected in soil samples and confirms the strong applicability of soil-based eDNA for detecting O. h. quadrasi snails and even in sites without visible snail presence. Furthermore, it demonstrates the superiority of soil-eDNA system compared to classical malacological surveys in detecting O. h. quadrasi and S. japonicum microhabitats: in Phase 1, eDNA detected O. h. quadrasi and S. japonicum in 50% (3/6) and 66.67% (4/6) of sites, respectively, while malacological surveys detected them in only 50% and 16.67% (1/6) of sites. In Phase 2, eDNA detected O. h. quadrasi in 20% (6/30) of sites compared to only 10% (3/30) by malacological survey, and S. japonicum was detected only by eDNA in 10% (3/30) of sites. Among the measured soil parameters, only pH showed a statistically significant difference between eDNA-positive and eDNA-negative sites (P = 0.04). CONCLUSIONS: Soil-based eDNA sensitively detected O. h. quadrasi and S. japonicum, enabling scalable, non-invasive transmission site identification and outperforming traditional surveys without visible snails. Its ability to detect S. japonicum highlights its value for comprehensive schistosomiasis monitoring.

    DOI： 10.1186/s40249-025-01374-w

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  • A new focus of Taenia asiatica taeniasis in North Sumatra, Indonesia: Molecular confirmation and prevalence. Reviewed International journal

    Dewi Masyithah Darlan, Hemma Yulfi, Sunna Vyatra Hutagalung, Merina Pangabean, Yunilda Andriyani, Irma Sepala Sari Siregar, Inke Nadia Diniyanti Lubis, Toni Wandra, Ivan Elisabeth Purba, Hiroshi Yamasaki, Yasuhito Sako

    Parasitology international   105   102996 - 102996   2025.4

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    In the Samosir Island of North Sumatra, Indonesia, the prevalence of taeniasis caused by Taenia asiatica was reported as 2.2 %-20.7 % during 1972-2005. In 2014, another T. asiatica-endemic area was confirmed in the Silau Kahean sub-district of Simalungun District, which is geographically distant from Samosir Island. This study was conducted in the Raya Kahean sub-district of Simalungun District adjacent to the Silau Kahean sub-district in September 2023. The aim was to identify a new T. asiatica-endemic area based on molecular confirmation in addition to determining the prevalence of taeniasis. Men aged ≥18 years and palm plantation male farmers showed a higher prevalence of taeniasis, with an overall prevalence of 21.7 % (93/428). A total of 14 proglottids obtained from 14 tapeworm carriers were identified as T. asiatica by COX1-multiplex PCR analysis, and a new T. asiatica-endemic area was detected in the Raya Kahean sub-district. Genetic analyses of COX1 and pold revealed that T. asiatica from North Sumatra has a poor genetic diversity and is a descendant of the hybrids of T. asiatica and T. saginata. Infections were associated with a habitual consumption of raw pig and wild boar livers. Basic sanitary education, improvement of traditional food habits, and implementation of sentinel surveillance and treatment for tapeworm carriers in endemic areas are required to prevent and control this parasitic disease.

    DOI： 10.1016/j.parint.2024.102996

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  • Seasonality and dynamics of schistosomiasis in the environment: usefulness of environmental DNA (eDNA) surveillance system at a community level for risk mapping schistosomiasis in Ekiran Village, Leyte, Philippines. Reviewed International journal

    Mark June Revolteado, Marcello Otake Sato, Joseph Valencia, Mario Jiz, Eleonor Cervantes, Ralph Aniceto, Marianette Inobaya, Darren Gray, Catherine A Gordon, Pengfei Cai, Yasuhito Sako, Megumi Sato

    mSphere   e0106124   2025.3

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    UNLABELLED: Schistosomiasis, primarily caused by Schistosoma japonicum (Sj) in Asia, remains a major health concern in the Philippines, affecting 12.4 million people and causing symptoms like fever, abdominal pain, and hepatosplenomegaly. Chronic disease leads to stunting in children, and reinfection persists despite efforts to reduce morbidity. Current strategies focused on mass drug administration (MDA) and sporadic snail surveys, leaving gaps in monitoring and mitigating schistosomiasis transmission in the environment. To address these issues, this study refined an environmental DNA (eDNA)-based qualitative real-time polymerase chain reaction assay by making it field-applicable and multiplex, to detect both the parasite S. japonicum and its intermediate snail host, Oncomelania hupensis quadrasi (Ohq), using water samples. We surveyed the 30 sentinel sites quarterly-from July 2023 to March 2024-in Ekiran Village, Alangalang, Leyte, Philippines. Collectively, the eDNA of O.h. quadrasi was detected in 18 sites and that of S. japonicum eDNA was detected in 16 sites, while direct snail observation confirmed the presence in only five sites, with infected snails found in only one site. Consequently, the assay described temporal variation of Ohq and Sj, revealing the dynamics of Ohq colonies and Sj in Ekiran's water sources. The eDNA confirmed the focality of Ohq and showed the erratic presence of Sj. Interestingly, both target species' eDNA was observed more during the rainy season (December and March), which suggests a higher infection probability during this period. Integrating eDNA detection system with the existing control programs will enhance the identification of transmission hotspots, which may aid in reducing exposure risk for both humans and animals in the endemic areas. IMPORTANCE: This study aimed to fill the gaps in monitoring and mitigating schistosomiasis transmission in the environment. This field-applicable environmental DNA (eDNA)-based qualitative real-time polymerase chain reaction (qPCR) detection system focused on effectively detecting Schistosoma japonicum and its snail intermediate host, Oncomelania hupensis quadrasi, at the community level, moving from the traditional detection methods that are labor-intensive, less sensitive, and exposing surveyors to potential risk of infection. By introducing a field-applicable eDNA-based qPCR assay, this research provides a sensitive, non-invasive, and rapid molecular method for detecting S. japonicum and O.h. quadrasi in the environment. Additionally, the study not only provided insights in enhanced surveillance strategies but also contributed to a holistic eco-health approach by generating hazard maps for potential transmission and contamination sites, which could improve future control efforts and resource allocation for schistosomiasis elimination.

    DOI： 10.1128/msphere.01061-24

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  • Schistosomiasis in the Philippines: A Comprehensive Review of Epidemiology and Current Control. Reviewed International journal

    Emmanuel John Tabilin, Darren J Gray, Mario A Jiz, Mary Lorraine Mationg, Marianette Inobaya, Eleonor Avenido-Cervantes, Megumi Sato, Marcello Otake Sato, Yasuhito Sako, Yi Mu, Hong You, Matthew Kelly, Pengfei Cai, Catherine A Gordon

    Tropical medicine and infectious disease   10 ( 2 )   2025.1

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    Schistosomiasis japonica is an infectious parasitic disease caused by infection with the blood fluke Schistosoma japonicum, which is endemic in China, small pockets of Indonesia, and the Philippines. Of the three countries, the prevalence of infection is the highest in the Philippines, despite decades of mass drug administration (MDA). As a zoonosis with 46 potential mammalian definitive hosts and a snail intermediate host, the control and eventual elimination of S. japonicum requires management of these animal hosts in addition to new interventions for the human hosts, including health education and water, sanitation, and hygiene (WASH) infrastructure. In this review we examine the status and epidemiology of S. japonicum in the Philippines with an overview of the current control program there and what needs to be accomplished in the future to control and eliminate this disease in the country.

    DOI： 10.3390/tropicalmed10020029

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  • Development of a latex microsphere-based lateral flow immunoassay for the diagnosis of schistosomiasis japonica. Reviewed International journal

    Emmanuel John Tabilin, Catherine A Gordon, Yi Mu, Mario Jiz, Marianette Inobaya, Eleonor Avenido-Cervantes, Darren Gray, Mary Lorraine Mationg, Donald P McManus, Thomas G Egwang, Moses Adriko, Yasuhito Sako, Marcello Otake Sato, Megumi Sato, Hong You, Matthew Kelly, Pengfei Cai

    PLoS neglected tropical diseases   18 ( 12 )   e0012742   2024.12

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    BACKGROUND: Zoonotic schistosomiasis, caused by Schistosoma japonicum, is prevalent in China, the Philippines and Indonesia. Rapid point-of-care (POC) diagnostics are attractive and promising tools for evaluating the efficacy of intervention strategies for schistosomiasis control. METHODOLOGY: The diagnostic potential of five recombinant antigens was tested by enzyme-linked immunosorbent assay (ELISA) using sera from individuals with positive Kato-Katz (KK) results for S. japonicum (n = 28) and non-endemic controls (n = 12). A latex microsphere (LM)-based lateral flow immunoassay (LFIA) incorporating the recombinant SjSAP4 (rSjSAP4) was developed for the diagnosis of schistosomiasis japonica. The test conditions including diluent, dilution factor and reaction time, were optimised for the developed LFIA. Under the optimised conditions, serum samples from individuals living in a barangay endemic for S. japonicum (n = 549) and non-endemic controls (n = 50) were tested with the established LFIA cassettes. The results were imaged by a smartphone and analysed by the ImageJ program. The intensity ratio of the test line to the control line (T/C ratio) was calculated for each cassette. MAIN FINDINGS: ELISA confirmed that rSjSAP4 was the optimal candidate for serological diagnosis of schistosomiasis japonica. Under optimal testing conditions, the developed LFIA strips had a sensitivity of 80.6% and a specificity of 98.0% at a cut-off T/C ratio of 0.1031. Moreover, the results of the LM-based LFIA was positively correlated with those obtained from the rSjSAP4-ELISA (r = 0.8270, 95% CI, 0.7990-0.8514; p < 0.0001). The schistosomiasis prevalence determined by the LFIA strips was about 1.8 times greater than that obtained with the 6-slide KK procedure performed on three stool samples. CONCLUSIONS/SIGNIFICANCE: The developed LFIA represents a POC diagnostic tool that is suitable for onsite screening of human S. japonicum infection with minimal equipment needed. The established immunochromatographic assay complies with most of the WHO's ASSURED criteria for POC diagnostics.

    DOI： 10.1371/journal.pntd.0012742

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  • Molecular identification of Spirometra infections in companion animals and wildlife in Japan. Reviewed

    Hiroshi Yamasaki, Hiromu Sugiyama, Yasuyuki Morishima, Yasuhito Sako

    The Journal of veterinary medical science   86 ( 4 )   409 - 412   2024.4

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    Spirometra infections in companion animals and wildlife in Japan have been diagnosed based on the morphology of the adult worms and eggs, and the etiological agent has been mainly ascribed to Spirometra erinaceieuropaei. However, recent studies have revealed that two other species, Spirometra mansoni and Spirometra asiana, coexist in Japan. Spirometra asiana is a new species recently discovered in Japan. Although morphological discrimination between these two species is difficult, molecular identification is useful. Therefore, to understand which species commonly parasitizes companion animals and wildlife in Japan, a preliminary study was performed based on mitochondrial DNA analysis. Eleven adult worms examined were identified as S. mansoni, suggesting that S. mansoni infects companion animals and wildlife commonly than S. asiana in Japan.

    DOI： 10.1292/jvms.23-0475

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  • Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis. Reviewed International journal

    Lakkhana Sadaow, Patcharaporn Boonroumkaew, Rutchanee Rodpai, Penchom Janwan, Oranuch Sanpool, Tongjit Thanchomnang, Yasuyuki Morishima, Marcello Otake Sato, Yasuhito Sako, Kaoru Kobayashi, Misako Iwai, Wanchai Maleewong, Hiroshi Yamasaki, Pewpan M Intapan

    Food and waterborne parasitology   33   e00211   2023.12

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    Human cysticercosis is a life-threatening zoonotic disease caused by infection with larvae (cysticerci) of the pork tapeworm, Taenia solium. This can affect the nervous system causing chronic headache and intracranial hypertension, potentially leading to epileptic seizures and paralysis. The disease is found in developing countries, especially in Southeast and South Asia, Sub-Saharan Africa, and Central and South America where porcine cysticercosis is endemic and people have a habit of eating undercooked pork. An immunochromatography-based test (ICT) kit, using T. solium cyst fluid as antigen, was manufactured to detect anti-T. solium IgG antibodies in human serum. To evaluate the kit, we used 187 serum samples including 24 from proven/confirmed cysticercosis cases, 133 from cases with other parasitosis and 30 healthy controls. Diagnostic efficiencies were calculated. The sensitivity, specificity, and accuracy were 83.3%, 92.0%, and 90.9%, respectively. Moreover, the ICT was positive before treatment but became negative after treatment, implying that this kit is also useful for follow-up monitoring post-treatment. In conclusion, we have successfully developed and present preliminary evaluation of an easy-to-handle rapid diagnostic tool for human cysticercosis in the form of an ICT platform using as antigen fluid from T. solium cysticerci.

    DOI： 10.1016/j.fawpar.2023.e00211

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  • Dibothriocephalus nihonkaiensis incidentally detected at colonoscopy. Reviewed

    Kubo K., Kato M., Sako Y.

    Clin Gastroenterol Hepatol   2022.1

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  • Origin of the pork tapeworm Taenia solium in Bali and Papua, Indonesia. Reviewed International journal

    Tetsuya Yanagida, Kadek Swastika, Nyoman Sadra Dharmawan, Yasuhito Sako, Toni Wandra, Akira Ito, Munehiro Okamoto

    Parasitology international   83   102285 - 102285   2021.8

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    Global distributions of zoonotic pathogens have been strongly affected by the history of human dispersal and domestication of livestock. The pork tapeworm Taenia solium is distributed worldwide as the cause of neurocysticercosis, one of the most serious neglected tropical diseases. T. solium has been reported in Indonesia but only endemic to restricted areas such as Bali and Papua. Previous studies indicated the distinctiveness of a mitochondrial haplotype confirmed in Papua, but only one isolate has been examined to date. In this study, genetic characterization of T. solium and pigs in Bali and Papua was conducted to clarify the distributional history of the parasite. Mitochondrial haplotype network analysis clearly showed that Indonesian T. solium comprises a unique haplogroup which was the first to diverge among Asian genotypes, indicating its single origin and the fact that it was not introduced in the recent past from other area in Asia in which it is endemic. Although phylogenetic analysis based on the mitochondrial D-loop revealed multiple origins of pigs in Bali and Papua, the majority of pigs belonged to the Pacific Clade, which is widely dispersed throughout the Island Southeast Asia (ISEA) and Oceania due to Neolithic human dispersal. Given the results of our network analysis, it is likely that the Pacific Clade pigs played a key role in the dispersal of T. solium. The data suggest that T. solium was introduced from mainland Asia into Western Indonesia, including Bali, by modern humans in the late Pleistocene, or in the early to middle Holocene along with the Pacific Clade pigs. Introduction into New Guinea most likely occurred in the late Holocene through the spread of Pacific Clade pigs. Over time, T. solium has been eradicated from most of Indonesia through the middle to modern ages owing to religious and cultural practices.

    DOI： 10.1016/j.parint.2021.102285

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  • 日本海裂頭条虫感染の1症例報告

    工藤裕基, 畠山真奈美, 仲山春恵, 迫康仁

    臨床検査学教育   11 ( 1 )   121 - 122   2019

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  • Taenia solium, Taenia saginata, Taenia asiatica, their hybrids and other helminthic infections occurring in a neglected tropical diseases' highly endemic area in Lao PDR Reviewed

    Marcello Otake Sato, Megumi Sato, Tetsuya Yanagida, Jitra Waikagul, Tiengkham Pongvongsa, Yasuhito Sako, Surapol Sanguankiat, Tipparayat Yoonuan, Sengchanh Kounnavang, Satoru Kawai, Akira Ito, Munehiro Okamoto, Kazuhiko Moji

    PLoS Neglected Tropical Diseases   12 ( 2 )   2018.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science  

    DOI： 10.1371/journal.pntd.0006260

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  • Genetic and morphological characterization of Thysaniezia tapeworms from cattle and sheep in Senegal. Reviewed International journal

    Mallé Ndom, Tetsuya Yanagida, Gora Diop, Yann Quilichini, Aïssatou Ba, Yasuhito Sako, Minoru Nakao, Bernard Marchand, Alioune Dieye, Cheikh Tidiane Ba, Akira Ito

    Veterinary parasitology, regional studies and reports   11   27 - 31   2018.1

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    Genetic and morphological diversity of Thysaniezia tapeworms from cattle and sheep in Senegal was investigated using light and scanning microscopic observations and molecular analysis based on mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear small subunit ribosomal RNA gene (SSU rDNA). A total of 52 adult tapeworms were collected from sheep and cattle. Although the tapeworms of the two hosts were morphologically very close, phylogenetic analysis based on cox1 and SSU rDNA gene sequences showed that they were divided into two clades corresponding each to a host. The maximum pairwise divergence between the clades were 12.1% in cox1 and 2.9% in SSU rDNA, indicating they are distinct species. The tapeworms collected from sheep were morphologically identified as Thysaniezia ovilla, a cosmopolitan species in domestic ruminants. Detailed morphological observations revealed a consistent difference between the tapeworms obtained from sheep and those from cattle. The latter were identified as Thysaniezia connochaeti. The present study highlights presence of two species of Thysaniezia among domestic ruminants in Senegal: T. ovilla specific to sheep and T. connochaeti specific to cattle. Our work is the first report of T. connochaeti from domestic animals.

    DOI： 10.1016/j.vprsr.2017.11.008

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  • Co-occurrence of swine cysticercosis due to Taenia solium and Taenia hydatigena in ethnic minority villages at the Thai-Myanmar border Reviewed

    Chaisiri K., Kusolsuk T., Homsuwan N., Sanguankiat S., Dekumyoy P., Peunpipoom G., Khiriphattharaphon S., Sako Y., Yanagida T., Okamoto M., Ito A.

    Journal of Helminthology   1 - 9   2018

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  • Gastrointestinal helminths and Taenia spp. in parenteral tissues of free-roaming pigs (Sus scrofa indicus) from hill-tribe village at the western border of Thailand Reviewed

    K. Chaisiri, S. Aueawiboonsri, T. Kusolsuk, P. Dekumyoy, S. Sanguankiat, N. Homsuwan, G. Peunpipoom, M. Okamoto, T. Yanagida, Y. Sako, A. Ito

    TROPICAL BIOMEDICINE   34 ( 2 )   464 - 470   2017.6

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  • Seroprevalence and risk factors of human cysticercosis and taeniasis prevalence in a highly endemic area of epilepsy in Bangoua, west Cameroon Reviewed

    Agathe Nkouawa, Armel Romeo Dschanou, Roger Moyou-Somo, Yasuhito Sako, Akira Ito

    ACTA TROPICA   165   116 - 120   2017.1

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    DOI： 10.1016/j.actatropica.2015.12.019

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  • The putative serine protease inhibitor (serpin) genes encoded on Echinococcus multilocularis genome and their expressions in metacestodal stage Reviewed

    Mizuki Sasaki, Yasuhito Sako

    VETERINARY PARASITOLOGY   233   20 - 24   2017.1

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    DOI： 10.1016/j.vetpar.2016.11.019

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  • Serological validation of an alveolar echinococcosis rat model with a single hepatic lesion Reviewed

    Yamashita M., Imagawa T., Sako Y., Okamoto M., Yanagida T., Okamoto Y., Tsuka T., Osaki T., Ito A.

    Journal of Veterinary Medicine and Science   79   308 - 313   2017

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1292/jvms.16-0513

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  • Swine cysticercosis in the Karangasem district of Bali, Indonesia: An evaluation of serological screening methods Reviewed

    Kadek Swastika, Nyoman Sadra Dharmawan, I. Ketut Suardita, I. Nengah Kepeng, Toni Wandra, Yasuhito Sako, Munehiro Okamoto, Tetsuya Yanagida, Mizuki Sasaki, Patrick Giraudoux, Minoru Nakao, Takahiko Yoshida, Luh Putu Eka Diarthini, I. Made Sudarmaja, Ivan Elisabeth Purba, Christine M. Budke, Akira Ito

    ACTA TROPICA   163   46 - 53   2016.11

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    DOI： 10.1016/j.actatropica.2016.07.022

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  • Simple Identification of Human Taenia Species by Multiplex Loop-Mediated Isothermal Amplification in Combination with Dot Enzyme-Linked Immunosorbent Assay Reviewed

    Agathe Nkouawa, Yasuhito Sako, Munehiro Okamoto, Akira Ito

    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE   94 ( 6 )   1318 - 1323   2016.6

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    DOI： 10.4269/ajtmh.15-0829

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  • Genetic characterization of Moniezia species in Senegal and Ethiopia. Reviewed International journal

    Gora Diop, Tetsuya Yanagida, Zerihun Hailemariam, Sissay Menkir, Minoru Nakao, Yasuhito Sako, Cheikh Tidiane Ba, Akira Ito

    Parasitology international   64 ( 5 )   256 - 60   2015.10

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    Genetic diversity of Moniezia spp. from domestic ruminants in Senegal and Ethiopia was investigated based on the nucleotide sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear small subunit ribosomal RNA gene (SSU rDNA). A total of 64 adult tapeworms were collected from sheep, goat and cattle, and the tapeworms from cattle were all morphologically identified as Moniezia benedeni. On the other hand, the tapeworms obtained from sheep and goat were identified as Moniezia expansa or could not be identified because of the lack of diagnostic morphologic character, i.e. interproglottidal glands (IPGs). Phylogenetic analysis based on cox1 gene sequences revealed that the worms from sheep/goat and cattle formed distinct clades, and three mitochondrial lineages were confirmed within the sheep/goat tapeworms. The maximum pairwise divergences among the three mitochondrial linages were about 3% in cox1 and 0.1% in SSU rDNA, while that between the worms from sheep/goat and cattle reached 13% in cox1 and 2.7% in SSU rDNA. All of the three mitochondrial lineages contained tapeworms morphologically identified as M. expansa, and the tapeworms without IPGs were confirmed in one of the three lineages, indicating the tapeworms without IPGs were also M. expansa.

    DOI： 10.1016/j.parint.2015.02.008

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  • Comparative Study of Paired Serum and Cerebrospinal Fluid Samples from Neurocysticercosis Patients for the Detection of Specific Antibody to Taenia solium Immunodiagnostic Antigen. Reviewed

    Yasuhito Sako, Osvaldo M Takayanagui, Newton S Odashima, Akira Ito

    Tropical medicine and health   43 ( 3 )   171 - 6   2015.9

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    Neurocysticercosis (NCC) is an important disease of the central nervous system caused by infection with Taenia solium metacestodes. In addition to the clinical findings and the imaging analysis, the results of immunological tests are informative for the diagnosis of NCC. To compare the usefulness of serum and cerebrospinal fluid (CSF) samples for antibody detection, paired serum and CSF samples from patients with NCC and other neurological diseases were examined by an enzyme-linked immunosorbent assay with low-molecular-weight antigens purified from T. solium cyst fluid in a blinded fashion. The sensitivity of both serum and CSF samples was 25.0% in inactive NCC cases (n = 4) and 90.9% in active NCC cases (n = 33), and the specificity of serum and CSF was 100% and 95.8%, respectively. When the serum and CSF samples were combined, the sensitivity in active NCC cases became 100%. There was no difference in test performance between serum and CSF samples. Based on these results, we recommend the detection of specific antibodies in serum for the diagnosis of active NCC because of the ease of collection. When the antibody test is negative, however, CSF should be used to confirm NCC and to rule out other medical disorders of the central nervous system. Antibody detection test using only serum or CSF has a limited diagnostic value and cannot be recommended for the diagnosis of suspected inactive NCC cases.

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  • The present situation and towards the prevention and control of neurocysticercosis on the tropical island, Bali, Indonesia. Reviewed International journal

    Toni Wandra, Kadek Swastika, Nyoman S Dharmawan, Ivan Elisabeth Purba, I Made Sudarmaja, Takahiko Yoshida, Yasuhito Sako, Munehiro Okamoto, Ni Luh Putu Eka Diarthini, Dewa Ayu Agus Sri Laksemi, Tetsuya Yanagida, Minoru Nakao, Akira Ito

    Parasites & vectors   8   148 - 148   2015.3

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    Neurocysticercosis (NCC), which is caused by accidental ingestion of eggs of the pork tapeworm, Taenia solium, was common in Bali, Indonesia until the early 1990s. However, improved education on hygiene and sanitation, a move to keeping pigs indoors, and improvement of economic and living conditions have substantially reduced the occurrence of NCC in Bali. Since 2011, T. solium tapeworm carriers (T. solium taeniasis) and heavily infected pigs and dogs have exclusively been detected from villages in mountainous regions of northeastern Bali where NCC and ocular cysticercosis (OCC) cases have also been identified. In response to this continued area of high infection, a one-day workshop was convened to discuss how to prevent and control this potentially lethal zoonotic parasitic infection in Bali. This review presents an overview of the current status of T. solium taeniasis and cysticercosis in Indonesia and proposes a strategy for the prevention and control of this zoonosis in Bali.

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  • Evaluation of a New Immunochromatographic Test Using Recombinant Antigen B8/1 for Diagnosis of Cystic Echinococcosis. Reviewed

    Santivanez SJ, Rodriguez ML, Rodriguez S., Sako Y., Nkouawa A., Kobayashi Y., Sotomayor AL, Peralta JE, Valcarcel M., Gonzalez AE, Garcia HH, Ito A.

    Journal of Clinical Microbiology   2015

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    DOI： 10.1128/JCM.02157-15

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  • Cystic Echinococcoses in Mongolia: Molecular Identification, Serology and Risk Factors Reviewed

    Akira Ito, Temuulen Dorjsuren, Anu Davaasuren, Tetsuya Yanagida, Yasuhito Sako, Kazuhiro Nakaya, Minoru Nakao, Oyun-Erdene Bat-Ochir, Tsendjav Ayushkhuu, Narantuya Bazarragchaa, Nyamkhuu Gonchigsengee, Tiaoying Li, Gurbadam Agvaandaram, Abmed Davaajav, Chinchuluun Boldbaatar, Gantigmaa Chuluunbaatar

    PLOS NEGLECTED TROPICAL DISEASES   8 ( 6 )   2014.6

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    DOI： 10.1371/journal.pntd.0002937

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  • Recent situation of taeniasis in Mongolia (2002-2012). Reviewed International journal

    Anu Davaasuren, Temuulen Dorjsuren, Tetsuya Yanagida, Yasuhito Sako, Kazuhiro Nakaya, Abmed Davaajav, Gurbadam Agvaandaram, Tsatsral Enkhbat, Battsetseg Gonchigoo, Nyamkhuu Dulmaa, Gantigmaa Chuluunbaatar, Akira Ito

    The Korean journal of parasitology   52 ( 2 )   211 - 4   2014.4

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    Epidemiological situation of taeniasis in Mongolia was assessed based on mitochondrial DNA identification of the parasite species. Multiplex PCR was used on a total of 194 proglottid specimens of Taenia species and copro-PCR and loop-mediated isothermal amplification (LAMP) assays were utilized for detection of copro-DNA of 37 fecal samples from taeniasis patients submitted to the Mongolian National Center for Communicable Diseases (NCCD) from 2002 to 2012. In addition, 4 out of 44 calcified cysts in beef kept in formalin since 2003 were evaluated for histopathological confirmation of cattle cysticercosis. All proglottid specimens and stool samples were confirmed to be Taenia saginata by multiplex PCR and by copro-PCR and LAMP, respectively. Cysts collected from cattle were morphologically confirmed to be metacestodes of Taenia species. T. saginata taeniasis was identified from almost all ages from a 2-year-old boy up to a 88-year-old woman and most prominently in 15-29 age group (37%, 74/198) followed by 30-44 age group (34.8%, 69/198 ) from 15 of Mongolia's 21 provinces, while cattle cysticerci were found from 12 provinces. The highest proportion of taeniasis patients was in Ulaanbaatar, the capital of Mongolia.

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  • A survey of seropositivity to antigen B, an immunodiagnostic antigen for human cystic echinococcosis, in domestic animals in Mongolia. Reviewed

    Chinchuluun B., Sako Y., Khatanbaatar I., Bayarmaa B., Lkhagvatseren S., Battsetseg G., Yanagida T., Itoh S., Temuulen D., Budke CM, Ito A., Batsukh Z.

    Parasitol Int   63 ( 2 )   324 - 328   2014

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  • Genetics of the pig tapeworm in madagascar reveal a history of human dispersal and colonization. Reviewed International journal

    Tetsuya Yanagida, Jean-François Carod, Yasuhito Sako, Minoru Nakao, Eric P Hoberg, Akira Ito

    PloS one   9 ( 10 )   e109002   2014

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    An intricate history of human dispersal and geographic colonization has strongly affected the distribution of human pathogens. The pig tapeworm Taenia solium occurs throughout the world as the causative agent of cysticercosis, one of the most serious neglected tropical diseases. Discrete genetic lineages of T. solium in Asia and Africa/Latin America are geographically disjunct; only in Madagascar are they sympatric. Linguistic, archaeological and genetic evidence has indicated that the people in Madagascar have mixed ancestry from Island Southeast Asia and East Africa. Hence, anthropogenic introduction of the tapeworm from Southeast Asia and Africa had been postulated. This study shows that the major mitochondrial haplotype of T. solium in Madagascar is closely related to those from the Indian Subcontinent. Parasitological evidence presented here, and human genetics previously reported, support the hypothesis of an Indian influence on Malagasy culture coinciding with periods of early human migration onto the island. We also found evidence of nuclear-mitochondrial discordance in single tapeworms, indicating unexpected cross-fertilization between the two lineages of T. solium. Analyses of genetic and geographic populations of T. solium in Madagascar will shed light on apparently rapid evolution of this organism driven by recent (<2,000 yr) human migrations, following tens of thousands of years of geographic isolation.

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  • Comparison of the serological tests ICT and ELISA for the diagnosis of alveolar echinococcosis in France. Reviewed International journal

    Jenny Knapp, Yasuhito Sako, Frédéric Grenouillet, Solange Bresson-Hadni, Carine Richou, Houssein Gbaguidi-Haore, Akira Ito, Laurence Millon

    Parasite (Paris, France)   21   34 - 34   2014

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    Serological diagnosis of alveolar echinococcosis (AE) is a key element for efficient patient treatment management. A rapid immunochromatography test kit (ICT) using the recombinant Em18 antigen (rEm18) was recently developed. The aim of our study was to assess this test on a panel of sera from French patients with alveolar echinococcosis and control patients. In a blind test, a total of 112 serum samples were tested including samples of AE (n = 30), cystic echinococcosis [CE] (n = 15), and polycystic echinococcosis [PE] (n = 1). For the comparison, 66 sera from patients with hepatocarcinoma, fascioliasis, toxocariasis, Caroli's disease, or autoimmune chronic active hepatitis were used. The diagnostic test sets we used were the rEm18-ICT and two validated ELISAs with rEm18 and Em2-Em18 antigens, respectively. For the ICT, 27/30 sera from AE patients, 4/15 sera from CE patients and the PE patient serum were positive. One serum from the control panel (toxocariasis) was positive for the ICT. The rEm18-ICT sensitivity (90.0%) and specificity (92.7%) for detection of Em18-specific antibodies confirmed it as a relevant tool for AE diagnosis. The rEm18-ELISA had a sensitivity of 86.7% and specificity of 91.5%, and the Em2-Em18-ELISA had a sensitivity of 96.7% and specificity of 87.8%. However, when AE patient sera are recorded as weak in intensity with the ICT, we recommend a double reading and use of a reference sample if the ICT is used for patient follow-up.

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  • Immunodiagnosis of alveolar echinococcosis using urine samples Reviewed

    Makoto Itoh, Yasuhito Sako, Sonoyo Itoh, Yuji Ishikawa, Hiromitsu Akabane, Kazuhiro Nakaya, Fumiaki Nagaoka, Akira Ito

    PARASITOLOGY INTERNATIONAL   62 ( 6 )   514 - 516   2013.12

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    DOI： 10.1016/j.parint.2013.07.007

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  • Detection of human taeniases in Tibetan endemic areas, China Reviewed

    Tiaoying Li, Xingwang Chen, Tetsuya Yanagida, Hao Wang, Changping Long, Yasuhito Sako, Munehiro Okamoto, Yunfei Wu, Patrick Giraudoux, Francis Raoul, Agathe Nkouawa, Minoru Nakao, Philip S. Craig, Akira Ito

    PARASITOLOGY   140 ( 13 )   1602 - 1607   2013.11

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  • Echinococcus species from red foxes, corsac foxes, and wolves in Mongolia Reviewed

    Akira Ito, Gantigmaa Chuluunbaatar, Tetsuya Yanagida, Anu Davaasuren, Battulga Sumiya, Mitsuhiko Asakawa, Toshiaki Ki, Kazuhiro Nakaya, Abmed Davaajav, Temuulen Dorjsuren, Minoru Nakao, Yasuhito Sako

    PARASITOLOGY   140 ( 13 )   1648 - 1654   2013.11

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    DOI： 10.1017/S0031182013001030

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  • Genotypic relationships between taenia saginata, taenia asiatica and their hybrids Reviewed

    Kanako Yamane, Tetsuya Yanagida, Tiaoying Li, Xingwang Chen, Paron Dekumyoy, Jitra Waikagul, Agathe Nkouawa, Minoru Nakao, Yasuhito Sako, Akira Ito, Hiroshi Sato, Munehiro Okamoto

    Parasitology   140 ( 13 )   1595 - 1601   2013.11

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  • Simple and reliable preparation of immunodiagnostic antigens for taenia solium cysticercosis Reviewed

    Y. Sako, S. Itoh, M. Okamoto, K. Nakaya, A. Ito

    Parasitology   140 ( 13 )   1589 - 1594   2013.11

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  • Genetic diversity of Echinococcus spp. in Russia Reviewed

    Sergey V. Konyaev, Tetsuya Yanagida, Minoru Nakao, Galina M. Ingovatova, Yakov N. Shoykhet, Alexandr Y. Bondarev, Valeriy A. Odnokurtsev, Kyunnyay S. Loskutova, Gulnur I. Lukmanova, Nikolai E. Dokuchaev, Sergey Spiridonov, Mikhail V. Alshinecky, Tatyana N. Sivkova, Oleg N. Andreyanov, Sergey A. Abramov, Anton V. Krivopalov, Sergey V. Karpenko, Natalia V. Lopatina, Tamara A. Dupal, Yasuhito Sako, Akira Ito

    PARASITOLOGY   140 ( 13 )   1637 - 1647   2013.11

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  • Serological and Molecular Characteristics of the First Korean Case of Echinococcus multilocularis Reviewed

    Jin-Sook Jeong, Sang-Young Han, Young-Hoon Kim, Yasuhito Sako, Tetsuya Yanagida, Akira Ito, Jong-Yil Chai

    KOREAN JOURNAL OF PARASITOLOGY   51 ( 5 )   595 - 597   2013.10

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  • Echinococcus multilocularis: Single hepatic lesion experimentally established without metastasis in rats Reviewed

    Masamichi Yamashita, Tomohiro Imagawa, Kazuhiro Nakaya, Yasuhito Sako, Yoshiharu Okamoto, Takeshi Tsuka, Tomohiro Osaki, Munehiro Okamoto, Akira Ito

    EXPERIMENTAL PARASITOLOGY   135 ( 2 )   320 - 324   2013.10

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    DOI： 10.1016/j.exppara.2013.07.015

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  • Case Report: Rare Case of Disseminated Cysticercosis and Taeniasis in a Japanese Traveler after Returning from India Reviewed

    Ken-ichiro Kobayashi, Fukumi Nakamura-Uchiyama, Takeshi Nishiguchi, Kenichi Isoda, Yasumasa Kokubo, Katsuhiko Ando, Masaki Katurahara, Yasuhito Sako, Tetsuya Yanagida, Akira Ito, Sentaro Iwabuchi, Kenji Ohnishi

    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE   89 ( 1 )   58 - 62   2013.7

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  • Lethal cestode zoonoses, cysticercosis and echinococcoses in Asia and the World

    A. Ito, Y. Sako, T. Yanagida, M. Nakao, M. Okamoto

    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS   42   S4 - S4   2013.6

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  • Mini review on chemotherapy of taeniasis and cysticercosis due to Taenia solium in Asia, and a case report with 20 tapeworms in China. Reviewed

    Ito A., Li T., Chen X., Long C., Yanagida T., Nakao M., Sako Y., Okamoto M., Wu Y., Raoul F., Giraudoux P., Craig PS

    Trop Biomed   30 ( 2 )   164 - 173   2013

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  • Loop-mediated isothermal amplification method for a differential identification of human taenia tapeworms Reviewed

    Yasuhito Sako, Agathe Nkouawa, Tetsuya Yanagida, Akira Ito

    Methods in Molecular Biology   1039   109 - 120   2013

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    DOI： 10.1007/978-1-62703-535-4_9

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  • Taeniases and cysticercosis in Indonesia: past and present situations. Reviewed

    Wandra T., Ito A., Swastika K., Dharmawan NS, Sako Y., Okamoto M.

    Parasitology   140 ( 13 )   1608 - 1616   2013

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  • Advances in diagnosis and spatial analysis of cysticercosis and taeniasis. Reviewed

    Raoul F., Li T., Sako Y., Chen X., Long C., Yanagida T., Wu Y., Nakao M., Okamoto M., Craig PS, Giraudoux P., Ito A.

    Parasitology   140 ( 13 )   1578 - 1588   2013

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  • Recombinant AgB8/1 ELISA test vs. commercially available IgG ELISA test in the diagnosis of cystic echinococcosis. Reviewed

    Tamarozzi F., Sako Y., Ito A., Piccoli L., Grisolia A., Itoh S., Gatti S., Meroni V., Genco F., Brunetti E.

    Parasite Immunol   2013

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  • A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: Application to a field survey Reviewed

    Agathe Nkouawa, Yasuhito Sako, Tiaoying Li, Xingwang Chen, Minoru Nakao, Tetsuya Yanagida, Munehiro Okamoto, Patrick Giraudoux, Francis Raoul, Kazuhiro Nakaya, Ning Xiao, Jiamin Qiu, Dongchuan Qiu, Philip S. Craig, Akira Ito

    PARASITOLOGY INTERNATIONAL   61 ( 4 )   723 - 725   2012.12

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    DOI： 10.1016/j.parint.2012.06.001

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  • Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East Reviewed

    Tetsuya Yanagida, Tahereh Mohammadzadeh, Shaden Kamhawi, Minoru Nakao, Seyed Mahmoud Sadjjadi, Nawal Hijjawi, Sami K. Abdel-Hafez, Yasuhito Sako, Munehiro Okamoto, Akira Ito

    PARASITOLOGY INTERNATIONAL   61 ( 4 )   599 - 603   2012.12

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    DOI： 10.1016/j.parint.2012.05.014

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  • Molecular identification of human echinococcosis in the Altai region of Russia Reviewed

    Sergey V. Konyaev, Tetsuya Yanagida, Galina M. Ingovatova, Yakov N. Shoikhet, Minoru Nakao, Yasuhito Sako, Alexandr Y. Bondarev, Akira Ito

    PARASITOLOGY INTERNATIONAL   61 ( 4 )   711 - 714   2012.12

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    DOI： 10.1016/j.parint.2012.05.009

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  • The first report on cystic echinococcosis in a cat caused by Echinococcus granulosus sensu stricto (G1) Reviewed

    S. V. Konyaev, T. Yanagida, M. V. Ivanov, V. V. Ruppel, Y. Sako, M. Nakao, A. Ito

    JOURNAL OF HELMINTHOLOGY   86 ( 4 )   391 - 394   2012.12

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  • Usefulness of pumpkin seeds combined with areca nut extract in community-based treatment of human taeniasis in northwest Sichuan Province, China. Reviewed

    Li T., Ito A., Chen X., Long C., Okamoto M., Raoul F., Giraudoux P., Yanagida T., Nakao M., Sako Y., Xiao N., Craig PS

    Acta Trop   124 ( 2 )   152 - 157   2012.10

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  • Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Reviewed

    Mohammadzadeh T., Sako Y., Sadjjadi SM, Sarkari B., Ito A.

    Trans R Soc Trop Med Hyg   106   371 - 375   2012.6

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    DOI： 10.1016/j.trstmh.2012.01.012

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  • An ocular cysticercosis in Bali, Indonesia caused by Taenia solium Asian genotype. Reviewed

    Swastika K., Dewiyani CI, Yanagida T., Sako Y., Sudarmaja M., Sutisna P., Wandra T., Dharmawan NS, Nakaya K., Okamoto M., Ito A.

    Parasitol Int   61 ( 2 )   378 - 380   2012.6

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  • Recent hybridization between Taenia asiatica and Taenia saginata Reviewed

    Kanako Yamane, Yumi Suzuki, Eiko Tachi, Tiaoying Li, Xingwang Chen, Minoru Nakao, Agathe Nkouawa, Testuya Yanagida, Yasuhito Sako, Akira Ito, Hiroshi Sato, Munehiro Okamoto

    PARASITOLOGY INTERNATIONAL   61 ( 2 )   351 - 355   2012.6

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  • Taeniasis and cysticercosis due to Taenia solium in Japan. Reviewed International journal

    Tetsuya Yanagida, Yasuhito Sako, Minoru Nakao, Kazuhiro Nakaya, Akira Ito

    Parasites & vectors   5   18 - 18   2012.1

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    Taenia solium is a zoonotic cestode that causes taeniasis and cysticercosis in humans. The parasite is traditionally found in developing countries where undercooked pork is consumed under poor sanitary conditions and/or as part of traditional food cultures. However, the recent increase in international tourism and immigration is spreading the disease into non-endemic developed countries such as the United States. Although there has been concern that the number of cysticercosis cases is increasing in Japan, the current situation is not clear. This is largely because taeniasis and cysticercosis are not notifiable conditions in Japan and because there have been no comprehensive reviews of T. solium infections in Japan conducted in the last 15 years. Herein, we provide an overview of the status of T. solium infection in Japan over the past 35 years and point out the potential risks to Japanese society.

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  • Echinococcus and Taenia spp. from captive mammals in the United Kingdom. Reviewed

    Boufana B., Stidworthy MF, Bell S., Chantrey J., Masters N., Unwin S., Wood R., Lawrence RP, Potter A., McGarry J., Redrobe S., Killick R., Foster AP, Mitchell S., Greenwood AG, Sako Y., Nakao M., Ito A., Wyatt K., Lord B., Craig PS

    Vet Parasitol   190 ( 1-2 )   95 - 103   2012

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  • Serological and molecular tools to detect neurologic parasitic zoonoses in rural Cameroon. Reviewed International journal

    Agathe Nkouawa, Yasuhito Sako, Roger Moyou-Somo, Akira Ito

    The Southeast Asian journal of tropical medicine and public health   42 ( 6 )   1365 - 74   2011.11

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    Parasitic helminthiases, such as toxocariasis, cysticercosis and paragonimiasis are a public health threat, since they can affect the brain leading to neurological disorders. Epilepsy and paragonimiasis are common in southwestern Cameroon. We reviewed the literature for studies using antigens to diagnose toxocariasis, cysticercosis, and paragonimiasis. Serology revealed that 61 (36.3%), 26 (15.5%) and 2 (1.2%) of 168 persons examined [78 males (15.2 +/- 8.2 years old), 90 females (12.9 +/- 5.9 years old), 143 persons < 20 years old] had antibody responses to toxocariasis, paragonimiasis and cysticercosis, respectively. Of the 14 people with epilepsy, 5 were seropositive for Toxocara antigens and 1 was positive for both Toxocara and Paragonimus antigens. Two children were serologically confirmed to have cysticercosis. Serologic screening for cysticercosis may be feasible to detect asymptomatic cysticercosis in children in endemic areas leading to early treatment. The causative Paragonimus species was confirmed to be P. africanus by molecular sequencing. Education, screening and confirmation test for these diseases may be needed for control in Cameroon.

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  • Post-treatment follow-up study of abdominal cystic echinococcosis in tibetan communities of northwest sichuan province, china Reviewed

    Li T., Ito A., Pengcuo R., Sako Y., Chen X., Qiu D., Xiao N., Craig PS

    PLoS Negl Trop Dis   5   e1364   2011.10

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  • Development and application of a loop-mediated isothermal amplification method for a rapid identification and differentiation of Taenia species from humans Reviewed

    A. Nkouawa, Y. Sako, T. Li, X. Chen, T. Wandra, I. Kadek Swastika, M. Nakao, T. Yanagida, M. Okamoto, P. Giraudoux

    TROPICAL MEDICINE & INTERNATIONAL HEALTH   16   170 - 170   2011.10

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  • A case of pulmonary and hepatic cystic Echinococcosis of CE1 stage in a healthy Japanese female that was suspected to have been acquired during her stay in the United Kingdom Reviewed

    Nakamura K., Ito A., Yara S., Haranaga S., Hibiya K., Hirayasu T., Sako Y., Fujita J.

    Am J Trop Med Hyg   85   456 - 459   2011.9

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  • Case Report: A Case of Pulmonary and Hepatic Cystic Echinococcosis of CE1 Stage in a Healthy Japanese Female that Was Suspected to Have Been Acquired During Her Stay in the United Kingdom Reviewed

    Kiwamu Nakamura, Akira Ito, Satomi Yara, Shusaku Haranaga, Kenji Hibiya, Tsuneo Hirayasu, Yasuhito Sako, Jiro Fujita

    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE   85 ( 3 )   456 - 459   2011.9

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  • Immunochromatographic test with recombinant Em18 antigen for the follow-up study of alveolar echinococcosis. Reviewed International journal

    Yasuhito Sako, Dennis Tappe, Kenta Fukuda, Yukuharu Kobayashi, Sonoyo Itoh, Matthias Frosch, Beate Grüner, Peter Kern, Akira Ito

    Clinical and vaccine immunology : CVI   18 ( 8 )   1302 - 5   2011.8

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    The performance of a rapid and simple immunochromatographic test (ICT) with recombinant Em18 (rEm18) antigen for serological follow-up of Echinococcus multilocularis infection was evaluated by comparison with that of an enzyme-linked immunosorbent assay (ELISA) with rEm18, using serum samples from patients who underwent surgery and/or received antiparasitic chemotherapy. The degree of Em18-band intensity on the ICT correlated highly with the absorbance value obtained by the ELISA. The kinetics of antibody levels obtained by the ICT paralleled those of the ELISA. These data suggest that the ICT has high potential as an easy-to-handle, fast, and reliable follow-up tool to monitor the status of alveolar echinococcosis in different stages.

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  • Should possible recurrence of disease contraindicate liver transplantation in patients with end-stage alveolar echinococcosis? A 20-year follow-up study Reviewed

    Bresson-Hadni S., Blagosklonov O., Knapp J., Grenouillet F., Sako Y., Delabrousse E., Brientini MP, Richou C., Minello A., Antonino AT, Gillet M., Ito A., Mantion GA, Vuitton DA

    Liver Transpl   17   855 - 865   2011.7

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  • Neurocysticercosis case with tuberculoma-like epithelial granuloma strongly suspected by serology and confirmed by mitochondrial DNA. Reviewed

    Maeda T., Ito A., Sako Y., Yamasaki H., Oyaizu N., OdawaraT., Iwamoto A., Fujii T.

    BMJ Case Reports   2011.7

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  • The first workshop towards the control of cestode zoonoses in Asia and Africa. International journal

    Akira Ito, Munehiro Okamoto, Tiaoying Li, Toni Wandra, Nyoman S Dharmawan, Kadek I Swastika, Paron Dekumyoy, Teera Kusolsuk, Abmed Davvajav, Anu Davaasuren, Temuulen Dorjsuren, Sissay M Mekonnen, Zerihun H Negasi, Tetsuya Yanagida, Yasuhito Sako, Minoru Nakao, Kazuhiro Nakaya, Antti J Lavikainen, Agathe Nkouawa, Tahereh Mohammadzadeh

    Parasites & vectors   4   114 - 114   2011.6

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    The first workshop towards the control of cestode zoonoses in Asia and Africa was held in Asahikawa Medical University, Japan on 15 and 16 Feb 2011. This meeting was fully supported by the Asian Science and Technology Strategic Cooperation Promotion Programs sponsored by the Special Coordination Funds for Promoting Science and Technology, the Ministry of Education Japan (MEXT) for 3 years from 2010 to Akira Ito. A total of 24 researchers from 9 countries joined together and discussed the present situation and problems towards the control of cestode zoonoses. As the meeting was simultaneously for the establishment of joint international, either bilateral or multilateral collaboration projects, the main purposes were directed to 1) how to detect taeniasis/cysticercosis infected patients, 2) how to differentiate Taenia solium from two other human Taenia species, T. saginata and T. asiatica, 3) how to evaluate T. asiatica based on the evidence of hybrid and hybrid-derived adult tapeworms from Thailand and China, 4) how to evaluate T. solium and T. hyaenae and other Taenia species from the wild animals in Ethiopia, and 5) how to detect echinococcosis patients and 6) how to differentiate Echinococcus species worldwide. Such important topics are summarized in this meeting report.

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  • Echinococcus multilocularis: Identification and functional characterization of cathepsin B-like peptidases from metacestode Reviewed

    Yasuhito Sako, Kazuhiro Nakaya, Akira Ito

    EXPERIMENTAL PARASITOLOGY   127 ( 3 )   693 - 701   2011.3

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  • A possible nuclear DNA marker to differentiate the two geographic genotypes of Taenia solium tapeworms Reviewed

    Marcello Otake Sato, Yasuhito Sako, Minoru Nakao, Toni Wandra, Kazuhiro Nakaya, Tetsuya Yanagida, Akira Ito

    PARASITOLOGY INTERNATIONAL   60 ( 1 )   108 - 110   2011.1

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    DOI： 10.1016/j.parint.2010.11.004

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  • Usefulness of serological and molecular tools for detection of neurologic parasitic zoonoses in rural areas of southwest Cameroon: toxocariasis, cysticercosis and paragonimiasis. Reviewed

    Nkouawa A., Sako Y., Moyou-Somo R., Ito A.

    Southeast Asian J Trop Med Public Health   42   1365 - 1374   2011

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  • Taeniasis/cysticercosis in Bali, Indonesia Reviewed

    Wandra T., Sudewi RAA, Swastika IK, Sutisna P., Dharmawan NS, Yulfi H., Darlan DM, Kapti IN, Samaan G., Sato MO, Okamoto M., Sako Y., Ito A.

    Southeast Asian J Trop Med Public Health   42   793 - 802   2011

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  • Neurocysticercosis case with tuberculoma-like epithelioid granuloma strongly suspected by serology and confirmed by mitochondrial DNA Reviewed

    Takuya Maeda, Akira Ito, Yasuhito Sako, Hiroshi Yamasaki, Naoki Oyaizu, Takashi Odawara, Aikichi Iwamoto, Takeshi Fujii

    BMJ Case Reports   15   bcr0420114125   2011

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    DOI： 10.1136/bcr.04.2011.4125

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  • Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand Reviewed

    Malinee T. Anantaphruti, Munehiro Okamoto, Tippayarat Yoonuan, Surapol Saguankiat, Teera Kusolsuk, Megumi Sato, Marcello O. Sato, Yasuhito Sako, Jitra Waikagul, Akira Ito

    PARASITOLOGY INTERNATIONAL   59 ( 3 )   326 - 330   2010.9

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    DOI： 10.1016/j.parint.2010.03.007

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  • Evaluation of a Loop-Mediated Isothermal Amplification Method Using Fecal Specimens for Differential Detection of Taenia Species from Humans Reviewed

    Agathe Nkouawa, Yasuhito Sako, Tiaoying Li, Xingwang Chen, Toni Wandra, I. Kadek Swastika, Minoru Nakao, Tetsuya Yanagida, Kazuhiro Nakaya, Dongchuan Qiu, Akira Ito

    JOURNAL OF CLINICAL MICROBIOLOGY   48 ( 9 )   3350 - 3352   2010.9

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    DOI： 10.1128/JCM.00697-10

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  • Serological studies of neurologic helminthic infections in rural areas of southwest cameroon: toxocariasis, cysticercosis and paragonimiasis. Reviewed International journal

    Agathe Nkouawa, Yasuhito Sako, Sonoyo Itoh, Alida Kouojip-Mabou, Christ Nadège Nganou, Yasuaki Saijo, Jenny Knapp, Hiroshi Yamasaki, Minoru Nakao, Kazuhiro Nakaya, Roger Moyou-Somo, Akira Ito

    PLoS neglected tropical diseases   4 ( 7 )   e732   2010.7

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    BACKGROUND: Both epilepsy and paragonimiasis had been known to be endemic in Southwest Cameroon. A total of 188 people (168 and 20 with and without symptoms confirmed by clinicians, respectively, 84.6% under 20 years old) were selected on a voluntary basis. Among 14 people (8.3%) with history of epilepsy, only one suffered from paragonimiasis. Therefore, we challenged to check antibody responses to highly specific diagnostic recombinant antigens for two other helminthic diseases, cysticercosis and toxocariasis, expected to be involved in neurological diseases. Soil-transmitted helminthic infections were also examined. METHODOLOGY/PRINCIPAL FINDINGS: Fecal samples were collected exclusively from the 168 people. Eggs of Ascaris lumbricoides, Trichuris trichiura and hookworms were found from 56 (33.3%), 72 (42.8%), and 19 (11.3%) persons, respectively. Serology revealed that 61 (36.3%), 25 (14.9%) and 2 (1.2%) of 168 persons showed specific antibody responses to toxocariasis, paragonimiasis and cysticercosis, respectively. By contrast, 20 people without any symptoms as well as additional 20 people from Japan showed no antibody responses. Among the 14 persons with epilepsy, 5 persons were seropositive to the antigen specific to Toxocara, and one of them was simultaneously positive to the antigens of Paragonimus. The fact that 2 children with no history of epilepsy were serologically confirmed to have cysticercosis strongly suggests that serological survey for cysticercosis in children is expected to be useful for early detection of asymptomatic cysticercosis in endemic areas. CONCLUSIONS/SIGNIFICANCE: Among persons surveyed, toxocariasis was more common than paragonimiasis, but cysticercosis was very rare. However, the fact that 2 children were serologically confirmed to have cysticercosis was very important, since it strongly suggests that serology for cysticercosis is useful and feasible for detection of asymptomatic cysticercotic children in endemic areas for the early treatment.

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  • Immunoglobulin G subclass responses to recombinant Em18 in the follow-up of patients with alveolar echinococcosis in different clinical stages Reviewed

    Tappe D., Sako Y., Itoh S., Frosch M., Gruner B., Kern P., Ito A.

    Clin Vaccine Immunol   17   944 - 948   2010.6

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    DOI： 10.1128/CVI.00026-10

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  • Neurocysticercosis: assessing where the infection was acquired from Reviewed

    Yanagida T., Yuzawa I., Joshi DD, Sako Y., Nakao M., Nakaya K., Kawano N., Oka H., Fujii K., Ito A.

    J Travel Med   17   206 - 208   2010.5

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  • State-of-the-art Echinococcus and Taenia: Phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis Reviewed

    M. Nakao, T. Yanagida, M. Okamoto, J. Knapp, A. Nkouawa, Y. Sako, A. Ito

    Infection, Genetics and Evolution   10 ( 4 )   444 - 452   2010.5

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  • Specific IgG Responses to Recombinant Antigen B and Em18 in Cystic and Alveolar Echinococcosis in China Reviewed

    Tiaoying Li, Akira Ito, Xingwang Chen, Yasuhito Sako, Jiamin Qiu, Ning Xiao, Dongchuan Qiu, Minoru Nakao, Tetsuya Yanagida, Philip S. Craig

    CLINICAL AND VACCINE IMMUNOLOGY   17 ( 3 )   470 - 475   2010.3

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    DOI： 10.1128/CVI.00466-09

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  • Short Report: Histopathological, Serological, and Molecular Confirmation of Indigenous Alveolar Echinococcosis Cases in Mongolia Reviewed

    Akira Ito, Gurbadam Agvaandaram, Oyun-Erdene Bat-Ochir, Batsaikhan Chuluunbaatar, Nyamkhuu Gonchigsenghe, Tetsuya Yanagida, Yasuhito Sako, Narankhajid Myadagsuren, Temuulen Dorjsuren, Kazuhiro Nakaya, Minoru Nakao, Yuji Ishikawa, Abmed Davaajav, Nyamkhuu Dulmaa

    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE   82 ( 2 )   266 - 269   2010.2

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    DOI： 10.4269/ajtmh.2010.09-0520

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  • Echinococcus vogeli infection in a hunter, French Guiana. Reviewed

    Knapp J., Chirica M., Simonnet C., Grenouillet F., Bart JM, Sako Y., Itoh S., Nakao M., Ito A., Millon L.

    Emerg Infect Dis   15   2029 - 2031   2009.12

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  • Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis Reviewed

    M. Nakao, N. Xiao, M. Okamoto, T. Yanagida, Y. Sako, A. Ito

    Parasitology International   58 ( 4 )   384 - 389   2009.12

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  • Serological monitoring of progression of alveolar echinococcosis with multiorgan involvement by use of recombinant Em18. Reviewed

    Ishikawa Y., Sako Y., Itoh S., Ohtake T., Kohgo Y., Matsuno T., Ohsaki Y., Miyokawa N., Nakao M., Nakaya K., Ito A.

    J Clin Microbiol   47   3191 - 3196   2009.10

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  • Close relationship between clinical regression and specific serology in the follow-up of patients with alveolar echinococcosis in different clinical stages. Reviewed

    Tappe D., Frosch M., Sako Y., Itoh S., Gruner B., Reuter S., Nakao M., Ito A., Kern P.

    Am J Trop Med Hyg   80   792 - 797   2009.5

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  • Widespread co-endemicity of human cystic and alveolar echinococcosis on the eastern Tibetan Plateau, northwest Sichuan/southeast Qinghai, China. Reviewed

    Li T., Chen X., Zhen R., Qiu J., Qiu D., Xiao N., Ito A., Wang H., Giraudoux P., Sako Y., Nakao M., Craig PS

    Acta Trop   113 ( (3) )   248 - 256   2009.3

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  • Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations Reviewed

    A. Nkouawa, M. Okamoto, A.K. Mabou, E. Edinga, H. Yamasaki, Y. Sako, M. Nakao, K. Nakaya, D. Blair, T. Agatsuma, P. Enyong, T. Shibahara, R. Moyou-Somo, A. Ito

    Transactions of the Royal Society of Tropical Medicine and Hygiene   103 ( 3 )   255 - 261   2009.3

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  • Development of an Immunochromatographic Test To Detect Antibodies against Recombinant Em18 for Diagnosis of Alveolar Echinococcosis Reviewed

    Yasuhito Sako, Kenta Fukuda, Yukuharu Kobayashi, Akira Ito

    JOURNAL OF CLINICAL MICROBIOLOGY   47 ( 1 )   252 - 254   2009.1

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    DOI： 10.1128/JCM.01476-08

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  • Loop-Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species Reviewed

    Agathe Nkouawa, Yasuhito Sako, Minoru Nakao, Kazuhiro Nakaya, Akira Ito

    JOURNAL OF CLINICAL MICROBIOLOGY   47 ( 1 )   168 - 174   2009.1

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    DOI： 10.1128/JCM.01573-08

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  • Genetic characterization and phylogenetic position of Echinococcus felidis Ortlepp, 1937 (Cestoda : Taeniidae) from the African lion Reviewed

    Marion Huettner, Minoru Nakao, Torsten Wassermann, Ludwig Siefert, Joop D. F. Boomker, Anke Dinkel, Yasuhito Sako, Ute Mackenstedt, Thomas Romig, Akira Ito

    INTERNATIONAL JOURNAL FOR PARASITOLOGY   38 ( 7 )   861 - 868   2008.6

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    DOI： 10.1016/j.ijpara.2007.10.013

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  • Molecular characterization of a novel gene encoding an 8-kDa-subunit of antigen B from Echinococcus granulosus genotypes 1 and 6 Reviewed

    Mamuti Wulamu, Yasuhito Sako, Jean-Mathieu Bart, Minoru Nakao, Xiurnin Ma, Hao Wen, Akira Ito

    PARASITOLOGY INTERNATIONAL   56 ( 4 )   313 - 316   2007.12

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    DOI： 10.1016/j.parint.2007.06.003

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  • Sympatric occurrence of Taenia solium, T-saginata, and T-asiatica, Thailand Reviewed

    Malinee T. Anantaphruti, Hiroshi Yamasaki, Minoru Nakao, Jitra Waikagul, Dorn Watthanakulpanich, Supaporn Nuamtanong, Wanna Maipanich, Somchit Pubampen, Surapol Sanguankiat, Chatree Muennoo, Kazuhiro Nakaya, Marcello O. Sato, Yasuhito Sako, Munehiro Okamoto, Akira Ito

    EMERGING INFECTIOUS DISEASES   13 ( 9 )   1413 - 1416   2007.9

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    DOI： 10.3201/eid1309.061148

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  • Comparison of several commercial serologic kits and Em18 serology for detection of human alveolar echinococcosis Reviewed

    Jean-Mathieu Bart, Martine Piarroux, Yasuhito Sako, Frederic Grenouillet, Solange Bresson-Hadni, Renaud Piarroux, Akira Ito

    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE   59 ( 1 )   93 - 95   2007.9

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    DOI： 10.1016/j.diagmicrobio.2007.03.018

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  • Taeniasis in Mongolia, 2002-2006. Reviewed

    Myadagsuren N., Davaajav A., Wandra T., Sandar T., Ichinkhorloo P., Yamasaki H., Sako Y., Nakao M., Sato MO, Nakaya K., Ito A.

    Am J Trop Med Hyg   77   342 - 346   2007.8

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  • Cloning and characterization of cathepsin L-like peptidases of Echinococcus multilocularis metacestodes Reviewed

    Yasuhito Sako, Hiroshi Yamasaki, Kazuhiro Nakaya, Minoru Nakao, Akira Ito

    MOLECULAR AND BIOCHEMICAL PARASITOLOGY   154 ( 2 )   181 - 189   2007.8

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    DOI： 10.1016/j.molbiopara.2007.04.016

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  • Echinococcosis: serological detection of patients and molecular identification of parasites

    Akira Ito, Minoru Nakao, Yasuhito Sako

    FUTURE MICROBIOLOGY   2 ( 4 )   439 - 449   2007.8

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  • Current Situation of Taeniasis and Cysticercosis in Indonesia Reviewed

    Toni Wandra, Sri S. Margono, Made S. Gafar, John M Saragih, Putu Sutisna, A.A. Raka Sudewi, A.A. Depary, Hemma Yulfi, Dewi Masyithah Darla, Munehiro Okamoto, Marcello Otake Sato, Yasuhito Sako, Minoru Nakao, Kazuhiro Nakaya, Philip S. Craig, Akira Ito

    Trop.Med.Health   35 ( 4 )   323 - 328   2007

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    DOI： 10.2149/tmh.35.323

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  • Significance of Molecular Diagnosis using Histopathological Specimens in Cestode Zoonoses

    Hiroshi Yamasaki, Kazuhiro Nakaya, Minoru Nakao, Yasuhito Sako, Akira Ito

    Tropical Medicine and Health   35 ( 4 )   307 - 321   2007

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    DOI： 10.2149/tmh.35.307

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  • Evaluation of purified Taenia solium glycoproteins and recombinant antigens in the serologic detection of human and swine cysticercosis Reviewed

    Marcello Otake Sato, Yasuhito Sako, Minoru Nakao, Hiroshi Yamasaki, Kazuhiro Nakaya, Akira Ito

    JOURNAL OF INFECTIOUS DISEASES   194 ( 12 )   1783 - 1790   2006.12

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    DOI： 10.1086/509262

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    Other Link： http://orcid.org/0000-0002-9204-0602

  • Short report: Evidence and potential for transmission of human and swine Taenia solium cysticercosis in the Piracuruca region, Piaui, Brazil. Reviewed

    Sato MO, Cavalcante TV, Sako Y., Nakao M., Yamasaki H., Yatsuda AP, Nakaya K., Ito A.

    Am J Trop Med Hyg   75   933 - 935   2006.11

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  • Echinococcus multilocularis: developmental stage-specific expression of Antigen B 8-kDa-subunits. Reviewed

    Mamuti W., Sako Y., Xiao N., Nakaya K., Nakao M., Yamasaki H., Lightowlers MW, Craig PS, Ito A.

    Exp Parasitol   113   75 - 82   2006.6

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  • A case of intramuscular cysticercosis diagnosed definitively by mitochondrial DNA analysis of extremely calcified cysts Reviewed

    H Yamasaki, T Nagase, Y Kiyoshige, M Suzuki, K Nakaya, Y Itoh, Y Sako, M Nakao, A Ito

    PARASITOLOGY INTERNATIONAL   55 ( 2 )   127 - 130   2006.6

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  • Towards the international collaboration for detection, surveillance and control of taeniasis/ cysticercosis and echinococcosis in Asia and the Pacific. Reviewed

    Ito A., Wandra T., Sato MO, Mamuti W., Xiao N., Sako Y., Nakao M., Yamasaki H., Nakaya K., Okamoto M., Craig PS

    Southeast Asian J Trop Med Public Health   37 ( Suppl 3 )   82 - 90   2006.4

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  • Mitochondrial DNA diagnosis for taeniasis and cysticercosis. Reviewed

    Yamasaki H., Nakao M., Sako Y., Nakaya K., Sato MO, Ito A.

    Parasitol Int   55 ( Suppl )   S81 - 85   2006.4

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  • Recent advances in characterization of Echinococcus antigen B. Reviewed

    Mamuti W., Sako Y., Nakao M., Xiao N., Nakaya K., Ishikawa Y., Yamasaki H., Lightowlers MW, Ito A.

    Parasitol Int   55   S57 - 62   2006.4

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  • Usefulness of severe combined immunodeficiency (scid) and inbred mice for studies of cysticercosis and echinococcosis. Reviewed

    Nakaya K., Mamuti W., Xiao N., Sato MO, Wandra T., Nakao M., Sako Y., Yamasaki H., Ishikawa Y., Craig PS, Schantz PM, Ito A.

    Parasitol Int   55 ( Suppl )   S91 - 97   2006.4

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  • Neurocysticercosis: clinical manifestation, neuroimaging, serology and molecular confirmation of histopathologic specimens. Reviewed

    Ito A., Takayanagui OM, Sako Y., Sato MO, Odashima NS, Yamasaki H., Nakaya K., Nakao M.

    Southeast Asian J Trop Med Public Health   37 ( Suppl 3 )   74 - 81   2006.4

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  • Recombinant antigens for serodiagnosis of cysticercosis and echinococcosis Reviewed

    Y Sako, M Nakao, K Nakaya, H Yamasaki, A Ito

    PARASITOLOGY INTERNATIONAL   55   S69 - S73   2006

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  • Evaluation of clinical and serological data from Taenia solium cysticercosis patients in eastern Inner Mongolia Autonomous Region, China Reviewed

    T Ikejima, ZX Piao, Y Sako, MO Sato, S Bao, R Si, F Yu, CL Zhang, M Nakao, H Yamasaki, K Nakaya, T Kanazawa, A Ito

    TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE   99 ( 8 )   625 - 630   2005.8

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  • Taeniasis and cysticercosis in Asia and the Pacific: present state of knowledge and perspectives. Reviewed

    Ito A., Nakao M., Wandra T., Suroso T., Okamoto M., Yamasaki H., Sako Y., Nakaya K.

    Southeast Asian J Trop Med Public Health   36 ( Suppl 4 )   123 - 30   2005.4

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  • Molecular identification of Taenia solium cysticercus genotype in the histopathological specimens. Reviewed

    Yamasaki H., Nakao M., Sako Y., Nakaya K., Ito A.

    Southeast Asian J Trop Med Public Health   36 ( Suppl 4 )   131 - 134   2005.4

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  • Short report: Echinococcus multilocularis confirmed on Kunashiri Island, 15 kilometers from the eastern part of Hokkaido, Japan. Reviewed

    Satoh M., Nakaya K., Nakao M., Xiao N., Yamasaki H., Sako Y., Naitoh Y., Kondo S., Kobayashi M., Ohtaishi N., Ito A.

    Am J Trop Med Hyg   72   284 - 288   2005.3

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  • Solitary neurocysticercosis case caused by Asian genotype of Taenia solium confirmed by mitochondrial DNA analysis. Reviewed

    Yamasaki H., Matsunaga S., Yamamura K., Chang CC, Kawamura S., Sako Y., Nakao M., Nakaya K., Ito A.

    J Clin Microbiol   42   3891 - 3893   2004.8

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    DOI： 10.1128/JCM.42.8.3891-3893.2004

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  • Cysticercosis/taeniasis in Asia and the Pacific. Reviewed

    Ito A., Wandra T., Yamasaki H., Nakao M., Sako Y., Nakaya K., Margono SS, Suroso T., Gauci C., Lightowlers MW

    Vector Borne Zoonotic Dis   4   95 - 107   2004.4

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  • Cysticercosis: IgG-ELISA evaluations of peak1 antigen and <30 kDa antigen of delipidized extract of Taenia solium metacestodes. Reviewed

    Dekumyoy P., Waikagul J., Vanijanonta S., Thairungroj M., Nakao M., Sako Y., Watanabe S., Ito A.

    Southeast Asian J Trop Med Public Health   35   1 - 9   2004.4

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  • Molecular cloning, expression, and serological evaluation of an, 8-kilodalton subunit of antigen B from Echinococcus multilocularis Reviewed

    W Mamuti, H Yamasaki, Y Sako, M Nakao, N Xiao, K Nakaya, N Sato, DA Vuitton, R Piarroux, MW Lightowlers, PS Craig, A Ito

    JOURNAL OF CLINICAL MICROBIOLOGY   42 ( 3 )   1082 - 1088   2004.3

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    DOI： 10.1128/JCM.42.3.1082-1088.2004

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  • Cysticercosis: IgG-ELISA evaluations of peak1 antigen and &lt;30 kDa antigen of delipidized extract of Taenia solium metacestodes

    Paron Dekumyoy, Jitra Waikagul, Sirivan Vanijanonta, Malinee Thairungroj, Minoru Nakao, Yasuhito Sako, Sonoyo Watanabe, Akira Ito

    Southeast Asian Journal of Tropical Medicine and Public Health   35 ( 1 )   1 - 9   2004.3

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  • DNA differential diagnosis of taeniasis and cysticercosis by multiplex PCR Reviewed

    H Yamasaki, JC Allan, MO Sato, M Nakao, Y Sako, K Nakaya, DC Qiu, W Mamuti, PS Craig, A Ito

    JOURNAL OF CLINICAL MICROBIOLOGY   42 ( 2 )   548 - 553   2004.2

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    DOI： 10.1128/JCM.42.2.548-553.2004

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  • Short report: Identification of Echinococcus species from a Yak in the Qinghai-Tibet Plateau Region of China Reviewed

    N Xiao, RM Qiu, M Nakao, K Nakaya, H Yamasaki, Y Sako, W Mamuti, PM Schantz, PS Craig, A Ito

    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE   69 ( 4 )   445 - 446   2003.10

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  • Isolation of polymorphic microsatellite loci from the tapeworm Echinococcus multilocularis Reviewed

    Minoru Nakao, Yasuhito Sako, Akira Ito

    INFECTION GENETICS AND EVOLUTION   3 ( 3 )   159 - 163   2003.9

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    DOI： 10.1016/S1567-1348(03)00070-4

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  • Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections Reviewed

    N Xiao, W Mamuti, H Yamasaki, Y Sako, M Nakao, K Nakaya, B Gottstein, PM Schantz, MW Lightowlers, PS Craig, A Ito

    JOURNAL OF CLINICAL MICROBIOLOGY   41 ( 7 )   3351 - 3353   2003.7

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    DOI： 10.1128/JCM.41.7.3351-3353.2003

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  • Multiple genotypes of Taenia solium - ramifications for diagnosis, treatment and control Reviewed

    A Ito, H Yamasaki, M Nakao, Y Sako, M Okamoto, MO Sato, K Nakaya, SS Margono, T Ikejima, AA Kassuku, SMS Afonso, WB Ortiz, A Plancarte, A Zoli, S Geerts, PS Craig

    ACTA TROPICA   87 ( 1 )   95 - 101   2003.6

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    DOI： 10.1016/S0001-706X(03)00024-X

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  • The mitochondrial genome of the tapeworm Taenia solium: A finding of the abbreviated stop codon U Reviewed

    M Nakao, Y Sako, A Ito

    JOURNAL OF PARASITOLOGY   89 ( 3 )   633 - 635   2003.6

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  • Evaluation of tongue inspection and serology for diagnosis of Taenia solium cysticercosis in swine: Usefulness of ELISA using purified glycoproteins and recombinant antigen Reviewed

    M. O. Sato, H. Yamasaki, Y. Sako, M. Nakao, K. Nakaya, A. Plancarte, A. A. Kassuku, P. Dorny, S. Geerts, W. Benitez-Ortiz, Y. Hashiguchi, A. Ito

    Veterinary Parasitology   111 ( 4 )   309 - 322   2003.2

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    DOI： 10.1016/S0304-4017(02)00383-7

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  • Development of Em18-immunoblot and Em18-ELISA for specific diagnosis of alveolar echinococcosis Reviewed

    A Ito, Y Sako, H Yamasaki, W Mamuti, K Nakaya, M Nakao, Y Ishikawa

    ACTA TROPICA   85 ( 2 )   173 - 182   2003.2

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  • Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: Results of a blind test Reviewed

    A Ito, N Xiao, M Liance, MO Sato, Y Sako, W Mamuti, Y Ishikawa, M Nakao, H Yamasaki, K Nakaya, K Bardonnet, S Bresson-Hadni, DA Vuitton

    JOURNAL OF CLINICAL MICROBIOLOGY   40 ( 11 )   4161 - 4165   2002.11

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    DOI： 10.1128/JCM.40.11.4161-4165.2002

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    Other Link： http://orcid.org/0000-0002-9204-0602

  • DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes Reviewed

    H. Yamasaki, M. Nakao, Y. Sako, K. Nakaya, M.O. Sato, W. Mamuti, M. Okamoto, A. Ito

    Journal of Clinical Microbiology   40 ( 10 )   3818 - 3821   2002.10

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  • The complete mitochondrial DNA sequence of the cestode Echinococcus multilocularis (Cyclophyllidea : Taeniidae) Reviewed

    M Nakao, N Yokoyama, Y Sako, M Fukunaga, A Ito

    MITOCHONDRION   1 ( 6 )   497 - 509   2002.10

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  • Alveolar echinococcosis: Characterization of diagnostic antigen Em18 and serological evaluation of recombinant Em18 Reviewed

    Y Sako, M Nakao, K Nakaya, H Yamasaki, B Gottstein, MW Lightowers, PM Schantz, A Ito

    JOURNAL OF CLINICAL MICROBIOLOGY   40 ( 8 )   2760 - 2765   2002.8

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    DOI： 10.1128/JCM.40.8.2760-2765.2002

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  • Usefulness of hydatid cyst fluid of Echinococcus granulosus developed in mice with secondary infection for serodiagnosis of cystic echinococcosis in humans Reviewed

    W Mamuti, H Yamasaki, Y Sako, K Nakaya, M Nakao, MW Lightowlers, A Ito

    CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY   9 ( 3 )   573 - 576   2002.5

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    DOI： 10.1128/CDLI.9.3.573-576.2002

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  • Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis Reviewed

    A. Ito, M.I. Putra, R. Subahar, M.O. Sato, M. Okamoto, Y. Sako, M. Nakao, H. Yamasaki, K. Nakaya, P.S. Craig, S.S. Margono

    Journal of Helminthology   76 ( 4 )   311 - 314   2002

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    DOI： 10.1079/JOH2002128

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  • Differential serodiagnosis for alveolar echinococcosis by Em18-immunoblot and Em18-ELISA in Japan and China Reviewed

    A Ito, Y Sako, Y Ishikawa, M Nakao, K Nakaya, H Yamasaki

    CESTODE ZOONOSES: ECHINOCOCCOSIS AND CYSTICERCOSIS   341   147 - 155   2002

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  • Neurocysticercosis in Asia: Serology/seroepidemiology in humans and pigs Reviewed

    A Ito, Y Sako, M Nakao, K Nakaya

    CESTODE ZOONOSES: ECHINOCOCCOSIS AND CYSTICERCOSIS   341   25 - 31   2002

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  • A phylogenetic hypothesis for the distribution of two genotypes of the pig tapeworm Taenia solium worldwide Reviewed

    M. Nakao, M. Okamoto, Y. Sako, H. Yamasaki, K. Nakaya, A. Ito

    Parasitology   124 ( 6 )   657 - 662   2002

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    DOI： 10.1017/S0031182002001725

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  • Taeniasis/cysticercosis in Papua (Irian Jaya), Indonesia Reviewed

    A Ito, SS Margono, R Subahar, A Hamid, T Wandra, T Suroso, MO Sato, H Yamasaki, M Nakao, Y Sako, K Nakaya, M Okamoto, PS Craig

    PROCEEDINGS OF THE 10TH INTERNATIONAL CONGRESS OF PARASITOLOGY-ICOPA X: SYMPOSIA, WORKSHOPS AND CONTRIBUTED PAPERS   207 - 211   2002

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  • DNA differential diagnosis of human taeniid cestodes using restriction fragment length polymorphism and base excision sequence scanning T-base system Reviewed

    H Yamasaki, M Nakao, Y Sako, K Nakaya, MO Sato, W Mamuti, N Xiao, M Okamoto, A Ito

    PROCEEDINGS OF THE 10TH INTERNATIONAL CONGRESS OF PARASITOLOGY-ICOPA X: SYMPOSIA, WORKSHOPS AND CONTRIBUTED PAPERS   189 - 193   2002

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  • Comparison of the antigenicity of protoscoleces and microvesicles of Echinococcus multilocularis prepared from rats Reviewed

    A Ito, T Kanazawa, M Nakao, Y Sako, Y Ishikawa, K Nakaya

    JOURNAL OF HELMINTHOLOGY   75 ( 4 )   355 - 358   2001.12

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  • Taenia solium infection in Irian Jaya (West Papua), Indonesia: a pilot serological survey of human and porcine cysticercosis in Jayawijaya District Reviewed

    R Subahar, A Hamid, W Purba, T Wandra, C Karma, Y Sako, SS Margono, PS Craig, A Ito

    TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE   95 ( 4 )   388 - 390   2001.7

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  • Molecular variation of Taenia solium in the world Reviewed

    M. Okamoto, M. Nakao, Y. Sako, A. Ito

    Southeast Asian Journal of Tropical Medicine and Public Health   32 ( SUPPL. 2 )   90 - 93   2001

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  • NOD-scid mouse as an experimental animal model for cysticercosis Reviewed

    Akira Ito, Kazuhiro Nakaya, Yasuhito Sako, Minoru Nakao, Mamoru Ito

    Southeast Asian Journal of Tropical Medicine and Public Health   32 ( 2 )   85 - 89   2001

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  • Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes Reviewed

    Y Sako, M Nako, T Ikejima, XZ Piao, K Nakaya, A Ito

    JOURNAL OF CLINICAL MICROBIOLOGY   38 ( 12 )   4439 - 4444   2000.12

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  • Mitochondrial genetic code in cestodes Reviewed

    M Nakao, Y Sako, N Yokoyama, M Fukunaga, A Ito

    MOLECULAR AND BIOCHEMICAL PARASITOLOGY   111 ( 2 )   415 - 424   2000.12

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    DOI： 10.1016/S0166-6851(00)00334-0

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  • Resurgence of cases of epileptic seizures and burns associated with cysticercosis in Assologaima, Jayawijaya, Irian Jaya, Indonesia, 1991-95 Reviewed

    T Wandra, R Subahar, GM Simanjuntak, SS Margono, T Suroso, M Okamoto, M Nakano, Y Sako, K Nakaya, PM Schantz, A Ito

    TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE   94 ( 1 )   46 - 50   2000.1

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    DOI： 10.1016/S0035-9203(00)90433-4

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  • Neurocysticercosis and echinococcosis in Asia: Recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration Reviewed

    Akira Ito, M. Nakao, Y. Sako, K. Nakaya

    Southeast Asian Journal of Tropical Medicine and Public Health   31 ( 1 )   16 - 20   2000

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  • Molecular cloning and characterisation of 23-kDa piroplasm surface proteins of Theileria sergenti and Theileria buffeli Reviewed

    Y Sako, M Asada, S Kubota, C Sugimoto, M Onuma

    INTERNATIONAL JOURNAL FOR PARASITOLOGY   29 ( 4 )   593 - 599   1999.4

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    DOI： 10.1016/S0020-7519(99)00004-1

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  • Expression of a major piroplasm surface protein of Theileria sergenti in sporozoite stage Reviewed

    Y Sako, C Sugimoto, M Onuma

    JOURNAL OF VETERINARY MEDICAL SCIENCE   61 ( 3 )   275 - 277   1999.3

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    DOI： 10.1292/jvms.61.275

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  • Cloning of a cysteine proteinase gene of Theileria sergenti Reviewed

    Y Sako, C Sugimoto, M Onuma

    JOURNAL OF VETERINARY MEDICAL SCIENCE   61 ( 3 )   271 - 273   1999.3

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    DOI： 10.1292/jvms.61.271

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Books

  • 近年話題になっている新興・再興感染症・One Healthとしての感染症：エキノコックス症

    迫 康仁（ Role： Sole author）

    北海道医師会  2025.11 

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  • コンパクト微生物学（寄生虫）

    迫 康仁（ Role： Sole author）

    南江堂  2021.3 

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  • シンプル微生物学（原虫学・蠕虫学）

    迫 康仁（ Role： Sole author）

    南江堂  2018.3 

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  • エキノコックス症（単包条虫症、多包条虫症）

    迫 康仁（ Role： Sole author）

    医薬ジャーナル社  2016.2 

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  • 有鉤嚢虫症の免疫検査抗原の簡便な精製法

    迫 康仁, 岡本宗裕, 伊藤 亮（ Role： Joint author）

    三恵社  2014 

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  • 臨床検査ガイド：抗エキノコックス抗体

    伊藤 亮, 迫 康仁（ Role： Joint author）

    文光堂  2013.3 

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  • Loop-mediated isothermal amplification method for a differential identification of human taenia tapeworms.

    Sako Y., Nkouawa A., Yanagida T., Ito A.（ Role： Joint author）

    Humana Press  2013 

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  • 教科書には載っていない 臨床検査Q＆A:：糞便中の寄生虫の核酸検査法について教えてください

    迫 康仁, 伊藤 亮（ Role： Joint author）

    医学書院  2012.10 

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  • Chapter Echinococcus and Echinococcosis. In: Molecular Detection of Human Parasitic Pathogens (ed. by Liu D)

    Ito A., Nakao M., Sako Y., Yanagida T., Nakaya K., Knapp J., Ishikawa Y.（ Role： Joint author）

    CRC Press  2012 

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  • Chapter 62. Taeniasis. In: Molecular Detection of Foodbone Pathogens (ed, Liu D)

    Ito A., Nakao M., Sako Y., Nakaya K., Okamoto M., Yanagida T.（ Role： Joint author）

    CRC press  2010.9 

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Presentations

  • 多包虫症の新規検査法の標的としての宿主血中循環セルフリーDNAの評価

    入江 隆夫, 伊東 拓也, 後藤 明子, 中尾 亮, 迫 康仁, 水村 匡伸, 長野 礼太郎, 吉田 彩子, 山野 公明

    第94回寄生虫学会大会  2025.3 

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    Presentation type：Oral presentation (general)  

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  • Soil eDNA opening new insights in O.h.quadrasi distribution and S. japonicum environmental presence in a community level

    JOSEPH EVANGELISTA VALENCIA, Marcello Otake Sato, Mark June, Revolteado, Phoyphaylinh Prasayasith, Eleonor A Cervantes, Ralph N Aniceto, Mario L Jiz, Yasuhito Sako, Lydia R Leonardo, Yuichi Chigusa, Megumi Sato

    2025.3 

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    Event date： 2025.3

    Presentation type：Oral presentation (general)  

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  • Community Level Application of eDNA Surveillance System:Assessing & Mapping the Temporality of Schistosomiasis in Ekiran, Leyte, Philippines

    Mark June Valiente, Revolteado, Marcello Otake Sato, Joseph Evangelista Valencia, Mario Jiz, Eleonor Cervantes, Ralph Aniceto, Yasuhito Sako, Megumi Sato

    2025.3 

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    Presentation type：Oral presentation (general)  

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  • Seasonality of schistosomiasis infection risk and the dynamics of environmental DNA detection

    Marcello Otake Sato, Megumi Sato, Joseph Valencia, Phoyphaylinh Prasayasith, Mark June Revolteado, Mario Jiz, Yasuhito Sako

    21st International Congress for Tropical Medicine and Malaria (ICTMM2024)  2024.9 

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    Event date： 2024.9

    Language：English   Presentation type：Symposium, workshop panel (public)  

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  • 環境DNA手法を用いたフィリピン、レイテ島、エキラン村における日本住血吸虫症の感染リスク評価

    ヴァレンシア ジョセフ, サトウ マルセロ オオタケ, パブロ アーチー, キムソン マリオ, セルバンテス エレノアー, ジズ マリオ, 迫 康仁, サトウ 恵

    第９３回日本寄生虫学会大会 

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    Event date： 2024.3

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 多包虫由来物質がヒトの肝細胞に及ぼす影響の解明

    伴戸 寛徳, 迫 康仁

    第９３回日本寄生虫学会大会 

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    Event date： 2024.3

    Language：Japanese   Presentation type：Poster presentation  

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  • 環境DNA手法を用いたフィリピン、レイテ島、エキラン村における日本住血吸虫中間宿主Oncomelania hupensis quadrasiのハザードマップ作成

    レヴォルテアド マーク, ジューン, サトウ マルセロ オオタケ, ジズ マリオ, セルバンテス エレノアー, パブロ アーチー, キムソン マリオ, 迫 康仁, サトウ 恵

    第９３回日本寄生虫学会大会 

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    Event date： 2024.3

    Language：English   Presentation type：Oral presentation (general)  

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  • Understanding mechanisms of Toxoplasma gondii stage conversion in human iPSC-derived neurons

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    Event date： 2023.3

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Next steps of eDNA towards the control of schistosomiasis International conference

    Maecello Otake Sato, サトウ 恵, 迫 康仁

    国際熱帯医学・マラリア学学会 

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    Event date： 2022.10

    Language：English   Presentation type：Oral presentation (general)  

    Venue：バンコク、タイ  

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  • Environmental DNA and risk mapping in schistosomiasis International conference

    Marcello Otake Sato, Megumi Sato, Yasuhito Sako

    International Congress of Parasitology 

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    Event date： 2022.8

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • エキノコックス症と条虫症

    迫康仁

    日本小児科学会北海道地方会第304回例会  日本小児科学会北海道地方会

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    Event date： 2019.2

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：旭川  

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  • 日本海裂頭条虫感染の1症例報告

    工藤裕基, 迫康仁

    第13回日本臨床検査学教育学術大会 

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    Event date： 2018.8

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • バリ島およびニューギニア島における有鉤条虫の遺伝的多様性とその起源

    柳田 哲矢, Swastika Kadek, Dharmawan Noman S., dra Toni, 佐藤 宏, 迫 康仁, 伊藤 亮, 岡本 宗裕

    第85回 日本寄生虫学会大会 

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    Event date： 2016.3

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 多包虫は宿主の補体活性を阻害する－多包虫セリンプロテアーゼインヒビターのはたらきー

    佐々木 瑞希, 迫 康仁

    第85回 日本寄生虫学会大会 

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    Event date： 2016.3

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Development of a simple identification of human taenia species by using multiplex LAMP and dot-ELISA

    Nkouawa Agathe, Sako Yaushito

    第84回 日本寄生虫学会大会 

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    Event date： 2015.3

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Diagnostics of Cysticercosis and Taeniasis International conference

    Yasuhito SAKO

    Taeniasis/Cysticercosis Symposium at Udayana University 

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    Event date： 2014.9

    Language：English   Presentation type：Oral presentation (invited, special)  

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  • イムノクロマトグラフィー法による有鉤嚢虫症の血清検査法の開発

    迫 康仁, 福田健太, 小林行治, 伊藤 亮

    第82回日本寄生虫学会大会 

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    Event date： 2013.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京  

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  • Advances in technology for EBM International conference

    Y. Sako, A. Ito

    Joint International Tropical Medicine Meeting 2012 

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    Event date： 2012.12

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

    Venue：Bangkok, Thailand  

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  • Recent advances in immunogiagnosis of cysticercosis International conference

    Y. Sako, A. Ito

    International Symposium on Cestode Zoonoses Controlo 

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    Event date： 2012.10

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Shanghai, CHina  

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  • イムノクロマトグラフィー法による多包虫の血清検査および経過観察検査について

    迫 康仁, Tappe Dennis, 福田健太, 小林行治, 伊藤園予, Frosch Matthias, Gruner Beate, Kern Peter, 伊藤 亮

    日本寄生虫学会総会 

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    Event date： 2012.3

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • An immunochromatographic test for the diagnosis and the follow-up of alveolar echinococcosis International conference

    Sako Y Y., Tappe D., Fukuda K., Kobayashi Y., Itoh S., Frosch M., Gruner B., Kern P., Ito A.

    XXIV World Congress of Hydatidology 

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    Event date： 2011.9

    Language：English   Presentation type：Poster presentation  

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  • 有鉤嚢虫症血清診断抗原の簡便な精製法の確立

    Sako Y., Okamoto M., Nakaya K., Ito A.

    日本寄生虫学会総会 

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    Event date： 2011.7

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • An immunochromatographic test for diagnosis of alveolar echinococcosis International conference

    Sako Y., Fukuda K., Kobayashi Y., Ito A.

    45th Annual Japan-USA Joint Conference on Parasitic Diseases 

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    Event date： 2011.1

    Language：English   Presentation type：Oral presentation (general)  

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  • Recent advances in immuno- and molecular-diagnosis of taeniasis and cysticercosis International conference

    Sako Y., Nkouawa A., Ito A.

    Joint International Tropical Medicine Meeting 2010 

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    Event date： 2010.12

    Language：English   Presentation type：Oral presentation (general)  

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  • 多包虫カテプシンＢ様システインペプチダーゼの性状解析

    迫 康仁, 中尾 稔, 中谷和宏, 伊藤 亮

    日本寄生虫学会総会 

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    Event date： 2009.3

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Molecular and Biochemical Approaches for Echinococcosis International conference

    Sako Y., Nakaya K., Nakao M., Ito A.

    XVIIth International Congress for Tropical Medicine and Malaria 

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    Event date： 2008.9 - 2008.10

    Language：English   Presentation type：Oral presentation (general)  

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  • 多包虫カテプシンＢ様システインペプチダーゼの遺伝子クローニング

    迫 康仁, 中尾 稔, 中谷和宏, 伊藤 亮

    日本寄生虫学会総会 

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    Event date： 2008.4

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 多包虫メタロプロテアーゼの遺伝子クローニング

    迫 康仁, 中谷和宏, 山崎 浩, 中尾 稔, 伊藤 亮

    日本寄生虫学会総会 

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    Event date： 2007.3

    Language：Japanese   Presentation type：Poster presentation  

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  • 多包虫カテプシンL様システインプロテアーゼの性状解析

    迫 康仁, 山﨑 浩, 中尾 稔, 中谷和宏, 伊藤 亮

    日本寄生虫学会総会 

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    Event date： 2006.5

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Recombinant Antigens of Serodiagnosis of Cysticercosis and Echinococcosis International conference

    Sako Y., Nakao M., Nakaya K., Yamasaki H., Ito A.

    Taeniasis/Cysticercosis and Echinococcosis International Symposium with Focus on Asia and the Pacific 

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    Event date： 2005.7

    Language：English   Presentation type：Oral presentation (general)  

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  • 多包虫カテプシンL様システインプロテアーゼの遺伝子クローニング

    迫 康仁, 山崎 浩, 中尾 稔, 中谷和宏, 伊藤 亮

    日本寄生虫学会総会 

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    Event date： 2005.4

    Language：Japanese   Presentation type：Oral presentation (general)  

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▼display all

Industrial property rights

  • 嚢虫症診断用抗原の精製方法（日本）

    迫 康仁, 伊藤 亮

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    Application no：特願2009-96360  Date applied：2009.4

    Announcement no：特開2010-248087A  Date announced：2010.11

    Patent/Registration no：特許第5582521号  Date registered：2014.7 

    Country of applicant：Domestic  

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Awards

  • 第15回日本寄生虫学会奨励賞

    2006.5   日本寄生虫学会  

    迫 康仁

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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Research Projects

  • Toward community-wide participation: Developing a non-invasive urine-based antibody test for schistosomiasis japonica

    Grant number：25K22729  2025.6 - 2028.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

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    Grant amount：\6,500,000 （ Direct Cost: \5,000,000 、 Indirect Cost：\1,500,000 ）

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  • Infection dynamics of the Schistosoma japonicum using a high-precision environmental analysis approach.

    Grant number：24KK0176  2024.9 - 2027.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Fund for the Promotion of Joint International Research(International Collaborative Research)

    Marcello Otake Sato, Yasuhiko Sako, Megumi Sato, Akihide Koyama, Emilie Takahashi

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    Grant amount：\20,800,000 （ Direct Cost: \16,000,000 、 Indirect Cost：\4,800,000 ）

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  • 寄生虫症の迅速で高精度な網羅的種鑑別診断法の開発研究

    2024.4 - 2027.3

    基盤研究(C)

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    寄生虫症を正確に診断し病因寄生虫を鑑別することは、「適切で迅速な治療」と「流行状況の把握」のために非常に重要である。しかし、現在の日本では、寄生虫症の正確な診断を行える医療機関は決して多くはないため、適切な治療を開始するまでに時間を要し、また世界的には寄生虫症の正確な診断による流行状況の把握ができていないため、効果的な寄生虫症の予防対策が滞っている。本研究の目的は「寄生虫検査の経験のない人でも迅速かつ簡便に、寄生虫の種を網羅的に鑑別診断でき、かつ社会実装できる高精度なpoint-of-care（POC）診断法」を開発することである。具体的には、ポータブルPCR装置を使用し、さらにポータブル次世代シーケンサーを組み合わせることにより、臨床現場や流行地で使用できる寄生虫の網羅的な種鑑別診断法を開発する。

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  • メコン住血吸虫の撲滅に向けた多面的な融合対策プログラム作成と数理モデルによる感染リスク分析による「最後の一押し！」

    2024.1 - 2027.3

    AMED 

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  • 国内に蔓延する寄生虫症、輸入寄生虫症の対策に資する研究開発

    2023.4 - 2026.3

    AMED 

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  • アジアの住血吸虫症撲滅を目指した革新的ポイント・オブ・ケア検査法ならびに環境汚染監視ツールの開発

    2022.1 - 2025.3

    AMED 

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  • Elucidating transmission dynamics for soil-transmitted helminths based on environmental DNA analysis

    Grant number：21K12269  2021.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\3,900,000 （ Direct Cost: \3,000,000 、 Indirect Cost：\900,000 ）

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  • Development of an innovative differential diagnosis method for diphyllobothriasis and taeniasis

    Grant number：20K08816  2020.4 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4,290,000 （ Direct Cost: \3,300,000 、 Indirect Cost：\990,000 ）

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  • Development of the detection method for animal cysticercosis and the investigation of the genetic factors for host specificity

    Grant number：15H05261  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Yanagida Tetsuya

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    Grant amount：\16,900,000 （ Direct Cost: \13,000,000 、 Indirect Cost：\3,900,000 ）

    In this study, it was aimed to develop the detection method for cysticercosis, caused by Taenia saginata or Taenia asiatica, in cattle and pigs. Field surveys in the endemic areas were conducted using the developed serology, and it revealed the method can detect T. saginata cysticercosis in cattle. Further extensive surveys are needed to examine the sensitivity and specificity of the method, and whether the method can detect T. asiatica cysticercosis in pig.
    It was for the first time directly demonstrated that cattle can be the intermediate host for the hybrid descendant between T. saginata and T. asiatica. This study indicates the possibility that comparative genomics can reveal the genetic factors controlling the host specificity of T. saginata and T. asiatica.

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  • Epidemiological survey of human taeniasis/cysticercosis and animal cysticercosis and development of comprehensive detection method for soil-transmitted helminths.

    Grant number：15H05273  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Sako Yasuhito

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    Grant amount：\15,990,000 （ Direct Cost: \12,300,000 、 Indirect Cost：\3,690,000 ）

    Field survey of human taeniasis/cysticercosis and animal cysticercosis has been conducted in Bali, Indonesia. In human survey we could not find cysticercosis sero-positive case but could find six Taenia solium taeniasis patients. In animal survey, we found that pigs had been exposed to Taenia hydatigena infection. Additionally, we demonstrated that the antigen quality for the detection of anti-Taenia adult antibody of taeniasis patient was affected by the taenia adult worm used for antigen preparation. Furthermore, we developed a simple DNA detection test to differentiate human taenia parasites by loop-mediated isothermal amplification in combination with dot enzyme-linked immunosorbent assay.

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  • Studies on proteinases of Echinococcus multilocularis larva for their survival in the host and search for inhibitors

    Grant number：15K08440  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Sako Yasuhito, OKAMOTO Munehiro, SASAKI Mizuki

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    Grant amount：\4,810,000 （ Direct Cost: \3,700,000 、 Indirect Cost：\1,110,000 ）

    We analyzed proteinases of Echinococcus multilocularis larva as key molecules on their persistent infection. Large scale preparation of active recombinant enzymes of four cathepsin-like cystein proteinases reported previously by us was not succeeded. However, we identified novel cathepsin L-like cystein proteinase (EmCLP3) and matrix metalloproteinase (EmMMP). We confirmed that both enzyme was expressed at protein level in larval stage. Furthermore, we demonstrated that EmCLP3 had an activity to digest host protein molecules such as IgG, albumin, collagen and fibrobnectin. These results indicated that EmCLP3 involved in the persistent infection of parasite.

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  • 有鉤条虫の比較ゲノム解析に基づく組織指向性の解明および遊離型ＤＮＡ検査法の開発

    Grant number：14F04103  2014.4 - 2016.3

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    迫 康仁, NKOUAWA AGATHE, NKOUAWA Agathe

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    Grant amount：\2,300,000 （ Direct Cost: \2,300,000 ）

    有鉤嚢虫症はテニア科条虫に属する有鉤条虫の幼虫の寄生に起因し、時に脳に寄生することにより致死的病態を引き起こす。有鉤嚢虫症は、ラテンアメリカでは脳内寄生のみによるものが大多数であるのに対し、アジアでは、脳内と他組織（主に皮下、筋肉）への寄生によるものが通常である。このような病態の差異を生じさせる組織指向性に関して、なぜアメリカ分離株とアジア分離株間で異なっているのか明確な解答は得られていない。そこで、ゲノムの塩基配列を株間で比較することにより、組織指向性を規定する因子の探索を試みた。同時に、血清中の寄生虫由来DNA検出法の確立を試みた。
    有鉤条虫のゲノム塩基配列を決定するために、タイ分離株では約12G、インドネシア分離株では約10Gの塩基配列データを取得することが出来た。アセンブルソフトAbyssならびにVelvetを用いてアセンブルを実施した結果、タイ分離株においては、Abyssでは最長鎖長820,191bp、velvetでは最長鎖長479,322bpの塩基配列が、インドネシア分離株においてはAbyssでは最長鎖長820,084bp、velvetでは最長鎖長479,322bpの塩基配列がアセンブルされた。今回得られた鎖長は短いため、全塩基配列を得るためにはさらなる塩基配列の取得が必要と考えられた。
    有鉤嚢虫感染ブタの血清虫に寄生虫由来のDNAが存在するか否かを確認する必要があるが、有鉤嚢虫感染ブタ血清を日本で調整することはきわめて困難である。そこで、有鉤嚢虫と同様に血管外寄生する条虫である多包虫を用いて、血管外寄生虫由来DNAの血清からの検出を試みた。多包虫が腹腔内に寄生しているマウスから血液を経時的に採取し、血清中の多包虫由来DNAの有無をPCR法にて確認した。その結果、PCRを2回実施することにより、マウス血清からPCR産物を得ることが出来た。

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  • Molecular evolution and co-evolution in Taeniid cestode infections in the world

    Grant number：24256002  2012.4 - 2015.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    ITO Akira, SAKO Yasuhito, NAKAO Minoru, NAKAYA Kazuhiro, YANAGIDA Tetsuya, OKAMOTO Munehiro, OKAMOTO Yoshiharu

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    Grant amount：\44,070,000 （ Direct Cost: \33,900,000 、 Indirect Cost：\10,170,000 ）

    The purpose of this project is better understanding of the evolutional aspect of pathogenic Taeniid cestodes infections in the world. All four genotypes of Echinococcus multilocularis have been exclusively confirmed from Russia. Mitochondrial DNA analysis of E. granulosus s. s. (G1) revealed that polymorphism of the gene was prominent in the specimens from the Middle East countries > China > Peru, and provided a hypothesis “The ancestral E. granulosus s.s. emerged in the Middle East”. Another purpose is better understanding of fertilization mechanisms in cestodes. Comparative studies of mitochondrial DNA (1N) and nuclear DNA (2N) genes using the sister species, Taenia saginata and T. asiatica, and the two (Asian and Afro/American) genotypes of T. solium sampled from areas where these species and two genotypes were sympatrically distributed revealed different types of hybrids. So, it is clear that out-crossing occurs in hermaphroditic cestode infections if two or more worms mature.

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  • Development of the quick diagnostic method for identifying Taenia asiatica and Taenia saginata for food safety and analysis of the gene causing the host specificity.

    Grant number：24406011  2012.4 - 2015.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    OKAMOTO Munehiro, SAKO Yasuhito, YANAGIDA Tetsuya, OKAMOTO Yoshiharu, MATSUI Atsushi

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    Grant amount：\17,290,000 （ Direct Cost: \13,300,000 、 Indirect Cost：\3,990,000 ）

    Taenia tapeworms collected from humans were examined for the mitochondrial cox1 gene and two nuclear genes, ef1, elp and pold. Phylogenetic analysis revealed that Taenia saginata and Taenia asiatica were once separated into two distinct taxa. On the other hand, some worms showed nuclear-mitochondrial discordance. Those facts indicate that they originated from hybridization between T. saginata and T. asiatica.

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  • 中国で蔓延している人獣共通寄生虫症(嚢虫症)の迅速・簡便な検査法の開発

    2012.4 - 2015.3

    日中医学協会 

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    嚢虫症の迅速かつ簡便な血清検査法の開発

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  • LAMP法によるエキノコックス条虫の迅速検査法の開発および感染源対策への応用

    2012.4 - 2014.3

    財団法人 大山健康財団 

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    エキノコックス条虫のLAMP法を用いた検出法の開発

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  • 難治性寄生虫病に関する遺伝子診断法の開発

    2010.8 - 2011.3

    文部科学省科学技術・学術政策局 

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  • 多包虫症に関する分子、免疫、伝搬生態学的研究

    2010.4 - 2012.3

    独立行政法人日本学術振興会 

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  • アジア・アフリカで流行している人畜共通寄生虫病研究拠点形成（Ⅱ）

    2009.4 - 2010.3

    独立行政法人日本学術振興会 

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  • Speciation of Taeniid cestodes (Echinococcus spp. and Taenia spp.) in the world and co-evolution of the host-parasite relationship

    Grant number：21256003  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    ITO Akira, NAKAO Minoru, NAKAYA Kazuhiro, SAKO Yasuhito, YANAGIDA Tetsuya, OKAMOTO Munehiro

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    Grant amount：\38,610,000 （ Direct Cost: \29,700,000 、 Indirect Cost：\8,910,000 ）

    Parasitic cestodes, Cestoda, Plathelminthes, have well been known to be hermatophroditic. Self-fertilization is the basic strategy for reproduction biology in cestodes. However, through our most recent studies on mitochondrial (haploid) and nuclear (diploid) DNA of two independent species infecting humans, Taenia saginata and Taenia asiatica have revealed that there are hybrids or hybrid derived adult tapeworms in Thailand and China where these species are sympatrically distributed. These data strongly reveal cross fertilization in the definitive host, humans, and launch “What is T. asiatica? Is it really independent species?" It is still under debates. Similar evidence showing cross fertilization between the intraspecies two, Asian and Afro/American genotypes of Taenia solium has been confirmed from larval stage of this parasite sampled in Madagascar. In Madagascar, we could have confirmed that the two genotypes are sympatrically distributed and also some hybrid individuals have been confirmed. Our work is the first one comparing both mitochondrial and nuclear genes in cestodes or platyhelminthes. These all data strongly suggest the cross fertilization in cestode reproduction biology. Molecular studies on Echinococcus spp. have also revealed that E. granulosus sensu lato is not a single species but 5 species, and a total of 9 independent species are included in the genus Echinococcus. Among these species, E. multilocularis is highly pathogenic and 3 (North American, Asian and European) genotypes have been known, but we have confirmed additional one, Mongolian genotype. The new information on the cestode speciation and identification has successfully been confirmed by the establishment of immuno- and molecular-diagnostic tools developed by our research team.

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  • Development of diagnostic methods for domestic animals infected with larval taeniid cestodes and application to risk management.

    Grant number：21406009  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    OKAMOTO Munehiro, ITO Akira, ITO Akira, OKAMOTO Yoshiharu

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    Grant amount：\17,550,000 （ Direct Cost: \13,500,000 、 Indirect Cost：\4,050,000 ）

    Parasitological survey on human taeniid cestods was don in Tibetan Plateau, Sichuan, China and Bali, Indonesia. In Tibetan Plateau, three human Taenia, Taenia solium, Taenia saginata and Taenia asiatica were endemic sympatorically. In Bali, T. solium was endemic in the northern part of island a d T. saginata was endemic in the southern part. In addition, hybrid between T. saginata and T. asiatica was found in Tibetan Plateau. We examined the molecul ar and immunological diagnostic methods for human taniasis/cysticercosis, which were developed by our research group. We also evaluated the risk of domestic animals for endemic of human taniasis/cysticercosis.

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  • 北海道特有の地方病（特にエキノコックス症等）に関する総合研究及び臨床応用

    2007.8 - 2010.3

    文部科学省研究振興局 

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  • アジア・アフリカで流行している人畜共通寄生虫病研究拠点形成

    2007.4 - 2009.3

    独立行政法人日本学術振興会 

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  • エキノコックス症における寄生虫の発育分化と宿主免疫応答の動的解析

    Grant number：19041009  2007 - 2008

    日本学術振興会  科学研究費助成事業  特定領域研究

    伊藤 亮, 中谷 和宏, 中尾 稔, 迫 康仁, 柳田 哲矢

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    Grant amount：\7,900,000 （ Direct Cost: \7,900,000 ）

    エキノコックス症(多包虫症、単包虫症)における1)宿主の免疫応答性解析ならびに2)寄生虫の遺伝子解析、3)新種Echinoccus shiquicusと他のエキノコックスの発育動態比較観察を中心に研究を実施した。1)WHOは多包虫症、単包虫症の病態をそれぞれPNMstage I〜IV, CL〜CE5と定義している。病態が正確に把握されている多数の多包虫症血清、単包虫症血清についてドイツのPKern教授、イタリアのE Brunetti教授の研究グループからそれぞれ提供を受けた。エキノコックス症感染動態とTotal IgGの動態解析を実施し、それぞれ共著論文を作成し、投稿した。RecEm18-ELISAが多包虫症の感染動態を正確に評価できる抗体指標になること、RecAgB8/1-ELISAが単包虫症の感染動態、特に活性病巣を有している症例のほぼ90%を検出できることが判明した。現在は、IgG subclass, IgE応答を解析中である。2)ミトコンドリアならびに核遺伝子を用いるエキノコックス属条虫種の再評価を実施し、アフリカライオンに寄生している種を独立種(Echinococcus felidis)として再記載した。ヨーロッパ、南米、中国に分布しているいわゆる単包条虫(E. granulosus sensu strict)ならびに北半球に分布している多包条虫(E. multilocularis)、中国チベット高原から発見された新種エキノコックス条虫(E. shiquicus)の遺伝子多型を解析し、E. shiquicusが遺伝子多型に富んでいるのに対し、他の2種では地域を問わずほぼ均一の遺伝子型が中心になっていることが判明した。3)E. shiquicus幼虫の形態は他の条虫と大きく異なっていることが判明した。中国に分布しているエキノコックス条虫3種の発育様式の比較、公衆衛生学的に重要な多包虫症、単包虫症の病態と抗体応答のさらなる解析が今後の課題である。

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  • In situ localization of cysteine proteinase of Taenia solium cysticercus

    Grant number：19590423  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4,550,000 （ Direct Cost: \3,500,000 、 Indirect Cost：\1,050,000 ）

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  • Molecular epidemiological survey and risk evaluation of livestock animals for control of taeniasis and cycticercosi s.

    Grant number：18406008  2006 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    OKAMOTO Munwhieo, OKAMOTO Yoshiharu, ITO Akira, SAKO Yasuhito

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    Grant amount：\15,750,000 （ Direct Cost: \13,200,000 、 Indirect Cost：\2,550,000 ）

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  • エキノコックス症化学療法剤の標的分子候補としてのシステインプロテアーゼの解析

    Grant number：18790289  2006 - 2007

    日本学術振興会  科学研究費助成事業  若手研究(B)

    迫 康仁

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    Grant amount：\3,400,000 （ Direct Cost: \3,400,000 ）

    エキノコックス幼虫システインプロテアーゼを標的分子とする化学療法剤の開発のために必須である、その一次構造を決定し、性状の解析を試みた。本年度は、エキノコックス幼虫カテプシンB様システインプロテアーゼ(EmCtB)に関する解析を行った。
    1.カテプシンB様システインプロテアーゼ遺伝子のクローニング
    システインプロテアーゼの酵素活性部位に高度に保存されたアミノ酸配列を基にプライマーを設計し、エキノコックス幼虫cDNAを鋳型としPCRを行い、システインプロテアーゼ遺伝子の一部分をクローニングした。既にクローニングしてあるシステインプロテアーゼ遺伝子をDNAハイブリダイゼーション法により除去することにより、新規遺伝子をクローニングした。DNA解析の結果、得られたクローンは、カテプシンB様システインプロテアーゼに相同性を示した。また、この遺伝子をEmCtBと命名した。
    2.エキノコックス幼虫での発現解析
    EmCtBに対するモノクローナル抗体を調整し、エキノコックス幼虫抽出抗原および分泌・排泄(ES)液に対してイムノブロット解析を行った結果、抽出抗原およびES抗原で25.6および26.4kDaの蛋白質が検出された。これらの結果より、両酵素が蛋白質レベルで発現していること、また、一部が分泌されていることが明らかとなった。次に、エキノコックス幼虫での局在を解析するために、免疫組織染色を行った。その結果、胚層、繁殖胞および原頭節で発現していることが確認できた。
    3.活性型EmCtBの発現
    活性型酵素を酵母(Pichia pastoris)を用いて発現させた結果、酵素活性を持つ十分量の組換え蛋白質を得ることが出来た。また、組換え酵素は高度に糖付加を受けている事が明らかとなった。

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  • Speciation, pathogenicity and evolution of Echinococcus and Taenia tapeworms in Asia

    Grant number：17256002  2005 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    ITO Akira, NAKAO Minoru, NAKAYA Kazuhiro, SAKO Yasuhito, YANAGIDA Tetsuya, OKAMOTO Munenori

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    Grant amount：\41,860,000 （ Direct Cost: \32,200,000 、 Indirect Cost：\9,660,000 ）

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  • Severe Combined Immunodeficient(SCID) Mice As Animal Models for Human Echinococcosis

    Grant number：15500295  2003 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    NAKAYA Kazuhiro, YAMASAKI Hiroshi, NAKAO Minoru, SAKO Yasuhito, ITO Akira

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    Grant amount：\3,800,000 （ Direct Cost: \3,800,000 ）

    At first, two strains of sever combined immune-deficiency mice, C.B-17/Icr-scid and NOD/Shi-scid were compared with the sensitivity to Echinococcus multilocularis metacestode. At post infection 9 and 12weeks, the developed metacestode of two strains infra peritoneal cavities were weighed and compared. NOD/Shi-scid showed more high degree weight than C.B-17/Icr-scid at both points of weeks. It was suggested that scid-gene, declines of macrophage function and compliment activity were influenced together.
    So, development and growth of metacestode in NOD/Shi-scid were observed in detail. At post infection 1,2,3 weeks, between NOD/Shi-scid and control NOD/Shi-+/+ mice, there were no defferences both weight and morphologies of metacestodes. NOD/Shi-+/+ mice were not revealed for rEm-18 antibody in these periods. It was considered that so-called prepatent period which antibody was not yet producted in host immune system. After post infection 6 weeks, in NOD/Shi-scid mice, multivesiculation and formation of protoscolex were rapidly proceeded. NOD/Shi-scid mice showed striking torelance for the parasite development and growth. It was suggested that NOD/Shi-scid mouse would be a model for human echinococcosis.
    And moreover, it was attempted of quantification of multivesiculation and protoscolex formation of the parasite between NOD/Shi-scid and NOD/Shi-+/+ mice. After post infection 6 weeks, in NOD/Shi-scid mice, index of multivesiculation and protoscolex formation suddenly increased. While in NOD/Shi-+/+ mice, in spite of spread of parasite and inflamated fucus, index of multivesicelation was decreased for not changed number of vesicular. Index of protoscolex also increased more in NOD/Shi-scid mice than in NOD/Shi-+/+ mice. It was expected that index methods of these parasite development or growth was usefullness in evaluation of host sensitivity and drug efficacy, estimate of infectious point.

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  • Immunodiagnosis for echinococcosis: international standardization, early diagnosis and evaluation of prognosis

    Grant number：12557024  2000 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    ITO Akira, YAMASAKI Hiroshi, NAKAYA Kazuhiro, KOGO Yutaka, NAKAO Minoru, FUJIMOTO Yoshinori

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    Grant amount：\13,300,000 （ Direct Cost: \13,300,000 ）

    The main results from this project to identify patients of alveolar echinococcosis (AE) serologically are as followings.
    1. The establishment of a new serodiagnosis for identification of alveolar echinococcosis using a recombinant Em18 (RecEm18) (Sako et al. 2002. Journal of Clinical Microbiology 40, 2769-2765) and affinity-purified Em18 (Xiao et al. submitted for publication) and demonstration that Em18 is the product of degradation of EM10 by cysteine proteinase and has the least homology with human EMR proteins and be expected to have the highest B cell epitope activity among all other candidate protein antigens.
    2. The usefulness of RecEm18 has been evaluated as a blind test with French group (Ito et al. 2002. Journal of Clinical Microbiology 40, 4161-4165). Additional work to confirm AE using RecEm18 has just started with French and Swiss groups.
    3. Serological differentiation of another echinococcosis, cystic echinococcosis (CE) has also been well advanced (Mamuti et al. 2002. Clinical and Diagnostic Laboratory Immunology 9, 573-576). Production of a recombinant Antigen B (RecAgB) has been succeeded (Mamuti et al. in preparation).
    4. Serology to identify neurocysticercosis (NCC) of Taenia solium has been established using a recombinant chimeric antigen (Sako et al. 2000. Journal of Clinical Microbiology 38, 4439-4444).
    5. These serological methods to identify AE, CE and NCC are the best in the world and have been applied for international collaboration projects and have been reviewed in several journals and books.
    6. Molecular work to differentiate Echinococcus species and Taenia species has been established.
    7. We have published 29 original papers, 14 proceedings and 5 chapters in three books in English last three years. To establish rapid system for these serodiagnosis and molecular diagnosis is the next step of our research.

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  • 分子生物学的アプローチによる難治性寄生虫感染症の血清診断法の開発

    Grant number：12770122  2000 - 2001

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    迫 康仁

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    Grant amount：\2,000,000 （ Direct Cost: \2,000,000 ）

    新興・再興感染症として世界規模で流行し汚染地域拡大が年々深刻化している人体寄生条虫症の1つである有鉤嚢虫症の高品質な診断用抗原の安定供給とそれを用いた血清診断法の国際標準化を最終的な目的としている。本研究では、前年度に確立した組換え抗原を用いた血清診断法の評価を行うとともに、分離株間の遺伝子多型について解析を行った。
    1)組換え蛋白質を用いた有鉤嚢虫血清診断法の評価
    組換え抗原は、有鉤嚢虫症に対する特異性は十分なものの、感度の点で問題があった。すなわち、有鉤嚢虫の多数寄生の場合は9割近くの特異抗体を検出することができたが、1個体寄生の場合、有鉤嚢虫液より精製した抗原分子を用いた診断では9割近くを陽性と判断できるのに対し、組換え蛋白質では7割前後までにその感度が減少した。抗原蛋白質をコードする遺伝子は多重遺伝子ファミリーを形成していることが明らかとなっているため、更なる遺伝子のクローニングの必要性が示唆された。また、本抗原蛋白質は高度に糖鎖修飾を受けている分子であることから、有鉤嚢虫特異的な糖鎖構造の抗原としての重要性も示唆された。
    2)分離株間の抗原遺伝子多型
    分離株(中国、インドネシア、エクアドル、ペルー)ゲノムDNAより抗原遺伝子をPCRにより増幅し、その遺伝子構造および遺伝子多型について解析した。その結果、抗原遺伝子は3つのエクソンと2つのイントロンを有していた。また、分離株間で極めて高い相同性(98%)を示した。これより、組換え蛋白質を用いた血清診断において熟慮しなければならない分離株間の抗原性の多様性を克服することが出来得ると示唆された。

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  • SERO-AND MOLECULAR-EPIDEMIOLOGICAL STUDY ON ECHINOCOCCOSIS AND CYSTICERCOSIS IN ASIA (2000)

    Grant number：11694259  1999 - 2000

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A).

    ITO Akira, OKAMOTO Munehiro, HORII Toshihiro, KANAZAWA Tamotsu, SAKO Yasuhito, NAKAO Minoru

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    Grant amount：\11,500,000 （ Direct Cost: \11,500,000 ）

    Zoonotic larval cestode infections involving echinococcosis and cysticercosis are threatening human life and involved in Emerging/Re-emerging infectious diseases worldwide and the endemic areas are spreading year by year. Echinococcosis has been involved on the category 4 in the new Prevention of Infectious Diseases in Japan from April 1999. However, there is no reliable differential serodiagnosis without surgical confirmation. It is too behind of modern science and technology. My research group members at Asahikawa Medical College (AMC) has already established highly reliable serodiagnoses for differentiation of these three serious parasitic diseases. The serodiagnoses at AMC has been evaluated to be the top level (one of the top two) in the world. There is no false positive case for alveolar echinococcosis (AE) from other diseases at all and it is sound for neurocysticercosis (NCC) too. Therefore, many research groups in the world has contacted with AMC including WHO.Recombinant antigens for serodiagnosis of AE and NCC have been produced at AMC.AMC group has succeeded in the analysis of full length of mitochondrial DNA of Echinococcus multilocularis and Taenia solium, the causative pathogens for AE and NCC, respectively. Therefore, it is expected to establish DNA diagnosis for differentiation of any taeniid cestodes of human origin. AMC group members are now involved in many international collaboration projects on the control of echinococcosis and neurocysticercosis not only in Asia but also in the world. AMC group is expecting to establish the research and reference centers for serodiagnosis and molecular diagnosis for these zoonotic cestodiases in Asia.

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  • MOLECULAR- AND IMMUNO-DIAGNOSIS FOR EMERGING/RE-EMERGING INFECTIOUS DISEASES, ECHINOCOCCOSIS AND CYSTICERCOSIS (1999)

    Grant number：10557029  1998 - 1999

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    ITO Akira, NAKAO Minoru, OKAMOTO Munehiro, HORII Toshihiro, SAKO Yasuhito

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    Grant amount：\12,300,000 （ Direct Cost: \12,300,000 ）

    Echinococcosis and cysticercosis are most serious zoonotic cestode infections worldwide. The endemic areas are spreading year by year almost all over the world. As these diseases are threatening human life and immuno- and molecular-diagnosis for these diseases are not easy so far examined, we have started to establish better resolution for differential immuno- and molecular-diagnosis as international collaboration projects from 1994. The present research results are highly promising but they are joined good results from the international collaboration projects sponsored by the Ministry of Education, Science, Sports and Culture (11694259). We developed highly reliable serodiagnosis for differentiation of alveolar echinococcosis, cystic echinococcosis and neurocysticercosis using native antigens. Based on huge number of important patients' sera from almost all over the world through WHO informal echinococcosis working group members and others, our serodiagnosis is now evaluated to have the top quality in specificity and sensitivity in the world. The invited paper entitled "Differential serodiagnosis for larval cestode infections: cystic echinococcosis, alveolar echinococcosis and neurocysticercosis" presented by Akira Ito at the 33rd International Congress of Hydatidology held in Argentina September 1999 was nominated as the best paper on echinococcosis in 1999. We further have established recombinant antigens for neurocysticercosis and now are planning to produce recombinant antigens highly useful for alveolar echinococcosis and cystic echinococcosis from 2000 if we can get additional research grant money. We have also analyzed the full length of mitochondrial DNA of Echinococcus multilocularis and Taenia solium. As it is the first success in the world, we have been asked to collaborate for molecular epidemiology of these larval cestode infections in the world.

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  • SEVERE COMBINED IMMUNODEFICIENT (SCID) MICE AS ANIMAL MODELS FOR HUMAN CYSTICERCOSIS AND ECHINOCOCCOSIS (1999)

    Grant number：10480235  1998 - 1999

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    ITO Akira, SAKO Yasuhito, NAKAO Minoru, ITO Mamoru, NAKAYA Kazuhiro

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    Grant amount：\10,500,000 （ Direct Cost: \10,500,000 ）

    Zoonotic larval cestode infections involving cysticercosis and echinococcosis are involved in Emerging/Re-emerging infectious diseases worldwide. As human taeniid tapeworm infections, we recognize three species : Taenia solium, T. saginata and T. asiatica (= T. sagainata asiatica). As (1) these taeniid cestodes require such domestic animals as cattle and pigs and even humans as the intemediate host and (2) the definitive host is only humans, it is very difficult to analyze the mechanism of the host-parasite relationship. We tried to establish a net-work system for getting viable eggs from any countries in the world at first, and when we could get eggs, we used immunodeficient mice including severe combined inununodeficient (scid) mice, congenitally athymic nude mice, and knock-out mice for experimental infections of taeniid eggs. We found that nonobese diabetic (NOD)-scid mice of both sexes are supreme experimental animal models for cysticercosis of human taeniid cestode infections. It was concluded that four-month-old cysticerci of T. saginata, developed in a NOD-scid mouse were mature ones, since when a volunteer ingested three cysticerci, he got infection. Another topic of our research was that we found unexpected basic life cycle of cestodes in NOD-scid and NMRI-nude mice. Hymenolepis microstoma requires beetles as obligatory intermediate hosts. However, when NMRI-nude and NOD-scid mice were orally given 5 cysticercoids, they harbored many adult tapeworms over the five original ones. We confirmed that oncospheres of H. microstoma could invade the intestinal tissue of these immunodeficient mice and develop into cysticercoids within 11 days. Cysticercoids of H. microstoma remained tailed, beetle-derived ones in these mice, although H. nana, only exceptional cestode to have a direct life cycle in a mouse, develops tailless, mouse-derived one in a mouse. It is now easy for us to analyze these two very important basic researches on immunobiology of cestode infections using NOD-sicd mice.

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  • Development of Serodiagnosis of Parasite Infection

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    Grant type：Competitive

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  • エキノコックス症血清検査法の開発 International coauthorship

    （選択しない） 

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    難治性寄生虫症である多包虫および単包虫症の組換え抗原を用いた、感度の高い特異抗体検出法の開発

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  • 有鉤嚢虫症血清検査法の開発 International coauthorship

    （選択しない） 

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    致死性寄生虫疾患である有鉤嚢虫症の、組換え抗原を用いた感度の高い特異抗体検出法の開発

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  • 条虫-宿主の分子間クロストークの解明

    （選択しない） 

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    条虫幼虫の宿主での生存戦略としての分子間クローストークの解明。特に、膜蛋白質および分泌蛋白質の活性についての解析。

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  • 多包虫蛋白質分解酵素の解析

    （選択しない） 

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    多包虫の分子標的治療薬開発のための、寄生虫蛋白質分解酵素の性状解析

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  • 寄生虫感染症の血清診断法の開発

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    Grant type：Competitive

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  • 条虫DNA検出に基づく鑑別検査法の開発 International coauthorship

    （選択しない） 

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    マルチプレックスPCR法ならびにLAMP法を用いた条虫DNA検出に基づく条虫種鑑別検査法の開発。特に、糞便を検体とした検出法の開発。

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Social Activities

  • JICA 地域保健担当官のための保健行政研修 脳嚢虫症・エキノコックス症

    2015.7

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    平成27年7月1日（水） 旭川医科大学 アフリカ地域９カ国

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  • JICA 地域保健担当官のための保健行政 講義 脳嚢虫症・エキノコックス症

    2014.7

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    平成26年7月7日(月) 旭川医科大学 アフリカ地域 ８カ国

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  • 北海道感染症危機管理対策協議会 委員

    2014.1

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    平成２９年９月８日 会議出席
    平成３１年３月２７日 会議出席
    令和２年９月 メール会議

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