担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3389/fnana.2021.759804. eCollection 2021.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Trunk muscles in vertebrates are classified as either dorsal epaxial or ventral hypaxial muscles. Epaxial and hypaxial muscles are defined as muscles innervated by the dorsal and ventral rami of spinal nerves, respectively. Each cluster of spinal motor neurons passing through dorsal rami innervates epaxial muscles, whereas clusters traveling on the ventral rami innervate hypaxial muscles. Herein, we show that some motor neurons exhibiting molecular profiles for epaxial muscles follow a path in the ventral rami. Dorsal deep-shoulder muscles and some body wall muscles are defined as hypaxial due to innervation via the ventral rami, but a part of these ventral rami has the molecular profile of motor neurons that innervate epaxial muscles. Thus, the epaxial and hypaxial boundary cannot be determined simply by the ramification pattern of spinal nerves. We propose that, although muscle innervation occurs via the ventral rami, dorsal deep-shoulder muscles and some body wall muscles represent an intermediate group that lies between epaxial and hypaxial muscles.

DOI： 10.1111/joa.13219

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記述言語：日本語   出版者・発行元：(公社)日本顕微鏡学会  

切片SEM法は、新たな走査電子顕微鏡(SEM)イメージング技法であり、スライドガラスに貼り付けた樹脂包埋組織切片から反射電子(BSE)像を観察する手法である。この手法では、高度なミクロトーム技術を必要とすることなく、透過電子顕微鏡のような超薄切片像を取得することができる。そこで本稿では、私たちが独自に開発した3つの切片SEM法、1)準超薄切片から超薄像をイメージする手法(準超薄切片SEM法)、2)蛍光像と準超薄切片BSE像の相関顕微鏡観察法(CLEM法)、3)凍結薄切法(徳安法)との組み合わせ法、について紹介する。さらに、新たな3Dイメージング技法である「連続切片SEM法」についても紹介し、切片のSEMイメージング技術の可能性について考察する。(著者抄録)

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J04085&link_issn=&doc_id=20200528350003&doc_link_id=10.11410%2Fkenbikyo.55.1_18&url=https%3A%2F%2Fdoi.org%2F10.11410%2Fkenbikyo.55.1_18&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_2.gif

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1152/ajpheart.00108.2019

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The osmium maceration method is a powerful technique for observing the three-dimensional ultrastructure of cellular organelles by scanning electron microscopy. In the conventional osmium maceration method, tissues are immersed in a diluted osmium tetroxide solution for several days at 20°C to remove soluble cytosolic proteins from the freeze-cracked surface of cells, and the optimal duration of this process is dependent on the cell type. To improve the efficiency of the osmium maceration procedure, we have examined systematically the relationship between the reaction temperature and time of the osmium maceration procedure. Treatment at temperatures higher than 20°C drastically shortened the time required to remove cytosolic proteins from the freeze-cracked surface of specimens with optimal durations for the osmium maceration of hepatocytes at 30, 40, 50 and 60°C being 30, 15, 5 and 1 h, respectively. Considering the stability and reproducibility of the macerated specimens, we concluded that the most appropriate temperature was 30 to 40°C. This rapid osmium maceration procedure was used successfully to observe the 3D ultrastructure of Purkinje cells in the cerebellum and proximal convoluted tubule cells in the kidney. This simple and reproducible rapid osmium maceration protocol should find wide appeal for the 3D analysis of cellular organelles in various cell types.

DOI： 10.2220/biomedres.41.161

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

OBJECTIVES: Continuously growing rodent incisors have an apically located epithelial stem cell compartment, known as an "apical bud" (AB). Few studies have described the morphological features of ABs and stem cell niches in continuously growing premolars/molars. We attempted to clarify the relationship between the three-dimensional configuration of ABs and the stem cell niches in guinea pig cheek teeth. METHODS: We perfusion-fixed four-week-old guinea pigs, then decalcified their premolars/molars to produce serial paraffin sections, which we immunostained for Sox2. We reconstructed the serial sections using image processing and analysis software. We processed undecalcified samples for scanning electron microscopy by KOH digestion. RESULTS: Two types of epithelia with M and Δ shapes surrounded the S-shaped dental papilla in the apical region of the premolars/molars, and there were three Sox2-positive epithelial bulges above the M- and Δ-shaped epithelia. Sox2-positive epithelial stem cell niches were restricted to the apical side, and cell proliferation and differentiation immediately proceeded in the crown-analogue dentin. The Sox2-positive epithelial stem cell niches were sparsely distributed and extended to the occlusal side. We also detected continuously proliferating cells in the cervical loop and Hertwig's epithelial root sheath of the root-analogue dentin. CONCLUSIONS: Our findings suggest that guinea pig cheek teeth have three ABs, and the complex configuration of these types of teeth may be attributed to the prompt formation of crown-analogue dentin followed by the long-term formation of root-analogue dentin.

DOI： 10.1016/j.job.2019.01.002

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

DOI： 10.21820/23987073.2019.4.46

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1042/BCJ20180832

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Oxford University Press  

Secretogranin III (SgIII), a member of the granin family, binds bothtoanother granin, chromogranin A (CgA), and to a cholesterol-rich membrane that is destined for secretory granules (SGs). The knock down of Sg III in adrenocorticotropic hormone (ACTH)-producingAtT-20cellslargelyimpairs the regulated secretion of CgA and ACTH. To clarify the physiological roles of SgIII in vivo, we analyzed hormone secretion and SG biogenesis in newly established SgIII-knockout (KO) mice. Although the SgIII-KO mice were viable and fertile and exhibited no overt abnormalities under ordinary rearing conditions, a high-fat/high-sucrose diet caused pronounced obesity in the mice. Furthermore, in the SgIII-KO mice compared with wild-type (WT) mice, the stimulated secretionofactive insulin decreased substantially, whereas the storage of proinsulin increased in the islets. The plasma ACTH was also less elevated in the SgIII-KO mice than in the WT mice after chronic restraint stress, whereas the storage level of the precursor proopiomelanocortin in the pituitary gland was somewhat increased. These findings suggest that the lack of SgIII causes maladaptation of endocrine cells to an inadequate diet and stress by impairing the proteolytic conversion of prohormones in SGs, whereas SG biogenesis and the basal secretion of peptide hormones under ordinary conditions are ensured by the compensatory upregulation of other residual granins or factors.

DOI： 10.1210/en.2017-00636

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Biomedical Research Foundation  

We describe a novel immuno-scanning electron microscopy (SEM) technique that combines both Tokuyasu’s cryosectioning and section SEM methods. In this technique, semithin cryosections, cut according to the Tokuyasu method, were adhered to glass microscope slides, immunostained for bio-molecules of interest and observed by confocal laser scanning microscopy. The same sections were subsequently embedded in epoxy resin and ultrathin sections were cut on an ultramicrotome. These were then observed by SEM using a backscattered electron detector. Correlation between immunofluorescence and SEM images was performed in the same area of the cryosection. Immuno-SEM was also performed using a FluoroNanogold-labeled secondary antibody. This novel immuno-SEM method can provide ultrastructural information of cell organelles in relation to associated molecules, such as Golgi- and ER-associated proteins. This novel immuno-SEM technique has the potential to be widely used.

DOI： 10.2220/biomedres.39.21

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.2220/biomedres.39.197

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/jmicro/dfy028

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PUBLIC LIBRARY SCIENCE  

The effects of warm machine perfusion preservation of liver grafts donated after cardiac death on the intracellular three-dimensional ultrastructure of the organelles in hepatocytes remain unclear. Here we analyzed comparatively the ultrastructure of the endomembrane systems in porcine hepatocytes under warm ischemia and successive hypothermic and midthermic machine perfusion preservation, a type of the warm machine perfusion. Porcine liver grafts which had a warm ischemia time of 60 minutes were perfused for 4 hours with modified University of Wisconsin gluconate solution. Group A grafts were preserved with hypothermic machine perfusion preservation at 8 degrees C constantly for 4 hours. Group B grafts were preserved with rewarming up to 22 degrees C by warm machine perfusion preservation for 4 hours. An analysis of hepatocytes after 60 minutes of warm ischemia by scanning electron microscope revealed the appearance of abnormal vacuoles and invagination of mitochondria. In the hepatocytes preserved by subsequent hypothermic machine perfusion preservation, strongly swollen mitochondria were observed. In contrast, the warm machine perfusion preservation could preserve the functional appearance of mitochondria in hepatocytes. Furthermore, abundant vacuoles and membranous structures sequestrating cellular organelles like autophagic vacuoles were frequently observed in hepatocytes after warm machine perfusion preservation. In conclusion, the ultrastructure of the endomembrane systems in the hepatocytes of liver grafts changed in accordance with the temperature conditions of machine perfusion preservation. In addition, temperature condition of the machine perfusion preservation may also affect the condition of the hepatic graft attributed to autophagy systems, and consequently alleviate the damage of the hepatocytes.

DOI： 10.1371/journal.pone.0186352

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER IRELAND LTD  

In the Japanese Archipelago, Ezohelix gainesi, a member of bradybaenid land snails, is endemic mainly to the island of Hokkaido. During July to August of 2016, a survey to detect trematode infections from E. gainesi was carried out at a forest city park in Asahikawa, Hokkaido. Systemic infections of the snails with sporocysts containing short-tailed cercariae were found in 5.3% of 94 individuals examined. Furthermore, most of them (90.4%) harbored non-encysted metacercariae within their kidneys. A DNA sequence identification revealed that both of the sporocyst and the metacercaria belong to an unknown species of the family Brachylaimidae. The metacercariae showed a genetic diversity with 6 haplotypes of mitochondrial DNA (mtDNA) even in the limited sampling area. A definitive host of the unknown species could not be determined, although 34 field mice (Apodemus speciosus) and 21 voles (Myodes rufocanus) from the city park were examined for intestinal parasites. To examine the adult stage, the metacercariae were perorally administrated to mice, together with anti-inflammatory treatment with methylprednisolone. Fully matured adult worms were recovered from the intestinal ileum 8 and 14 days postinfection. The gravid adults showed typical features of the genus Brachylaima. A morphological and biogeographical evaluation prompted us to propose Brachylaima ezohelicis sp. nov. for the parasite from E. gainesi. The autochthony of the first intermediate host and the spatial heterogeneity of mtDNA suggest that the new species found in the city park is not a recently expanded population of immigrant origin. (C) 2017 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.parint.2017.01.015

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

The structure of the Golgi apparatus has been extensively examined by light and electron microscopy, but details of its three-dimensional (3D) structure have remained unclear because of the technical limitations of conventional microscopy techniques. To overcome this problem, we have developed several novel scanning electron microscopy (SEM) methods for observing the 3D structure of subcellular organelles including the Golgi apparatus: (1) an osmium maceration method that facilitates SEM observation of membranous organelles, including the Golgi apparatus, by selectively removing soluble cytoplasmic proteins, (2) an osmium impregnation/maceration method that combines an osmium impregnation method with the osmium maceration method to determine the polarity of the Golgi apparatus by SEM, (3) a correlative light and SEM method that combines a cryosectioning technique with the osmium maceration method to enable correlation of the immunocytochemical distribution of molecules with the 3D ultrastructure of the Golgi apparatus, and (4) array tomography based on the systematic collection and integration of SEM images of serial ultrathin sections on glass slides for revealing the 3D ultrastructure of the entire Golgi apparatus. Together, the novel SEM techniques listed above can reveal the complete 3D structure of the Golgi apparatus in different cell types.

DOI： 10.1007/s12565-016-0380-8

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BIOMEDICAL RESEARCH PRESS LTD  

The increased discharge of gonadotropin releasing hormone (GnRH) from hypothalamic neurons after castration specifically stimulates pituitary gonadotropes. To elucidate the putative effects of GnRH on the three-dimensional ultrastructure of gonadotropes, we examined osmium-macerated pituitary tissues of male rats at various time points after castration by high resolution scanning electron microscopy (SEM) combined with immunocytochemistry. Two days after castration, the Golgi apparatus was disassembled into small stacks; patch-like, tubuloreticular clusters of endoplasmic reticulum (ER) membranes were present; and spherically enlarged mitochondria were accumulated in the central area of the stimulated gonadotropes. These acute changes were indiscernible by 1 week after castration, and then the pituitary gonadotropes of castrated animals gradually became hypertrophic, finally exhibiting the characteristic "signet-ring" appearance, with markedly dilated cisterns of the rough ER. Upon SEM observation, the inner surface of the cavity was mostly flat, and openings connecting adjacent lumens of the ER were sparse. Proliferation of the osmiophilic tubular network of the ER-Golgi intermediate compartment was observed in the persistently stimulated gonadotropes, indicating a marked increase in trafficking of secretory proteins between the Golgi and ER. The acute and chronic changes in the gonadotropes after castration revealed in the present study by SEM provide evidence for a putative link between the intracellular signaling events evoked by GnRH and the ultrastructural dynamics of the organelles of the secretory pathway.

DOI： 10.2220/biomedres.38.1

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BIOMEDICAL RESEARCH PRESS LTD  

In the cochlea, a high K+ environment in the endolymph is essential for the maintenance of normal hearing function, and the transport of K+ ions through gap junctions of the cochlear epithelium is thought to play an important role in endolymphatic homeostasis. The aim of the present study was to demonstrate the three-dimensional (3D) ultrastructure of spiral ligament root cells and interdental cells, which are located at both ends of the gap junction system of the cochlea epithelium. Serial semi-thin sections of plastic-embedded rat cochlea were mounted on glass slides, stained with uranyl acetate and lead citrate, and observed by scanning electron microscopy (SEM) using the backscattered electron (BSE) mode. 3D reconstruction of BSE images of serial sections revealed that the root cells were linked together to form a branched structure like an elaborate "tree root" in the spiral ligament. The interdental cells were also connected to each other, forming a comb-shaped cellular network with a number of cellular strands in the spiral limbus. Furthermore, TEM studies of ultra-thin sections revealed the rich presence of gap junctions in both root cells and interdental cells. These findings suggest the possibility that both root cells and interdental cells contribute to K+ circulation as the end portion of the epithelial cell gap junction system of the cochlea.

DOI： 10.2220/biomedres.38.239

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BIOMEDICAL RESEARCH PRESS LTD  

Thiamine pyrophosphatase (TPPase) cytochemistry is an established method for specific labeling of the trans-Golgi cisterns in tissue sections. Herein, we combined this enzyme cytochemical method with array tomography using scanning electron microscopy (SEM), a new imaging technique based on collection of backscattered electron (BSE) images of consecutive resin-embedded sections on glass slides, to detect the entire three-dimensional (3D) organization of the Golgi apparatus with sufficient spatial resolution. As the signal intensity of BSE depends on the atomic number of the materials, lead precipitates confined to the trans-Golgi cisterns after TPPase cytochemistry were clearly observed by BSE-mode SEM. The mild fixative used for TPPase cytochemistry also enabled accurate identification of target gonadotropes in the composite pituitary tissue by immunocytochemical staining. By 3D reconstruction of the entire trans-Golgi cisterns based on serial ultrathin section images of tissues after TPPase cytochemistry, we detected ultrastructural differences in the 3D configuration of the Golgi apparatus between cerebellar Purkinje cells and pituitary gonadotropes. The appropriate combination of enzyme cytochemistry and/or immunostaining with array tomography will further clarify the relationship between the organization and functional states of the Golgi apparatus.

DOI： 10.2220/biomedres.38.285

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER JAPAN KK  

Lymphocyte recruitment into the portal tract is crucial not only for homeostatic immune surveillance but also for many liver diseases. However, the exact route of entry for lymphocytes into portal tract is still obscure. We investigated this question using a rat hepatic allograft rejection model.
A migration route was analyzed by immunohistological methods including a recently developed scanning electron microscopy method. Transmigration-associated molecules such as selectins, integrins, and chemokines and their receptors expressed by hepatic vessels and recruited T-cells were analyzed by immunohistochemistry and flow cytometry.
The immunoelectron microscopic analysis clearly showed CD8 beta(+) cells passing through the portal vein (PV) endothelia. Furthermore, the migrating pathway seemed to pass through the endothelial cell body. Local vascular cell adhesion molecule-1 (VCAM-1) expression was induced in PV endothelial cells from day 2 after liver transplantation. Although intercellular adhesion molecule-1 (ICAM-1) expression was also upregulated, it was restricted to sinusoidal endothelia. Recipient T-cells in the graft perfusate were CD25(+)CD44(+)ICAM-1(+)CXCR3(+)CCR5(-) and upregulated alpha 4 beta 1 or alpha L beta 2 integrins. Immunohistochemistry showed the expression of CXCL10 in donor MHCIIhigh cells in the portal tract as well as endothelial walls of PV.
We show for the first time direct evidence of T-cell transmigration across PV endothelial cells during hepatic allograft rejection. Interactions between VCAM-1 on endothelia and alpha 4 beta 1 integrin on recipient effector T-cells putatively play critical roles in adhesion and transmigration through endothelia. A chemokine axis of CXCL10 and CXCR3 also may be involved.

DOI： 10.1007/s00535-016-1169-1

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-BLACKWELL  

The osmium maceration method with scanning electron microscopy (SEM) enabled to demonstrate directly the three-dimensional (3D) structure of membranous cell organelles. However, the polarity of the Golgi apparatus (that is, the cis-trans axis) can hardly be determined by SEM alone, because there is no appropriate immunocytochemical method for specific labelling of its cis- or trans-faces. In the present study, we used the osmium impregnation method, which forms deposits of reduced osmium exclusively in the cis-Golgi elements, for preparation of specimens for SEM. The newly developed procedure combining osmium impregnation with subsequent osmium maceration specifically visualised the cis-elements of the Golgi apparatus, with osmium deposits that were clearly detected by backscattered electron-mode SEM. Prolonged osmication by osmium impregnation (2% OsO4 solution at 40 degrees C for 40 h) and osmium maceration (0.1% OsO4 solution at 20 degrees C for 24 h) did not significantly impair the 3D ultrastructure of the membranous cell organelles, including the Golgi apparatus. This novel preparation method enabled us to determine the polarity of the Golgi apparatus with enough information about the surrounding 3D ultrastructure by SEM, and will contribute to our understanding of the global organisation of the entire Golgi apparatus in various differentiated cells.
Lay Description The Golgi apparatus consists of several layers of flattened cisternae with distinct polarity. Its entry and exit sides are termed the cis-faces (or forming) and trans-faces (or maturing), respectively. The 3D fine structure of the Golgi apparatus, as well as the rough endoplasmic reticulum and mitochondria, can be observed by scanning electron microscopy (SEM), after the soluble cytoplasmic proteins have been selectively removed from the cells by immersing the tissue samples in diluted OsO4 for several days (osmium maceration method). We have analysed the 3D organisation of the Golgi apparatus in various differentiated cells by high-resolution SEM of osmium-macerated tissues, and have demonstrated the diversity in the organisation of the Golgi apparatus. However, we have often faced difficulties in specifying the polarity of the Golgi apparatus, because there were no appropriate ways to label the cis- or trans-faces of the Golgi apparatus by SEM. To overcome this problem, in the present study, we used osmium impregnation for preparation of the specimens for SEM. Impregnation of OsO4 into tissue samples at a relatively high temperature (40 degrees C) for several tens of hours resulted in accumulation of osmium deposits exclusively in the cis-Golgi elements. The newly developed procedure combining osmium impregnation with subsequent osmium maceration specifically labelled the cis-elements of the Golgi apparatus with osmium deposits. The intensity of backscattered electron (BSE)-mode signals depends on the atomic number of the specimen composition (osmium, atomic number 76). Osmium accumulated in the cis-Golgi elements could be clearly visualised owing to the intense signals of BSE-mode SEM, compared with the low background signals of the surrounding organic compounds. Prolonged exposure to OsO4 during the impregnation and maceration processes hardly damaged the 3D fine structure of the cell organelles, including the Golgi apparatus. This novel preparation method enabled us to analyse the polarity of the Golgi apparatus by SEM, with enough information about the surrounding 3D ultrastructure. The method will contribute to our understanding of the organisation of the entire Golgi apparatus in various differentiated cells that has rarely been achieved by transmission electron microscopy.

DOI： 10.1111/jmi.12379

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/jmicro/dfv360

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：OXFORD UNIV PRESS  

Although many studies of the Golgi apparatus structure have been performed by light and electron microscopy, the full shape of the Golgi apparatus remained unclear due to the technical limitations of the previously applied microscopy techniques. In this study, we used serial section scanning electron microscopy (SEM) for the morphological study of the Golgi apparatus. This method is useful for three-dimensional (3D) reconstruction of cellular structures without requiring specialized instruments, unlike focused ion beam SEM (FIB-SEM) and serial block face SEM (SBF-SEM). Using the serial section SEM method developed by our laboratory, we investigate the 3D shape of the osmium-impregnated Golgi apparatus in rat epididymal cells, pancreatic acinar cells and gonadotropes. The combination of serial section SEM and a 3D reconstruction technique enabled us to elucidate the entire shape of the Golgi apparatus in these cells. The full shape of the Golgi apparatus in epididymal cells formed a basket-like structure with oval-shaped cisterns, while the Golgi apparatus in an acinar cell from the pancreas was composed of elongated ribbon-like structures that were connected to each other, making a coarse network. The overall image of the Golgi apparatus cisterns from a gonadotrope looked like a spherical cage. This study has clearly shown that entire 3D shape of the Golgi apparatus varies depending on the cell type and that the Golgi cisterns network appears as a single mass located in the large region of the cytoplasm.

DOI： 10.1093/jmicro/dfv360

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/jmi.12379

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SAGE PUBLICATIONS LTD  

Although the osmium maceration method has been used to observe three-dimensional (3D) structures of membranous cell organelles with scanning electron microscopy (SEM), the use of osmium tetroxide for membrane fixation and the removal of cytosolic soluble proteins largely impairs the antigenicity of molecules in the specimens. In the present study, we developed a novel method to combine cryosectioning with the maceration method for correlative immunocytochemical analysis. We first immunocytochemically stained a semi-thin cryosection cut from a pituitary tissue block with a cryo-ultramicrotome, according to the Tokuyasu method, before preparing an osmium-macerated specimen from the remaining tissue block. Correlative microscopy was performed by observing the same area between the immunostained section and the adjacent face of the tissue block. Using this correlative method, we could accurately identify the gonadotropes of pituitary glands in various experimental conditions with SEM. At 4 weeks after castration, dilated cisternae of rough endoplasmic reticulum (RER) were distributed throughout the cytoplasm. On the other hand, an extremely dilated cisterna of the RER occupied the large region of the cytoplasm at 12 weeks after castration. This novel method has the potential to analyze the relationship between the distribution of functional molecules and the 3D ultrastructure in different composite tissues.

DOI： 10.1369/0022155415609099

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：OXFORD UNIV PRESS  

In this study, we introduce scanning electron microscopy (SEM) of semithin resin sections. In this technique, semithin sections were adhered on glass slides, stained with both uranyl acetate and lead citrate, and observed with a backscattered electron detector at a low accelerating voltage. As the specimens are stained in the same manner as conventional transmission electron microscopy (TEM), the contrast of SEM images of semithin sections was similar to TEM images of ultrathin sections. Using this technique, wide areas of semithin sections were also observed by SEM, without the obstruction of grids, which was inevitable for traditional TEM. This study also applied semithin section SEM to correlative light and electron microscopy. Correlative immunofluorescence microscopy and immune-SEM were performed in semithin sections of LR white resin-embedded specimens using a FluoroNanogold-labeled secondary antibody. Because LR white resin is hydrophilic and electron stable, this resin is suitable for immunostaining and SEM observation. Using correlative microscopy, the precise localization of the primary antibody was demonstrated by fluorescence microscopy and SEM. This method has great potential for studies examining the precise localization of molecules, including Golgi- and ER-associated proteins, in correlation with LM and SEM.

DOI： 10.1093/jmicro/dfv042

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BIOMEDICAL RESEARCH PRESS LTD  

Although a number of papers have given useful information on splenic microcirculation by light and/or scanning electron microscopy, controversies remain as to the vascular arrangement, especially in the human spleen. The present study re-examined the microvasculature of the human spleen using a three-dimensional reconstruction of immunohistochemically stained tissue sections, and showed that the central artery does not directly issue follicular arteries in the human spleen; follicular arteries are derived from penicillar arteries outside the follicle and end in the white pulp. We found that the splenic follicle is surrounded by an elaborate system of anastomosed capillaries in both the marginal zone and the superficial layer of the white pulp. Most of these capillaries are also branches of the penicillar arterioles that are issued from the central artery in the same, or a different, white pulp system. Because the endothelia of these capillaries are widely open in the marginal zone, this vascular network may play a major role in supplying blood to the marginal zone. The accumulation of sialoadhesin-positive macrophages was also observed around the vascular network, suggesting an important role for this structure as the front line of immune response.

DOI： 10.2220/biomedres.36.195

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1369/0022155415609099

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER IRELAND LTD  

In contrast to the widely accepted images of the Golgi apparatus as a cup-like shape, the Golgi in pituitary gonadotropes is organized as a spherical shape in which the outer and inner faces are cis- and trans-Golgi elements, respectively. At the center of the spherical Golgi, a pair of centrioles is situated as a microtubule-organizing center from which radiating microtubules isotropically extend toward the cell periphery. This review focuses on the significance of the characteristic organization of the Golgi and microtubule network in gonadotropes, considering the roles of microtubule-dependent membrane transport in the formation and maintenance of the Golgi structure. Because the highly symmetrical organization of the Golgi is possibly perturbed in response to experimental treatments of gonadotropes, monitoring of the Golgi structure in gonadotropes under various experimental conditions will be a novel in vivo approach to elucidate the biogenesis of the Golgi apparatus. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

DOI： 10.1016/j.mce.2013.10.003

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.mce.2013.10.003

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.11410/kenbikyo.49.3_171

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記述言語：英語   出版者・発行元：NATURE PUBLISHING GROUP  

DOI： 10.1038/jid.2013.60

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japan Society of Histological Documentation  

Gonadotropin-releasing hormone (GnRH) agonists exert acutely stimulatory action on gonadotropes, but thereafter suppress paradoxically gonadotropin synthesis and release by receptor desensitization. To examine whether GnRH signaling affects the morphological characteristics in membranous organelles related to the synthesis of gonadotropin, we have analyzed the ultrastructural changes in the ER and Golgi apparatus of male rat pituitary gonadotropes during sustained treatment with a GnRH agonist, leuprorelin. In pituitary gonadotropes at 1 day after the onset of treatment, clusters of the tubuloreticular ER appeared, and the globular Golgi apparatus was transiently disassembled into isolated small-sized stacks. However, 1 week after the onset of the treatment, the tubuloreticular ER was seemingly converted to rough ER with regularly stacked sheets and the scattered Golgi stacks converged to form globular structures. In the following chronic phase of the treatment, the ER cisterns remained flattened and the trans-Golgi compartment appeared to be collapsed. Sustained treatment with leuprorelin could also restore the enlarged Golgi apparatus and expand the cisterns of the rough ER
a feature that was seen in hypertrophic gonadotropes of castrated rats. These findings indicated that the ultrastructure of the membranous organelles changed because of the chronic suppressive effects of leuprorelin on gonadotropes both in the physiological and stimulated states. The acute and chronic ultrastructural changes in the ER and Golgi apparatus during sustained leuprorelin treatment also suggests that GnRH signaling cross-talks with the regulation of the morphological characteristics in membranous organelles related to gonadotropin synthesis.

DOI： 10.1679/aohc.74.41

Scopus

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Axonal injury and demyelination are observed in demyelinating diseases such as multiple sclerosis. However, pathological changes that underlie these morphologies are not fully understood. We examined in vivo morphological changes using a new histological technique, scanning electron microscopy (SEM) with osmium maceration method to observe three-dimensional structures such as myelin and axons in the spinal cord. Myelin basic protein-deficient shiverer mice and mice with experimental autoimmune encephalomyelitis (EAE) were used to visualize how morphological changes in myelin and axons are induced by dysmyelination and demyelination.SEM revealed following morphological changes during dysmyelination of shiverer mice. First, enriched mitochondria and well-developed sER in axons were observed in shiverer, but not in wild-type mice. Second, the processes from some perinodal glial cells ran parallel to internodes of axons in addition to the process that covered the nodal region of the axon in shiverer mice. Last, this technique left myelin and axonal structures undisturbed. Moreover, SEM images showed clear variations in the ultrastructural abnormalities of myelin and axons in the white matter of the EAE spinal cord. This technique will be a powerful tool for identifying the mechanisms underlying the pathogenesis in demyelination. © 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society.

DOI： 10.1016/j.neures.2013.01.009

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PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.neures.2013.01.009

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1679/aohc.74.41

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：INFORMA HEALTHCARE  

Histological changes were observed in peripheral nerves following end-to-side nerve coaptation to determine the effects of perineurial opening and deliberate donor nerve injury during surgery. Twenty rats were randomised into four groups as follows: group 1, end-to-side nerve coaptation without perineurial opening; group 2, end-to-side nerve coaptation with simple perineurial opening; group 3, end-to-side nerve coaptation with partial crush injury after perineurial opening; group 4, end-to-side nerve coaptation with partial neurotomy after perineurial opening. Seven days after coaptation of the musculocutaneous (recipient) nerve to the ulnar (donor) nerve, the nerves were immunohistochemically analysed using antibodies against neurofilament-H (RT97) and phosphorylated GAP-43 (p-GAP-43). The former labels all axons, including regenerating axons and degenerated axonal debris, while the latter only labels regenerating axons. Results demonstrated no regenerating nerves in the recipient nerve of group 1. In group 2, because nerve herniation from the perineurial opening partially injured donor nerve fibres, some regenerating axons extended proximally and distally along the partially injured fibres in the donor nerve; some of these regenerating axons also extended into the recipient nerve via the perineurial opening. In groups 3 and 4, thin regenerating axons were more prominent in recipient and donor nerves compared with group 2. Statistical evaluation revealed increased efficacy of perineurial opening and deliberate donor nerve injury in end-to-side nerve coaptation, suggesting that partial nerve fibre herniation with partial axonotmesis or neurotomesis was important for effective axonal regeneration in end-to-side nerve coaptation.

DOI： 10.3109/2000656X.2012.696264

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PUBLIC LIBRARY SCIENCE  

Reactive oxygen species (ROS) are toxic but essential molecules responsible for host defense and cellular signaling. Conserved NADPH oxidase (NOX) family enzymes direct the regulated production of ROS. Hydrogen peroxide (H2O2) generated by dual oxidases (DUOXs), a member of the NOX family, is crucial for innate mucosal immunity. In addition, H2O2 is required for cellular signaling mediated by protein modifications, such as the thyroid hormone biosynthetic pathway in mammals. In contrast to other NOX isozymes, the regulatory mechanisms of DUOX activity are less understood. Using Caenorhabditis elegans as a model, we demonstrate that the tetraspanin protein is required for induction of the DUOX signaling pathway in conjunction with the dual oxidase maturation factor (DUOXA). In the current study, we show that genetic mutation of DUOX (bli-3), DUOXA (doxa-1), and peroxidase (mlt-7) in C. elegans causes the same defects as a tetraspanin tsp-15 mutant, represented by exoskeletal deficiencies due to the failure of tyrosine cross-linking of collagen. The deficiency in the tsp-15 mutant was restored by co-expression of bli-3 and doxa-1, indicating the involvement of tsp-15 in the generation of ROS. H2O2 generation by BLI-3 was completely dependent on TSP-15 when reconstituted in mammalian cells. We also demonstrated that TSP-15, BLI-3, and DOXA-1 form complexes in vitro and in vivo. Cell-fusion-based analysis suggested that association with TSP-15 at the cell surface is crucial for BLI-3 activation to release H2O2. This study provides the first evidence for an essential role of tetraspanin in ROS generation.

DOI： 10.1371/journal.pgen.1002957

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SAGE PUBLICATIONS LTD  

In polarized exocrine cells, the Golgi apparatus is cup-shaped and its convex and concave surfaces are designated as cis and trans faces, functionally confronting the rough endoplasmic reticulum and the cell surface, respectively. To clarify the morphological characteristics of the Golgi apparatus in non-polarized endocrine cells, the investigators immunocytochemically examined its precise architecture in pituitary gonadotropes, especially in relation to the arrangement of the intracellular microtubule network. The Golgi apparatus in the gonadotropes was not cup-shaped but ball-shaped or spherical, and its outer and inner surfaces were the cis and trans faces, respectively. Centrioles were situated at the center of the Golgi apparatus, from which radiating microtubules isotropically extended to the cell periphery through the gaps in the spherical wall of the Golgi stack. The shape of the Golgi apparatus and the arrangement of microtubules demonstrated in the present study could explain the microtubule-dependent movements of tubulovesicular carriers and granules within the gonadotropes. Furthermore, the spherical shape of the Golgi apparatus possibly reflects the highly symmetrical arrangement of microtubule arrays, as well as the poor polarity in the cell surface of pituitary gonadotropes. (J Histochem Cytochem 60:588-602, 2012)

DOI： 10.1369/0022155412448791

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：OXFORD UNIV PRESS  

Scanning electron microscopy (SEM) using osmium-maceration methods has been used for analyzing the three-dimensional structure of cell organelles in tissue samples, but it has been quite difficult to observe free and cultured cells with this technique. The present study was performed to develop a method that can be applied to free and cultured cells for SEM studies of intracellular structures after osmium maceration. The method was also applied to light microscopy (LM) and to transmission electron microscopy (TEM). HeLa cells and human leukocytes were fixed with a mixture of 0.5% paraformaldehyde and 0.5% glutaraldehyde followed by an additional fixation with 1% osmium tetroxide. These cells were embedded in low-melting-point agarose. A temperature-responsive dish was also used for collection of cultured cells before embedding. For LM and TEM, the cell-embedded agarose was further embedded in epoxy resin, and semi- and ultrathin sections were examined conventionally. For SEM, the agarose was freeze-fractured in 50% dimethyl sulfoxide, processed for osmium maceration and observed in a high-resolution SEM. Low-melting-point agarose was useful as an embedding medium for SEM, because it was well preserved during prolonged osmication for SEM. Thus, the fine structure of cell organelles was clearly analyzed by SEM after osmium-maceration treatment. These SEM images could also be compared with those of LM and TEM of the agarose-embedded tissues.

DOI： 10.1093/jmicro/dfr098

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BLACKWELL PUBLISHING  

Background: We have investigated how recirculating lymphocytes patrol the liver in a normal steady state. Methods: Thoracic duct lymphocytes of congeneic rats were intravenously transferred to host rats and donor cell trafficking in the liver and hepatic lymph was examined. Host hepatic lymph nodes (HLNs) were selectively removed, which allowed liver-derived donor cells to collect in the thoracic duct without transit in the intervening HLNs. Results: The number of donor cells in the thoracic duct lymph significantly increased over the baseline 3, 5 and 11 h after transfer in the HLN-removed, non-pretreated, and HLN-ligated ( in which a lymph efflux was blocked) groups, respectively. Histologically, donor cells appeared in the portal area from 0.5 h after transfer and frequently attached to the basal lamina of portal vein both externally and internally. Three hours after transfer, a few donor cells appeared in the subcapsular sinus of HLNs. Conclusion: The minimal transit time of rat recirculating lymphocytes is 3 - 4 h in the liver and 5 - 8 h in the hepatic LNs, in a normal steady state. Recirculating lymphocytes might transmigrate through the portal vein as well as the sinusoid in the periportal zone. This rapid transit might enable an efficient surveillance of the liver portal area by the recirculating lymphocytes.

DOI： 10.1111/j.1478-3231.2008.01671.x

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：漂着物学会事務局  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：INT SOC HISTOLOGY & CYTOLOGY  

The three-dimensional ultrastructure of the Golgi apparatus in different cells of the rat - epithelial principal cells in the epididymal duct, goblet cells in the jejunum, gonadotrophs in the pituitary grand and dorsal root ganglion cells - was studied by scanning electron microscopy (SEM) of osmium-macerated tissues. The Golgi apparatus in the epididymal principal cells took the shape of a candle flame with irregular-shaped cisterns, while those in the goblet cells of the jejunum were cup-shaped or cylindrical with flat cisterns. Gonadotrophs had a large spherical Golgi apparatus; this apparatus was composed of several concentric cisterns with large round windows through which the rough endoplasmic reticulum (rER) and mitochondria extended into the center of the globular Golgi apparatus. Dorsal root ganglion cells had several small Golgi stacks scattered in the cytoplasm. In all Golgi apparatuses of the different cells examined in the present study, the cis-most cistern was generally composed of a flattened sheet with numerous small fenestrations on its wall. On the other hand, the shape of the trans-most cistern varied by cell type; it was generally composed of tubules and/or small sheets which were sometimes connected with each other to form a rather complicated structure. The cismost cistern and the trans-most cistern were often closely associated with the rER although no direct communication was found between them. These findings indicate that the structure of the Golgi apparatus, especially its overall shape and the ultrastructure of the trans-most cistern, varies by cell type, a point to be considered in relation to the function of the individual cells.

DOI： 10.1679/aohc.69.357

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：OXFORD UNIV PRESS  

The ptilinum of the fly and the compound eye are among the most fragile organs encountered during conventional procedures of morphological sample fixation. in order to identify a fixative suitable for preparing such samples for scanning electron microscopy, we examined various fixation conditions using microwave irradiation (MWI). The conditions examined were: (i) fixatives; (ii) temperature; (iii) concentration; (iv) duration; (v) dehydration; and (vi) substitution. The identified optimal conditions were 5% glutaraldehydc with MWI (350 W, 5 min). The MWI was continued until the maximal temperature of 75degreesC was attained, followed by intermittent irradiation to maintain a temperature of 75degreesC. After irradiation, the sample was left at room temperature for 24 h in the fixative and then dehydrated in increasing concentrations of ethanol. Each step in the ethanol series lasted for 24 h. The final absolute ethanol step included three solution changes, with each incubation lasting 1 h. A subsequent stepwise substitution of t-butyl alcohol for ethanol was conducted by reducing the ratio of 100% ethanol to t-butyl alcohol from 2:1 to 1: 1 and then 1:2 (24 h each). The substitution was completed by three solution changes using 100% t-butyl alcohol, 30 min each. The best results were obtained by freeze-drying samples using t-butyl alcohol. The use of MWI improved fixative permeation, which occurred at a uniform rate throughout the sample. Comparison with temperature in a water bath at 75degreesC indicated that the fixation effect of MWI was due to its heat generation in addition to some unknown mechanism.

DOI： 10.1093/jmicro/52.5.477

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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担当区分：筆頭著者   記述言語：日本語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：公益社団法人　日本顕微鏡学会  

記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語   出版者・発行元：(一社)日本解剖学会  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語  

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記述言語：日本語  

J-GLOBAL

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記述言語：英語   出版者・発行元：日本神経化学会  

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記述言語：日本語   出版者・発行元：(一社)日本解剖学会  

J-GLOBAL

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語   出版者・発行元：(一社)日本解剖学会  

J-GLOBAL

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記述言語：日本語   出版者・発行元：(一社)日本解剖学会  

J-GLOBAL

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記述言語：日本語   出版者・発行元：(一社)日本解剖学会  

J-GLOBAL

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記述言語：日本語   出版者・発行元：ニュートンプレス  

CiNii Books

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開催年月日： 2022年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催年月日： 2021年12月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（公募）  

開催年月日： 2023年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：東北大学  

開催年月日： 2019年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催年月日： 2019年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：順天堂大学本郷キャンパス  

開催年月日： 2019年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：品川インターシティ  

開催年月日： 2018年11月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：パシフィコ横浜  

開催年月日： 2018年8月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催年月日： 2018年8月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：国民宿舎「桂浜荘」  

開催年月日： 2018年5月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：久留米シティプラザ  

開催年月日： 2018年5月

記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：久留米シティプラザ  

開催年月日： 2018年5月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催年月日： 2016年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：ビックパレふくしま  

開催年月日： 2015年12月

記述言語：日本語   会議種別：口頭発表（招待・特別）  

開催年月日： 2015年9月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催年月日： 2015年8月

記述言語：日本語   会議種別：口頭発表（一般）  

開催年月日： 2015年5月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

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記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

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記述言語：英語  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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受賞区分：国内外の国際的学術賞  受賞国：日本国

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受賞区分：国内学会・会議・シンポジウム等の賞  受賞国：日本国

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受賞区分：国内学会・会議・シンポジウム等の賞 

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受賞区分：国内学会・会議・シンポジウム等の賞 

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受賞区分：国際学会・会議・シンポジウム等の賞 

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受賞区分：国際学会・会議・シンポジウム等の賞 

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受賞区分：国内学会・会議・シンポジウム等の賞 

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受賞区分：国内学会・会議・シンポジウム等の賞 

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NHK科学番組に電子顕微鏡の画像を提供した。

電子顕微鏡の実機をサイパルに持ち運び、旭川市民へ電子顕微鏡の世界を紹介した。アウトリーチ活動

種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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NHKスペシャル「人体」に提供した画像についてや、「ミクロの世界の魅力」について、トークショーというスタイルで子供達や一般の方々に紹介した。

種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：講演会

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：講演会

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魔法の虫メガネ　走査電子顕微鏡の魅力～NHKスペシャル人体収録エピソード～、と題して、小学生から大人まで幅広い年代層に、電子顕微鏡の魅力について講演した。

種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：その他

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：その他

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種別：インターネット

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種別：その他

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種別：セミナー・ワークショップ

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：テレビ・ラジオ番組

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種別：講演会

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対象： 小学生,　中学生

種別：講演会

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種別：出前授業

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種別：出前授業

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種別：出前授業

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種別：出前授業

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対象： 高校生

種別：出前授業

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種別：出前授業

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種別：出前授業

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