2025/04/05 更新

写真a

ウシオダ リョウヘイ
潮田 亮平
USHIODA Ryohei
所属
病院 診療科 外科(心臓大血管)
外部リンク

学歴

  • 旭川医科大学   医学部

    - 2017年4月

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    国名: 日本国

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論文

  • Hypothermic circulatory arrest at 20 ℃ does not deteriorate coagulopathy compared to 28 ℃ in a pig model.

    Hayato Ise, Kyohei Oyama, Ryohei Ushioda, Aina Hirofuji, Keisuke Kamada, Yuri Yoshida, Payam Akhyari, Hiroyuki Kamiya

    Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs   28 ( 1 )   36 - 42   2025年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    It is believed that a lower temperature setting of hypothermic circulatory arrest (HCA) in thoracic aortic surgery causes coagulopathy, resulting in excessive bleeding. However, experimental studies that eliminate clinical factors are lacking. The objective of this study is to investigate the influence of the temperature setting of HCA on coagulation in a pig model. Ten pigs were divided into the following two groups: moderate temperature at 28 °C (group M, n = 5) or lower temperature at 20 °C (group L, n = 5). Two hours of HCA during a total of 4 h of cardiopulmonary bypass (CPB) were performed. Blood samples were obtained at the beginning (T1) and the end (T2) of the surgery, and coagulation capability was analyzed through standard laboratory tests (SLTs) and rotational thromboelastometry (ROTEM). In SLTs, hemoglobin, fibrinogen, platelet count, prothrombin time, and activated partial thromboplastin time were analyzed. In ROTEM analyses, clotting time and clot formation time of EXTEM, maximum clot firmness (MCF), and maximum clot elasticity (MCE) of EXTEM and FIBTEM were analyzed. Fibrinogen decreased significantly in both groups (group M, p = 0.008; group L, p = 0.0175) at T2, and FIBTEM MCF and MCE also decreased at T2. There were no differences regarding changes in parameters of SLTs and ROTEM between groups. CPB decreases coagulation capacity, contributed by fibrinogen. However, a lower temperature setting of HCA at 20 °C for 2 h did not significantly affect coagulopathy compared to that of HCA at 28 °C after re-warming to 37 °C.

    DOI: 10.1007/s10047-024-01449-9

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  • Transapical aortic valve replacement and concomitant coronary bypass grafting in on-pump beating fashion: a case report. 国際誌

    Shougo Takahashi, Kentaro Shirakura, Masahiro Tsutsui, Shingo Kunioka, Ryohei Ushioda, Yuya Kitani, Akiho Minoshima, Toshiharu Takeuchi, Naoki Nakagawa, Hiroyuki Kamiya

    Journal of surgical case reports   2025 ( 3 )   rjaf119   2025年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Herein, we report a case of transapical transcatheter aortic valve replacement and coronary artery bypass grafting performed in an on-pump beating fashion in an old woman with severe aortic valve stenosis, a porcelain aorta, severe calcified coronary artery disease, and a history of abdominal aortic replacement.

    DOI: 10.1093/jscr/rjaf119

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  • Relationships among hemolysis indicators and neuron-specific-enolase in patients undergoing veno-arterial extracorporeal membrane oxygenation.

    Ryo Okubo, Tomonori Shirasaka, Ryohei Ushioda, Masahiko Narita, Shingo Kunioka, Yuta Kikuchi, Masahiro Tsutsui, Nobuya Motoyoshi, Hiroyuki Kamiya

    Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs   28 ( 1 )   43 - 49   2025年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Neuron-specific-enolase is used as a marker of neurological prognosis after cardiopulmonary resuscitation. It is also present in red blood cells and platelets. It is not known whether hemolysis increases the values of neuron-specific-enolase enough to clinically affect its interpretation in critically ill patients who are to be introduced to veno-arterial extracorporeal oxygenation. In this study, we examined the relationships among neuron-specific-enolase and hemolysis indicators such as free hemoglobin and lactate dehydrogenase after the introduction of veno-arterial extracorporeal oxygenation. Of the 91 patients who underwent veno-arterial extracorporeal membrane oxygenation in our hospital from January 1, 2018, to February 24, 2021, 68 patients survived for more than 24 h. Of these, 14 patients who were categorized into the better cerebral performance categories (1-3) and 19 patients who were categorized into the poor neurological prognosis category (4) were included. After the introduction of veno-arterial extracorporeal membrane oxygenation, neuron-specific-enolase was markedly higher in the poor neurological prognosis group than in the good neurological prognosis group (41.6 vs. 92.0, p = 0.04). A significant positive correlation was revealed between neuron-specific-enolase and free hemoglobin in the good neurological prognosis group (rs = 0.643, p = 0.0131). A similar relationship was observed for lactate dehydrogenase and neuron-specific-enolase in both the conscious (rs = 0.737, p = 0.00263) and non-conscious groups (rs = 0.544, p = 0.0176). When neuron-specific-enolase is used as a marker for neuroprognostic evaluation, an abnormally high value is likely to indicate the lack of consciousness, whereas a lower elevation should be interpreted with caution, taking into account the effects of hemolysis.

    DOI: 10.1007/s10047-024-01454-y

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  • A case of rapid rupture of a calcified amorphous tumor observed by echocardiography. 国際誌

    Kentaro Shirakura, Ryohei Ushioda, Masahiro Tsutsui, Shingo Kunioka, Nobuhiro Mochizuki, Tatsuya Aonuma, Naoko Kawabata, Erika Saitoh, Naoki Nakagawa, Hiroyuki Kamiya

    Journal of surgical case reports   2025 ( 2 )   rjaf064   2025年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A calcified amorphous tumor (CAT) of the heart is a rare, non-neoplastic, intracavitary cardiac mass. Histological examination reveals the presence of calcified and amorphous fibrous material with underlying chronic inflammation. Some studies have reported that CAT typically exhibits rapid growth. However, we observed a case in which CAT unexpectedly ruptured within approximately two weeks. There was no cerebral infarction or significant valvular disease, therefore we were not sure about the indication for surgery; however considering the epidemiological possibility of CAT, we decided to operate and were able to treat the patient without complications.

    DOI: 10.1093/jscr/rjaf064

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  • Transesophageal Echocardiographic Imaging of Right Coronary Blood Flow With and Without Left Ventricular Decompression. 国際誌

    Yasuhiro Suda, Miho Takemitsu, Ryohei Ushioda, Hiroshi Makino

    Journal of cardiothoracic and vascular anesthesia   38 ( 11 )   2847 - 2849   2024年11月

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  • Axillary-coronary artery bypass using a vein graft via the suprasternal route. 国際誌

    Hideki Isa, Masahiro Tsutsui, Fumitaka Suzuki, Ryohei Ushioda, Shingo Kunioka, Hiroyuki Kamiya

    Asian cardiovascular & thoracic annals   32 ( 8-9 )   481 - 483   2024年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Redo coronary artery bypass grafting after an in-situ right internal thoracic artery graft to the left anterior descending artery is challenging. For such a 52-year-old male patient with a history of mediastinitis, we performed redo bypass grafting of the right coronary artery using a saphenous vein graft and xiphoid resection via the suprasternal route with the left axillary artery as the inflow source and the graft were patent. The axillary artery is an inflow source for patients with inaccessible aorta. Combining the suprasternal route with a xiphoid resection provides a bail-out option.

    DOI: 10.1177/02184923241301109

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  • Giant cardiac schwannoma around the left atrium: a case report. 国際誌

    Ryohei Ushioda, Boonsap Sakboon, Dit Yoongtong, Jaroen Cheewinmethasiri, Hiroyuki Kamiya, Nuttapon Arayawudhikul

    Journal of surgical case reports   2024 ( 11 )   rjae738   2024年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A 57-year-old male presented with dyspnea and an enlarged cardiac silhouette on a chest X-ray. Further evaluation with contrast-enhanced computed tomography revealed a giant heterogeneous mediastinal mass, ~8.9 × 7.3 × 12.2 cm, with peripheral calcifications. Surgical resection was performed via a left thoracotomy approach using the left fifth intercostal space. Cardiopulmonary bypass was established through the femoral vessels for safer and more controlled resection. The tumor, contiguous with the left atrium, was successfully excised using two Endo GIA staplers. Pathological examination confirmed the diagnosis of schwannoma. This case demonstrates that the left thoracotomy approach with cardiopulmonary bypass and the use of Endo GIA staplers is a feasible and effective option for resecting large, well-defined cardiac schwannomas.

    DOI: 10.1093/jscr/rjae738

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  • A case of minimum invasive debranch thoracic endovascular aortic repair for isolated left vertebral artery: complete revascularization without artificial vessels via a single small incision. 国際誌

    Masahiro Tsutsui, Kazuki Miyatani, Kentaro Shirakura, Yuki Setogawa, Fumitaka Suzuki, Hiroyuki Miyamoto, Ryo Okubo, Ryohei Ushioda, Shingo Kunioka, Natsuya Ishikawa, Norihumi Otani, Hiroyuki Kamiya

    Journal of surgical case reports   2024 ( 10 )   rjae595   2024年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Isolated left vertebral artery (ILVA) is one of the most frequent vertebral abnormalities. When performing thoracic endovascular aortic repair (TEVAR), the ILVA may have to be closed depending on the position of the stent graft; in these cases, the decision to reconstruct the ILVA depends on the state of cerebral blood flow. Here, we report a case of a 68-year-old male, in whom the Willis arterial circle was incomplete; we therefore performed a reconstructive method during zone 2-landing TEVAR that ensured ILVA and left subclavian artery blood flow without the use of artificial vessels. Only one supraclavicular incision was required for reconstruction. This method has some procedural difficulties; however, it does not use artificial blood vessels and can be performed with a single incision.

    DOI: 10.1093/jscr/rjae595

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  • Successful surgical repair of a huge left ventricular pseudoaneurysm after repair of left ventricular rupture during mitral valve replacement. 国際誌

    Kyoka Hayashi, Ryohei Ushioda, Jun Maruoka, Kaname Shimizu, Kentaro Shirakura, Nobuhiro Mochizuki, Yuki Setogawa, Ryo Okubo, Miyamoto Hiroyuki, Shougo Takahashi, Shingo Kunioka, Masahiro Tsutsui, Kamiya Hiroyuki

    Journal of surgical case reports   2024 ( 10 )   rjae636   2024年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A 78-year-old man underwent pericardial patch repair for left ventricular (LV) rupture during mitral valve replacement. After the first operation, a huge (>10 cm) LV pseudoaneurysm was detected, necessitating reoperation. LV rupture is a rare but often fatal complication of mitral valve replacement. Although repair of LV rupture during mitral valve replacement has been reported, the development of pseudoaneurysm after such repair is exceedingly rare. In this case, we successfully treated a huge LV pseudoaneurysm using two pericardial patches to sandwich the rupture hole from the inside.

    DOI: 10.1093/jscr/rjae636

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  • Epicardial hematoma due to heart positioner device in minimally invasive coronary artery bypass. 国際誌

    Nobuhiro Mochizuki, Ryohei Ushioda, Dit Yoongtong, Boonsap Sakboon, Jaroen Cheewinmethasiri, Hiroyuki Kamiya, Nuttapon Arayawudhikul

    Journal of surgical case reports   2024 ( 6 )   rjae417   2024年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Minimally invasive cardiac surgery off-pump coronary artery bypass (MICSOPCAB) has become increasingly prevalent, with devices like the heart positioner aiding in surgical precision. However, rare complications such as epicardial hematoma can occur. Here, we present a case of a 75-year-old man undergoing MICSOPCAB who developed an epicardial hematoma due to the heart positioner. The hematoma was successfully repaired intraoperatively with direct suturing and large felts. Postoperative recovery was uneventful, highlighting the importance of vigilant monitoring and prompt management of such complications. This case underscores the need for careful attention during the use of cardiac positioners to minimize adverse events and ensure favorable patient outcomes.

    DOI: 10.1093/jscr/rjae417

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  • Fulminant respiratory failure due to severe pneumothorax after re-do coronary artery bypass grafting treated with veno-venous extracorporeal membrane oxygenation. 国際誌

    Akito Inoue, Ryohei Ushioda, Kazuki Miyatani, Kentaro Shirakura, Nobuhiro Mochizuki, Hideki Isa, Yuki Setogawa, Masahiko Narita, Fumitaka Suzuki, Aina Hirofuji, Ryo Okubo, Shingo Kunioka, Masahiro Tsutsui, Kamiya Hiroyuki

    Journal of surgical case reports   2024 ( 5 )   rjae360   2024年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This case report details the management of a 79-year-old man who developed massive postoperative pneumothorax following redo coronary artery bypass grafting due to severe lung adhesions. We successfully treated the patient using veno-venous extracorporeal membrane oxygenation without femoral cannulation, allowing for early rehabilitation initiation. Veno-venous extracorporeal membrane oxygenation is a reasonable option for cases of severe respiratory failure due to pneumothorax with lung destruction caused by re-sternotomy during re-do cardiac surgery.

    DOI: 10.1093/jscr/rjae360

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  • A case of non-bacterial thrombotic endocarditis on the aortic valve following coronary angiography. 国際誌

    Nobuhiro Motiduki, Ryohei Ushioda, Sayaka Yuzawa, Kazuki Miyatani, Hideki Isa, Yuki Setogawa, Kohei Ishidou, Masahiko Narita, Fumitaka Suzuki, Aina Hirofuji, Ryo Okubo, Shingo Kunioka, Masahiro Tsutsui, Natsuya Ishikawa, Kamiya Hiroyuki

    Journal of surgical case reports   2024 ( 4 )   rjae212   2024年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nonbacterial thrombotic endocarditis (NBTE) on the aortic valve involves fibrin and platelet aggregate formation, potentially leading to embolic events. We present a case of NBTE on the aortic valve following coronary angiography (CAG) in a 54-year-old man with multiple comorbidities. Surgical thrombectomy was performed owing to acute cerebral infarcts. This case highlights the significance of considering that mechanical trauma from catheterization during CAG can trigger thrombus formation.

    DOI: 10.1093/jscr/rjae212

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  • The left axillary artery is a reasonable option as the inflow site for saphenous vein graft in minimally invasive coronary artery bypass grafting. 国際誌

    Ryohei Ushioda, Aina Hirofuji, Dit Yoongtong, Boonsap Sakboon, Jaroen Cheewinmethasiri, Thanin Lokeskrawee, Jayanton Patumanond, Suppachai Lawanaskol, Hiroyuki Kamiya, Nuttapon Arayawudhikul

    Frontiers in cardiovascular medicine   11   1397396 - 1397396   2024年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    INTRODUCTION: This study aims to clarify the good inflow site for saphenous vein grafts (SVG) in minimally invasive off-pump coronary artery bypass grafting (mini-CABG), between the ascending aorta, the internal thoracic arteries (ITAs) and the left axillary artery (LAA). METHODS: This retrospective study included 126 patients who underwent Mini-CABG at our center between January 2014 and July 2023. Patients were divided into three groups according to the SVG inflow site for patency comparison: Aorta group (n = 56), LAA group (n = 23), and ITA group (n = 47). RESULTS: There were 84 males, with mean age of 65.9 ± 7.0 years. There were no significant differences in preoperative characteristics between groups. Mean operation times were 254.6 ± 72.2, 213.7 ± 57.6, and 253.0 ± 81.2 min, and the average numbers of distal anastomoses were 2.9 ± 0.9, 2.4 ± 0.7 and 2.9 ± 1.1 in the Aorta, ITA and LAA groups respectively. Days in intensive care, hospital stay, and major complications did not differ between the groups. Early patency of SVG did not significantly differ among groups: 93.0% in the Aorta group, 98.0% in the ITA group, and 100% in the LAA group. Mean follow-up period was 136.7 ± 295.7 days, and follow-up coronary CTA revealed 18 SVG occlusions (Aorta group n = 8, ITA group n = 5, LAA group n = 5). The Kaplan-Meier curve for SVG patency rates did not show any significant differences among the three groups. CONCLUSION: The ascending aorta, the ITAs, and the LAA serve as reliable inflow sites with similar results in mini-CABG.

    DOI: 10.3389/fcvm.2024.1397396

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  • Assessing the benefits of anaortic off-pump coronary artery bypass grafting. 国際誌

    Ryohei Ushioda, Aina Hirofuji, Dit Yoongtong, Boonsap Sakboon, Jaroen Cheewinmethasiri, Thanin Lokeskrawee, Jayanton Patumanond, Suppachai Lawanaskol, Hiroyuki Kamiya, Nuttapon Arayawudhikul

    Frontiers in cardiovascular medicine   11   1393921 - 1393921   2024年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    INTRODUCTION: The procedure called the "aorta no-touch" (NT) or anaortic technique in off-pump coronary artery bypass grafting (OPCAB) is designed to reduce the perioperative risk of stroke. We have observed an increased frequency of anaortic OPCAB procedures at our institution. The main purpose of the present study is to investigate the effectiveness of anaortic OPCAB in reducing the perioperative risk of stroke. METHODS: From April 2011 to July 2023, a total of 2,236 patients underwent isolated OPCAB at our single center. The patients were divided into the anaortic group (NT, n = 762) and the aortic group (A, n = 1,474). The NT group was propensity score-matched (PSM) with the A group at a 1:1 ratio (NT n = 640; A n = 640), and matching was performed based on 26 covariates with preoperative clinical characteristics. RESULTS: In both the unmatched and matched cohorts of the NT and A groups, there were no significant differences observed in new stroke rates (NT vs. A; unmatched, 1.0% vs. 1.2%, p = 0.624; matched, 0.9% vs. 1.3%, p = 0.789). The univariable logistic analysis did not identify the anaortic technique as an independent factor negatively associated with new stroke events (OR = 0.81, 95% CI = 0.35-1.86, p = 0.624). CONCLUSION: The present study did not find the anaortic technique to reduce the perioperative risk of stroke in OPCAB. Hence, further large studies are needed to identify patient cohorts in which anaortic OPCAB is significantly beneficial.

    DOI: 10.3389/fcvm.2024.1393921

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  • Cardiopulmonary resuscitation at operating room entry in acute aortic dissection type A patients: is surgery contraindicated? 国際誌

    Hideki Isa, Taro Kanamori, Kazuki Miyatani, Masahiro Tsutsui, Ryohei Ushioda, Shota Yamanaka, Hiroyuki Kamiya

    Frontiers in surgery   11   1404825 - 1404825   2024年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: This study aimed to compare the short-term outcomes of surgical treatment for acute type A aortic dissection between patients undergoing cardiopulmonary arrest at the time of entry into the operating room and patients who received successful preoperative cardiopulmonary resuscitation before entering the operating room or patients who had cardiopulmonary arrest on the operating room table after entering the operating room without cardiopulmonary arrest. In the present study, we focused on the circulatory status at the time of entering the operating room because it is economically and emotionally difficult to cease intervention once the patient has entered the operating room, where surgeons, anesthesiologists, nurses, and perfusionists are already present, all necessary materials are packed off and cardiopulmonary bypass have already been primed. METHODS: Twenty (5.5%) of 362 patients who underwent surgical treatment for acute type A aortic dissection between January 2016 and March 2022 had preoperative cardiopulmonary arrest. To compare the early operative outcomes, the patients were divided into the spontaneous circulation group (n = 14, 70.0%) and the non-spontaneous circulation group (n = 6, 30.0%) based on the presence or absence of spontaneous circulation upon entering the operating room. The primary endpoint was postoperative 30-day mortality. The secondary endpoints included in-hospital complications and persistent neurological disorders. RESULTS: Thirty-day mortality was 65% (n = 13/20) in the entire cohort; 50% (n = 7/14) in the spontaneous circulation group and 100% (n = 6/6) in the non-spontaneous circulation group. The major cardiopulmonary arrest causes were aortic rupture and cardiac tamponade (n = 16; 80.0%), followed by coronary malperfusion (n = 4; 20.0%). Seven patients (50.0%) survived in the spontaneous circulation group, and none survived in the non-spontaneous circulation group (P = .044). Five survivors walked unaided and were discharged home; the remaining two were comatose and paraplegic. CONCLUSIONS: The outcomes were extremely poor in patients with acute type A aortic dissection who had preoperative cardiopulmonary arrest and received ongoing cardiopulmonary resuscitation at entry into the operating room. Therefore, surgical treatment might be contraindicated in such patients.

    DOI: 10.3389/fsurg.2024.1404825

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  • Off-pump minimally invasive coronary artery bypass grafting in patients with left ventricular dysfunction: the lampang experience. 国際誌

    Ryohei Ushioda, Aina Hirofuji, Dit Yoongtong, Boonsap Sakboon, Jaroen Cheewinmethasiri, Hiroyuki Kamiya, Nuttapon Arayawudhikul

    Frontiers in surgery   11   1324343 - 1324343   2024年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    INTRODUCTION: The minimally invasive cardiac surgery off-pump coronary artery bypass (MICSOPCAB) is technically difficult; therefore, previous studies have indicated that MICSOPCAB should be contraindicated in patients with impaired left ventricular (LV) function. In this study, we investigated the feasibility of MICSOPCAB in patients with impaired LV function. METHODS: The 226 patients underwent MICSOPCAB between August 2017 and September 2022. Our study defined impaired LV function as ejection fraction (EF) in echocardiography 40% or less. The patients were divided into Low EF group (n = 39) and Normal EF group (n = 187). RESULTS: The Low EF group was in a more critical preoperative condition than Normal EF group (41.0% in the Low EF group vs. 14.4% in the Normal EF group; p < 0.001). For preoperative transthoracic echocardiography, LV end-diastolic diameter (5.5 ± 0.9 cm in the Low EF group vs. 5.0 ± 0.8 cm in the Normal EF group; p < 0.001) and LV end-systolic diameter (4.4 ± 1.0 cm in the Low EF group vs. 3.4 ± 1.0 cm in the Normal EF group; p < 0.001) were significantly larger in the Low EF group. No differences were found in the operative time (180 [160-240] min in the Low EF group vs. 205 [165-253] min in the Normal EF group; p = 0.231) and the median number of distal anastomoses (2 [1-2] in the Low EF group vs. 2 [1-3] in the Normal EF group; p = 0.073). Intensive care unit stay was longer in the Low EF group than in the Normal EF group (2 [1-2] in the Low EF group vs. 1 [1-2] in the Normal EF group; p = 0.010). Perioperative transfusion was more common in the Low EF group than in the Normal EF group (69.7% vs. 49.2%; p = 0.023). There were no differences in major complications, hospital stay, and 30-day mortality. The Kaplan-Meier curve showed no significant difference in postoperative major adverse cardiac or cerebrovascular events rates between the two groups (p = 0.185). CONCLUSION: In this study, MICSOPCAB can be performed in patients with low EF having short- and mid-term outcomes similar to patients with normal EF. Therefore, low EF should not be contraindicated in MICSOPCAB.

    DOI: 10.3389/fsurg.2024.1324343

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  • Multi-vessel coronary artery grafting: analyzing the minimally invasive approach and its safety. 国際誌

    Ryohei Ushioda, Aina Hirofuji, Dit Yoongtong, Boonsap Sakboon, Jaroen Cheewinmethasiri, Thanin Lokeskrawee, Jayanton Patumanond, Suppachai Lawanaskol, Hiroyuki Kamiya, Nuttapon Arayawudhikul

    Frontiers in cardiovascular medicine   11   1391881 - 1391881   2024年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    INTRODUCTION: At our institution, we perform off-pump coronary artery bypass (OPCAB) as a standard procedure. Moreover, patients with favorable coronary anatomy and condition are selected for minimally invasive cardiac surgery (MICS)-OPCAB. We retrospectively compared early outcomes, focusing on safety, between MICS-OPCAB and conventional off-pump techniques for multivessel coronary artery bypass grafting (CABG). METHODS: From August 2017 to September 2022, 1,220 patients underwent multivessel coronary artery grafting at our institution. They were divided into the MICS-OPCAB group (MICS group = 163 patients) and the conventional OPCAB group (MS group = 1057 patients). Propensity score matching (1 : 1 ratio) was applied to the MICS-OPCAB and MS groups (149 patients per group) based on 23 preoperative clinical characteristics. RESULTS: After matching, there were no significant differences in preoperative characteristics between the groups. The MICS group had a lower total graft number (2.3 ± 0.6 vs. 2.9 ± 0.8, p < 0.001) and fewer distal anastomoses (2.7 ± 0.8 vs. 3.2 ± 0.9, p < 0.001). There were no significant differences in hospital stay, intensive care unit stay, postoperative complications, and 30-day mortality. The MICS group had less drain output (MICS 350 ml [250-500], MS 450 ml [300-550]; p = 0.013). Kaplan-Meier analysis revealed no significant differences in postoperative MACCE (major adverse cardiac or cerebrovascular events)-free and survival rates between the groups (MACCE-free rate p = 0.945, survival rate p = 0.374). CONCLUSION: With proper patient selection, MICS-OPCAB can provide good short to mid-term results, similar to those of conventional OPCAB.

    DOI: 10.3389/fcvm.2024.1391881

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  • Two-stage repair for DeBakey type II acute aortic dissection and distal aortic arch aneurysm in a nonagenarian patient. 国際誌

    Kentaro Shirakura, Shingo Kunioka, Kazuki Miyatani, Nobuhiro Mochizuki, Hideki Isa, Yuki Setogawa, Fumitaka Suzuki, Ryo Okubo, Ryohei Ushioda, Aina Hirofuji, Masahiro Tsutsui, Natsuya Ishikawa, Hiroyuki Kamiya

    Journal of surgical case reports   2023 ( 12 )   rjad648   2023年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Although total arch replacement would be performed in a patient with acute type A aortic dissection and concomitant aortic aneurysm in the distal aortic arch, total arch replacement may be too invasive in elderly patients with significant morbidities. A 92-year-old female with acute type II DeBakey aortic dissection and concomitant distal aortic arch aneurysm was successfully treated with hemi-arch replacement followed by thoracic endovascular aortic repair. Hybrid two-stage repair of DeBakey type II aortic dissection complicated by distal arch aneurysm using thoracic endovascular aortic repair after hemi-arch replacement may be effective.

    DOI: 10.1093/jscr/rjad648

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  • Bail-Out TEVAR through a Branch of Four-Arm Dacron Graft for Misdeployment of a Frozen Elephant Trunk Prosthesis. 国際誌

    Yuta Kikuchi, Masahiro Tsutsui, Ryohei Ushioda, Tomonori Shirasaka, Hiroyuki Kamiya

    The International journal of angiology : official publication of the International College of Angiology, Inc   32 ( 4 )   308 - 311   2023年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The frozen elephant trunk (FET) is an effective method for making the distal anastomosis more proximal and facilitating aortic remodeling in acute aortic dissection. However, misdeployment of FET to the false lumen has been reported in several cases. Such cases are usually treated with bail-out thoracic endovascular aortic repair (TEVAR) through the femoral artery or additional FET under direct vision to redirect the blood flow to the true lumen. We encountered a case of misdeployment of FET into the false lumen during open aortic surgery for the treatment of Stanford type A acute aortic dissection. After reconstruction of the aorta and all arch vessels, we performed antegrade bail-out TEVAR through a side branch of the four-arm Dacron graft as main access using a pull-through technique through the right femoral artery, which was perfused from the true lumen. This technique, which uses a Dacron graft branch for stent graft access, enabled us to confirm the true lumen because the distal anastomotic site was definitely the true lumen, and we were also able to avoid access difficulties at the iliac artery.

    DOI: 10.1055/s-0042-1747672

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  • Impella Implantation as a Bridge to Surgery for Repair of Aorto-Right Ventricular Fistula Following Prosthetic Valve Endocarditis: A Case Report. 国際誌

    Masahiko Narita, Masahiro Tsutsui, Ryohei Ushioda, Yuta Kikuchi, Tomonori Shirasaka, Hiroyuki Kamiya

    The heart surgery forum   26 ( 4 )   E311-E315   2023年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    For patients with cardiogenic shock, delaying surgery with mechanical circulatory support is reported to yield better outcomes than emergency surgery. We report on an 82-year-old man diagnosed with vertebral osteomyelitis with concomitant infective endocarditis. Chest radiographs revealed a growing abscess, which resulted in an aorto-right ventricular fistula. Providing Impella support allowed for hemodynamic stabilization prior to surgery. The patient had an uneventful postoperative course and reported to be well in a follow-up 1 year later. Impella support can be used as a bridge to surgery for repairing fistulous tract formation in patients in cardiogenic shock.

    DOI: 10.59958/hsf.5519

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  • Evaluating Short-Term Postoperative Outcomes in Minimally Invasive Mitral Valve Surgery for Patients with Rheumatic Disease. 国際誌

    Ryohei Ushioda, Tomonori Shirasaka, Boonsap Sakboon, Jaroen Cheewinmethasiri, Dit Yoongtong, Aina Hirofuji, Hiroyuki Kamiya, Nuttapon Arayawudhikul

    The heart surgery forum   26 ( 2 )   E178-E182   2023年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Minimally invasive mitral valve surgery (MIMVS) is widespread and has become a standard procedure in cardiac surgery [Chitwood 1997; Carpentier 1996]. Therefore, MIMVS is a common procedure for patients with degenerative disease [Raanani 2010; Iribarne 2010] as well. However, the safety of MIMVS in patients with rheumatic heart disease (RHD) has not thoroughly been investigated, due to the low prevalence of RHD in developed countries, where MIMVS is standardized [Miceli 2015]. Here, we investigated the safety of MIMVS for patients with RHD at Lampang Hospital in Thailand.

    DOI: 10.1532/hsf.5391

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  • Coronary reoperation with a free internal mammary artery connected to the right coronary artery as an inflow site; a coronary-to-coronary bypass. 国際誌

    Ryohei Ushioda, Tomonori Shirasaka, Dit Yoongtong, Boonsap Sakboon, Jaroen Cheewinmethasiri, Hiroyuki Kamiya, Nuttapon Arayawudhikul

    Journal of surgical case reports   2023 ( 4 )   rjad136   2023年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Second-time coronary artery bypass grafting is sometimes technically challenging due to severe adhesion of the heart, difficulty of identifying target coronary arteries, advanced sclerosis of the ascending aorta and limited availability of graft vessels. Here we report a patient, in whom a coronary-to-coronary bypass grafting from the native right coronary artery to the left anterior descending artery using a free right internal mammary artery was used as a graft conduit.

    DOI: 10.1093/jscr/rjad136

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  • Internal thoracic artery graft ipsilateral to an arteriovenous fistula for hemodialysis as a sole graft inflow in coronary bypass surgery: A case report. 国際誌

    Masahiko Narita, Tomonori Shirasaka, Ryohei Ushioda, Hiroyuki Kamiya

    JTCVS techniques   16   94 - 95   2022年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.xjtc.2022.09.010

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  • Triplex vascular prostheses elongation in post-operative course. 国際誌

    Masahiko Narita, Tomonori Shirasaka, Ryohei Ushioda, Hiroyuki Kamiya

    Journal of surgical case reports   2022 ( 6 )   rjac255   2022年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Triplex (Terumo Corp, Tokyo, Japan) is a relatively new vascular protheses with a non-biodegradable coating material. We experienced two cases of graft elongation in Triplex grafts post-operatively. In one of the cases, the graft elongation led to occlusion of the left subclavian artery. In the other case, the graft elongation resulted in a pseudoaneurysm of the ascending aorta. A unique feature of Triplex grafts is that they may reduce post-operative inflammation reaction; however, they could also invite a limited adhesion formation with the surrounding tissue, which contribute to prostheses elongation, due to a lack of prostheses stability and fixation. A careful observation based on the feature of implanted protheses is required.

    DOI: 10.1093/jscr/rjac255

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  • Case of rapid aortic remodeling after thoracic endovascular aortic repair for retrograde type A aortic dissection. 国際誌

    Yuta Kikuchi, Masahiro Tsutsui, Kohei Ishido, Masahiko Narita, Ryohei Ushioda, Tomonori Shirasaka, Natsuya Ishikawa, Hiroyuki Kamiya

    Journal of surgical case reports   2022 ( 3 )   rjac050   2022年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The proper surgical strategy for retrograde type A aortic dissection (RTAD) is still controversial, and some studies have reported the efficacy of frozen elephant trunk and thoracic endovascular aortic repair (TEVAR). A 68-year-old man was diagnosed with acute type A aortic dissection using enhanced computed tomography. The false lumen at the arch and ascending aorta was thrombosed, and the primary entry was placed in the descending aorta. In addition, there were malperfusions of the right renal artery and both iliac arteries. We performed TEVAR using the right femoral artery combined with the petticoat technique. At 11 days postoperatively, we observed rapid aortic remodeling at the arch and ascending aorta. The patient was discharged uneventfully after 14 days. We believe that TEVAR for RTAD is effective in appropriate patients. However, the accumulation of the number of cases and accurate strategies for patient selection are in demand.

    DOI: 10.1093/jscr/rjac050

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  • The David Operation Offers Shorter Hemostasis Time Than the Bentall in Case of Acute Aortic Dissection Type A. 国際誌

    Ryohei Ushioda, Tomonori Shirasaka, Taro Kanamori, Atsuko Fujii, Makoto Shirakawa, Taro Takeuchi, Hiroyuki Kamiya

    Cureus   14 ( 1 )   e21747   2022年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background The aim of the present study was to compare the clinical outcome of the David operation and the Bentall operation in patients with Stanford type A acute aortic dissection (AADA) from the viewpoint of hemostasis. Methods Between April 2016 and April 2020, 235 patients underwent emergent surgery for AADA. Of them, 38 patients required aortic root replacement (ARR: The David operation 17, the Bentall operation 21). The mean age was 59.3±12.6 years. In the present series, the David operation was the first choice for relatively young people, and the Bentall operation was performed for relatively elderly patients and cases in which valve-sparing seemed impossible. Results Between the David and the Bentall group, the 30-day mortality rate did not differ significantly. However, hemostasis time (144.6±50.3 vs. 212.5±138.1 min, p=0.047), defined as the interval from the cessation of cardio-pulmonary bypass (CPB) to the end of the operation, and total operation time (477.8±85.7 vs. 578.3±173.6 min, p=0.027) were significantly shorter in the David group than in the Bentall group, and the amount of blood transfusion was less in the David group than in the Bentall group (red blood cells: 3.5±3.6 vs. 9.2±5.9 units, p=0.013; fresh frozen plasma: 4.1±4.7 vs 9.4±5.1 units, p=0.002; platelet concentrate: 33.2±11.3 vs 42.2±12.0 units, p=0.025). Conclusion David operation offers a shorter hemostasis time and consequently shorter operation time than the Bentall operation in the setting of AADA, probably due to double suture lines, despite its surgical complexity.

    DOI: 10.7759/cureus.21747

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  • Calcified amorphous tumor located on a severely calcified mitral annulus in a patient with normal renal function. 国際誌

    Ryohei Ushioda, Tomonori Shirasaka, Shinsuke Kikuchi, Hiroyuki Kamiya, Taro Kanamori

    Journal of surgical case reports   2022 ( 1 )   rjab608   2022年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A calcified amorphous tumor (CAT) of the heart is a rare, nonneoplastic, intracavitary cardiac mass. Histological examination shows that it contains calcified and amorphous fibrous material with underlying chronic inflammation. Surgical excision is generally recommended to avoid future embolism. The risk of embolism has been reported to be especially high in mitral-annular-calcification-related CAT, which constitutes a subgroup of CAT that is often associated with end-stage renal disease. A case of a CAT attached to the anterior annulus of the mitral valve that was easily removed with a light touch of the forceps through aortotomy is reported.

    DOI: 10.1093/jscr/rjab608

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  • Recombinant Activated Factor VII in Aortic Surgery for Patients Under Hypothermic Circulatory Arrest. 国際誌

    Hayato Ise, Ryohei Ushioda, Hirotsugu Kanda, Fumiaki Kimura, Yasuaki Saijo, Payam Akhyari, Artur Lichtenberg, Hiroyuki Kamiya

    Therapeutics and clinical risk management   18   337 - 348   2022年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: We aimed to identify the risk factors of critical bleeding and investigate the safety of recombinant activated factor VII (rFVIIa) in aortic surgery under hypothermic circulatory arrest (HCA). PATIENTS AND METHODS: The present single-center retrospective study compared the baseline characteristics of 144 patients who underwent aortic surgery under HCA at our institute. Among the total cohort of 144 patients, 42 received rFVIIa (rFVIIa group), while the remaining 102 patients did not (non-rFVIIa group). Perioperative bleeding and transfusions, postoperative 30-day mortality, and adverse events (AEs) were analyzed in 29 propensity score-matched pairs. RESULTS: Before surgery, the rFVIIa group demonstrated a greater number of shocks (p=0.019), higher JapanSCORE II mortality rate (p=0.033), low platelet count (p=0.015) and fibrinogen (p<0.001) level, prolonged activated partial thromboplastin time (aPTT) (p=0.005) and prothrombin time international normalized ratio (PT-INR) (p=0.006), and longer aortic cross clamp time (p=0.049). Postoperative bleeding, transfusion, 30-day mortality, and AEs were comparable between the groups both in the entire-unmatched cohort and propensity score matching cohort. CONCLUSION: Preoperative shock, higher JapanSCORE II mortality rates, low platelet and fibrinogen levels, prolonged aPTT and PT-INR, and longer aortic clamping time might be risk factors for excessive bleeding and indicate the need for rFVIIa treatment. The present study suggests that rFVIIa can be safely used to address critical and continuous bleeding in spite of adequate transfusion and supplementation of other coagulation factors in aortic surgery under HCA, without an increase in 30-day mortality and AEs.

    DOI: 10.2147/TCRM.S352609

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  • A case of TEVAR for acute aortic dissection after MICS AVR and retroperitoneal tumor resection. 国際誌

    Masahiro Tsutsui, Masahiko Narita, Ryohei Ushioda, Yuta Kikuchi, Tomonori Shirasaka, Natsuya Ishikawa, Hiroyuki Kamiya

    Journal of surgical case reports   2021 ( 12 )   rjab559   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    If multiple treatments are performed within a short time, when something occurs, it is difficult to identify its cause. Here, we present a case of thoracic endovascular aortic repair (TEVAR) for acute aortic dissection (AAD) after multiple treatments. A 76-year-old woman underwent minimally invasive aortic valve replacement, transcatheter lumbar artery embolism and retroperitoneal tumor resection within a short period of time. After a series of procedures, the patient experienced sudden back pain, and computed tomography revealed an AAD Type B. Her back pain persisted; therefore, we performed TEVAR, and the post-operative course was uneventful. In this case, the relationship between AAD and treatment before AAD was unclear, but AAD should considered when performing treatments that may cause AAD.

    DOI: 10.1093/jscr/rjab559

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  • Feasibility of the AtriClip Pro Left Atrium Appendage Elimination Device via the Transverse Sinus in Minimally Invasive Mitral Valve Surgery. 国際誌

    Tomonori Shirasaka, Shingo Kunioka, Masahiko Narita, Ryohei Ushioda, Keisuke Shibagaki, Yuta Kikuchi, Naohiro Wakabayashi, Natsuya Ishikawa, Hiroyuki Kamiya

    Journal of chest surgery   54 ( 5 )   383 - 388   2021年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Achieving external access to and manual occlusion of the left atrial appendage (LAA) during minimally invasive mitral valve surgery (MIMVS) through a small right thoracotomy is difficult. Occlusion of the LAA using an epicardial closure device seems quite useful compared to other surgical techniques. METHODS: Fourteen patients with atrial fibrillation underwent MIMVS with concomitant surgical occlusion of the LAA using double-layered endocardial closure stitches (n=6, endocardial suture group) or the AtriClip Pro closure device (n=8, AtriClip group) at our institution. The primary safety endpoint was any device-related adverse event, and the primary efficacy endpoint was successful complete occlusion of blood flow into the LAA as assessed by transthoracic echocardiography at hospital discharge. The primary efficacy endpoint for stroke reduction was the occurrence of ischemic or hemorrhagic neurologic events. RESULTS: All patients underwent LAA occlusion as scheduled. The cardiopulmonary bypass and aortic cross-clamp times in the endocardial suture group and the AtriClip group were 202±39 and 128±41 minutes, and 213±53 and 136±44 minutes, respectively (p=0.68, p=0.73). No patients in either group experienced any device-related serious adverse events, incomplete LAA occlusion, early postoperative stroke, or neurologic complication. CONCLUSION: Epicardial LAA occlusion using the AtriClip Pro during MIMVS in patients with mitral valve disease and atrial fibrillation is a simple, safe, and effective adjunctive procedure.

    DOI: 10.5090/jcs.21.048

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  • Ventricular septal perforation followed by papillary muscle rupture with acute myocardial infarction: efficacy of venoarterial extracorporeal membrane oxygenation. 国際誌

    Ryohei Ushioda, Atsuko Fujii, Makoto Shirakawa, Tomonori Shirasaka, Shinsuke Kikuchi, Hiroyuki Kamiya, Taro Kanamori

    Journal of surgical case reports   2020 ( 7 )   rjaa188   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The occurrence of multiple mechanical complications after myocardial infarction in the same patient may be extremely rare, and the surgical strategy may be very complex because each mechanical complication can be extremely fatal. The case of a patient who underwent repair of a ventricular septal perforation by venoarterial extracorporeal membrane oxygenation (VA-ECMO), then mitral valve replacement and VA-ECMO for papillary muscle rupture 2 weeks after the ventricular septal perforation repair, is reported. Immediate preoperative stabilization with VA-ECMO may play a crucial role in treating multiple mechanical complications after myocardial infarction.

    DOI: 10.1093/jscr/rjaa188

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  • Examination of Anterior Leaflet Pseudoprolapse Causing Severe Mitral Regurgitation and Its Ideal Surgical Procedure. 国際誌

    Chiharu Tanaka, Kazumi Akasaka, Ryohei Ushioda, Tomoki Nakatsu, Naohiro Wakabayashi, Hayato Ise, Hiroto Kitahara, Sentaro Nakanishi, Natsuya Ishikawa, Hiroyuki Kamiya

    The heart surgery forum   23 ( 2 )   E205-E211   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: The aim of this study is to evaluate severe mitral regurgitation caused by so called atrial leaflet "pseudoprolapse" and verify the effect of simple annular stabilization. METHODS: One-hundred-twenty-two patients underwent surgery for severe mitral regurgitation at our institute between January 2015 to July 2018. Of those, 32 cases diagnosed as anterior leaflet prolapse that underwent mitral repair were analyzed. Ten cases with pseudoprolapse, which is defined as anterior leaflet prolapse without dropping into the left atrium beyond the annular line causing eccentric regurgitation flow directed to the posterior atrium, were classified as the Pseudoprolapse Group. The other 22 cases had obvious anterior leaflet prolapse dropping into the left atrium; these cases were classified as the True Prolapse Group. We compared clinical findings between the 2 groups and reviewed pseudoprolapse cases. RESULTS: Patients in the Pseudoprolapse Group had lower ejection fraction and lower regurgitation volume than those in the True Prolapse Group. A2 lesion as main inflow of regurgitation was more included in the Pseudoprolapse Group. All but one patient in the Pseudoprolapse Group received only simple annuloplasty, and all patients in the True Prolapse Group received leaflet repair and annuloplasty. In both groups, mid-term regurgitation grade and the reoperation rate were satisfactory. In the Pseudoprolapse Group, 6 cases were clarified as atrial functional mitral regurgitation, and 4 cases were considered to have focal posterior leaflet tethering. CONCLUSIONS: Pseudoprolapse cases could be characterized by low ejection fraction, low regurgitation volume, and A2 prolapse. For most cases with pseudoprolapse, simple annuloplasty may be enough, however further study is needed.

    DOI: 10.1532/hsf.2895

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  • Ventricular septal perforation followed by papillary muscle rupture with acute myocardial infarction: Efficacy of veno-arterial extracorporeal membrane oxygenation 査読

    Ryohei Ushioda, Atsuko Fujii, Makoto Shirakawa, Tomonori Shirasaka, Shinsuke Kikuchi, Hiroyuki Kamiya, Taro Kanamori

    Journal of surgical case reports   2020年4月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • [Surgical Treatment for Postoperative Chylothorax after Aortic Arch Replacement via Median Sternotomy].

    Taro Kanamori, Makoto Shirakawa, Tomonori Shirasaka, Taro Takeuchi, Atsuko Fujii, Ryohei Ushioda, Keiko Ueda

    Kyobu geka. The Japanese journal of thoracic surgery   73 ( 3 )   197 - 201   2020年3月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    Postoperative chylothorax is known as a possible complication after thoracic surgery, but no treatment strategy has been established. We report a case of successful surgical treatment for postoperative chylothorax after redo aortic arch replacement via median sternotomy. A 48-year-old man, who had undergone redo aortic arch replacement for aortic pseudoaneurysm due to prosthetic vascular graft infection, developed postoperative chylothorax. Despite the conservative treatment with fasting and administration of octreotide for 4 days, there was no effect on reduction in drainage. Surgical repair was performed on postoperative day 13. About 3 hours before surgery, milk was administered from the nasogastric tube to make the drainage milky. After median re-sternotomy, a stump of the thoracic duct was clearly identified and exposed in the posterior mediastinum, and the thoracic duct was easily closed by clipping. There was no recurrence of chylothorax and oral intake was re-started on day 2. Early operation might be effective against postoperative chylothorax.

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  • A case of pulmonary artery sarcoma that was initially mis-diagnosed as pulmonary embolism. 国際誌

    Ryohei Ushioda, Hiroto Kitahara, Hayato Ise, Yuta Koichi, Naohiro Wakabayashi, Chiharu Tanaka, Sentaro Nakanishi, Natsuya Ishikawa, Hiroyuki Kamiya

    Journal of surgical case reports   2019 ( 3 )   rjz078   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Pulmonary artery sarcoma is a rare and highly malignant neoplasm. Early diagnosis and a multidisciplinary approach including surgical treatment and optimal medical therapy could prolong survival. Since the clinical symptoms and imaging findings of pulmonary artery sarcoma mimic pulmonary embolism, definitive diagnosis and surgical intervention are often delayed. In this report, a case of pulmonary artery sarcoma that was initially misdiagnosed as pulmonary embolism is presented.

    DOI: 10.1093/jscr/rjz078

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▼全件表示

共同研究・競争的資金等の研究課題

  • 冠動脈バイパス術のグラフト材としての生体吸収性ナノファイバーグラフトの研究

    研究課題/領域番号:23K24413  2022年4月 - 2026年3月

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    紙谷 寛之, 小山 恭平, 筒井 真博, 吉田 巧, 広藤 愛菜, 潮田 亮平, 白坂 知識, 成田 昌彦, 菊池 悠太

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    配分額:17,290,000円 ( 直接経費:13,300,000円 、 間接経費:3,990,000円 )

    冠動脈バイパス術や下肢血行再建術に使用可能な直径4㎜以下の細径人工血管の開発は心臓血管外科学分野における長年の課題である。我々は、生体内に移植することでスキャフォ
    ルド内に自己組織が生着し長期開存が得られる代用血管を開発するため、生体吸収性ナノファイバー(NF)に着目した。現在までに、血小板吸着抑制作用を発揮する親水化加工NFグラフトを開発し、ラット腹部大動脈置換モデルにおいて移植後グラフト全長にわたり内皮細胞が生着することを証明し、最長8か月におよぶ長期開存性を確認した。本研究では、臨床での実用化を目的として、完全自家組織化を可能にする分解性スキャフォールドの最適化を行い、小動物モデルから中大動物モデルを用いて研究コンセプトの証明と、臨床での使用に耐えうるハンドリング性能の評価を行う。
    2022年度は、生体内で完全吸収されるスキャフォールドを作成するための基礎データの取得を行った。これまで研究に用いてきたポリカプロラクタン(PCL)は、生体内での吸収が非常に遅いため、分解性が高いポリグリコール酸(PGA)やポリ乳酸(PLA)と混合して分解性をコントロールする。これまで各素材の生体内分解速度に関する定量的なデータが存在しないため、その定量評価を行った。皮下環境下での評価はほぼ終えて、ラットを用いて血管環境下での評価を始めている。並行して、グラフトの分解による完全自家組織化を評価するためには長期観察が必要なため、長期飼育可能なウサギでの評価系の確立を行った。このモデルを用いて、PCLグラフトが長期的にどのような形態変化を示すか検討を始めた。

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  • 心筋再生を可能にする心筋細胞の分裂促進因子の同定と機能解析

    研究課題/領域番号:21K20939  2021年8月 - 2023年3月

    日本学術振興会  科学研究費助成事業  研究活動スタート支援

    潮田 亮平

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    配分額:3,120,000円 ( 直接経費:2,400,000円 、 間接経費:720,000円 )

    終末分化した心筋細胞は分裂能力が著しく低いため、心臓は再生能力を持っていないがその原因は十分に理解されていない。本研究では、RNA結合タンパク質Csdc2が、細胞分化や分裂停止に関わるかどうかについて、マウスの組織や培養細胞モデルを用いて検討を行った。成体マウスにおいて、心臓や脳など細胞が分裂しない組織でCsdc2の遺伝子発現が高かった。心筋細胞では、分裂活性が高い胎児期と比較して分裂を停止した成熟心筋細胞でCsdc2の発現が上昇していた。C2C12細胞において、Csdc2は骨格筋分化に伴い発現が上昇した。これらの結果から、Csdc2は分化や細胞周期の逸脱に関与していることが示唆される。

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