2026/03/24 更新

写真a

ソン　ガンホウ
孫　雁鵬
YANPENG SUN
所属
医学部 医学科 共同研究講座 先進ゲノム地域医療講座
外部リンク

学位

  • 博士(ソフトマター科学) （ 2025年3月   北海道大学 ）

研究キーワード

  • がん幹細胞

  • 腫瘍微小環境

  • ソフトマター

  • 腫瘍生物学

研究分野

  • ライフサイエンス / 腫瘍生物学

  • ライフサイエンス / 実験病理学

論文

  • Six-Color Multiplex Digital PCR Assays for Comprehensive Screening and Identification of Multiple Driver Mutations Associated with Pancreatic Carcinogenesis

    Chiho Maeda, Yusuke Ono, Kenji Takahashi, Miyuki Mori, Mayumi Suzuki, Nobue Tamamura, Yanpeng Sun, Taito Itoh, Hiroki Tanaka, Hidemasa Kawabata, Tetsuhiro Okada, Kazuya Koyama, Yu Ohtaki, Yuko Omori, Takuya Yamamoto, Yusuke Mizukami

    Clinical Chemistry   2026年1月

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    掲載種別：研究論文（学術雑誌）   出版者・発行元：Oxford University Press (OUP)  

    Abstract

    Background

    Digital polymerase chain reaction (dPCR) is widely recognized for its high sensitivity in detecting low-frequency variants; however, conventional 2-color systems have limited multiplex capacity. Expanding this capability is essential for simultaneous detection of multiple driver mutations in cancer-related genes. KRAS and GNAS are key driver genes in the early development of pancreatic cancer and its precursor lesions, and mutations in these genes are often present at low abundance in clinical samples.

    Methods

    Two 6-color dPCR assays were developed using a droplet-based platform. PlexScreen-dPCR is a multicolored drop-off assay designed to screen for mutations in KRAS codons 12/13 and 61 and GNAS codon 201, without specifying individual variants. PlexID-dPCR employs variant-specific probes to distinguish among 14 predefined KRAS and GNAS mutations in a single reaction. The assays were validated using synthetic DNA, cell lines, 23 tissue samples, and 12 duodenal fluid samples. Customized primer/probe sets with 6 fluorophores were employed in a 6-color droplet dPCR system, and the limits of detection (LOD) were determined.

    Results

    PlexScreen-dPCR, applied in contrived samples, demonstrated LODs as low as 0.03% to 0.06%, enabling high-sensitivity detection of low-abundance mutations. PlexID-dPCR accurately identified all 14 variants in a single well. Both assays showed complete concordance with conventional methods, exhibiting a strong correlation for variant allele frequency quantification.

    Conclusions

    These 6-color dPCR assays offer scalable solutions for improved throughput detection of KRAS and GNAS mutations. Their compatibility with commercially available platforms and streamlined workflow support their integration into clinical practice. Further optimization can enhance cluster interpretation in high-plex settings and facilitate expansion toward broader genomic targets.

    DOI： 10.1093/clinchem/hvaf181

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  • Mechanochemistry-Induced Universal Hydrogel Surface Modification for Orientation and Enhanced Differentiation of Skeletal Muscle Myoblasts

    Yuheng Nie, Qifeng Mu, Yanpeng Sun, Zannatul Ferdous, Lei Wang, Cewen Chen, Tasuku Nakajima, Jian Ping Gong, Shinya Tanaka, Masumi Tsuda

    ACS Applied Bio Materials   2025年4月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1021/acsabm.4c01991

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  • Tumor-mimetic hydrogel stiffness regulates cancer stemness properties in H-Ras-transformed cancer model cells

    Yanpeng Sun, Yuheng Nie, Lei Wang, Jian Ping Gong, Shinya Tanaka, Masumi Tsuda

    Biochemical and Biophysical Research Communications   2025年1月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.bbrc.2024.151163

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