Updated on 2025/08/06

写真a

 
YAMASAKI Kazuo
 
Organization
School of Medicine Medical Course Basic Medicine Biochemistry
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Degree

  • 博士(理学) ( 1992.3   大阪大学 )

  • 理学修士 ( 1989.3   大阪大学 )

Research Interests

  • protein structure-function relationship

  • チロシン

  • カルシウムイオン

  • ATP結合部位

  • プロテアソーム

  • 生体エネルギー変換

  • 生体エネルギー転換

  • テトラニトロメタン

  • 疎水的相互作用

  • SERCAla

  • イオン輸送

  • 部位特異的変異

  • 筋小胞体

  • Ca^<2+>輸送

  • リン酸化中間体

  • カルシウムポンプ

  • 酵素反応速度論

  • P-type ATPase

  • 静電的相互作用

  • SERCA1a

  • エネルギー共役

  • 機能構造連関

  • 酵素反応

  • 小胞体

  • FDNP-ATP

  • COS-1

  • 酵素速度反応論

  • Nanodisc

  • Ca^<2+>ポンプ

  • カルシウムATPアーゼ

  • Ca2+-ATPase

Research Areas

  • Life Science / Functional biochemistry

  • Life Science / Structural biochemistry

  • Life Science / Biophysics

Education

  • Osaka University   Graduate School, Division of Natural Science

    - 1992.3

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    Country: Japan

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  • Osaka University   Faculty of Science   Depertment of Biology

    - 1992.3

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    Country: Japan

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  • Osaka University

    - 1992

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    Country: Japan

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  • Osaka University   Graduate School, Division of Natural Science

    - 1992

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  • Osaka University   School of Science

    - 1987

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    Country: Japan

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  • Osaka University   Faculty of Science

    - 1987

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Research History

  • Asahikawa Medical College   School of Medicine   Lecturer

    2015

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  • Asahikawa Medical College   Lecturer

    2011.4

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  • Asahikawa Medical College   Assistant Professor

    1992.4 - 2011.3

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Professional Memberships

Papers

  • Electrostatic interactions between single arginine and phospholipids modulate physiological properties of sarcoplasmic reticulum Ca2+-ATPase Reviewed International journal

    Kazuo Yamasaki, Takashi Daiho, Satoshi Yasuda, Stefania Danko, Jun-ichi Kawabe, Hiroshi Suzuki

    Scientific Reports   12 ( 1 )   12200 - 12200   2022.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    Arg324 of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase forms electrostatic interactions with the phosphate moiety of phospholipids in most reaction states, and a hydrogen bond with Tyr122 in other states. Using site-directed mutagenesis, we explored the functional roles of Arg324 interactions, especially those with lipids, which at first glance might seem too weak to modulate the function of such a large membrane protein. The hydrogen bond forms transiently and facilitates Ca<sup>2+</sup> binding from the cytoplasmic side. The contributions of the electrostatic interactions to the reaction steps were quantified using a rate vs activity coefficient plot. We found that the interaction between Arg324 and lipids decreases the affinity for luminal Ca<sup>2+</sup>. The transformation rate of the phosphoenzyme intermediate is facilitated by the electrostatic interactions, and the function of these interactions depends not only on the type but also on the composition of the phospholipids. The properties observed in microsomes could not be reproduced with any single phospholipid, but with a mixture of phospholipids that mimics the native membrane. These results suggest the importance of swapping of the lipid partners of different headgroups in the reaction step. This study shows that Arg324 plays a role in the reaction cycle via complex intra-protein and protein-lipid interactions.

    DOI: 10.1038/s41598-022-16091-9

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    Other Link: https://www.nature.com/articles/s41598-022-16091-9

  • Angle change of the A-domain in a single SERCA1a molecule detected by defocused orientation imaging Reviewed International journal

    加藤孝信, 大保貴嗣, 山崎和生, DANKO Stefania, 藤村章子, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   47th (CD-ROM) ( 1 )   13672 - 13672   2021.7

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    The sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) transports Ca2+ ions across the membrane coupled with ATP hydrolysis. Crystal structures of ligand-stabilized molecules indicate that the movement of actuator (A) domain plays a crucial role in Ca2+ translocation. However, the actual structural movements during the transitions between intermediates remain uncertain, in particular, the structure of E2PCa2 has not been solved. Here, the angle of the A-domain was measured by defocused orientation imaging using isotropic total internal reflection fluorescence microscopy. A single SERCA1a molecule, labeled with fluorophore ReAsH on the A-domain in fixed orientation, was embedded in a nanodisc, and stabilized on Ni-NTA glass. Activation with ATP and Ca2+ caused angle changes of the fluorophore and therefore the A-domain, motions lost by inhibitor, thapsigargin. Our high-speed set-up captured the motion during EP isomerization, and suggests that the A-domain rapidly rotates back and forth from an E1PCa2 position to a position close to the E2P state. This is the first report of the detection in the movement of the A-domain as an angle change. Our method provides a powerful tool to investigate the conformational change of a membrane protein in real-time.

    DOI: 10.1038/s41598-021-92986-3

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  • Nanodisc-based kinetic assays reveal distinct effects of phospholipid headgroups on the phosphoenzyme transition of sarcoplasmic reticulum Ca2+-ATPase Reviewed International journal

    Kazuo Yamasaki, Takashi Daiho, Stefania Danko, Satoshi Yasuda, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   292 ( 49 )   20218 - 20227   2017.12

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    DOI: 10.1074/jbc.M117.816702

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  • Membrane Perturbation of ADP-insensitive Phosphoenzyme of Ca2+-ATPase Modifies Gathering of Transmembrane Helix M2 with Cytoplasmic Domains and Luminal Gating Reviewed International journal

    Stefania Danko, Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki

    SCIENTIFIC REPORTS   7   41172 - 41172   2017.1

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    DOI: 10.1038/srep41172

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  • Glycine 105 as Pivot for a Critical Knee-like Joint between Cytoplasmic and Transmembrane Segments of the Second Transmembrane Helix in Ca2+-ATPase Reviewed International journal

    Takashi Daiho, Kazuo Yamasaki, Stefania Danko, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   291 ( 47 )   24688 - 24701   2016.11

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    DOI: 10.1074/jbc.M116.759704

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  • Assembly of a Tyr(122) Hydrophobic Cluster in Sarcoplasmic Reticulum Ca2+-ATPase Synchronizes Ca2+ Affinity Reduction and Release with Phosphoenzyme Isomerization Reviewed International journal

    Kazuo Yamasaki, Takashi Daiho, Stefania Danko, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   290 ( 46 )   27868 - 27879   2015.11

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    DOI: 10.1074/jbc.M115.693770

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  • Second Transmembrane Helix (M2) and Long Range Coupling in Ca2+-ATPase Reviewed International journal

    Takashi Daiho, Kazuo Yamasaki, Stefania Danko, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   289 ( 45 )   31241 - 31252   2014.11

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    DOI: 10.1074/jbc.M114.584086

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  • Some Amino Acid Residues on L78 Affect Ca2+ Transport of SERCA1a Reviewed

    Wang Guo-Li, Takashi Daiho, Kazuo Yamasaki, Stefania Danko, Wang Biao, Su Wen-Hui, Hiroshi Suzuki

    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS   41 ( 8 )   796 - 803   2014.8

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    Language:Chinese   Publishing type:Research paper (scientific journal)  

    DOI: 10.3724/SP.J.1206.2013.00524

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  • 1P111 Second Transmembrane Helix (M2) and Long-range Coupling in Ca^<2+>-ATPase(03. Membrane proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Daiho Takashi, Yamasaki Kazuo, Danko Stefania, Suzuki Hiroshi

    Seibutsu Butsuri   54 ( 1 )   S159   2014

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    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.54.S159_3

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  • Roles of Long-range Electrostatic Domain Interactions and K+ in Phosphoenzyme Transition of Ca2+-ATPase Reviewed International journal

    Kazuo Yamasaki, Takashi Daiho, Stefania Danko, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   288 ( 28 )   20646 - 20657   2013.7

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    DOI: 10.1074/jbc.M113.482711

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  • Ca2+ Release to Lumen from ADP-sensitive Phosphoenzyme E1PCa(2) without Bound K+ of Sarcoplasmic Reticulum Ca2+-ATPase Reviewed International journal

    Kazuo Yamasaki, Takashi Daiho, Stefania Danko, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   285 ( 49 )   38674 - 38683   2010.12

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    DOI: 10.1074/jbc.M110.183343

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  • Stable Structural Analog of Ca2+-ATPase ADP-insensitive Phosphoenzyme with Occluded Ca2+ Formed by Elongation of A-domain/M1 '-linker and Beryllium Fluoride Binding Reviewed International journal

    Takashi Daiho, Stefania Danko, Kazuo Yamasaki, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   285 ( 32 )   24538 - 24547   2010.8

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    DOI: 10.1074/jbc.M110.144535

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  • 筋小胞体Ca2+-ATPaseのP-N hingeに位置する静電的相互作用の役割についての研究

    山崎 和生

    旭川医科大学研究フォーラム   10 ( 1 )   79 - 80   2010.2

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    Other Link: http://search.jamas.or.jp/link/ui/2010188382

  • Mechanism of Ca<sup>2+</sup> Pump as Revealed by Mutations, Development of Stable Analogs of Phosphorylated Intermediates, and Their Structural Analyses Reviewed

    鈴木裕, 山崎和生, 大保貴嗣, DANKO Stefania

    薬学雑誌   130 ( 2 )   179 - 189   2010.2

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    DOI: 10.1248/yakushi.130.179

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  • Roles of Interaction between Actuator and Nucleotide Binding Domains of Sarco(endo)plasmic Reticulum Ca2+-ATPase as Revealed by Single and Swap Mutational Analyses of Serine 186 and Glutamate 439 Reviewed International journal

    Xiaoyu Liu, Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   284 ( 37 )   25190 - 25198   2009.9

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    DOI: 10.1074/jbc.M109.034140

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  • Formation of the Stable Structural Analog of ADP-sensitive Phosphoenzyme of Ca2+-ATPase with Occluded Ca2+ by Beryllium Fluoride STRUCTURAL CHANGES DURING PHOSPHORYLATION AND ISOMERIZATION Reviewed International journal

    Stefania Danko, Takashi Daiho, Kazuo Yamasaki, Xiaoyu Liu, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   284 ( 34 )   22722 - 22735   2009.8

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    DOI: 10.1074/jbc.M109.029702

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  • Roles of Tyr(122)-hydrophobic Cluster and K+ Binding in Ca2+-releasing Process of ADP-insensitive Phosphoenzyme of Sarcoplasmic Reticulum Ca2(+)-ATPase Reviewed International journal

    Kazuo Yamasaki, Guoli Wang, Takashi Daiho, Stefania Danko, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   283 ( 43 )   29144 - 29155   2008.10

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    DOI: 10.1074/jbc.M804596200

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  • Critical role of glu(40)-ser(48) loop linking actuator domain and first transmembrane helix of Ca2+-ATPase in Ca2+ deocclusion and release from ADP-insensitive phosphoenzyme Reviewed International journal

    Takashi Daiho, Kazuo Yamasaki, Stefania Danko, Hiroshi Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 47 )   34429 - 34447   2007.11

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    DOI: 10.1074/jbc.M707665200

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  • Comprehensive analysis of expression and function of 51 sarco(endo) plasmic reticulum Ca2+-ATPase mutants associated with Darier disease Reviewed International journal

    Yuki Miyauchi, Takashi Daiho, Kazuo Yamasaki, Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Stefania Danko, Hiroshi Suzuki, Hajime Iizuka

    JOURNAL OF BIOLOGICAL CHEMISTRY   281 ( 32 )   22882 - 22895   2006.8

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    DOI: 10.1074/jbc.M601966200

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  • ATP2C1 is specifically localized in the basal layer of normal epidermis and its depletion triggers keratinocyte differentiation Reviewed International journal

    M Yoshida, K Yamasaki, T Daiho, H Iizuka, H Suzuki

    JOURNAL OF DERMATOLOGICAL SCIENCE   43 ( 1 )   21 - 33   2006.7

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    DOI: 10.1016/j.jdermsci.2006.03.003

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  • Critical hydrophobic interactions between phosphorylation and actuator domains of Ca2+-ATPase for hydrolysis of phosphorylated intermediate Reviewed International journal

    GL Wang, K Yamasaki, T Daiho, H Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   280 ( 28 )   26508 - 26516   2005.7

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    DOI: 10.1074/jbc.M503789200

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  • 1P224 Stable analogs for phosphoenzyme intermediates of sarcoplasmic reticulum Ca-ATPase

    Danko S., Yamasaki K., Daiho T., Suzuki H.

    Seibutsu Butsuri   45   S87   2005

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    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.45.S87_4

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  • Distinct types of abnormality in kinetic properties of three Darier disease-causing sarco(endo)plasmic reticulum Ca2+-ATPase mutants that exhibit normal expression and high Ca2+ transport activity Reviewed International journal

    K Sato, K Yamasaki, T Daiho, Y Miyauchi, H Takahashi, A Ishida-Yamamoto, S Nakamura, H Iizuka, H Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   279 ( 34 )   35595 - 35603   2004.8

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    DOI: 10.1074/jbc.M404887200

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  • Distinct natures of beryllium fluoride-bound, aluminum fluoride-bound, and magnesium fluoride-bound stable analogues of an ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+-ATPase - Changes in catalytic and transport sites during phosphoenzyme hydrolysis Reviewed International journal

    S Danko, K Yamasaki, T Daiho, H Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   279 ( 15 )   14991 - 14998   2004.4

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    DOI: 10.1074/jbc.M313363200

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  • Multiple and distinct effects of mutations of Tyr(122), Glu(123), Arg(324), and Arg(334) involved in interactions between the top part of second and fourth transmembrane helices in sarcoplasmic reticulum Ca2+-ATPase - Changes in cytoplasmic domain organization during isometric transition of phosphoenzyme intermediate and subsequent Ca2+ release Reviewed International journal

    K Yamasaki, T Daiho, S Danko, H Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   279 ( 3 )   2202 - 2210   2004.1

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    DOI: 10.1074/jbc.M309398200

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  • Deletions of any single residues in Glu(40)-Ser(48) loop connecting a domain and the first transmembrane helix of sarcoplasmic reticulum Ca(2+)-ATPase result in almost complete inhibition of conformational transition and hydrolysis of phosphoenzyme intermediate Reviewed International journal

    T Daiho, K Yamasaki, GL Wang, S Danko, H Iizuka, H Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   278 ( 40 )   39197 - 39204   2003.10

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    DOI: 10.1074/jbc.M305200200

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  • Val(200) residue in Lys(189)-Lys(205) outermost loop on the A domain of sarcoplasmic reticulum Ca2+-ATPase is critical for rapid processing of phosphoenzyme intermediate after loss of ADP sensitivity Reviewed International journal

    S Kato, M Kamidochi, T Daiho, K Yamasaki, W Gouli, H Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   278 ( 11 )   9624 - 9629   2003.3

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    DOI: 10.1074/jbc.M208861200

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  • Remarkable stability of solubilized and delipidated sarcoplasmic reticulum Ca2+-ATPase with tightly bound fluoride and magnesium against detergent-induced denaturation

    K Yamasaki, T Daiho, H Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   277 ( 16 )   13615 - 13619   2002.4

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    DOI: 10.1074/jbc.M200625200

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  • Organization of cytoplasmic domains of sarcoplasmic reticulum Ca(2+)-ATPase in E(1)P and E(1)ATP states: a limited proteolysis study Reviewed

    S Danko, K Yamasaki, T Daiho, H Suzuki, C Toyoshima

    FEBS LETTERS   505 ( 1 )   129 - 135   2001.9

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  • Mutations of either or both Cys(876) and Cys(888) residues of sarcoplasmic reticulum Ca2+-ATPase result in a complete loss of Ca2+ transport activity without a loss of Ca2+-dependent ATPase activity - Role of the Cys(876)-Cys(888) disulfide bond

    T Daiho, K Yamasaki, T Saino, M Kamidochi, K Satoh, H Iizuka, H Suzuki

    JOURNAL OF BIOLOGICAL CHEMISTRY   276 ( 35 )   32771 - 32778   2001.8

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    DOI: 10.1074/jbc.M101229200

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  • Deletions or specific substitutions of a few residues in the NH2-terminal Ala3-Thr9 region of sarcoplasmic reticulum Ca2+-ATPase cause inactivation and rapid degradation of the enzyme expressed in COS-1 cells

    T Daiho, K Yamasaki, H Suzuki, T Saino, T Kanazawa

    NA/K-ATPASE AND RELATED ATPASES   1207   293 - 296   2000

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  • Only half of the Ca2+-ATPase molecules present in sarcoplasmic reticulum vesicles can be phosphorylated with ATP or inorganic phosphate Reviewed

    H Suzuki, T Daiho, K Yamasaki, T Kanazawa

    NA/K-ATPASE AND RELATED ATPASES   1207   381 - 388   2000

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  • Modification of histidine 5 in sarcoplasmic reticulum Ca2+-ATPase by diethyl pyrocarbonate causes strong inhibition of formation of the phosphoenzyme intermediate from inorganic phosphate

    K Yamasaki, T Daiho, T Saino, T Kanazawa

    JOURNAL OF BIOLOGICAL CHEMISTRY   272 ( 49 )   30627 - 30636   1997.12

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    DOI: 10.1074/jbc.272.49.30627

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  • Identification of arginyl residues located at the ATP binding site of sarcoplasmic reticulum Ca2+-ATPase - Modification with 1,2-cyclohexanedione Reviewed

    K Kimura, H Suzuki, T Daiho, K Yamasaki, T Kanazawa

    JOURNAL OF BIOLOGICAL CHEMISTRY   271 ( 46 )   28933 - 28941   1996.11

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  • 3'-O-(5-FLUORO-2,4-DINITROPHENYL)-ATP EXCLUSIVELY LABELS LYS-492 AT THE ACTIVE-SITE OF THE SARCOPLASMIC-RETICULUM CA2+-ATPASE

    K YAMASAKI, T DAIHO, T KANAZAWA

    JOURNAL OF BIOLOGICAL CHEMISTRY   269 ( 6 )   4129 - 4134   1994.2

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  • 筋小体Ca-ATPaseの高および低親和性部位へのvanadate結合によるリン酸側からのリン酸化酵素形成の阻害

    山崎 和生, 山本 泰望

    The Journal of Biochemistry   112 ( 5 )   658 - 664   1992.11

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  • 骨格筋筋小胞体(SA) Ca-ATPaseにおける2種類のvanadate結合部位の存在

    山崎 和生, 山本 泰望

    The Journal of Biochemistry   110 ( 6 )   915 - 921   1991.12

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  • N,N′-(1,4-phenylene)bismaleimideによる筋小胞体Ca2+-ATPase分子間架橋へのadenyl-5′-imdodiphosphateとvanadateの効果

    山崎 和生, 山本 泰望

    The Journal of Biochemistry   106 ( 6 )   1114 - 1120   1989.12

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Books

  • Only half of the Ca<sup>2+</sup>-ATPase molecules present in sarcoplasmic reticulum vesicles can be phosphorylated with ATP or inorganic phosphate(jointly worked)

    "Na/K-ATPase and Related ATPases"Elsevier Science B.V.(Netherland)  2000 

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  • Deletion on specific substitutions of a few residues in the NH<sub>2</sub>-terminal Ala3-Thr9 region of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase cause inactivation and rapid degradation of the enzyme expressed in COS-1 cells(jointly worked)

    "Na/K-ATPase and Related ATPases"Elsevier Science B.V.(Netherland)  2000 

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MISC

  • Effects of lipid environments on EP formation steps of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase.

    山崎和生, 安田哲, 大保貴嗣, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   49th   2023

  • Functional roles of A-domain in energy coupling of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase

    大保貴嗣, 中島恵一, ADAMO Hugo P., 山崎和生, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   49th   2023

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのリン酸化中間体形成過程に対する脂質の違いによる影響

    山崎和生, 安田哲, 大保貴嗣, 川辺淳一

    日本生化学会大会(Web)   96th   2023

  • 筋小胞体CaポンプのヘリックスM2/M6/M4のCa輸送における役割

    大保貴嗣, 山崎和生, 川辺淳一

    日本生化学会大会(Web)   96th   2023

  • Effects of mixed lipid environment on the enzymatic properties of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase

    山崎和生, 安田哲, 大保貴嗣, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   48th   2022

  • 筋小胞体CaポンプのヘリックスM2/M6のCa輸送における役割

    大保貴嗣, 山崎和生, 安田哲, 川辺淳一

    日本生化学会大会(Web)   95th   2022

  • Functional roles of interaction of M2/M6 helix in energy coupling of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase

    大保貴嗣, 山崎和生, 安田哲, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   48th   2022

  • 混合脂質環境における筋小胞体Ca<sup>2+</sup>-ATPaseの特性の変化について

    山崎和生, 安田哲, 大保貴嗣, 川辺淳一

    日本生化学会大会(Web)   95th   2022

  • Diversity of lipid-protein interaction mediated by multi-functional residue Arg<sup>324</sup> of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase.

    山崎和生, 大保貴嗣, 安田哲, DANKO Stefania, 川辺淳一, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   47th (CD-ROM)   2021

  • 筋小胞体カルシウムポンプの多機能側鎖Arg<sup>324</sup>と多種のリン脂質のアンサンブル

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 川辺淳一, 鈴木裕

    日本生化学会大会(Web)   94th   2021

  • 筋小胞体Ca<sup>2+</sup>ポンプのエネルギー共役:細胞質-膜貫通領域間における構造変化の伝達

    大保貴嗣, 山崎和生, 川辺淳一

    日本生化学会大会(Web)   94th   2021

  • Functional roles of membrane-spanning portion of M2 helix in energy coupling of Sarcoplasmic Reticulum Ca<sup>2+</sup>-ATPase

    大保貴嗣, 山崎和生, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   47th (CD-ROM)   2021

  • 筋小胞体カルシウムポンプの結晶構造中に見られたArg<sup>324</sup>とリン脂質間のリンクが果たす役割について

    山崎和生, 大保貴嗣, 安田哲, DANKO Stefania, 川辺淳一, 鈴木裕

    日本生化学会大会(Web)   93rd   2020

  • Functional roles of M2 membrane-spanning helix in energy coupling of Sarcoplasmic Reticulum Ca<sup>2+</sup>-ATPase

    大保貴嗣, 山崎和生, 安田哲, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   46th   2020

  • Functional roles of protein-lipid interaction between Arg<sup>324</sup> and phospholipids observed in crystal structures of sarcoplasmic reticulum Ca<sup>2+</sup>-pump

    山崎和生, 大保貴嗣, 安田哲, DANKO Stefania, 川辺淳一, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   46th   2020

  • 筋小胞体Ca<sup>2+</sup>ポンプのM2ヘリックス:膜貫通部分のエネルギー共役における役割

    大保貴嗣, 山崎和生, 安田哲, 川辺淳一

    日本生化学会大会(Web)   93rd   2020

  • Human flippase ATP8A1 reaction mechanism.

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, SHIN Hye-Won, DANKO Stefania, 川辺淳一, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   46th   2020

  • ヒトフリッパーゼATP8A1とCaポンプの反応機構の比較

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, 申惠媛, STEFANIA Danko, 川辺淳一, 鈴木裕

    日本生化学会大会(Web)   93rd   2020

  • Second Transmembrane Helix (M2) of Sarcoplasmic Reticulum Ca<sup>2+</sup>-ATPase; Functional roles of M2 membrane-spanning region in energy coupling

    大保貴嗣, 山崎和生, DANKO Stefania, 安田哲

    日本生体エネルギー研究会討論会講演要旨集   45th   2019

  • ヒトフリッパーゼATP8A1の反応機構における脂質の影響

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, 申惠媛, Danko Stefania, 鈴木裕

    日本生化学会大会(Web)   92nd   2019

  • 筋小胞体カルシウムポンプのTyr<sup>122</sup>クラスター変異体で観測された異常に遅いEP分解

    山崎和生, 市村祐人, 山崎誠一郎, 大保貴嗣, 安田哲, STEFANIA Danko, 鈴木裕

    日本生化学会大会(Web)   92nd   2019

  • 筋小胞体Ca2+ポンプの第2膜貫通ヘリックス:膜貫通部分のエネルギー共役における役割

    大保貴嗣, 山崎和生, 安田哲, STEFANIA Danko

    日本生化学会大会(Web)   92nd   2019

  • Effect of phospholipids on human flippase ATP8A1 reaction mechanism.

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, SHIN Hye-Won, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   45th   2019

  • Analysis of Tyr<sup>122</sup>-hydorophobic cluster mutants of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase with equations of classical physical chemistry-Exploring the method to quantify the contribution of hydrophobic-interactions in enzymatic reaction-

    山崎和生, 山崎誠一郎, 市村佑人, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   45th   2019

  • Ca<sup>2+</sup>-ATPase変異による進行性聴力障害について

    美野輪治, 美野輪治, 美野輪治, 神谷和作, 大保貴嗣, 山崎和生, 鈴木裕, 吉木淳, 城石俊彦, 野田哲生, 野田哲生, 岡崎康司, 池田勝久

    耳鼻咽喉科ニューロサイエンス   33   2019

  • ヒトフリッパーゼATP8A1の反応機構解析

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, SHIN Hye-Won, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   44th   2018

  • フリッパーゼATP8A1の反応と安定性に対する界面活性剤および脂質の影響

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, 申惠媛, DANKO Stefania, 鈴木裕

    日本生化学会大会(Web)   91st   2018

  • 筋小胞体Ca<sup>2+</sup>-ATPaseの脱共役モードについて

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   44th   2018

  • 筋小胞体カルシウムポンプのCa<sup>2+</sup>結合に及ぼす界面活性剤の効果

    大保貴嗣, DANKO Stefania, 山崎和生, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   44th   2018

  • 筋小胞体カルシウムポンプの非リン酸化中間体におけるCa2+結合に及ぼす界面活性剤の影響

    大保貴嗣, 山崎和生, DANKO Stefania, 安田哲, 鈴木裕

    日本生化学会大会(Web)   91st   2018

  • ナノディスク中の筋小胞体Ca<sup>2+</sup>-ATPaseに標識された単一蛍光分子の角度と明滅による中間体構造の評価

    竹村勁哉, 小島知樹, 山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕, 政池知子

    日本生体エネルギー研究会討論会講演要旨集   44th   2018

  • デフォーカスイメージング法の確度評価とSERCA1aの構造変化検出に向けた試み

    加藤孝信, 藤村章子, 山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕, 西坂崇之

    日本生体エネルギー研究会討論会講演要旨集   44th   2018

  • 筋小胞体Ca<sup>2+</sup>-ATPaseの脱共役モードについて

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生化学会大会(Web)   91st   2018

  • フリッパーゼの生化学的反応機構の解析

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, 申惠媛, DANKO Stefania, 鈴木裕

    日本生化学会大会(Web)   90th   2017

  • 筋小胞体カルシウムポンプ活性に対する脂質ヘッドグループ及び膜表面電荷の影響の解析

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   43rd   2017

  • 筋小胞体カルシウムポンプの活性に対する界面活性剤の影響

    大保貴嗣, 山崎和生, DANKO Stefania, 安田哲, 鈴木裕

    日本生化学会大会(Web)   90th   2017

  • 筋小胞体カルシウムポンプの反応に及ぼす界面活性剤の効果

    大保貴嗣, 山崎和生, DANKO Stefania, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   43rd   2017

  • フリッパーゼの反応機構解析

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, SHIN Hye-Won, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   43rd   2017

  • ナノディスクに組み込んだ筋小胞体Ca<sup>2+</sup>-ATPaseの活性に対する膜表面電荷の影響

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生化学会大会(Web)   90th   2017

  • 脂質ヘッドグループが筋小胞体カルシウムポンプ活性に及ぼす影響-ナノディスク組み込み標品を用いて-

    山崎和生, 大保貴嗣, STEFANIA Danko, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   42nd   2016

  • ナノディスクに組み込んだ標品を用いた筋小胞体Ca<sup>2+</sup>-ATPaseのEP転換ステップに及ぼす脂質組成及びA23187の影響の解析

    山崎和生, 大保貴嗣, STEFANIA Danko, 鈴木裕

    日本生化学会大会(Web)   89th   2016

  • Caポンプのエネルギー共役;M2-helix膜貫通部分(M2m)と細胞質部分(M2c)をつなぐGly105の柔軟性の機能

    大保貴嗣, 山崎和生, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   42nd   2016

  • 筋小胞体Ca2+ポンプM2ヘリックスの膜貫通部分と細胞質部分の連結領域:エネルギー共役における役割

    大保貴嗣, 山崎和生, ダンコ ステファニア, 鈴木裕

    日本生化学会大会(Web)   89th   2016

  • Caポンプの素過程構造-機能連関:部位特異的変異,速度論,および中間体・遷移状態捕捉による解析

    鈴木裕, 大保貴嗣, 山崎和生, STEFANIA Danko

    日本生化学会大会(Web)   89th   2016

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのEP転換ステップにおけるTyr<sup>122</sup>-Hydorophobic clusterの集合過程とエネルギー共役

    山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕

    日本生化学会大会(Web)   88th   2015

  • 筋小胞体Ca<sup>2+</sup>ポンプのエネルギー共役におけるM2ヘリックス膜貫通部分(M2<sub>m</sub>)と細胞質部分(M2<sub>cyt</sub>)の連結領域の役割

    大保貴嗣, 山崎和生, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   41st   2015

  • 筋小胞体Ca<sup>2+</sup>ポンプのエネルギー共役におけるM2ヘリックス膜貫通部分(M2<sub>m</sub>)と細胞質部分(M2<sub>cyt</sub>)の連結領域の役割

    大保貴嗣, 山崎和生, DANKO Stefania, 鈴木裕

    日本生化学会大会(Web)   88th   2015

  • ナノディスクへ組み込んだ筋小胞体カルシウムポンプを用いたタンパク質-脂質相互作用の解析

    山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   41st   2015

  • ナノディスクに組み込んだ筋小胞体Ca<sup>2+</sup>-ATPaseの活性に対する脂質ヘッドグループの影響の解析

    山崎和生, STEFANIA Danko, 大保貴嗣, 鈴木裕

    日本生化学会大会(Web)   87th   2014

  • 筋小胞体Ca<sup>2+</sup>ポンプのM2ヘリックス:膜貫通部分と細胞質部分の連結領域の役割

    大保貴嗣, 山崎和生, STEFANIA Danko, 鈴木裕

    日本生化学会大会(Web)   87th   2014

  • 筋小胞体Ca<sup>2+</sup>-ATPaseの第2膜貫通ヘリックス(M2)とロングレンジの共役

    大保貴嗣, 山崎和生, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   40th   2014

  • 筋小胞体カルシウムポンプのEP転換とカルシウム放出の共役機構におけるTyr<sup>122</sup>-hydrophobic clusterの役割

    山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   40th   2014

  • 1分子蛍光観察によるCa<sup>2+</sup>-ATPaseの数と立体構造変化検出の試み

    小島知樹, 戸田啓太, 山崎和生, DANKO Stefania, 小山洋幸, 横田龍一, 中山莉奈子, 中山莉奈子, 大保貴嗣, 鈴木裕, 政池知子, 政池知子

    日本生体エネルギー研究会討論会講演要旨集   40th   2014

  • 筋小胞体カルシウムポンプのナノディスクへの組み込みとその性質

    山崎和生, DANKO Stefania, 大保貴嗣, 鈴木裕

    日本生化学会大会(Web)   86th   2013

  • 筋小胞体Ca<sup>2+</sup>ポンプのヘリックスM2膜貫通部分と細胞質部分,およびその連結領域の役割

    大保貴嗣, 山崎和生, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   39th   2013

  • 筋小胞体カルシウムポンプのナノディスクへの組み込みとその性質

    山崎和生, STEFANIA Danko, 大保貴嗣, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   39th   2013

  • 筋小胞体Ca<sup>2+</sup>ポンプのヘリックスM2膜貫通部分(M2<sub>m</sub>)と細胞質部分(M2<sub>cyt</sub>)およびその連結領域の役割

    大保貴嗣, 山崎和生, DANKO Stefania, 鈴木裕

    日本生化学会大会(Web)   86th   2013

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのE1P-E2P転換とCa<sup>2+</sup>放出の共役機構

    山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕

    日本生化学会大会(Web)   85th   2012

  • 筋小胞体Ca<sup>2+</sup>ポンプにおけるエネルギー共役;膜貫通ヘリックスM2およびその細胞質領域アクチュエータードメインとの連結部分の役割

    大保貴嗣, DANKO Stefania, 山崎和生, 鈴木裕

    日本生化学会大会(Web)   85th   2012

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのE1P-E2P転換とCa<sup>2+</sup>放出の共役機構

    山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   38th   2012

  • 筋小胞体Ca<sup>2+</sup>ポンプの輸送反応における膜貫通ヘリックスM2の役割

    大保貴嗣, DANKO Stefania, 山崎和生, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   38th   2012

  • 筋小胞体Ca<sup>2+</sup>ポンプにおけるエネルギー共役;細胞質領域アクチュエータードメインと膜貫通ヘリックスとの連結部分の役割

    大保貴嗣, DANKO Stefania, 山崎和生, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   37th   2011

  • 筋小胞体Ca<sup>2+</sup>-ATPaseリン酸化中間体転換ステップにおける静電相互作用の効果とその定量化の試み

    山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   37th   2011

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのリン酸化中間体転換過程における静電的相互作用の役割

    山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕

    生化学   2010

  • 筋小胞体Ca<sup>2+</sup>-ATPaseの反応サイクルにおける静電的相互作用の役割とイオン強度依存性との関連

    山崎和生, 大保貴嗣, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   36th   2010

  • 筋小胞体Ca<sup>2+</sup>-ATPaseによるCa<sup>2+</sup>輸送;細胞質領域アクチュエータードメインと膜貫通ヘリックスを結ぶループの役割

    大保貴嗣, DANKO Stefania, 山崎和生, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   36th   2010

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのリン酸化中間体の異性化と加水分解に影響を及ぼす構造因子

    大保貴嗣, DANKO Stefania, 山崎和生, 鈴木裕

    生化学   2010

  • Ca<sup>2+</sup>ポンプの分子作動機構:部位特異的変異およびリン酸化中間体アナログの開発と構造解析による理解

    鈴木裕, 山崎和生, 大保貴嗣, DANKO Stefania

    日本薬学会年会要旨集   129th ( 1 )   2009

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのElP-E2P転換とCa<sup>2+</sup>輸送の共役におけるK<sup>+</sup>の役割

    山崎和生, 大保貴嗣, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   35th   2009

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのE1P-E2P転換とCa<sup>2+</sup>輸送の共役におけるK<sup>+</sup>の役割

    山崎和生, 大保貴嗣, 鈴木裕

    生化学   2009

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのリン酸化中間体転換とCa<sup>2+</sup>輸送;細胞質領域アクチュエータードメインと膜貫通ヘリックスを結ぶループの役割

    大保貴嗣, DANKO Stefania, 山崎和生, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   35th   2009

  • ATP2C1 is specifically localized in the basal layer of normal epidermis and its depletion triggers differentiation of keratinocytes via manganese starvation in the Golgi apparatus

    M. Yoshida, K. Yamasaki, T. Daiho, M. S. Matsui, H. Iizuka, H. Suzuki, M. Ichihashi

    JOURNAL OF INVESTIGATIVE DERMATOLOGY   128   S90 - S90   2008.4

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  • 筋小胞体Ca<sup>2+</sup>-ATPaseのE2PCa<sub>2</sub>におけるTyr122-Hydrophobic clusterの果たす役割について

    山崎和生, WANG Guoli, 大保貴嗣, 鈴木裕

    生化学   2008

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのCa<sup>2+</sup>結合型-リン酸化中間体アナログの安定性に及ぼすヌクレオチドの効果

    大保貴嗣, 山崎和生, DANKO Stefania, 鈴木裕

    生化学   2008

  • 筋小胞体カルシウムポンプ:リン酸化中間体の転換にともなうCa<sup>2+</sup>の脱閉塞機構

    大保貴嗣, 山崎和生, 王国麗, STEFANIA Danko, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   33rd   2007

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのCa<sup>2+</sup>放出過程におけるTyr<sup>122</sup>-Hydrophobic clusterとK<sup>+</sup>との関連

    山崎和生, WANG Guoli, 大保貴嗣, 鈴木裕

    生化学   2007

  • 筋小胞体Ca<sup>2+</sup>-ATPaseの変異体によるCa<sup>2+</sup>を閉塞したADP非感受性リン酸化中間体のアナログの形成

    大保貴嗣, DANKO Stefania, 山崎和生, 鈴木裕

    生化学   2007

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのP-Nヒンジ領域に存在する荷電側鎖の役割

    山崎和生, 大保貴嗣, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   33rd   2007

  • Changes in interactions between phosphorylation- and actuator-domains of sarco(endo) lasmic reticulum Ca22+-ATPase during processing of phosphoenzyme intermediate.

    K Yamasaki, T Daiho, W Guoli, S Danko, H Suzuki

    JOURNAL OF GENERAL PHYSIOLOGY   126 ( 1 )   32A - 32A   2005.7

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  • Multiple and Distinct Effects of Mutations of Tyr122, Glu123, Arg324, and Arg334 Involved in Interactions between the Top Part of Second and Fourth Transmembrane Helices in Sarcoplasmic Reticulum Ca2+-ATPase: CHANGES IN CYTOPLASMIC DOMAIN ORGANIZATION ・・・ Reviewed

    Journal of Biological Chemistry   279 ( (3) )   2202 - 2210   2004.4

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    Multiple and Distinct Effects of Mutations of Tyr122, Glu123, Arg324, and Arg334 Involved in Interactions between the Top Part of Second and Fourth Transmembrane Helices in Sarcoplasmic Reticulum Ca2+-ATPase: CHANGES IN CYTOPLASMIC DOMAIN ORGANIZATION DURING ISOMETRIC TRANSITION OF PHOSPHOENZYME INTERMEDIATE AND SUBSEQUENT Ca2+ RELEASE

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  • 当科で報告したダリエー病の遺伝子変異を導入したCa<sup>2+</sup>ポンプ蛋白質の発現量及び機能解析

    佐藤克彦, 宮内勇貴, 中村哲史, 高橋英俊, 山本明美, 橋本喜夫, 山崎和生, 大保貴嗣, 鈴木裕

    日本皮膚科学会雑誌   114 ( 11 )   2004

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのAドメイン 膜貫通ヘリックス連結領域の部位特異的変異

    大保貴嗣, 山崎和生, 王国麗, STEFANIA D, 鈴木裕

    生化学   74 ( 8 )   2002

  • 筋小胞体Ca<sup>2+</sup>-ATPaseの細胞質ドメインに存在するイオンペアの役割

    山崎和生, 大保貴嗣, 鈴木裕

    生化学   74 ( 8 )   2002

  • ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+-ATPase has a compact conformation resistant to proteinase K, V8 protease and trypsin

    S Danko, T Daiho, K Yamasaki, M Kamidochi, H Suzuki, C Toyoshima

    FEBS LETTERS   489 ( 2-3 )   277 - 282   2001.2

  • Proteinase K,V8,Trypsinによる限定分解によって明らかになった筋小胞体Ca<sup>2+</sup>-ATPaseの触媒過程におけるドメイン集合状態変化

    DANKO S, 山崎和生, 大保貴嗣, 鈴木裕, 豊島近

    生化学   73 ( 8 )   2001

  • Ca<sup>2+</sup>非存在下での可溶化筋小胞体Ca<sup>2+</sup>-ATPaseのATP,ADP,及びMg<sup>2+</sup>による安定化とイオン強度の影響

    山崎和生, 大保貴嗣, 鈴木裕

    生化学   73 ( 8 )   2001

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのVal-200への変異導入はリン酸化中間体の迅速な異性化と加水分解を阻害する

    加藤早苗, 上堂地美佳, 大保貴嗣, 山崎和生, 鈴木裕

    生化学   73 ( 8 )   2001

  • 筋小胞体Ca<sup>2+</sup>-ATPase(SERCA1a)のCys残基の部位特異的変異

    大保貴嗣, 王国麗, 山崎和生, 加藤早苗, 鈴木裕

    生化学   73 ( 8 )   2001

  • Mutations of arginine-198 in sarcoplasmic reticulum Ca2+-ATPase cause inhibition of hydrolysis of the phosphoenzyme intermediate formed from inorganic phosphate

    K Yamasaki, T Daiho, H Suzuki, T Saino, T Kanazawa

    NA/K-ATPASE AND RELATED ATPASES   1207   297 - 300   2000

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  • 筋小胞体Ca<sup>2+</sup>-ATPaseのマグネシウム・フッ素複合体はCa<sup>2+</sup>非存在下でのC<sub>12</sub>E<sub>8</sub>による可溶化に対して安定である

    山崎和生, 大保貴嗣, 上堂地美佳, 鈴木裕

    生化学   72 ( 8 )   2000

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのC876-C888ジスルフィド結合はATP加水分解とCa<sup>2+</sup>輸送の共役に重要である

    大保貴嗣, 山崎和生, 上堂地美佳, 鈴木裕

    生化学   72 ( 8 )   2000

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのVal-200への部位特異的変異導入はCa<sup>2+</sup>-ATPase活性を抑制する

    上堂地美佳, 大保貴嗣, 山崎和生, 鈴木裕

    生化学   72 ( 8 )   2000

  • Deletions or specific substitutions of a few residues in the NH2-terminal region (Ala(3) to Thr(9)) of sarcoplasmic reticulum Ca2+-ATPase cause inactivation and rapid degradation of the enzyme expressed in COS-1 cells

    T Daiho, K Yamasaki, H Suzuki, T Saino, T Kanazawa

    JOURNAL OF BIOLOGICAL CHEMISTRY   274 ( 34 )   23910 - 23915   1999.8

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  • Mutations of Arg(198) in sarcoplasmic reticulum Ca2+-ATPase cause inhibition of hydrolysis of the phosphoenzyme intermediate formed from inorganic phosphate

    T Daiho, H Suzuki, K Yamasaki, T Saino, T Kanazawa

    FEBS LETTERS   444 ( 1 )   54 - 58   1999.2

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    Arg(198) of sarcoplasmic reticulum Ca2+-ATPase was substituted with lysine, glutamine, glutamic acid, alanine, and isoleucine by site-directed mutagenesis. Kinetic analysis was performed with microsomal membranes isolated from COS-I cells which were transfected,vith the mutated cDNAs. The rate of dephosphorylation of the ADP-insensitive phosphoenzyme was determined by first phosphorylating the Ca2+-ATPase with P-32(i) and then diluting the sample with non-radioactive P-i. This rate mas reduced substantially in the mutant R198Q, more strongly in the mutants R198A and R198I, and most strongly in the mutant R198E, but to a much lesser extent in R198K. The reduction in the rate of dephosphorylation was consistent with the observed decrease in the turnover rate of the Ca2+-ATPase accompanied by the steady-state accumulation of the ADP-insensitive phosphoenzyme formed from ATP. These results indicate that the positive charge and high hydrophilicity. of Arg(198) are critical for rapid hydrolysis of the ADP-insensitive phosphoenzyme. (C) 1999 Federation of European Biochemical Societies.

    DOI: 10.1016/S0014-5793(99)00027-7

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  • Mutations of Arg<sup>198</sup> in sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase Cause Inactivation and Rapid Degradation of the Enzyme Expressed in COS-1 Cells(jointly worked)

    FEBS Letter   444   54 - 58   1999

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのN末端領域(Ala3-Thr9)における2-3残基の削除または特異的置換は失活とCOS-1細胞内での速い分解を引き起こす

    大保貴嗣, 山崎和生, 鈴木裕, 斎野朝幸, 金沢徹

    生体エネルギー研究会討論会講演要旨集   25th   1999

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのN末端ドメインの数残基によるnative conformationの安定化,およびArg198への変異導入によるリン酸化中間体の加水分解の阻害

    大保貴嗣, 山崎和生, 鈴木裕, 斎野朝幸, 金沢徹

    生体エネルギー研究会討論会講演要旨集   24th   1998

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのHis-5のジエチルピロカーボネートによる修飾はリン酸化中間体の加水分解を阻害する

    大保貴嗣, 山崎和生, 斎野朝幸, 金沢徹

    生体エネルギー研究会討論会講演要旨集   23rd   1997

  • Conformation change in catalysis process of sarcoplasmic reticulum Ca2+-ATPase.

    金沢徹, 鈴木裕, 大保貴嗣, 山崎和生

    日本分子生物学会年会プログラム・講演要旨集   19th   33 - 33   1996.8

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  • Identification of arginyl residues located at the ATP binding site of sarcoplasmic reticulum Ca2+-ATPase: Modification with 1,2-cyclohexanedione

    Kimura, K., Suzuki, H., Daiho, T., Yamasaki, K., Kanazawa, T.

    Journal of Biological Chemistry   271 ( 46 )   28933 - 28941   1996

  • Functional analysis and identification of arginine residue which exists for ATP binding site of sarcoplasmic reticulum Ca2+-ATPase.

    木村圭介, 鈴木裕, 大保貴嗣, 山崎和生, 金沢徹

    日本分子生物学会年会プログラム・講演要旨集   19th   1996

  • Fluorometric study on conformational changes in the catalytic cycle of sarcoplasmic reticulum Ca2+-ATPase

    Tohru Kanazawa, Hiroshi Suzuki, Takashi Daiho, Kazuo Yamasaki

    Bioscience Reports   15 ( 5 )   317 - 326   1995.10

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    Language:English   Publishing type:Book review, literature introduction, etc.   Publisher:Kluwer Academic Publishers-Plenum Publishers  

    DOI: 10.1007/BF01788364

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  • IRRADIATION WITH ULTRAVIOLET-LIGHT IN THE PRESENCE OF VANADATE INCREASES CA2+ PERMEABILITY OF THE SARCOPLASMIC-RETICULUM MEMBRANE VIA CA2+-ATPASE

    T HIROSE, K YAMASAKI, T YAMAMOTO

    JOURNAL OF BIOCHEMISTRY   117 ( 2 )   324 - 330   1995.2

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  • 3'-O-(5-Fluoro-2, 4-dinitro phenyl)ATP Exclusively Labels Lys-492 at the Active Site of the Sarcoplasmic Reticulum Ca<sup>2+</sup>-ATPase (jointly worked)

    Journal of Biological Chemistry   269 ( 6 )   4129   1994

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  • INHIBITION OF PHOSPHOENZYME FORMATION FROM PHOSPHATE AND SARCOPLASMIC-RETICULUM CA2+-ATPASE BY VANADATE BINDING TO HIGH-AFFINITY OR LOW-AFFINITY SITE ON THE ENZYME

    K YAMASAKI, T YAMAMOTO

    JOURNAL OF BIOCHEMISTRY   112 ( 5 )   658 - 664   1992.11

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  • EXISTENCE OF HIGH-AFFINITY AND LOW-AFFINITY VANADATE-BINDING SITES ON CA2+-ATPASE OF THE SARCOPLASMIC-RETICULUM

    K YAMASAKI, T YAMAMOTO

    JOURNAL OF BIOCHEMISTRY   110 ( 6 )   915 - 921   1991.12

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  • DETERMINATION OF THE PRIMARY STRUCTURE OF INTERMOLECULAR CROSS-LINKING SITES ON THE CA2+-ATPASE OF SARCOPLASMIC-RETICULUM USING C-14-LABELED N,N'-(1,4-PHENYLENE)BISMALEIMIDE OR N-ETHYLMALEIMIDE

    K YAMASAKI, N SANO, M OHE, T YAMAMOTO

    JOURNAL OF BIOCHEMISTRY   108 ( 6 )   918 - 925   1990.12

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  • Effects of Adenyl-5'-Imidodiphosphate and Vanadate Ion on the Intermolecular Cross-Linking of Ca<sup>2+</sup>-ATPase in the Sarcoplasmic Reticulum Membrane with N, N'-(1,4-phenylene)bismaleimide (jointly worked)

    Yamasaki Kazuo, Yamamoto Taibo

    Journal of Biochemistry   106 ( 6 )   1114 - 1120   1989

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    The functional significance of the molecular interaction of Ca<SUP>2+</SUP>-ATPase in the sarcoplasmic reticulum (SR) membrane was examined using intermolecular cross-linking of Ca<SUP>2+</SUP>-ATPase with <I>N, N</I>'-(1, 4-phenylene) bismaleimide (PBM). When SR vesicles were allowed to react with 1mM PBM at pH 7 and 23°C for various intervals and subjected to SDS-PAGE, the amount of the major band of monomeric ATPase decreased with a half life of about 20min. Higher orders of oligomers were concurrently formed without accumulation of any particular species of oligomer. When SR vesicles were allowed to react with 1 mM PBM in the presence of 1 mM adenyl-5'-imidodiphosphate (AMP-PNP), the rate of oligomerization was markedly reduced and the amount of dimeric Ca<SUP>2+</SUP>-ATPase increased with time. After 1h, more than 40% of the Ca<SUP>2+</SUP>-ATPase had accumulated in the dimeric form. When 1 mol of fluorescein isothiocyanate (FITC) was bound per mol of ATPase, the effects of AMP-PNP on the cross-linking with PBM were completely abolished. When SR vesicles were treated with PBM in the presence of 0.1 mM vanadate in Ca<SUP>2+</SUP> free medium, the oligomerization of the Ca<SUP>2+</SUP>-ATPase by PBM was strongly inhibited. The vanadate effect on the cross-link formation was completely removed by the presence of Ca<SUP>2+</SUP> and AMP-PNP in the reaction medium. When SR vesicles were pretreated with PBM in the presence of AMP-PNP and digested with trypsin for a short time, the dimeric ATPase was degraded to a peptide with an apparent molecular mass of about 170kDa. Further digestion resulted in degradation to a 130 kDa peptide. As they were phosphorylated by [γ-<SUP>32</SUP>P] ATP in the presence of Ca<SUP>2+</SUP>, these peptides contained the subfragment A. In addition, the SDS-PAGE pattern of the dimeric ATPase after the prolonged digestion lacked subfragment A<SUB>1</SUB>, suggesting that ATPase molecules were cross-linked by PBM through an SH group in the A<SUB>1</SUB> region of this enzyme.

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Presentations

  • Uncoupling mechanism of the sarcoplasmic reticulum calcium pump revealed by temperature dependence

    Kazuo Yamasaki, Satoshi Yasuda, Jun-ichi Kawabe

    2024.12 

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    Event date: 2024.12

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  • Uncoupling mechanism of the sarcoplasmic reticulum calcium pump revealed by temperature dependence

    Kazuo Yamasaki, Satoshi Yasuda, Jun-ichi Kawabe

    2024.11 

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    Event date: 2024.11

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  • 筋小胞体Caポンプのリン酸化中間体の反応とA-ドメインのはたらきについて

    〇大保 貴嗣, 山崎 和生, 川辺 淳一

    日本生体エネルギー研究会 第49回討論会 

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    Event date: 2023.12

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  • 筋小胞体Ca2+-ATPaseのリン酸化中間体形成過程に対する脂質の違いによる影響

    〇山崎 和生, 安田 哲, 大保 貴嗣, 川辺 淳一

    日本生体エネルギー研究会 第49回討論会 

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    Event date: 2023.12

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  • 筋小胞体CaポンプのヘリックスM2/M6/M4のCa輸送における役割

    〇大保 貴嗣, 山崎 和生, 川辺 淳一

    第96回日本生化学会大会 

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    Event date: 2023.10 - 2023.11

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  • 筋小胞体Ca2+-ATPaseのリン酸化中間体形成過程に対する脂質の違いによる影響

    〇山崎 和生, 安田 哲, 大保 貴嗣, 川辺 淳一

    第96回日本生化学会大会 

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    Event date: 2023.10 - 2023.11

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  • 筋?胞体CaポンプのヘリックスM2/M6相互作?のエネルギー共役における役割

    大保貴嗣、山崎和生、安田哲、川辺淳一

    日本生体エネルギー研究会第48回討論会 

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    Event date: 2022.12

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  • 混合脂質環境における筋?胞体Ca2+-ATPaseの特性の変化について

    山崎 和生、安田 哲、大保 貴嗣、川辺 淳一

    日本生体エネルギー研究会第48回討論会 

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    Event date: 2022.12

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  • 筋⼩胞体CaポンプのヘリックスM2/M6相互作⽤のエネルギー共役における役割

    大保貴嗣, 山崎和生, 安田哲, 川辺淳一

    日本生体エネルギー研究会第48回討論会 

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    Event date: 2022.12

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    Venue:京都  

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  • 混合脂質環境における筋⼩胞体Ca2+-ATPaseの特性の変化について

    山崎 和生, 安田 哲, 大保 貴嗣, 川辺 淳一

    日本生体エネルギー研究会第48回討論会 

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    Event date: 2022.12

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    Venue:京都  

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  • 混合脂質環境における筋小胞体Ca2+-ATPaseの特性の変化について

    山崎 和生、安田 哲、大保 貴嗣、川辺 淳一

    第95回 日本生化学会大会 

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    Event date: 2022.11

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  • 筋小胞体CaポンプのヘリックスM2/M6のCa輸送における役割

    大保貴嗣、山崎和生、安田哲、川辺淳一

    第95回 日本生化学会大会 

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    Event date: 2022.11

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  • Role of M2 and M6 Helices of Sarcoplasmic Reticulum Ca Pump in Ca Transport. International conference

    Takashi Daiho, Kazuo Yamasaki

    16th International Conference on Na,K-ATPase and Related Transport ATPases: P-Type ATPases in Health and Disease 

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    Event date: 2022.9

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  • Roles of Intra-Protein and Protein-Lipids Interactions Including Arg324 of Sarcoplasmic Reticulum Ca2+-ATPase. International conference

    Kazuo Yamasaki, Takashi Daiho, Satoshi Yasuda, Stefania Danko, Jun-ichi Kawabe and Hiroshi Suzuki

    16th International Conference on Na,K-ATPase and Related Transport ATPases: P-Type ATPases in Health and Disease 

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    Event date: 2022.9

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  • 筋小胞体カルシウムポンプの多機能性残基 Arg324 と脂質との相互作用の多様性について

    ○山崎和生、 大保貴嗣、 Danko Stefania、安田 哲、川辺 淳一、 鈴木裕

    日本生体エネルギー研究会第47回討論会 

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    Event date: 2021.12

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  • Defocus imaging 法による SERCA1aの Aドメインの角度変化検出

    加藤孝信 、大保貴嗣 、山崎和生、 Stefania Danko、藤村章子、鈴木 裕

    日本生体エネルギー研究会第47回討論会 

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    Event date: 2021.12

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  • 筋小胞体 Ca2+ポンプの M2 ヘリックス:膜貫通部分のエネルギー共役における役割

    ○大保貴嗣、 山崎和生、 川辺 淳一

    日本生体エネルギー研究会第47回討論会 

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    Event date: 2021.12

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  • 筋小胞体Ca2+ポンプのエネルギー共役:細胞質-膜貫通領域間における構造変化の伝達

    ○大保貴嗣、 山崎和生、 川辺 淳一

    第94回 日本生化学会大会 

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    Event date: 2021.11

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  • 筋小胞体カルシウムポンプの多機能側鎖Arg324と多種のリン脂質のアンサンブル

    ○山崎和生、 大保貴嗣、 Danko Stefania、安田 哲、川辺 淳一、 鈴木裕

    第94回 日本生化学会大会 

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    Event date: 2021.11

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  • 筋小胞体カルシウムポンプにおける静電相互作用の役割 ータンパク内、タンパク-脂質間の静電的リンクについてー Invited

    山崎 和生

    大阪大学蛋白質研究所セミナー「生体膜上の生物化学 -解析法の進展から細胞内オルガネラのバイオロジーまで-」 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 筋小胞体カルシウムポンプの結晶構造中に見られたArg324とリン脂質間のリンクが果たす役割について

    ○山崎 和生、大保 貴嗣、安田 哲、Stefania Danko、川辺 淳一、鈴木 裕

    第46回日本生体エネルギー研究会 

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    Language:Japanese   Presentation type:Oral presentation (general)  

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  • ヒトフリッパーゼATP8A1の反応機構

    ○安田 哲、山崎 和生、大保 貴嗣、高津 宏之、申 惠媛、Stefania Danko、川辺 淳一、 鈴木 裕

    第46回日本生体エネルギー研究会 

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    Event date: 2020.12

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  • 筋小胞体Ca2+ポンプの膜貫通ヘリックスM2 のエネルギー共役におけ る役割

    ○大保貴嗣、山崎和生、安田哲、川辺淳一

    第46回日本生体エネルギー研究会 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 筋小胞体カルシウムポンプの結晶構造中に見られたArg324とリン脂質間のリンクが果たす役割について

    ○山崎 和生、大保 貴嗣、安田 哲、Stefania Danko、川辺 淳一、鈴木 裕

    第93回日本生化学会大会 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • ヒトフリッパーゼATP8A1とCaポンプの反応機構の比較

    安田 哲, 山崎 和生, 大保 貴嗣, 高津 宏之, 申 惠媛, Danko Stefania, 川辺 淳一, 鈴木 裕

    第93回日本生化学会大会 

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    Event date: 2020.9

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  • 筋小胞体Ca2+ポンプのM2ヘリックス:膜貫通部分のエネルギー共役における役割

    ○大保 貴嗣, 山崎 和生, 安田 哲, 川辺 淳一

    第93回日本生化学会大会 

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    Event date: 2020.9

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  • 筋小胞体カルシウムポンプTyr122-疎水性クラスター変異体の古典的物理化学公式を用いた解析-酵素反応における疎水性結合の寄与の定量化の試み―

    〇山崎和生、山崎誠一郎、市村佑人、大保貴嗣、Stefania Danko、安田哲、鈴木裕

    第45回日本生体エネルギー研究会 

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    Event date: 2019.12

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  • 筋小胞体Ca2+ポンプの第2膜貫通ヘリックス:膜貫通部分のエネルギー共役における役割

    ○大保貴嗣、山崎和生、Stefania Danko、安田哲

    第45回日本生体エネルギー研究会 

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    Event date: 2019.12

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  • ヒトフリッパーゼATP8A1の各反応素過程におけるリン脂質の影響

    ○安田哲、山崎 和生、大保 貴嗣、高津 宏之、申 惠媛、Stefania Danko、鈴木 裕

    第45回日本生体エネルギー研究会 

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  • 筋小胞体カルシウムポンプのTyr122クラスター変異体で観測された異常に遅いEP分解

    〇山崎和生、市村佑人、山崎誠一郎、大保貴嗣、Stefania Danko、安田哲、鈴木裕

    第92回日本生化学会 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • ヒトフリッパーゼATP8A1の反応機構における脂質の影響

    ○安田哲、山崎 和生、大保 貴嗣、高津 宏之、申 惠媛、Stefania Danko、鈴木 裕

    第92回日本生化学会 

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    Event date: 2019.9

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  • 筋小胞体Ca2+ポンプの第2膜貫通ヘリックス:膜貫通部分のエネルギー共役における役割

    ○大保貴嗣、山崎和生、Stefania Danko、安田哲

    第92回日本生化学会 

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    Event date: 2019.9

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  • ヒトフリッパーゼATP8A1の反応機構

    安田 哲、山崎 和生、大保 貴嗣、高津 宏之、申 惠媛、Stefania Danko、鈴木 裕

    日本生体エネルギー研究会 第44回討論会 

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    Event date: 2018.12

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  • ナノディスク中の筋小胞体Ca2+-ATPaseに標識された単一分子の角度と明滅による中間体構造の評価

    竹村 勁哉、小島 知樹、山崎 和生、大保 貴嗣、Stefania Danko、鈴木 裕、政池 知子

    日本生体エネルギー研究会 第44回討論会 

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    Event date: 2018.12

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  • デフォーカスイメージング法の確度評価とSERCA1aの構造変化検出に向けた試み

    加藤 孝信、藤村 章子、山崎 和生、大保 貴嗣、Danko Stefania、鈴木 裕、 西坂 崇之

    日本生体エネルギー研究会 第44回討論会 

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    Event date: 2018.12

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  • 筋小胞体カルシウムポンプのCa2+結合に及ぼす界面活性剤の効果

    大保 貴嗣、山崎 和生、Danko Stefania、安田 哲、鈴木 裕

    日本生体エネルギー研究会 第44回討論会 

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    Event date: 2018.12

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  • 筋小胞体Ca2+-ATPaseの脱共役モードについて

    山崎和生、 大保貴嗣、 Danko Stefania、安田 哲、 鈴木裕

    日本生体エネルギー研究会 第44回討論会 

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    Event date: 2018.12

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  • 筋小胞体Ca2+-ATPaseの脱共役モードについて

    山崎 和生, 大保 貴嗣, Stefania Danko, 安田 哲, 鈴木 裕

    第91回日本生化学会大会 

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    Event date: 2018.9

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  • 筋小胞体カルシウムポンプの非リン酸化中間体におけるCa2+結合に及ぼす界面活性剤の影響

    大保 貴嗣, 山崎 和生, Stefania Danko, 安田 哲, 鈴木 裕

    第91回日本生化学会大会 

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    Event date: 2018.9

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  • フリッパーゼATP8A1の反応と安定性に対する界面活性剤および脂質の影響

    安田 哲、 山崎 和生、大保 貴嗣、高津 宏之、申 惠媛、 Stefania Danko、鈴木 裕

    第91回日本生化学会大会 

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    Event date: 2018.9

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  • 筋小胞体カルシウムポンプ活性に対する脂質ヘッドグループ及び膜表面電荷の影響の解析

    山崎和生、 大保貴嗣、 Danko Stefania、安田 哲、 鈴木裕

    日本生体エネルギー研究会 第43回討論会 

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    Event date: 2017.12

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  • 筋小胞体カルシウムポンプの反応に及ぼす界面活性剤の効果

    大保 貴嗣、山崎 和生、Danko Stefania、安田 哲、鈴木 裕

    日本生体エネルギー研究会 第43回討論会 

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    Event date: 2017.12

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  • フリッパーゼの反応機構解析

    安田 哲、山崎和生、 大保貴嗣、高津 宏之、申 惠媛、 Danko Stefania、 鈴木裕

    日本生体エネルギー研究会 第43回討論会 

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    Event date: 2017.12

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  • ナノディスクに組み込んだ筋小胞体Ca2+-ATPaseの活性に対する膜表面電荷の影響

    山崎和生、 大保貴嗣、 Danko Stefania、安田 哲、 鈴木裕

    生命科学系学会合同年次大会(ConBio2017) 

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  • フリッパーゼの生化学的反応機構の解析

    安田 哲、山崎和生、 大保貴嗣、高津 宏之、申 惠媛、 Danko Stefania、 鈴木裕

    生命科学系学会合同年次大会(ConBio2017) 

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  • 筋小胞体カルシウムポンプの活性に対する界面活性剤の影響

    大保 貴嗣、山崎 和生、Danko Stefania、安田 哲、鈴木 裕

    生命科学系学会合同年次大会(ConBio2017) 

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  • Effects of phospholipid's head groups on the properties of sarcoplasmic reticulum Ca2+-ATPase embedded in nanodisc International conference

    K. Yamasaki, T. Daiho, S. Danko, S. Yasuda and H. Suzuki

    The 15th Inthernational Conference on Na,K-ATPase and Related Transport ATPases 

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    Event date: 2017.9

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  • Membrane Perturbation of ADP-insensitive Phosphoenzyme of Ca2+-ATPase Modifies Gathering of Transmembrane Helix M2 with Cytoplasmic Domains and Luminal Gating International conference

    S. Danko, K. Yamasaki, T. Daiho, S. Yasuda and H. Suzuki

    The 15th Inthernational Conference on Na,K-ATPase and Related Transport ATPases 

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    Event date: 2017.9

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  • Glycine 105 as Pivot for a Critical Knee-like Joint between Cytoplasmic and Transmembrane Segments of the Second Transmembrane Helix in Ca2+-ATPase International conference

    Takashi Daiho, Kazuo Yamasaki, Stefania Danko and Hiroshi Suzuki

    The 15th Inthernational Conference on Na,K-ATPase and Related Transport ATPases 

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    Event date: 2017.9

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  • Caポンプのエネルギー共役;M2-helix膜貫通部分(M2m)と細胞質部分(M2c)をつなぐGly105の柔軟性の機能

    ○大保 貴嗣、山崎和生、Stefania Danko、鈴木 裕

    日本生体エネルギー研究会 第42回討論会 

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    Event date: 2016.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Membrane Perturbation of ADP-insensitive Phosphoenzyme of Ca2+-ATPase Modifies Gathering of Transmembrane Helix M2 with Cytoplasmic Domains and Luminal Gating

    ○Stefania Danko, Kazuo Yamasaki, Takashi Daiho, and Hiroshi Suzuki

    日本生体エネルギー研究会 第42回討論会 

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    Event date: 2016.12

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  • 脂質ヘッドグループが筋小胞体カルシウムポンプ活性に及ぼす影響―ナノディスク組み込み標品を用いて―

    ○山崎和生、大保貴嗣、Danko Stefania、鈴木裕

    日本生体エネルギー研究会 第42回討論会 

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    Event date: 2016.12

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  • 筋小胞体Ca2+ポンプM2ヘリックスの膜貫通部分と細胞質部分の連結領域:エネルギー共役における役割

    ○大保 貴嗣、山崎和生、Stefania Danko、鈴木 裕

    第89回日本生化学会大会 

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    Event date: 2016.9

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  • ナノディスクに組み込んだ標品を用いた筋小胞体Ca<SUP>2+</SUP>-ATPaseのEP転換ステップに及ぼす脂質組成及びA23187の影響の解析

    ○山崎和生、大保貴嗣、Danko Stefania、鈴木裕

    第89回日本生化学会大会 

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    Event date: 2016.9

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  • 筋小胞体Ca2+ポンプのエネルギー共役におけるM2 ヘリックス膜貫通部分(M2m)と細胞質部分 (M2cyt)の連結領域の役割

    大保 貴嗣,山崎 和生,Danko Stefania, 鈴木 裕

    日本生体エネルギー研究会 第41回討論会 

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    Event date: 2015.12

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  • ナノディスクへ組み込んだ筋小胞体カルシウムポンプを用いたタンパク質―脂質相互作用の解析

    山崎 和生,大保 貴嗣,Danko Stefania, 鈴木 裕

    日本生体エネルギー研究会 第41回討論会 

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    Event date: 2015.12

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  • 筋小胞体Ca2+ポンプのエネルギー共役におけるM2ヘリックス膜貫通部分(M2m)と細胞質部分 (M2cyt)の連結領域の役割

    大保 貴嗣,山崎 和生,Danko Stefania, 鈴木 裕

    第88回日本生化学会第38回日本分子生物学会合同年会 

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    Event date: 2015.12

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  • 筋小胞体Ca2+-ATPaseのEP転換ステップにおけるTyr122-Hydorophobic clusterの集合過程とエネル ギー共役

    山崎 和生,大保 貴嗣,Danko Stefania, 鈴木 裕

    第88回日本生化学会第38回日本分子生物学会合同年会 

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    Event date: 2015.12

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  • 筋小胞体カルシウムポンプEP転換とカルシウム放出の共役機構におけるTyr122-hydrophobic clusterの役割

    山崎 和生、Stefania Danko、大保 貴嗣、鈴木 裕

    第40回生体エネルギー研究会 

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    Event date: 2014.12

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  • 筋小胞体Ca2+-ATPaseの第2膜貫通へリックス(M2)とロングレンジ共役

    大保 貴嗣、山崎 和生、Stefania Danko、鈴木 裕

    第40回生体エネルギー研究会 

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    Event date: 2014.12

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  • 1分子蛍光観察によるCa2+-ATPaseの数と立体構造変化検出の試み

    小島知樹、戸田啓太、山崎和生、Danko, Stefania、小山洋幸、横田龍一、中山莉奈子、大保貴嗣、鈴木裕、政池知子

    第40回生体エネルギー研究会 

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    Event date: 2014.12

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  • ナノディスクに組み込んだ筋小胞体Ca2+-ATPaseの活性に対する脂質ヘッドグループの影響の解析

    山崎 和生、Stefania Danko、大保 貴嗣、鈴木 裕

    第87回日本生化学 

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    Event date: 2014.10

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  • 筋小胞体Ca2+ポンプのM2へリックス:膜貫通部分と細胞質部分の連結領域の役割

    大保 貴嗣、山崎 和生、Stefania Danko、鈴木 裕

    第87回日本生化学 

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    Event date: 2014.10

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  • Second Transmembrane Helix (M2) and Long-range Coupling in Ca2+-ATPase

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    Event date: 2014.9

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  • A New Method for Ca2+-binding Measurement Applicable to a Small Quantity of Ca2+-ATPase:Transient E2P with Bound Ca2+ and Role of Leu119 on M2 in Ca2+-affinity Reduction and Ca2+ Release. International conference

    Yamasaki K., Danko S., Daiho T. and Suzuki H.

    14th International Conference on P-type ATPases and Related Cation Pumps 

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    Event date: 2014.8 - 2014.9

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  • Long-range Coupling between Catalytic and Transport Sites via Second Transmembrane Helix (M2) in E2P Hydrolysis and E2-E1 Transition of Ca2+-ATPase International conference

    Takashi Daiho, Kazuo Yamasaki, Stefania Danko, and Hiroshi Suzuki

    14th International Conference on P-type ATPases and Related Cation Pumps 

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    Event date: 2014.8 - 2014.9

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  • 筋小胞体Ca2+-ATPaseのE1P-E2PとCa2+放出の共役機構

    山崎和生, 大保貴嗣, Danko Stefania, 鈴木裕

    第38回生体エネルギー研究会 

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    Event date: 2012.12

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  • 筋小胞体Ca2+ポンプの輸送反応における膜貫通ヘリックスM2の役割

    大保貴嗣, Danko Stefania, 山崎和生, 鈴木裕

    第38回生体エネルギー研究会 

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    Event date: 2012.12

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  • 筋小胞体のCa2+ポンプにおけるエネルギー共役;膜貫通へリックスM2およびその細胞質領域アクチュエータードメインとの連結部分の役割

    大保貴嗣, Danko Stefania, 山崎和生, 鈴木裕

    第85回日本生化学会大会 

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    Event date: 2012.12

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  • 筋小胞体Ca2+-ATPaseのE1P-E2PとCa2+放出の共役機構

    山崎和生, 大保貴嗣, Danko Stefania, 鈴木裕

    第85回日本生化学会大会 

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    Event date: 2012.12

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  • 筋小胞体Ca2+ポンプにおけるエネルギー共役;細胞質領域アクチュエータードメインと膜貫通ヘリックスとの連結部分の役割

    大保貴嗣, Danko Stefania, 山崎和生, 鈴木裕

    第37回生体エネルギー研究会 

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    Event date: 2011.12

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  • 筋小胞体Ca2+-ATPaseリン酸化中間体転換ステップにおける静電相互作用の効果とその定量化の試み

    山崎和生, 大保貴嗣, Danko Stefania, 鈴木裕

    第37回生体エネルギー研究会 

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    Event date: 2011.12

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  • Roles of electrostatic interactions between cytoplasmic domains on the phosphoenzyme transition of Ca2+-ATPase and a critical importance of the charged residues at the hinge of the P and N domains International conference

    Kazuo Yamasaki , Takashi Daiho , Stefania Danko , Hiroshi Suzuki

    Na,K-ATPase and Related P-ATPases: Structure, Biology and Medicin e 

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    Event date: 2011.9 - 2011.10

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  • Critical importance of helix-loop conversions, length, and Actuator- domain junction structure of M2 in Ca2+-ATPase for Ca2+ transport International conference

    Takashi Daiho , Stefania Danko , Kazuo Yamasaki , Hiroshi Suzuki

    Na,K-ATPase and Related P-ATPases: Structure, Biology and Medicin e 

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    Event date: 2011.9 - 2011.10

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  • Mechanism of Ca2+-ATPase revealed by mutations, kinetics, and development of stable analogs of phosphorylated intermediates International conference

    Hiroshi Suzuki , Takashi Daiho, Kazuo Yamasaki , Stefania J. Danko

    Na,K-ATPase and Related P-ATPases: Structure, Biology and Medicin e 

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    Event date: 2011.9 - 2011.10

    Language:English   Presentation type:Oral presentation (invited, special)  

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  • 筋小胞体Ca2+-ATPaseのリン酸化中間体の異性化と加水分解に影響を及ぼす構造因子

    ○大保 貴嗣,ダンコ ステファニア,山崎 和生,鈴木 裕

    第83回日本生化学会第33回日本分子生物学会年会合同大会 

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    Event date: 2010.12

    Language:Japanese   Presentation type:Poster presentation  

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  • 筋小胞体Ca2+-ATPaseによるCa2+輸送;細胞質領域アクチュエータードメインお膜貫通へリックスを結ぶループの役割

    ○大保 貴嗣、Danko Stefania、山崎 和生、鈴木 裕

    生体エネルギー研究会第36回討論会 

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    Event date: 2010.11

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 筋小胞体Ca2+-ATPaseの反応サイクルにおける静電的相互作用の役割とイオン強度依存性との関連

    山崎 和生、大保 貴嗣、Danko Stefania、鈴木 裕

    生体エネルギー研究会第36回討論会 

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    Event date: 2010.11

    Language:Japanese   Presentation type:Poster presentation  

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  • 筋小胞体Ca2+-ATPaseのリン酸化中間体転換とCa2+輸送;細胞質領域アクチュエータードメインお膜貫通へリックスを結ぶループの役割

    ○大保 貴嗣、Danko Stefania、山崎 和生、鈴木 裕

    生体エネルギー研究会第35回討論会 

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    Event date: 2009.12

    Language:Japanese   Presentation type:Poster presentation  

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  • 筋小胞体Ca2+-ATPaseのE1P-E2P転換とCa2+共役におけるK+の役割

    山崎 和生、大保 貴嗣、鈴木 裕

    生体エネルギー研究会第35回討論会 

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    Event date: 2009.12

    Language:Japanese   Presentation type:Poster presentation  

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  • 筋小胞体Ca2+-ATPaseのE1P-E1P転換とCa2+共役におけるK+の役割

    山崎 和生、大保 貴嗣、鈴木 裕

    第82回日本生化学会大会 

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    Event date: 2009.10

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 高濃度ATPによる筋小胞体リン酸化中間体分解の促進効果について;Actuator/M1-linkerを伸長させた変異体を用いた解析

    大保 貴嗣、山崎 和生、ダンコ ステファニア、鈴木 裕

    第82回日本生化学会大会 

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    Event date: 2009.10

    Language:Japanese   Presentation type:Poster presentation  

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  • 筋小胞体Ca2+-ATPaseのE2PCa2におけるTyr122-Hydrophobic clusterの果たす役割について

    山崎 和生、王 国麗、大保 貴嗣、鈴木 裕

    第81回日本生化学会第31回日本分子生物学会年会合同大会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Poster presentation  

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  • 筋小胞体Ca2+-ATPaseのCa2+結合型-リン酸化中間体アナログの安定性に及ぼすヌクレオチドの効果

    大保 貴嗣、Stefania Danko、山崎 和生、鈴木 裕

    第81回日本生化学会第31回日本分子生物学会年会合同大会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Roles of interactions between Actuator- and Nucleotide Binding- domains of Ca2+-ATPasereticulum Ca2+-ATPase

    Xiaoyu Ryu, Takashi Daiho, Kazuo Yamasaki, Stefania Danko, and Hiroshi Suzuki

    第81回日本生化学会第31回日本分子生物学会年会合同大会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Poster presentation  

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  • 筋小胞体Ca2+-ATPaseのリン酸化中間体の転換及びCa2+輸送におよぼすヌクレオチドの顕著な効果

    ○大保 貴嗣、山崎 和生、鈴木 裕

    生体エネルギー研究会第34回討論会 

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    Event date: 2008.11

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Ca2+ポンプの輸送機構―部位特異的変異およびリン酸化中間体アナログの開発と構造解析による理解

    ○鈴木 裕、大保 貴嗣、山崎 和生、劉 暁宇、Danko Stefania

    生体エネルギー研究会第34回討論会 

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    Event date: 2008.11

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

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  • 筋小胞体Ca2+-ATPaseのP-Nヒンジ領域に存在する荷電側鎖の役割と一価イオンの影響

    山崎 和生、大保 貴嗣、鈴木 裕

    生体エネルギー研究会第34回討論会 

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    Event date: 2008.11

    Language:Japanese   Presentation type:Poster presentation  

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  • Critical Role of Glu40–Ser48 Loop Linking Actuator Domain and First Transmembrane Helix of Ca2+-ATPase in Ca2+ Deocclusion and Release from ADP-insensitive Phosphoenzyme International conference

    ○Takashi Daiho, Kazuo Yamasaki, Stefania Danko, Hiroshi Suzuki

    12 th International ATPase ConferenceNa,K-ATPase and Related Transport ATPases of P-type: Structures, Mechanisms, and Roles in Health and Disease 

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    Event date: 2008.8

    Language:English   Presentation type:Poster presentation  

    Venue:Aarhus, (Denmark)  

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  • Roles of the A domain and its linkers of sarco(endo)plasmic reticulum Ca2+ pump for processing the phosphorylated intermediates and Ca2+ release International conference

    ○HIroshi Suzuki, Takashi Daiho, Kazuo Yamasaki, Xiaoyu Liu, and Stefania Danko

    12 th International ATPase ConferenceNa,K-ATPase and Related Transport ATPases of P-type: Structures, Mechanisms, and Roles in Health and Disease 

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    Event date: 2008.8

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

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  • Roles of Tyr122-Hydrophobic cluster and K+ binding in Ca2+-releasing Process of ADP-insensitive Phosphoenzyme of Sarcoplasmic Reticulum Ca2+-ATPase International conference

    Kazuo Yamasaki, Guoli Wang, Takashi Daiho, and Hiroshi Suzuki

    12 th International ATPase ConferenceNa,K-ATPase and Related Transport ATPases of P-type: Structures, Mechanisms, and Roles in Health and Disease 

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    Event date: 2008.8

    Language:English   Presentation type:Poster presentation  

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  • Critical Role of Glu40?Ser48 Loop Linking Actuator Domain and First Transmembrane Helix of Ca2+-ATPase in Ca2+ Deocclusion and Release from ADP-insensitive Phosphoenzyme International conference

    ○Takashi Daiho, Kazuo Yamasaki, Stefania Danko, and Hiroshi Suzuki

    12 th International ATPase ConferenceNa,K-ATPase and Related Transport ATPases of P-type: Structures, Mechanisms, and Roles in Health and Disease 

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    Event date: 2008.8

    Language:English   Presentation type:Poster presentation  

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  • 筋小胞体Ca2+-ATPaseの変異体によるCa2+を閉塞したADP非感受性リン酸化中間体のアナログの形成

    大保 貴嗣、Stefania Danko、山崎 和生、鈴木 裕

    第80回日本生化学会第30回日本分子生物学会年会合同大会 

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    Event date: 2007.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 筋小胞体Ca2+-ATPaseのCa2+放出過程におけるTyr122-Hydrophobic clusterとK+の関連

    山崎 和生、王 国麗、大保 貴嗣、鈴木 裕

    第80回日本生化学会第30回日本分子生物学会年会合同大会 

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    Event date: 2007.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Role of the region linking cytoplasmic and transmembrane domains in processing of phosphorylated intermediates for Ca2+ transport of sarcoplasmic International conference

    ○Takashi Daiho, Kazuo Yamasaki, Stefania Danko, and Hiroshi Suzuki

    20th IUBMB Interbational Congress of Biochemistry and Molecular Biology and11th FAOBMB Congress 

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    Event date: 2006.6

    Language:English   Presentation type:Poster presentation  

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  • The Ca2+ bound ADP insensitive Phosphoenzyme of Sarcoplasmic Reticulum Ca2+-ATPase Mutant International conference

    Kazuo Yamasaki, Guoli Wang, Takashi Daiho, and Hiroshi Suzuki

    20th IUBMB Interbational Congress of Biochemistry and Molecular Biology and11th FAOBMB Congress 

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    Event date: 2006.6

    Language:English   Presentation type:Poster presentation  

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  • Molecular Mechanism of Ca2+-ATPase and Darier Disease Caused by Its defects International conference

    ○Hiroshi Suzuki, Takashi Daiho, Kazuo Yamasaki, and Stefania Danko

    “Molecular Mechanism and Regulation in Cation Transport ATPases and Related Genetic Diseases” Satellite meeting for 20th IUBMB International Congress of Biochemistry and Molecular Biology & 11th FAOBMB Congress 

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    Event date: 2006.6

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

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  • Critical Role of the A-domain/M1 Linker of Ca2+-ATPase in Ca2+ Release into Lumen from ADP-insensitive Phosphoenzyme with Occluded Ca2+ International conference

    ○Takashi Daiho, Kazuo Yamasaki, Stefania Danko, and Hiroshi Suzuki

    “Molecular Mechanism and Regulation in Cation Transport ATPases and Related Genetic Diseases” Satellite meeting for 20th IUBMB International Congress of Biochemistry and Molecular Biology & 11th FAOBMB Congress 

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    Event date: 2006.6

    Language:English   Presentation type:Oral presentation (general)  

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  • Critical Role of Tyr122 in Ca2+-release from ADP-insensitive Phosphoenzyme of Sarcoplasmic Reticulum Ca2+-ATPase International conference

    Kazuo Yamasaki, Guoli Wang, Takashi Daiho, and Hiroshi Suzuki

    “Molecular Mechanism and Regulation in Cation Transport ATPases and Related Genetic Diseases” Satellite meeting for 20th IUBMB International Congress of Biochemistry and Molecular Biology & 11th FAOBMB Congress 

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    Event date: 2006.6

    Language:English   Presentation type:Oral presentation (general)  

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  • 筋小胞体Ca2+-ATPaseリン酸化中間体の輸送部位におけるCa2+閉塞/脱閉塞の部位特異的変異による研究

    ○大保 貴嗣、山崎 和生、鈴木 裕

    第78回 日本生化学会大会 

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    Event date: 2005.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

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  • 筋小胞体Ca2+-ATPaseリン酸化中間体からのCa2+放出過程の部位特異的変異による解析

    ○山崎 和生、王 国麗、大保 貴嗣、鈴木 裕

    第78回 日本生化学会大会 

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    Event date: 2005.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

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  • 筋小胞体CaポンプのTyr122残基を中心とした疎水性相互作用の重要性

    山崎 和生、大保 貴嗣、王 国麗、鈴木 裕

    生体エネルギー研究会第30回討論会 

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    Event date: 2004.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 小胞体Caポンプ分子異常によるダリエー病

    ○鈴木 裕、山崎 和生、大保 貴嗣、佐藤 克彦、宮内 勇貴、高橋 英俊、山本 明美、中村 哲史、Danko Stefania、飯塚 一

    生体エネルギー研究会第30回討論会 

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    Event date: 2004.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 筋小胞体Ca2+-ATPaseのP-Aドメイン間に生じる疎水的相互作用はエネルギー共役に必須である

    ○王 国麗、山崎 和生、大保 貴嗣、Danko Stefania、鈴木 裕

    第77回 日本生化学会大会 

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    Event date: 2004.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

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  • メタルフッ素化合物の利用により明らかにされた筋小胞体Ca2+-ATPaseリン酸化中間体の加水分解に伴う触媒部位と輸送部位における構造変化

    ○鈴木 裕、Danko Stefania、山崎 和生、大保 貴嗣

    第77回 日本生化学会大会 

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    Event date: 2004.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

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  • ダリエー病患者で見つかった51種のSERCA2bミスセンス変異体における機能と発現の包括的及び体系的解析

    ○大保 貴嗣、宮内 勇貴、山崎 和生、佐藤 克彦、高橋 英俊、山本 明美、中村 哲史、飯塚 一、鈴木 裕

    第77回 日本生化学会大会 

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    Event date: 2004.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

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  • ダリエー病を引き起こすSERCA2b変異体の反応速度論的解析

    山崎 和生、佐藤 克彦、大保 貴嗣、宮内 勇貴、高橋 英俊、山本 明美、中村 哲史、飯塚 一、鈴木 裕

    第77回 日本生化学会大会 

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    Event date: 2004.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

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  • 筋小胞体Ca2+-ATPaseのマグネシウム・フッ素複合体はCa2+非存在下でのC12E8による可溶化に対して安定である

    山崎 和生, 大保 貴嗣, 上堂地 美佳, 鈴木 裕

    生化学  2000.8 

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  • 筋小胞体カルシウムポンプのナノディスクへの組み込みとその性質

    山崎 和生, Danko Stefania, 大保 貴嗣, 鈴木 裕

    日本生化学会大会プログラム・講演要旨集  2013.9 

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  • 筋小胞体Ca2+-ATPaseのE1P-E2P転換とCa2+輸送の共役におけるK+の役割

    山崎 和生, 大保 貴嗣, 鈴木 裕

    日本生化学会大会プログラム・講演要旨集  2009.9 

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  • 筋小胞体Ca2+-ATPaseのリン酸化中間体転換過程における静電的相互作用の役割

    山崎 和生, 大保 貴嗣, Danko Stefania, 鈴木 裕

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2010.12 

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  • 筋小胞体Ca2+-ATPaseのE1P-E2P転換とCa2+放出の共役機構

    山崎 和生, 大保 貴嗣, Danko Stefania, 鈴木 裕

    日本生化学会大会プログラム・講演要旨集  2012.12 

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  • 筋小胞体Ca2+-ATPaseのCa2+放出過程におけるTyr122-Hydrophobic clusterとK+との関連

    山崎 和生, 王 国麗, 大保 貴嗣, 鈴木 裕

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2007.11 

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  • フェニレンビスマレイミドによる筋小胞体Ca-ATPaseの分子間架橋

    山崎 和生

    生化学  1988.8 

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  • フェニレンビスマレイミドによる筋小胞体Ca-ATPaseの分子間架橋

    山崎 和生

    生化学  1989.9 

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  • 骨格筋小胞体Ca-ATPaseのE2P形成のVanadateによる阻害

    山崎 和生

    生化学  1991.8 

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  • 筋小胞体Ca2+-ATPaseのFDNP-ATPによる親和修飾と修飾残基の同定

    山崎 和生

    生化学  1993.8 

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  • 筋小胞体Ca2+-ATPaseのテトラニトロメタンによる修飾と活性阻害-活性に関与するチロシン残基の検索

    山崎 和生

    生化学  1994.7 

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  • Ca2+非存在下での可溶化筋小胞体Ca2+-ATPaseのATP,ADP,及びMg2+による安定化とイオン強度の影響

    山崎 和生, 大保 貴嗣, 鈴木 裕

    生化学  2001.8 

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  • 筋小胞体Ca2+-ATPaseの細胞質ドメインに存在するイオンペアの役割

    山崎 和生, 大保 貴嗣, 鈴木 裕

    生化学  2002.8 

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  • 筋小胞体Ca2+-ATPaseのHis-5のジエチルピロカーボネートによる修飾はリン酸化中間体の加水分解をブロックする

    山崎 和生

    生化学  1997.7 

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Awards

  • 12 th International ATPase Conference: Na,K-ATPase and Related Transport ATPases of P-type: Structures, Mechanisms, and Roles in Health and Disease:Margrethe Moller awards for best poster

    2008.8   International ATPase Conference Na,K-ATPase and Related Transport ATPases of P-type: Structures,  

    Kazuo Yamasaki

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    Award type:Award from international society, conference, symposium, etc.  Country:Denmark

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Research Projects

  • Exploration of uncoupling mode of sarcoplasmic reticulum Ca2+ pump

    Grant number:21K06058  2021.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount:\4,160,000 ( Direct Cost: \3,200,000 、 Indirect Cost:\960,000 )

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  • Effects of lipid envionment on the functions of sarcoplasmic reticulum calcium pump

    Grant number:18K06105  2018.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Kazzuo Yamasaki

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    Grant amount:\4,160,000 ( Direct Cost: \3,200,000 、 Indirect Cost:\960,000 )

    Sarcoplasmic reticulum Ca pump was used to investigate the effect of transient protein-lipid interactions on the function of membrane proteins. The results show that the interaction between Arg324 and the phosphate group of the phospholipid, which wasobserved in the crystal structure, plays an important role in determining the affinity for lumenal Ca in the calcium release process of the calcium pump. It was also found that Arg324 changes partners with multiple types of lipids during the reaction cycle. It was concluded that in order to fully understand the function of the calcium pump under physiological conditions, it is essential to examine its behavior in a complex lipid environment rather than a single lipid environment.

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  • P-type pump ; Molecular basis of energy coupling and carcinogenesis caused by defects

    Grant number:15H04346  2015.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Suzuki Hiroshi, MASAIKE Tomoko, MINOWA Osamu, DANKO Stefania

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    Grant amount:\16,250,000 ( Direct Cost: \12,500,000 、 Indirect Cost:\3,750,000 )

    Gating mechanism of sarco(endo)plasmic reticulum Ca-pump SERCA1a and involvement of lipid on membrane in the pump function were studied, thereby understanding of energy coupling mechanism in the active Ca2+ transport were further deepened. By the use of the Ca2+-pump, the methods for a single molecule dynamics were developed, and the structural changes of the pump were analyzed for understanding the structural mechanism. For the flippase, another P-type ATPase, studies were performed to identify its specific substrates, and reveal the mechanism. Fur types of mutations of endoplasmic Ca2+-pump SERCA2b that develop squamous cell carcinoma in mouse model were identified, and the pathological consequences and defects in the pump protein were revealed.

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  • Exploration into the effects of lipid envionment on the functions of sarcoplasmic reticulum calcium pump

    Grant number:15K06988  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YAMASAKI Kazuo

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    Grant amount:\5,070,000 ( Direct Cost: \3,900,000 、 Indirect Cost:\1,170,000 )

    To explore the effects of lipid environment on the function of sarcoplasmic reticulum Ca2+-ATPase, I tried to embed the Ca2+-ATPase into small lipid-bilayer (nanodiscs) with various lipid components. The Ca2+-ATPase was successfully embedded into the nanodisc without loss of its activities and the Ca2+-ATPase-embedded nanodiscs were utilized for various analyses. Subsequently, the contributions of lipid environments on the enzymatic activities were estimated quantitively by systematic analyses and could be distinguished between components of electrostatic- and steric-interactions. Furthermore, the results indicated that surface charges, but not particular lipid-head group, directly modulate the functions of Ca2+-ATPase. This finding is possibly important for not only Ca2+-ATPase but also for other functional membrane proteins.

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  • Analysis of the effects of lipid environment and roles of electrostatic interactions on the Ca2+-ATPase function

    Grant number:24570147  2012.4 - 2015.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YAMASAKI Kazuo

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    Grant amount:\5,330,000 ( Direct Cost: \4,100,000 、 Indirect Cost:\1,230,000 )

    The role of electrostatic interactions in and the effects of the lipid environment on the reaction mechanisms on sarcoplasmic reticulum Ca2+-ATPase were explored. The experiments using the Ca2+-ATPase buried into nanodiscs which contained various lipid contents revealed that the not only electrostatic interactions between protein and lipids but also steric interactions between them have important for function of the pump. This study succeeded clearly to divide the effects of the lipid environment between electrostatic interactions and steric interactions.

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  • Molecular basis of P-type ion pumps and diseases caused by pump defects.

    Grant number:23370058  2011.4 - 2015.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    SUZUKI Hiroshi, YAMASAKI Kazuo, DAIHO Takashi

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    Grant amount:\19,890,000 ( Direct Cost: \15,300,000 、 Indirect Cost:\4,590,000 )

    P-type ATPases possess common domain-structure and couple the ATP hydrolysis with the specific cation-transport. In this study, we first revealed by extensive mutations and kinetic analyses of Ca-ATPase that the second transmembrane helix plays critical roles via its motions and changes in its secondary structure for transferring the motions of cytoplasmic domains to the transmembrane cation transport sites there by for the energy coupling. We also revealed that unique structural fluctuation is equipped in each of the phosphoenzyme intermediates, and the fluctuation functions to accomplish the proper forward transport reaction. Thus we contributed to the comprehensive understanding of the P-type ATPase mechanism and an establishment of molecular basis for diseases developed by the pump defects.

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  • Analysis of the role of electrostatic interactions between cytoplasmic domains of sarcoplasmic reticulum calcium pump

    Grant number:21570127  2009.4 - 2012.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YAMASAKI Kazuo

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    Grant amount:\4,550,000 ( Direct Cost: \3,500,000 、 Indirect Cost:\1,050,000 )

    I explored the roles of electrostatic interactions in the movement of cytoplasmic domain of sarcoplasmic reticulum calcium pump by using a kinetic analysis of site-directed mutants. In this study, I found a linear relationship between logarithm of rate constant and square of the activity coefficient. This plot revealed that electrostatic interactions between domains act like a guide line of domain motion. This plot would provide a useful tool for quantification of electrostatic effects in the enzyme reactions.

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  • Molecular Mechanism of Endoplasmic and Golgi Cation Pumps and Diseases Caused by Their Defects

    Grant number:18370058  2006.4 - 2009.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    SUZUKI Hiroshi, DAIHO Takashi, YAMASAKI Kazuo

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    Grant amount:\17,540,000 ( Direct Cost: \15,200,000 、 Indirect Cost:\2,340,000 )

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  • Analysis of the Ca^<2+> release mechanism from the E2PCa intermediate of sarcoplasmic reticulum Ca^<2+> pump

    Grant number:18570119  2006.4 - 2008.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YAMASAKI Kazuo

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    Grant amount:\3,890,000 ( Direct Cost: \3,500,000 、 Indirect Cost:\390,000 )

    Development of method for measurement of Ca^<2+> binding to mutants of Ca^<2+> -ATPase, and improvement of its accuracy
    Measurement of bound Ca^<2+> was enabled to be done directly by devising the washing solution of the membrane filtoration method in this research, though it was very difficult in the analysis that used the mutant of sarcoplasmic reticulum Ca^<2+>-ATPase to measure Ca^<2+> binding from the low degree of the amount of appearance so far.
    Analysis of Ca^<2+> releasing process that uses mutants
    In mutant that changes Tyr^<122> of sarcoplasmic reticulum Ca^<2+>-ATPase into Ala (Y122A), I have already found that the E2PCa intermediate accumulates during it in the steady state. In crystal structures of E2P analogs, Tyr^<122> located at the center of the hydrophobic interaction network (Tyr^<122> Hydrophobic cluster: Y122-HC) which situated between cytoplasm domains. The Ala substitution mutants of the other residues include in Y122-HC were made and analyzed its kinetics of Ca^<2+> releasing step. It was revealed from this analysis that the rates of Ca^<2+> release toward lumen side and of association had decreased remarkably compared with a wild type in each mutant. Moreover, the accumulation of the E2PCa in the steady state was observed in a part of mutants as well as Y122A. When Ca^<2+> release from the phosphorylation medium was measured directly by using the above-mentioned method about L119A in this mutant, it was shown that Ca^<2+> release from E2PCa^<2+> was obviously slower than the conversion of the phosphoenzyme (E1PCa_2⇒E2PCa_2). The role of Y122-HC in Ca^<2+> transport of Ca^<2+>-ATPase became clear from the above-mentioned result.
    Analysis that uses wild type Ca^<2+>-ATPase
    In a series of analysis, it was newly clarified to wild type Ca^<2+>-ATPase that the character to Ca^<2+> of E2P resembled the Y122-HC mutant very much in the absence of K^+. The result that K^+ ion plays an important role to Ca^<2+> release process of Ca^<2+>-ATPase is a finding of an important for recognize the Ca^<2+> transport mechanism of Ca^<2+>-ATPase.

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  • Study on Molecular Mechanism of Calcium Pump of Sarcoplasmic Reticulum

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    Grant type:Competitive

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  • 筋小胞体カルシウムポンプの分子機構の研究

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    Grant type:Competitive

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  • 筋小胞体Ca2+-ATPaseの機能構造連関

    基礎科学研究 

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  • 旭川医大39派遣講座

    2019.10

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    「お堅い『酵素』の話~『酵素』って何なの~」

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