2025/03/11 更新

写真a

ハルミ タツオ
春見 達郎
HARUMI Tatsuo
所属
医学部 医学科 基礎医学講座 解剖学講座(顕微解剖学分野)
外部リンク

学位

  • 学術博士 ( 1991年3月   金沢大学 )

  • 理学修士

  • 学術博士

研究分野

  • ライフサイエンス / 形態、構造

学歴

  • 金沢大学

    - 1991年

      詳細を見る

  • 金沢大学   自然科学研究科   生命科学専攻

    - 1991年

      詳細を見る

    国名: 日本国

    researchmap

所属学協会

論文

  • Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish Girella punctata 査読

    Muhammad Ahya Rafiuddin, Hajime Matsubara, Kaito Hatano, Masato Honda, Kenji Toyota, Kouhei Kuroda, Keito Tsunoda, Yukihiro Furusawa, Yoshiaki Tabuchi, Tetsushi Hirano, Akihiro Sakatoku, Chun-Sang Hong, Ajai K. Srivastav, Thumronk Amornsakun, Nobuaki Shimizu, Mohamed I. Zanaty, Tatsuo Harumi, Kohei Yamauchi, Tamás Müller, Ning Tang, Atsuhiko Hattori, Kazuichi Hayakawa, Nobuo Suzuki

    Toxics   12 ( 12 )   915 - 915   2024年12月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    Polycyclic aromatic hydrocarbons (PAHs) are known to have toxic effects on fish. In this study, we examined the effects of benz[a]anthracene (BaA), a type of PAH, on fish liver metabolism. Nibbler fish (Girella punctata) were intraperitoneally injected with BaA (10 ng/g body weight) four times over a 10-day period. BaA significantly decreased known bone metabolism-related plasma factors such as calcium and inorganic phosphorus. Moreover, significant reductions were observed in the plasma levels of known liver metabolism-related factors, including ferrous ions, total bile acids, total bilirubin, free bilirubin, aspartate aminotransferase, and alkaline phosphatase. Interestingly, mono-hydroxylated metabolites of BaA, such as 3 hydroxylbenz[a]anthracene (3-OHBaA), were detected in the bile of BaA-injected nibbler fish. This hydroxylated form of BaA was found in its free form, rather than conjugated with glucuronic acid or sulfuric acid. Due to the lack of whole-genome sequence data for the nibbler fish, two nibbler fish-specific apoptosis-related factors (TNF receptor superfamily member 1A: tnfrsf1a and TNF superfamily member 10: tnfsf10) were isolated by De novo RNA sequencing. In a liver tissue culture, 3-OHBaA (10−6 M) significantly upregulated the expression of tnfrsf1a and tnfsf10 in the liver. These results provide the first evidence that 3-OHBaA metabolites exhibit toxic effects on the liver in teleost.

    DOI: 10.3390/toxics12120915

    researchmap

  • Fluorene is Highly Toxic to Zoea Larvae of the Red-Clawed Crab Chiromantes haematocheir 査読

    Kawamura Ryoya 他

    International Journal of Zoological Investigations   9 ( 1 )   1 - 7   2023年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.33745/ijzi.2023.v09i01.001

  • Fluorene is Highly Toxic to Zoea Larvae of the Red-Clawed Crab Chiromantes haematocheir 査読

    Kawamura Ryoya, Rafiuddin Ahya Muhammad, Toyota Kenji, Honda Masato, Amornsakun Thumronk, Tabuchi Yoshiaki, Hattori Atsuhiko, Harumi Tatsuo, Hirayama Jun, Urata Makoto, Matsumoto Kyoko, Nishimoto Sogo, Ajai K Srivastav, Nobuo Suzuki, Matsubara Hajime

    International Journal of Zoological Investigations   9 ( 1 )   1 - 7   2023年

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Saran Publications  

    DOI: 10.33745/ijzi.2023.v09i01.001

    researchmap

  • Chemical and physical guidance of fish spermatozoa into the egg through the micropyle 査読

    Ryuzo Yanagimachi, Tatsuo Harumi, Hajime Matsubara, Wei Yan, Shuiqiao Yuan, Noritaka Hirohashi, Tomohiro Iida, Etsuro Yamaha, Katsutoshi Arai, Takahiro Matsubara, Tadashi Andoh, Carol Vines, Gary N. Cherr

    Biology of Reproduction   96 ( 4 )   780 - 799   2017年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Society for the Study of Reproduction  

    Eggs of teleost fish, unlike those of many other animals, allow sperm entry only at a single site, a narrow canal in the egg's chorion called the micropyle. In some fish (e.g., flounder, herring, and Alaska pollock), the micropyle is a narrow channel in the chorion, with or without a shallow depression around the outer opening of micropyle. In some other fish (e.g., salmon, pufferfish, cod, and medaka), the micropyle is like a funnel with a conical opening. Eggs of all the above fish have a glycoprotein tightly bound to the chorion surface around the micropyle. This glycoprotein directs spermatozoa into the micropylar canal in a Ca2+-dependent manner. This substance, called the micropylar sperm attractant or MISA, increases fertilization efficiency and is essential in herring. In flounder, salmon, and perhaps medaka, fertilization is possible without MISA, but its absence makes fertilization inefficient because most spermatozoa swim over the micropyle without entering it. The mechanism underlying sperm-MISA interactions is yet to be determined, but at least in herring the involvement of Ca2+ and K+ channel proteins, as well as CatSper and adenylyl cyclase, is very likely. In some other fish (e.g., zebrafish, loach, and goldfish), the chorion around the micropyle is deeply indented (e.g., zebrafish and loach) or it has radially or spirally arranged grooves around the outer opening of the micropyle (e.g., goldfish). MISA is absent from the eggs of these fish and sperm entry into micropylar canal seems to be purely physical.

    DOI: 10.1093/biolre/iox015

    Scopus

    PubMed

    researchmap

  • Sperm Attractant in the Micropyle Region of Fish and Insect Eggs1 査読

    Ryuzo Yanagimachi, Gary Cherr, Takahiro Matsubara, Tadashi Andoh, Tatsuo Harumi, Carol Vines, Murali Pillai, Frederick Griffin, Hajime Matsubara, Tina Weatherby, Kenneth Kaneshiro

    Biology of Reproduction   88 ( 2 )   2013年2月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1095/biolreprod.112.105072

    researchmap

  • Tubular Injury in a Rat Model of Type 2 Diabetes Is Prevented by Metformin: A Possible Role of HIF-1α Expression and Oxygen Metabolism

    Y Takiyama, T Harumi, J Watanabe, Y Fujita, J Honjo, N Shimizu, Y Makino and M Haneda

    Diabetes   60   981 - 992   2011年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2337/db10-0655

  • Tubular injury in a rat model of type 2 diabetes is prevented by metformin: A possible role of HIF-1α expression and oxygen metabolism 査読

    Yumi Takiyama, Tatsuo Harumi, Jun Watanabe, Yukihiro Fujita, Jun Honjo, Norihiko Shimizu, Yuichi Makino, Masakazu Haneda

    Diabetes   60 ( 3 )   981 - 992   2011年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE - Chronic hypoxia has been recognized as a key regulator in renal tubulointerstitial fibrosis, as seen in diabetic nephropathy, which is associated with the activation of hypoxia-inducible factor (HIF)-1α. We assess here the effects of the biguanide, metformin, on the expression of HIF-1α in diabetic nephropathy using renal proximal tubular cells and type 2 diabetic rats. RESEARCH DESIGN AND METHODS - We explored the effects of metformin on the expression of HIF-1α using human renal proximal tubular epithelial cells (HRPTECs). Male Zucker diabetic fatty (ZDF
    Gmi-fa/fa) rats were treated from 9 to 39 weeks with metformin (250 mg · kg-1 · day-1) or insulin. RESULTS - Metformin inhibited hypoxia-induced HIF-1α accumulation and the expression of HIF-1-targeted genes in HRPTECs. Although metformin activated the downstream pathways of AMP-activated protein kinase (AMPK), neither the AMPK activator, AICAR, nor the mTOR inhibitor, rapamycin, suppressed hypoxia-induced HIF-1α expression. In addition, knockdown of AMPK-α did not abolish the inhibitory effects of metformin on HIF-1α expression. The proteasome inhibitor, MG-132, completely eradicated the suppression of hypoxia-induced HIF-1α accumulation by metformin. The inhibitors of mitochondrial respiration similarly suppressed hypoxia-induced HIF-1α expression. Metformin significantly decreased ATP production and oxygen consumption rates, which subsequently led to increased cellular oxygen tension. Finally, metformin, but not insulin, attenuated tubular HIF-1α expression and pimonidazole staining and ameliorated tubular injury in ZDF rats. CONCLUSIONS - Our data suggest that hypoxia-induced HIF-1α accumulation in diabetic nephropathy could be suppressed by the antidiabetes drug, metformin, through the repression of oxygen consumption. © 2011 by the American Diabetes Association.

    DOI: 10.2337/db10-0655

    Scopus

    PubMed

    researchmap

  • Measurement of melatonin in body fluids: Standards, protocols and procedures

    E.A. de Almeida, P. Di Mascio, T. Harumi, D.S. Spence, A. Moscovitch, R. Hardeland, D.P. Cardinali

    Childs Nerv. Syst.   Online 21 Nov.   2010年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • The use of poly-L-lysine to facilitate examination of sperm entry into pelagic, non-adhesive fish eggs. 招待 査読

    T. Andoh, T. Matsubara, T. Harumi and R. Yanagimachi

    Int. J. Dev. Biol.   52   753 - 757   2008年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1387/ijdb.072543ta

  • The use of poly-L-lysine to facilitate examination of sperm entry into pelagic, non-adhesive fish eggs 査読

    Tadashi Andoh, Takahiro Matsubara, Tatsuo Harumi, Ryuzo Yanagimachi

    International Journal of Developmental Biology   52 ( 5-6 )   753 - 757   2008年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The fish egg is surrounded by a thick envelope called the chorion. The fertilizing spermatozoon enters the egg through a canal-like structure in the chorion, the micropyle. Examination of micropyle at fertilization is difficult if eggs are large and have no distinct landmarks surrounding the micropyle, or if they are positively buoyant in water. Eggs of many commercially important fishes (e.g., flounder, sea bream and eel) are buoyant in water or only slightly adhere to solid objects (e.g., sands, rock and water plants), which makes observation of spermatozoa at fertilization difficult. Here, we report that such eggs can be firmly attached to plastic and glass dishes that have been previously coated with poly-L-lysine. These adhering eggs can be fertilized and develop normally on the dishes. Observations of micropyles of three fish species, before and after sperm entry are presented. © UBC Press.

    DOI: 10.1387/ijdb.072543ta

    Scopus

    PubMed

    researchmap

  • Chromatographic Analyses of Biogenic Melatonin and Its Related Indoleamines

    T. Harumi, S. R. Pandi-Perumal, G. R. Martinez, P. Di Mascio, and Y. Hayashi

    in "Melatonin: From Molecules to Therapy"   3 - 25   2007年4月

     詳細を見る

    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • 疾患モデルマウスにおける体外受精率の比較-系統差-

    清水範彦、早川寿行、春見達郎、中谷和宏

    日本実験動物技術者協会北海道支部会誌   ( 28-29 )   34 - 35   2005年4月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Immunocytochemical localization of secretogranin III in the endocrine pancreas of male rats

    Y. Sakai, M. Hosaka, Y. Hira, T. Harumi, A. Yoshinaga and T. Watanabe

    Archives of Histology and Cytology   67   54 - 64   2004年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1679/aohc.67.57

  • Immunocytochemical localization of secretogranin III in the endocrine pancreas of male rats 査読

    Yuko Sakai, Masahiro Hosaka, Akihiro Yoshinaga, Yoshiki Hira, Tatsuo Harumi, Tsuyoshi Watanabe

    Archives of Histology and Cytology   67 ( 1 )   57 - 64   2004年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Secretogranin HI (SgIII), a member of the granin protein family, is expressed specifically in neuronal and endocrine cells. To examine the precise localization of SgIII in the endocrine pancreas, pancreatic tissues of rats were analyzed immunocytochemically with a polyclonal anti-serum raised against rat SgIII. By light microscopy of semithin sections, the immunoreactivity for SgIII was readily detected in pancreatic A- and B-cells, faintly so in D-cells, and not at all in the exocrine pancreas. By immunoelectron microscopy, immunogold particles indicative of SgIII were observed in the peripheral regions of secretory granules, and universally in the pancreatic endocrine cells. Morphometrical analyses indicated that SgIII is most preferentially localized in the periphery of the secretory granule among granins. These findings suggest that SgIII is closely associated with the secretory granule membrane, serving to anchor the aggregates of other soluble constituents to the membrane.

    DOI: 10.1679/aohc.67.57

    Scopus

    PubMed

    researchmap

  • Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands 査読

    Yuko Sakai, Masahiro Hosaka, Yoshiki Hira, Tatsuo Harumi, Yoshiyuki Ohsawa, Hua Wang, Toshiyuki Takeuchi, Yasuo Uchiyama, Tsuyoshi Watanabe

    Journal of Histochemistry and Cytochemistry   51 ( 2 )   227 - 238   2003年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Histochemical Society Inc.  

    Secretogranin III (SgIII) is one of the acidic secretory proteins, designated as granins, which are specifically expressed in neuronal and endocrine cells. To clarify its precise distribution in the anterior lobe of the rat pituitary gland, we raised a polyclonal antiserum against rat SgIII for immunocytochemical analyses. By immunohistochemistry using semithin sections, positive signals for SgIII were detected intensely in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, but not in somatotropes. The distribution pattern of SgIII in the pituitary gland was similar to that of chromogranin B (CgB), also of the granin protein family, suggesting that the expressions of these two granins are regulated by common mechanisms. The localization of SgIII in endocrine cells was confirmed by immunoelectron microscopy. In particular, secretory granules of mammotropes and thyrotropes were densely and preferentially co-labeled for SgIII and CgB in their periphery. Moreover, positive signals for SgIII were occasionally found in cells containing both prolactin and TSH in secretory granules. These lines of evidence suggest that SgIII and CgB are closely associated with the secretory granule membrane and that this membrane association might contribute to gathering and anchoring of other soluble constituents to the secretory granule membrane.

    DOI: 10.1177/002215540305100211

    Scopus

    PubMed

    researchmap

  • Expression of membrane-bound and soluble guanylyl cyclase mRNAs in embryonic and adult retina of the medaka fish Oryzias latipes 査読

    Tatsuo Harumi, Tsuyoshi Watanabe, Takehiro Yamamoto, Yasunori Tanabe, Norio Suzuki

    Zoological Science   20 ( 2 )   133 - 140   2003年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Localization of mRNAs for four membrane-bound guanylyl cyclases (membrane GCs
    OIGC3, OIGC4, OIGC5, and OIGC-R2), three soluble guanylyl cyclase subunits (soluble GC
    OIGCS-α1, OIGCS-α2, and OIGCS-β1), neuronal nitric oxide synthase (nNOS), and cGMP-dependent protein kinase I (cGK I) was examined in the embryonic and adult retinas of the medaka fish Oryzias latipes by in situ hybridization. All of the membrane GC mRNAs were detected in the photoreceptor cells of the adult and embryonic retinas, but in different parts
    the OIGC3 and OIGC5 mRNAs were expressed in the proximal part and the OIGC4 and OIGC-R2 mRNAs were expressed in the outer nuclear layer. The mRNA for nNOS was expressed in a scattered fashion on the inner side of the inner nuclear layer in the adult and embryonic retinas. The mRNAs (OIGCS-α2 and OIGCS-β1) of two soluble GC subunits (α2 and β1) were expressed mainly in the inner nuclear layer and the ganglion cell layer of the embryonic retina while the mRNAs of the soluble GC α1 subunit and cGK I were not detected in either the adult or embryonic retina. These results suggest that NO itself and/or the cGMP generated by soluble GC (α2/β1 heterodimer) play a novel role in the neuronal signaling and neuronal development in the medaka fish embryonic retina in addition to the role played by phototransduction through membrane GCs in the adult and embryonic retinas.

    DOI: 10.2108/zsj.20.133

    Scopus

    PubMed

    researchmap

  • Localization of the nitric oxide/cGMP signaling pathway-related genes and influences of morpholino knock-down of soluble guanylyl cyclase on medaka fish embryogenesis 査読

    T Yamamoto, Y Yao, T Harumi, N Suzuki

    ZOOLOGICAL SCIENCE   20 ( 2 )   181 - 191   2003年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    To better understand the nitric oxide (NO) / cyclic GMP (cGMP) signaling pathway during embryogenesis, we examined the spatial and temporal expression pattern of the genes for neuronal nitric oxide synthase (nNOS), soluble guanylyl cyclase (soluble GC) subunit (OIGCS-alpha(1), OIGCS-alpha(2), and OIGCS-beta(1)), and cGMP-dependent protein kinase (cGK) I and II (cGK I and cGK II) in the medaka fish embryos. OIGCS-beta(1) and nNOS were expressed maternally and OIGCS-alpha(1), OIGCS-alpha(2), cGK I, and cGK II were expressed zygotically. The zygotic expression of OIGCS-alpha(1) and cGK / was detected at stage 19, while that of OIGCS-a2 was detected at stage 16. Whole-mount in situ hybridization showed that the expression of nNOS or cGK / was localized in tail bud, otic vesicles, thyroid, and brain ventricle, or in thymus, gill arch, and olfactory pits, respectively, and that of OIGCS-alpha(1), OIGCS-alpha(2), or OIGCS-beta(1) was dim and dispersed throughout the embryos. To clarify the "role of the NO/cGMP signaling pathway in embryogenesis, we examined the influences of morpholino antisense oligonucleotide of the soluble GC subunit gene (alpha(1)-MO, alpha(2)-MO or beta(1)-MO) on development of medaka fish embryos. Embryos injected with alpha(1)-MO or alpha(2)-MO mainly exhibited abnormalities in the central nervous system, including defects in the formation of forebrain, eye, and otic vesicles. alpha(2)-MO injection caused cell death at the tail bud of the embryos at stage 22, and beta(1)-MO injection inhibited the development of the embryos at late blastula. These results suggest that the NO/cGMP signaling pathway plays critical roles in early embryogenesis.

    Web of Science

    researchmap

  • Separation and assay methods for melatonin and its precursors

    T Harumi, S Matsushima

    JOURNAL OF CHROMATOGRAPHY B   747 ( 1-2 )   95 - 110   2000年9月

     詳細を見る

    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Melatonin is an indoleamine hormone that is synthesized from tryptophan via 5-hydroxytryptophan, serotonin and N-acetylserotonin in the vertebrate pineal gland. Many chromatographic and non-chromatographic techniques have been developed and improved for the determination and measurement of melatonin and its related indoleamines. At present, gas chromatography with mass spectrometry and reversed-phase high-performance liquid chromatography with fluorescence or electrochemical detection are widely used for indoleamine determinations in the pineal gland. This review will deal with methods for the separation and determination of the melatonin and its related indoleamines. (C) 2000 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-4347(00)00064-5

    Web of Science

    researchmap

▼全件表示

書籍等出版物

  • 「生命と環境」 (第3章 細胞、第6章 受精と発生、第7章 脳と進化) 矢沢洋一、他編

    春見 達郎( 担当: 単著)

    三共出版  2010年4月 

     詳細を見る

    記述言語:日本語   著書種別:学術書

  • Effects of cations on sea urchin sperm metabolisms activated by sperm-activating peptide I

    Balkema/Rotterdam  1991年 

     詳細を見る

MISC

  • 培養ニューロンの分化に及ぼす神経接着分子IgSF11の効果

    林要喜知, 上田翼, 宮本泰則, 春見達郎, 鈴伸也

    日本動物学会大会予稿集   82nd   106   2011年8月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Measurement of melatonin in body fluids: Standards, protocols and procedures

    Eduardo Alves De Almeida, Paolo Di Mascio, Tatsuo Harumi, D. Warren Spence, Adam Moscovitch, Rüdiger Hardeland, Daniel P. Cardinali, Gregory M. Brown, S. R. Pandi-Perumal

    Child's Nervous System   27 ( 6 )   879 - 891   2011年6月

     詳細を見る

    記述言語:英語   掲載種別:書評論文,書評,文献紹介等  

    Background and Purpose: The circadian rhythm of melatonin in saliva or plasma, or of the melatonin metabolite 6-sulfatoxymelatonin (a6MTs) in urine, is a defining feature of suprachiasmatic nucleus (SCN) function, the body's endogenous oscillatory pacemaker. The primary objective of this review is to ascertain the clinical benefits and limitations of current methodologies employed for detection and quantification of melatonin in biological fluids and tissues. Data Identification: A search of the English-language literature (Medline) and a systematic review of published articles were carried out. Study Selection: Articles that specified both the methodology for quantifying melatonin and indicated the clinical purpose were chosen for inclusion in the review. Data Extraction: The authors critically evaluated the methodological issues associated with various tools and techniques (e.g. standards, protocols, and procedures). Results of Data Synthesis: Melatonin measurements are useful for evaluating problems related to the onset or offset of sleep and for assessing phase delays or advances of rhythms in entrained individuals. They have also become an important tool for psychiatric diagnosis, their use being recommended for phase typing in patients suffering from sleep and mood disorders. Additionally, there has been a continuous interest in the use of melatonin as a marker for neoplasms of the pineal region. Melatonin decreases such as found with aging are or post pinealectomy can cause alterations in the sleep/wake cycle. The development of sensitive and selective methods for the precise detection of melatonin in tissues and fluids has increasingly been shown to have direct relevance for clinical decision making. Conclusions: Due to melatonin's low concentration, as well as the coexistence of numerous other compounds in the blood, the routine determination of melatonin has been an analytical challenge. The available evidence indicates however that these challenges can be overcome and consequently that evaluation of melatonin's presence and activity can be an accessible and useful tool for clinical diagnosis. © Springer-Verlag 2010.

    DOI: 10.1007/s00381-010-1278-8

    Scopus

    PubMed

    researchmap

  • 神軽系における免疫グロブリンIgSF11の発現と局在

    林要喜知, 宮本泰則, 春見達郎, 鈴伸也

    日本動物学会大会予稿集   81st   143   2010年8月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Molecular cloning of a novel immunoglobulin superfamily gene preferentially expressed by brain and testis

    S Suzu, Y Hayashi, T Harumi, K Nomaguchi, M Yamada, H Hayasawa, K Motoyoshi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   296 ( 5 )   1215 - 1221   2002年9月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    We have cloned and characterized a novel gene from both human and mouse that encodes a new member of the immunoglobulin superfamily. The gene is preferentially expressed in both brain and testis, and hence, termed BT-IgSF (brain- and testis-specific immunoglobulin superfamily). The predicted protein consists of V-type and C2-type immunoglobulin domains as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. Human BT-IgSF protein (431 amino acids) is 88% identical to the mouse protein (428 amino acids) and both show significant homology to coxsackie and adenovirus receptor (CAR) and endothelial cell-selective adhesion molecule (ESAM). We examined the expression of BT-IgSF with various cultured cells and found that the gene was expressed in both neurons and glial cells in vitro. Furthermore, the expression was preferentially detected in pyramidal cell layers of the dentate gyrus and hippocampus and in commissure fibers of the corpus callosum, in brain tissue sections examined. These findings suggest that BT-IgSF plays a role in the development or function of the central nervous system. (C) 2002 Elsevier Science (USA). All rights reserved.

    DOI: 10.1016/S0006-291X(02)02025-9

    Web of Science

    researchmap

  • Molecular cloning of a novel immunoglobulin superfamily gene preferentially expressed by brain and testis

    S Suzu, Y Hayashi, T Harumi, K Nomaguchi, M Yamada, H Hayasawa, K Motoyoshi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   296 ( 5 )   1215 - 1221   2002年9月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    We have cloned and characterized a novel gene from both human and mouse that encodes a new member of the immunoglobulin superfamily. The gene is preferentially expressed in both brain and testis, and hence, termed BT-IgSF (brain- and testis-specific immunoglobulin superfamily). The predicted protein consists of V-type and C2-type immunoglobulin domains as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. Human BT-IgSF protein (431 amino acids) is 88% identical to the mouse protein (428 amino acids) and both show significant homology to coxsackie and adenovirus receptor (CAR) and endothelial cell-selective adhesion molecule (ESAM). We examined the expression of BT-IgSF with various cultured cells and found that the gene was expressed in both neurons and glial cells in vitro. Furthermore, the expression was preferentially detected in pyramidal cell layers of the dentate gyrus and hippocampus and in commissure fibers of the corpus callosum, in brain tissue sections examined. These findings suggest that BT-IgSF plays a role in the development or function of the central nervous system. (C) 2002 Elsevier Science (USA). All rights reserved.

    DOI: 10.1016/S0006-291X(02)02025-9

    Web of Science

    researchmap

  • Indoleamine metabolism in the pineal gland of the Chinese hamster, Cricetulus griseus

    T Harumi, S Matsushima

    GENERAL AND COMPARATIVE ENDOCRINOLOGY   109 ( 1 )   133 - 139   1998年1月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC  

    Day- and nighttime contents of pineal melatonin and other indoleamines were measured in the Chinese hamster, Cricetulus griseus. The hamsters were inbred in our colony and kept in LD 14:10. Absolute levels of pineal melatonin and N-acetylserotonin contents were extremely low, but showed a significant circadian rhythm with a minor peak during the light period. Pineal hydroxytryptophan and serotonin (5HT) contents were also found to be higher in the light period. The activity of N-acetyltransferase in the pineal was higher in the light period than in the dark period, whereas there was no difference in hydroxyindole-O-methyltransferase activity between the light and dark periods. The uptake level of tryptophan into the pineal gland was higher in the light period than in the dark period. These results suggest that the 5HT synthesis pathway and/or tryptophan uptake may be involved in forming the circadian rhythm of 5HT content in the Chinese hamster pineal gland. (C) 1998 Academic Press.

    DOI: 10.1006/gcen.1997.7014

    Web of Science

    researchmap

  • Chromosome localization of the mouse zonadhesin gene and the human zonadhesin gene (ZAN)

    Z Gao, T Harumi, DL Garbers

    GENOMICS   41 ( 1 )   119 - 122   1997年4月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Zonadhesin is a recently discovered sperm membrane protein that binds to the extracellular matrix of the egg in a species-specific manner. The gene for zonadhesin maps to chromosome 5 in the mouse (Zan) and to chromosome 7 in the human (ZAN) in a position near the gene for the egg extracellular matrix protein ZP3. (C) 1997 Academic Press.

    DOI: 10.1006/geno.1997.4620

    Web of Science

    PubMed

    researchmap

  • Characterization of sea urchin sperm membrane proteins which interact with a major acrosome reaction-inducing substance, fucose sulfate glycoconjugate

    Y Satoh, T Shimizu, T Harumi, N Suzuki

    ZOOLOGICAL SCIENCE   13 ( 3 )   377 - 383   1996年6月

     詳細を見る

    記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Intact sea urchin spermatozoa were successfully biotinylated with NHS-LC-Biotin and the biotinylated spermatozoa retained the viability. Analysis of the membrane prepared from the biotinylated spermatozoa of the sea urchin Hemicentrotus pulcherrimus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that several proteins such as wheat-germ agglutinin (WGA)-binding protein (220 kDa), guanylyl cyclase (131 kDa), sperm-activating peptide-I (SAP-I)-crosslinking protein (71 kDa), GPI-anchored protein (63 kDa) and functionally unknown proteins (50 kDa and 30 kDa) were specifically biotinylated. Experiments using spermatozoa of sea urchins, Anthocidaris crassispina and Clypeasfer japonicus showed that several proteins similar to those of H. pulcherrimus spermatozoa were also labeled with NHS-LC-Biotin.
    Fucose sulfate glycoconjugate (FSG) isolated from the jelly coat of H. pulcherrimus was mixed with solubilized biotinylated sperm membrane proteins of H. pulcherrimus, A. crassispina or C. japonicus and then subjected to gel filtration chromatography on a Sepharose 2B column, indicating that only two biotinylated H. pulcherrimus sperm proteins were coeluted with H. pulcherrimus FSG.

    Web of Science

    researchmap

  • Simultaneous determination of serotonin, N-acetylserotonin and melatonin in the pineal gland of the juvenile golden hamster by high-performance liquid chromatography with electrochemical detection

    T Harumi, H Akutsu, S Matsushima

    JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS   675 ( 1 )   152 - 156   1996年1月

     詳細を見る

    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    A simple and simultaneous determination of melatonin and its precursors, serotonin (5-HT) and N-acetylserotonin, was achieved by reversed-phase high-performance liquid chromatography with electrochemical detection. The addition of an ion-paring agent, sodium 1-octanesulfonate, to the chromatographic mobile phase caused an increase of the retention time of 5-HT, and resulted in the successful simultaneous resolution of these three indoleamines. This method was used to quantitate these indoleamines in the pineal gland of juvenile golden hamsters.

    DOI: 10.1016/0378-4347(95)00345-2

    Web of Science

    PubMed

    researchmap

  • Sea urchin sperm receptors for egg peptides

    HARDY D. M.

    Dev. Biol.   5 ( 4 )   217 - 224   1994年

  • In vitro Autophosphorylation and Cyclic Nucleotide-Dependent Dephosphorylation of Sea Urchin Sperm Histone Kinase :

    Harumi Tatsuo, Hoshino Katsuaki, Suzuki Norio

    Zoological science   11 ( 2 )   209 - 219   1994年

     詳細を見る

    記述言語:英語   出版者・発行元:Zoological Society of Japan  

    We identified two phosphorylatable proteins (33 kDa and 48 kDa) in crude extractes of spermatozoa from the sea urchin, Hemicentrotus pulcherrimus. While the 33 kDa protein was phosphorylated in 100 mM Mg^<2+>-containing medium with cAMP or cGMP, phosphorylation of the 48 kDa protein occurred irrespective of the Mg^<2+>-concentration if it was more than 10 mM. Physiological concentration if it was more than 10 mM. Physiological concentrations of cAMP or cGMP triggered dephosphorylation of the [^<32>P]-phosphorylated 48 kDa protein in a concentration dependent manner. The dephosphorylation of 48 kDa protein was inhibited with calyculin A or okadaic acid. The 48 kDa protein was associated with a 39 kDa protein to form a larger oligomer with about 400 kDa. The purified 400 kDa protein showed cyclic nucleotide-dependent histone kinase activity. The histone kinase activity shifted from 400 kDa to 39 kDa on a Superose 6 HR in the presence of 5×10^<-6>M cAMP. Phosphorylation of the 48 kDa protein in the purified 400 kDa protein required only Mg[^<32>P]ATP. A photoaffinity cAMP analogue, 8-N_3-[^<32>P]cAMP, was incorporated into the 48 kDa protein. These results suggest that the 48 kDa protein which is a reguratory subunit of the histone kinase is autophosphorylated, and binding of cAMP to the phosphorylated 48 kDa subunit of the kinase results in dissociation of the 48 kDa subunit(s) from the 39 kDa subunit(s) to allow the 48 kDa subunit accessible to a protein phosphatase.

    CiNii Books

    researchmap

    その他リンク: http://dl.ndl.go.jp/info:ndljp/pid/10860784

  • DIFFERENTIAL-EFFECTS OF THE EGG JELLY MOLECULES FSG AND SAP-I ON ELEVATION OF INTRACELLULAR CA-2+ AND PH IN SEA-URCHIN SPERMATOZOA

    K HOSHINO, T SHIMIZU, Y SENDAI, T HARUMI, N SUZUKI

    DEVELOPMENT GROWTH & DIFFERENTIATION   34 ( 4 )   403 - 411   1992年8月

     詳細を見る

    記述言語:英語   出版者・発行元:BLACKWELL SCIENCE  

    DOI: 10.1111/j.1440-169X.1992.00403.x

    Web of Science

    researchmap

  • PURIFICATION AND CHARACTERIZATION OF SPERM CREATINE-KINASE AND GUANYLATE-CYCLASE OF THE SEA-URCHIN HEMICENTROTUS-PULCHERRIMUS

    T HARUMI, M KURITA, N SUZUKI

    DEVELOPMENT GROWTH & DIFFERENTIATION   34 ( 2 )   151 - 162   1992年4月

     詳細を見る

    記述言語:英語   出版者・発行元:BLACKWELL SCIENCE  

    DOI: 10.1111/j.1440-169X.1992.tb00004.x

    Web of Science

    researchmap

  • CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN DEPHOSPHORYLATION IN SPERMATOZOA OF SEA URCHIN HEMICENTROTUS PULCHERRIMUS(Developmental Biology)Proceedings of the Sixty-Third Annual Meeting of the Zoologiacal Socistry of Japan :

    Harumi T., Hoshino K., Suzuki N.

    Zoological science   9 ( 6 )   1204 - 1204   1992年

     詳細を見る

    記述言語:英語   出版者・発行元:Zoological Society of Japan  

    CiNii Books

    researchmap

    その他リンク: http://id.nii.ac.jp/1141/00031842/

  • Effects of Sperm-Activating Peptide I on ┣DBHemicentrotus pulcherrimus(/)-┫DB Spermatozoa in High Potassium Sea Water

    Development, Growth and Differentiation   34 ( 2 )   163 - 172   1992年

  • LOCALIZATION AND CHARACTERIZATION OF PHOSPHATIDYLCHOLINE IN SEA-URCHIN SPERMATOZOA

    M MITA, T HARUMI, N SUZUKI, N UETA

    JOURNAL OF BIOCHEMISTRY   109 ( 2 )   238 - 242   1991年2月

     詳細を見る

    記述言語:英語   出版者・発行元:JAPAN BIOCHEMICAL SOC  

    Sea urchin spermatozoa obtain energy for movement through oxidation of endogenous phospholipids, particularly phosphatidylcholine (PC). This study was undertaken to determine the localization of PC available for utilization in energy metabolism in spermatozoa of the sea urchin, Hemicentrotus pulcherrimus. Following incubation with sea water, the PC content in sperm heads decreased significantly, while that in sperm tails did not change. PC was abundant in sperm heads, particularly the midpieces. PC composed of unsaturated fatty acids was consumed to a greater extent during incubation than that consisting of saturated fatty acids. Analysis by gas-liquid chromatography indicated most of fatty acid moieties in the midpieces PC to be unsaturated. Phospholipase A2 activity was also distributed in sperm heads, particularly the midpieces. It thus appears that PC as a substrate for energy metabolism is located in the midpieces of sea urchin spermatozoa.

    Web of Science

    researchmap

  • Receptors for Sperm‐activating Peptides, SAP‐I and SAP‐IIB, on Spermatozoa of Sea Urchins, Hemicentrotus pulcherrimus and Glyptocidaris crenularis Sea urchin spermatozoa/Sperm‐activating peptides/Receptor/Cross‐link

    Tatsuo Harumi, Masaaki Yamaguchi, Norio Suzuki

    Development, Growth &amp; Differentiation   33 ( 1 )   67 - 73   1991年

     詳細を見る

    記述言語:英語  

    Sperm‐activating peptide analogues, GYGG‐SAP‐I (Gly‐Tyr‐Gly‐Gly‐Gly‐Phe‐Asp‐Leu‐Asn‐Gly‐Gly‐Gly‐Val‐Gly) and GYGG‐SAP‐IIB (Gly‐Tyr‐Gly‐Gly‐Lys‐Leu‐Cys‐Pro‐Gly‐Gly‐Asn‐Cys‐Val) which exibit almost the same respiration‐stimulating activity as the respective original peptide were chemically synthesized, radiolabeled with Na1251, and used for experiments of binding and cross‐linking to Hemicentrotus pulcherrimus or Glyptocidaris crenularis spermatozoa. In competitive binding, SAP‐I caused 50% decrease in 125l‐GYGG‐SAP‐l binding to intact spermatozoa, sperm heads and sperm tails of H. pulcherrimus at the concentrations of 282 nM, 3 nM and 141 nM, respectively. The incubations of H. pulcherrimus sperm tails with 125l‐GYGG‐SAP‐I and the chemical cross‐linking reagent, disuccinimidyl suberate, resulted in the appearance of two radiolabeled bands with apparent molecular masses of 71 kDa and 63 kDa (determined by sodium dodecyl sulfate‐gel electrophoresis under reducing conditions). 125l‐GYGG‐SAP‐IIB bound almost equally to sperm heads and sperm tails of G. crenularis, and was cross‐linked to 172 kDa, 62 kDa and 58 kDa proteins in sperm heads, and 157 kDa and 62 kDa proteins in sperm tails with disuccinimidyl suberate. Copyright © 1991, Wiley Blackwell. All rights reserved

    DOI: 10.1111/j.1440-169X.1991.00067.x

    Scopus

    researchmap

  • THE CRAB-EATING FROG RANA-CANCRIVORA - SERUM MINERAL CONCENTRATIONS AND HISTOLOGY OF THE ULTIMOBRANCHIAL GLAND AND THE PARATHYROID-GLAND

    Y SASAYAMA, C OGURO, T OGASAWARA, T HIRANO, T HARUMI, S WISESSANG

    GENERAL AND COMPARATIVE ENDOCRINOLOGY   80 ( 3 )   399 - 406   1990年12月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Web of Science

    researchmap

  • THE INFLUENCE OF AN EGG-ASSOCIATED PEPTIDE ON ENERGY-METABOLISM IN SEA-URCHIN SPERMATOZOA - THE PEPTIDE STIMULATES PREFERENTIAL HYDROLYSIS OF PHOSPHATIDYLCHOLINE AND OXIDATION OF FATTY-ACID

    M MITA, N UETA, T HARUMI, N SUZUKI

    BIOCHIMICA ET BIOPHYSICA ACTA   1035 ( 2 )   175 - 181   1990年8月

     詳細を見る

    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Web of Science

    researchmap

  • PHOSPHOLIPID UTILIZATION IN ASCIDIAN CIONA-INTESTINALIS SPERMATOZOA DURING SWIMMING

    T HARUMI, R DESANTIS, MR PINTO, N SUZUKI

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY   96 ( 2 )   263 - 265   1990年

     詳細を見る

    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Web of Science

    researchmap

  • PHOSPHATIDYLCHOLINE IN SEA-URCHIN SPERMATOZOA, AND ITS ROLE IN ENERGY-METABOLISM

    M MITA, T HARUMI, N SUZUKI, N UETA

    DEVELOPMENT GROWTH & DIFFERENTIATION   30 ( 4 )   410 - 410   1988年8月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:BLACKWELL SCIENCE  

    Web of Science

    researchmap

▼全件表示

講演・口頭発表等

  • 魚類の受精における卵門の精子に対する誘引機構

    春見 達郎,松原 創,小林寛、甲賀大輔、柳町 隆造

    ㈳日本動物学会 第94回山形大会   ㈳日本動物学会

     詳細を見る

    開催年月日: 2023年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:山形  

  • トウガレイの初期発生を制御する環境要因

    小林 寛 他

    第6回富山湾研究会 

     詳細を見る

    開催年月日: 2023年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

  • Gonadal sexual plasticity in tiger puffer Takifugu rubripes 国際会議

    Nakade,M, Rafiuddin,M.A, Kobayashi,H, Kobayshi,S, Sakai,K, Inada.K, Shigematsu,K, Nagami,A, Ogiso,s, Toyota,K, Tabuchi,Y, Furusawa,Y, Hirayama,J, Harumi, T, Suzuki,N, Matsubara,H

    国際シンポジウム「越境汚染と社会環境への影響」  金沢大学環日本海域環境研究センター

     詳細を見る

    開催年月日: 2022年12月

    記述言語:英語   会議種別:ポスター発表  

    開催地:online  

  • Effect of environmental factors on the embryonic development of the rosy seabass Doederleinia berycoides 国際会議

    Rafiuddin,M.A, Sakai,K, Kobayashi, S, Shigematsu,K, Nakade,M, Inada.K, Kobayshi,H, Nagami,A, Ogiso,S, Toyota,K, Tabuchi,Y, Furusawa,Y, Hirayama,J, Harumi, T, Suzuki,N, Matsubara,H

    国際シンポジウム「越境汚染と社会環境への影響」  金沢大学環日本海域環境研究センター

     詳細を見る

    開催年月日: 2022年12月

    記述言語:英語   会議種別:ポスター発表  

    開催地:online  

  • Reproductive behavior of mature tiger puffer Takifugu rubripes in Noto, Japan 国際会議

    Kobayashi,S, Rafiuddin,M.A, Nakade,M, Kobayshi,H, Sakai,K, Inada.K, Shigematsu,K, Nagami,A, Ogiso,S, Toyota,K, Tabuchi,Y, Furusawa,Y, Hirayama,J, Harumi, T, Suzuki,N, Matsubara,H

    国際シンポジウム「越境汚染と社会環境への影響」  金沢大学環日本海域環境研究センター

     詳細を見る

    開催年月日: 2022年12月

    記述言語:英語   会議種別:ポスター発表  

    開催地:online  

  • 温故知新~受精後のメダカ卵の Ca 上昇~

    春見 達郎,毛利 達磨, 成瀬 清,松原 創,柳町 隆造

    ㈳日本動物学会 第-93回早稲田大会   ㈳日本動物学会

     詳細を見る

    開催年月日: 2022年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

  • 日本海産魚類の受精:精子の卵門進入の解析

    春見達郎・松原創・鈴木信雄・柳町隆造

    金沢大学環日本海環境研究センター2021年度共同研究成果報告会  金沢大学環日本海環境研究センター

     詳細を見る

    開催年月日: 2022年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

  • Mechanisms for Scavenging Reactive Oxygen Species in Mammalian and Fish Spermatozo 国際会議

    Tatsuo Harumi, Naomi Shishido, Masao Nakamura, Yokichi Hayashi, Norihiko Shimizu, Takashi Ichikawa, Tadashi Andoh, Ryuzo Yanagimachi

    50th Annual Meeting of American Society of Cell Biology 

     詳細を見る

    開催年月日: 2010年12月

    記述言語:英語   会議種別:ポスター発表  

    開催地:Philadelphia, USA  

  • Diversity of Motility and Energy Metabolism in Fish Spermatozoa 国際会議

    Tatsuo Harumi, Tadashi Andoh, Takahiro Matsubara, Norihiko Shimizu, Toshiyuki Hayakawa, Yokichi Hayashi, Ryuzo Yanagimachi

    41th Annual Meeting of the Society for the Study of Reproduction 

     詳細を見る

    開催年月日: 2008年5月

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kona, USA  

▼全件表示

受賞

  • Zoological Science Award 

    2004年4月   日本動物学会  

    春見達郎

     詳細を見る

    受賞区分:国内学会・会議・シンポジウム等の賞  受賞国:日本国

共同研究・競争的資金等の研究課題

  • 精子形成における細胞接着分子の機能解析

      詳細を見る

    資金種別:競争的資金

    researchmap

  • cGMP、グアニル酸シクラーゼ

      詳細を見る

    資金種別:競争的資金

    researchmap

  • cGMP signaling mechanisms in cell activation

      詳細を見る

    資金種別:競争的資金

    researchmap

職務上の実績に関する事項

  • 2024年4月 -2025年3月
    旭川ウェルビーイング・コンソーシアム事業に対する補助金の獲得

学術貢献活動

  • (社)日本動物学会 北海道支部第56回大会 実行委員長

    2010年8月