Updated on 2025/06/29

写真a

 
HINO Toshiaki
 
Organization
School of Medicine General Education Biology
External link

Degree

  • 獣医学 ( 2011.10   北里大学 )

Research Interests

  • Developmental engineering

  • 生殖医学

  • 実験動物

  • 生殖生物学

  • Reproductive biology

  • Reproductive medicine

Research Areas

  • Life Science / Obstetrics and gynecology  / 生殖医学

  • Life Science / Laboratory animal science  / 実験動物

Education

  • Kitasato University   Graduate School of Veterinary Medicine and Animal Sciences

    2010.4 - 2011.9

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  • Kitasato University

    1995.4 - 2001.3

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Research History

  • 旭川医科大学 医学部   医学部 生物学教室   准教授

    2024.5

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  • Asahikawa Medical University   School of Medicine

    2014.4

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  • Asahikawa Medical College

    2014.4 - 2024.4

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  • Asahikawa Medical University   School of Medicine   Assistant Professor

    2009.4 - 2014.3

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  • 株式会社 三菱化学生命科学研究所   特別技術員

    2001.4 - 2009.3

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  • 三菱化学生命科学研究所   生殖工学開発室   特別技術員

    2001.4 - 2009.3

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Professional Memberships

  • アメリカ生殖学会（Society for the Study of Reproduction）

    2019.1

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  • 財団法人染色体学会

    2011.4

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  • 日本哺乳動物卵子学会

    2003.4

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Committee Memberships

  • 日本生殖医学会   学術委員会 委員  

    2020.12 - 2022.6   

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  • 動物生殖工学研究会   理事  

    2008.12   

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  •   動物生殖工学研究会  

    2006.4   

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  •   日本生殖医学会  

    2005.4   

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Papers

  • Oocyte transport against fluid flow to the fertilization site in mice: contributions of cilia beating and peristalsis†. International journal

    Toshiaki Hino

    Biology of reproduction   2025.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    The transport of ovulated oocytes to the site of fertilization involves two main processes, the initial collection of the oocyte by the fimbria and its subsequent transport through the upper ampulla. These are crucial events preceding fertilization. Around ovulation, the oviduct exhibits active fluid secretion, peristaltic movements, and ciliary beating, all of which are believed to be involved in oocyte transport. However, their specific contributions require further clarification. In this study, we investigated how these three factors influence oocyte transport to the fertilization site in the oviduct in vivo. The oviduct of anesthetized mice was installed in a fluid-circulating chamber. By introducing fixed and stained cumulus-oocyte complexes (COCs) into the fimbria and injecting a small amount of ink into the oviduct lumen, we monitored oocyte transport and fluid dynamics. Interestingly, while oviduct fluid flowed toward the fimbria, the COC moved in the opposite direction to reach the site of fertilization. Inhibiting ciliary beating disrupted both the collection (or "pickup") and transport of the oocyte, whereas inhibiting peristalsis had no immediate impact on these processes. However, extended inhibition of peristalsis resulted in impaired oocyte transport. Under these conditions, fluid accumulated, and the oviduct lumen expanded, disrupting the intimate contact between the COC and the cilia. These findings indicate that ciliary beating, rather than fluid flow or peristalsis, propels the COC against the fluid flow toward the fertilization site. In addition, peristalsis maintains the luminal conditions required for effective transmission of ciliary propulsion to the COC.

    DOI： 10.1093/biolre/ioaf139

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  • Characterization of H3K4me3 in mouse oocytes at the metaphase II stage. International journal

    Atsushi Takasu, Toshiaki Hino, Osamu Takenouchi, Yasuki Miyagawa, Zhihua Liang, Shota Tanaka, Tomoya Mimura, Chisato Ida, Yuki Matsuo, Yuna Lee, Haruka Ikegami, Miho Ohsugi, Shogo Matoba, Atsuo Ogura, Kazuo Yamagata, Kazuya Matsumoto, Tomoya S Kitajima, Kei Miyamoto

    The Journal of biological chemistry   110308 - 110308   2025.5

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    Central functions of histone modifications in germ cell and embryonic development have been documented. Accumulating evidence suggests that oocytes possess unique profiles of histone modifications, among which histone H3 lysine 4 trimethylation (H3K4me3) is broadly spread on the mouse oocyte chromosomes at the metaphase II (MII) stage, unlike later embryonic stages. However, the characteristics and developmental roles of H3K4me3 on MII chromosomes are unclear. Here, we discovered that H3K4me3 was abundantly localized on some of the MII oocyte chromosomes facing the cortical side. Using multicolor FISH and CRISPR-Sirius-based labeling of chromosomes, we revealed that the X chromosome tended to be localized at the cortical side with strong H3K4me3 signals. Anchoring oocyte chromosomes to the cortex may play a role in the asymmetric H3K4me3 distribution. Furthermore, we found that the forced removal of H3K4me3 through the overexpression of a specific lysine demethylase in MII oocytes resulted in abnormal chromosome-spindle structure and impaired preimplantation development after in vitro fertilization. These findings highlight the developmental function of H3K4me3 in transcriptionally-silent MII oocytes.

    DOI： 10.1016/j.jbc.2025.110308

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  • A reliable technique for karyotyping mouse oocytes prepared by a gradual fixation/air-drying method followed by multicolour FISH. International journal

    Toshiaki Hino, Hirokazu Kusakabe

    Biology open   12 ( 12 )   2023.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    Chromosome segregation errors during oocyte meiosis increase with age and lead to aneuploidy; hence, the mechanism has been studied extensively. The mouse is the most widely used experimental animal for this purpose. However, the lack of a reliable and efficient technique for karyotyping mouse oocytes has limited comprehensive studies of chromosome-specific segregation errors in this animal model. Here, we developed a novel karyotyping technique for mouse oocytes by applying multicolour fluorescence in situ hybridisation (FISH) to chromosome slides prepared by a gradual fixation/air-drying method, which is best suited to avoid rupture of oocyte membrane and artificial loss of chromosomes. The success rate of karyotyping meiosis I and II oocytes was about 30%, which improved to over 90% when the oocytes were 'flattened' during fixation and the chromosome specimens were denatured at 4°C. When this technique was applied to the karyotyping of meiosis II oocytes from aged female mice and from young female mice injected with colchicine, more than 80% of the oocytes were successfully karyotyped and the number of chromosomes was identified on all aberrant chromosomes. In conclusion, our technique allows for the efficient and reliable karyotyping of mouse oocytes.

    DOI： 10.1242/bio.060188

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  • Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice. International journal

    Seiya Oura, Toshiaki Hino, Takashi Satoh, Taichi Noda, Takayuki Koyano, Ayako Isotani, Makoto Matsuyama, Shizuo Akira, Kei-Ichiro Ishiguro, Masahito Ikawa

    PLoS genetics   18 ( 6 )   e1010241   2022.6

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    Meiosis is a hallmark event in germ cell development that accompanies sequential events executed by numerous molecules. Therefore, characterization of these factors is one of the best strategies to clarify the mechanism of meiosis. Here, we report tripartite motif-containing 41 (TRIM41), a ubiquitin ligase E3, as an essential factor for proper meiotic progression and fertility in male mice. Trim41 knockout (KO) spermatocytes exhibited synaptonemal complex protein 3 (SYCP3) overloading, especially on the X chromosome. Furthermore, mutant mice lacking the RING domain of TRIM41, required for the ubiquitin ligase E3 activity, phenocopied Trim41 KO mice. We then examined the behavior of mutant TRIM41 (ΔRING-TRIM41) and found that ΔRING-TRIM41 accumulated on the chromosome axes with overloaded SYCP3. This result suggested that TRIM41 exerts its function on the chromosome axes. Our study revealed that Trim41 is essential for preventing SYCP3 overloading, suggesting a TRIM41-mediated mechanism for regulating chromosome axis protein dynamics during male meiotic progression.

    DOI： 10.1371/journal.pgen.1010241

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  • Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes. International journal

    Narumi Ogonuki, Hirohisa Kyogoku, Toshiaki Hino, Yuki Osawa, Yasuhiro Fujiwara, Kimiko Inoue, Tetsuo Kunieda, Seiya Mizuno, Hiroyuki Tateno, Fumihiro Sugiyama, Tomoya S Kitajima, Atsuo Ogura

    EMBO reports   e54992   2022.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    Microinjection of spermatozoa or spermatids into oocytes is a major choice for infertility treatment. However, the use of premeiotic spermatocytes has never been considered because of its technical problems. Here, we show that the efficiency of spermatocyte injection in mice can be improved greatly by reducing the size of the recipient oocytes. Live imaging showed that the underlying mechanism involves reduced premature separation of the spermatocyte's meiotic chromosomes, which produced much greater (19% vs. 1%) birth rates in smaller oocytes. Application of this technique to spermatocyte arrest caused by STX2 deficiency, an azoospermia factor also found in humans, resulted in the production of live offspring. Thus, the microinjection of primary spermatocytes into oocytes may be a potential treatment for overcoming a form of nonobstructive azoospermia caused by meiotic failure.

    DOI： 10.15252/embr.202254992

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  • Active peristaltic movements and fluid production of the mouse oviduct: their roles in fluid and sperm transport and fertilization†. Reviewed

    Hino T, Yanagimachi R

    Biology of reproduction   101 ( 1 )   40 - 49   2019.7

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  • The Behavior and Acrosomal Status of Mouse Spermatozoa In Vitro, and Within the Oviduct During Fertilization after Natural Mating Reviewed

    Toshiaki Hino, Yuko Muro, Miwa Tamura-Nakano, Masaru Okabe, Hiroyuki Tateno, Ryuzo Yanagimachi

    BIOLOGY OF REPRODUCTION   95 ( 3 )   50   2016.9

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    DOI： 10.1095/biolreprod.116.140400

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  • Developmental potential of 2n/3n mixoploid mouse embryos produced by fusion of individual second polar bodies and blastomeres of 2-cell embryos Reviewed

    Toshiaki Hino, Hiroyuki Tateno

    REPRODUCTION FERTILITY AND DEVELOPMENT   28 ( 12 )   1982 - 1989   2016

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    DOI： 10.1071/RD15081

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  • Sirh7/Ldoc1 knockout mice exhibit placental P4 overproduction and delayed parturition Reviewed

    Mie Naruse, Ryuichi Ono, Masahito Irie, Kenji Nakamura, Tamio Furuse, Toshiaki Hino, Kanako Oda, Misho Kashimura, Ikuko Yamada, Shigeharu Wakana, Minesuke Yokoyama, Fumitoshi Ishino, Tomoko Kaneko-Ishino

    DEVELOPMENT   141 ( 24 )   4763 - 4771   2014.12

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    DOI： 10.1242/dev.114520

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  • Ca2+ ionophore A23187 can make mouse spermatozoa capable of fertilizing in vitro without activation of cAMP-dependent phosphorylation pathways Reviewed

    Hiroyuki Tateno, Dario Krapf, Toshiaki Hino, Claudia Sanchez-Cardenas, Alberto Darszon, Ryuzo Yanagimachi, Pablo E. Visconti

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   110 ( 46 )   18543 - 18548   2013.11

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    DOI： 10.1073/pnas.1317113110

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  • Chromosomal stability of second polar bodies in mouse embryos Reviewed

    Toshiaki Hino, Hirokazu Kusakabe, Hiroyuki Tateno

    JOURNAL OF ASSISTED REPRODUCTION AND GENETICS   30 ( 1 )   91 - 98   2013.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10815-012-9899-3

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  • Hippocampal function is not required for the precision of remote place memory Reviewed

    Takashi Kitamura, Reiko Okubo-Suzuki, Noriko Takashima, Akiko Murayama, Toshiaki Hino, Hirofumi Nishizono, Satoshi Kida, Kaoru Inokuchi

    MOLECULAR BRAIN   5   5   2012.2

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    DOI： 10.1186/1756-6606-5-5

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  • Accelerated modification of the zona pellucida is the primary cause of decreased fertilizability of oocytes in the 129 inbred mouse strain Reviewed

    Toshiaki Hino, Kanako Oda, Kenji Nakamura, Hiroyuki Tateno, Yutaka Toyoda, Minesuke Yokoyama

    ZYGOTE   19 ( 4 )   315 - 322   2011.11

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    DOI： 10.1017/S0967199410000481

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  • Coordinated regulation of differentiation and proliferation of embryonic cardiomyocytes by a jumonji (Jarid2)-cyclin D1 pathway Reviewed

    Kuniko Nakajima, Masayo Inagawa, Chiharu Uchida, Kumiko Okada, Shoji Tane, Mizuyo Kojima, Misae Kubota, Masatsugu Noda, Satoko Ogawa, Haruki Shirato, Michio Sato, Rika Suzuki-Migishima, Toshiaki Hino, Yukio Satoh, Masatoshi Kitagawa, Takashi Takeuchi

    DEVELOPMENT   138 ( 9 )   1771 - 1782   2011.5

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    DOI： 10.1242/dev.059295

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  • PolyADP-Ribosylation Is Required for Pronuclear Fusion during Postfertilization in Mice Reviewed

    Tomoharu Osada, Hideki Ogino, Toshiaki Hino, Sachiyo Ichinose, Kenji Nakamura, Akira Omori, Toshiaki Noce, Mitsuko Masutani

    PLOS ONE   5 ( 9 )   2010.9

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    DOI： 10.1371/journal.pone.0012526

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  • Activin plays a key role in the maintenance of long-term memory and late-LTP Reviewed

    Hiroshi Ageta, Shiro Ikegami, Masami Miura, Masao Masuda, Rika Migishima, Toshiaki Hino, Noriko Takashima, Akiko Murayama, Hiromu Sugino, Mitsutoshi Setou, Satoshi Kida, Minesuke Yokoyama, Yoshihisa Hasegawa, Kunihiro Tsuchida, Toshihiko Aosaki, Kaoru Inokuchi

    LEARNING & MEMORY   17 ( 4 )   176 - 185   2010.4

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    DOI： 10.1101/lm.16659010

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  • Validity of a simple vitrification technique for chromosome study of mouse one-cell embryos Reviewed

    Toshiaki Hino, Hirokazu Kusakabe, Hiroyuki Tateno

    Chromosome Science   13 ( 3 )   45 - 48   2010

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：THE SOCIETY OF CHROMOSOME RESEARCH  

    To evaluate cytogenetic validity of a simple vitrification technique for embryo cryopreservation, mouse 1-cell embryos were vitrified 4, 6, and 8 h after <i>in vitro</i> fertilization (IVF). In addition, chromosomal damage of spermatozoa treated with methyl methanesulfonate (MMS) was estimated using vitrified 1-cell embryos. More than 90% of embryos survived vitrification regardless of the time after IVF. In the 4-h and 6-h groups, some of the surviving embryos swelled after recovery. The incidence of structural chromosome aberrations and aneuploidy in embryos with morphologically normal features did not significantly increase in any group. The vitrification technique preserved 1-cell embryos derived from MMS-treated spermatozoa without alteration of chromosome damage. This technique will enable us to manage the optimal time for chromosome preparation of mouse 1-cell embryos.

    DOI： 10.11352/scr.13.45

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    Other Link： http://amcor.asahikawa-med.ac.jp/modules/xoonips/detail.php?id=CS13-3-45

  • Marked Improvement of Fertility of Cryopreserved C57BL/6J Mouse Sperm by Depletion of Ca2+ in Medium Reviewed

    Rika Suzuki-Migishima, Toshiaki Hino, Miho Takabe, Kanako Oda, Fujio Migishima, Yoshiharu Morimoto, Minesuke Yokoyama

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   55 ( 4 )   386 - 392   2009.8

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    DOI： 10.1262/jrd.20163

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  • Paternal deletion of Meg1/Grb10 DMR causes maternalization of the Meg1/Grb10 cluster in mouse proximal Chromosome 11 leading to severe pre- and postnatal growth retardation Reviewed

    Hirosuke Shiura, Kenji Nakamura, Takafusa Hikichi, Toshiaki Hino, Kanako Oda, Rika Suzuki-Migishima, Takashi Kohda, Tomoko Kaneko-Ishino, Fumitoshi Ishino

    HUMAN MOLECULAR GENETICS   18 ( 8 )   1424 - 1438   2009.4

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    DOI： 10.1093/hmg/ddp049

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  • Low fertility in vivo resulting from female factors causes small litter size in 129 inbred mice Reviewed

    Toshiaki Hino, Kanako Oda, Kenji Nakamura, Yutaka Toyoda, Minesuke Yokoyama

    Reproductive Medicine and Biology   8 ( 4 )   157 - 161   2009

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：John Wiley and Sons Ltd  

    DOI： 10.1007/s12522-009-0024-y

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  • Requirement of the immediate early gene vesl-1S/homer-1a for fear memory formation Reviewed

    Naoko Inoue, Harumi Nakao, Rika Migishima, Toshiaki Hino, Minoru Matsui, Fumihiko Hayashi, Kazuki Nakao, Toshiya Manabe, Atsu Aiba, Kaoru Inokuchi

    MOLECULAR BRAIN   2   7   2009

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/1756-6606-2-7

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  • Role of retrotransposon-derived imprinted gene, Rtl1, in the feto-maternal interface of mouse placenta Reviewed

    Yoichi Sekita, Hirotaka Wagatsuma, Kenji Nakamura, Ryuichi Ono, Masayo Kagami, Noriko Wakisaka, Toshiaki Hino, Rika Suzuki-Migishima, Takashi Kohda, Atsuo Ogura, Tsutomu Ogata, Minesuke Yokoyama, Tomoko Kaneko-Ishino, Fumitoshi Ishino

    NATURE GENETICS   40 ( 2 )   243 - 248   2008.2

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    DOI： 10.1038/ng.2007.51

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  • Motor discoordination of transgenic mice overexpressing a microtubule destabilizer, stathmin, specifically in Purkinje cells Reviewed

    Noriaki Ohkawa, Kouichi Hashimoto, Toshiaki Hino, Rika Migishima, Minesuke Yokoyama, Masanobu Kano, Kaoru Inokuchi

    NEUROSCIENCE RESEARCH   59 ( 1 )   93 - 100   2007.9

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    DOI： 10.1016/j.neures.2007.06.1464

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  • Cryoprotective effects of various saccharides on cryopreserved mouse sperm from various strains Reviewed

    Toshiaki Hino, Miho Takabe, Rika Suzuki-migishima, Minesuke Yokoyama

    Reproductive Medicine and Biology   6 ( 4 )   229 - 233   2007

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：John Wiley and Sons Ltd  

    DOI： 10.1111/j.1447-0578.2007.00190.x

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  • Deletion of Peg10, an imprinted gene acquired from a retrotransposon, causes early embryonic lethality Reviewed

    R Ono, K Nakamura, K Inoue, M Naruse, T Usami, N Wakisaka-Saito, T Hino, R Suzuki-Migishima, N Ogonuki, H Miki, T Kohda, A Ogura, M Yokoyama, T Kaneko-Ishino, F Ishino

    NATURE GENETICS   38 ( 1 )   101 - 106   2006.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/ng1699

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MISC

  • マウス生体の卵管における卵子輸送の観察~卵管液流・繊毛拍動・蠕動運動との関係に着目して~

    日野敏昭, 柳町隆造

    Journal of Mammalian Ova Research   41 ( 1 )   2024

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  • 顕微授精技術を用いた無精子症マウスからの産子作出

    越後貫成美, 日野敏昭, 大澤優生, 藤原靖浩, 水野聖哉, 井上貴美子, 井上貴美子, 国枝哲夫, 田崎秀尚, 大月純子, 立野裕幸, 杉山文博, 小倉淳郎, 小倉淳郎, 小倉淳郎

    日本実験動物学会総会講演要旨集(Web)   70th   2023

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  • 一次精母細胞からのマウス産子作出法の改善と不妊雄マウスへの応用

    越後貫成美, 日野敏昭, 京極博久, 京極博久, 大澤優生, 藤原靖浩, 井上貴美子, 井上貴美子, 田崎秀尚, 田崎秀尚, 大月純子, 大月純子, 国枝哲夫, 水野聖哉, 立野裕幸, 杉山文博, 北島智也, 小倉淳郎, 小倉淳郎, 小倉淳郎

    Journal of Mammalian Ova Research   39 ( 1 )   2022

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  • 顕微授精による一次精母細胞からの産子作出法の改善

    越後貫成美, 日野敏昭, 京極博久, 大澤優生, 水野聖哉, 立野裕幸, 杉山文博, 北島智也, 小倉淳郎, 小倉淳郎, 小倉淳郎

    日本実験動物学会総会講演要旨集(Web)   68th   2021

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  • 卵管内の精子輸送メカニズム

    日野敏昭

    実践 卵管学   87 - 94   2020.12

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    Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher：中外医学社  

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  • マウス一次精母細胞からの産子作出法の改善

    越後貫 成美, 日野 敏昭, 京極 博久, 大澤 優生, 水野 聖哉, 立野 裕幸, 北島 智也, 杉山 文博, 小倉 淳郎

    The Journal of Reproduction and Development   66 ( Suppl. )   j86 - j86   2020.9

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    Language：Japanese   Publisher：(公社)日本繁殖生物学会  

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  • マウス生体における卵管の蠕動と卵管液の流れ 精子の移動や受精との関係

    日野 敏昭

    日本生殖医学会雑誌   64 ( 4 )   360 - 360   2019.10

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    Language：Japanese   Publisher：(一社)日本生殖医学会  

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  • 精子を知る,見る,作る 先体反応の再考察 先体反応はいつどこでおこり,どのような役割を果たすのか?

    日野 敏昭

    日本生殖医学会雑誌   63 ( 3 )   238 - 238   2018.8

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    Language：Japanese   Publisher：(一社)日本生殖医学会  

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  • マウス精子の卵管内における先体反応の惹起と受精までの挙動

    日野 敏昭, 室 悠子, 中野 美和[田村], 岡部 勝, 立野 裕幸, 柳町 隆造

    日本生殖医学会雑誌   61 ( 4 )   423 - 423   2016.10

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    Language：Japanese   Publisher：(一社)日本生殖医学会  

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  • カルシウムイオノフォア処理によるマウス未熟精子の生理的機能獲得

    日野 敏昭

    旭川医科大学研究フォーラム   16   48 - 49   2016.3

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  • 鞭毛および繊毛の運動における軸糸チューブリンポリグリシン化修飾の生物学的意義

    池上 浩司, 日野 敏昭, 中村 健司, 瀬藤 光利

    日本細胞生物学会大会講演要旨集   66回   121 - 121   2014.5

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    Language：Japanese   Publisher：(一社)日本細胞生物学会  

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  • マウス第二極体ゲノムに由来する混倍数性受精卵のゲノム動態とその発生能

    日野 敏昭, 日下部 博一, 立野 裕幸

    Journal of Mammalian Ova Research   31 ( 2 )   S59 - S59   2014.4

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    Language：Japanese   Publisher：(一社)日本卵子学会  

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  • マウス第二極体に由来する混倍数性受精卵のゲノム動態

    日野 敏昭, 日下部 博一, 立野 裕幸

    日本生殖医学会雑誌   58 ( 4 )   378 - 378   2013.10

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  • レトロトランスポゾン由来の真獣類特異的遺伝子Sirh7の胎盤における機能

    石野史敏, 成瀬美衣, 成瀬美衣, 小野竜一, 日野敏昭, 赤塚明, 中村健司, 横山峯介, 横山峯介, 金児(石野)知子

    日本遺伝学会大会プログラム・予稿集   85th   2013

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  • FOR HOW LONG DOES THE SECOND POLAR BODY OF THE MOUSE-FERTILIZED EGG RETAIN GENETIC STABILITY?

    T. Hino, H. Kusakabe, H. Tateno

    FERTILITY AND STERILITY   98 ( 3 )   S163 - S163   2012.9

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  • 哺乳類で新しく獲得された遺伝子Sirh7と哺乳類の胎生進化

    成瀬美衣, 成瀬美衣, 小野竜一, 日野敏昭, 赤塚明, 中村健司, 横山峯介, 石野史敏, 金児(石野)知子

    日本分子生物学会年会プログラム・要旨集(Web)   35th   2012

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  • レトロトランスポゾン由来の遺伝子Sirh7 KOマウス胎盤の解析

    成瀬美衣, 成瀬美衣, 日野敏昭, 赤塚明, 幸田尚, 中村健司, 横山峯介, 小野竜一, 金児(石野)知子, 石野史敏

    日本分子生物学会年会プログラム・要旨集(Web)   34th   2011

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  • 129系マウス卵子における受精障害の原因の解明

    日野 敏昭, 小田 佳奈子, 中村 健司, 立野 裕幸, 豊田 裕, 横山 峯介

    Journal of Mammalian Ova Research   27 ( 2 )   S61 - S61   2010.4

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  • レトロトランスポゾン由来の遺伝子Sirh7の胎盤発生における役割

    成瀬美衣, 日野敏昭, 赤塚明, 幸田尚, 中村健司, 横山峯介, 小野竜一, 金児(石野)知子, 石野史敏

    生化学   83回・33回   ROMBUNNO.3P-0822 - 0822   2010

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  • 129系マウスにおける少ない産仔数の原因の検討

    日野敏昭, 小田佳奈子, 中村健司, 豊田裕, 横山峯介, 横山峯介

    Journal of Mammalian Ova Research   26 ( 2 )   2009

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  • クライオチューブの形状は,凍結保存胚の融解成績に影響を与える

    前田宜俊, 長谷川歩未, 小田加奈子, 日野敏昭, 横山峯介, 横山峯介

    日本実験動物学会総会講演要旨集   56th   2009

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  • 129系マウスにおける低い体外受精率の検討

    日野 敏昭, 小田 佳奈子, 横山 峯介

    日本生殖医学会雑誌   53 ( 4 )   269 - 269   2008.10

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  • レトロトランスポゾン由来の遺伝子Sirh-familyの解析

    成瀬美衣, 成瀬美衣, 小野竜一, 関田洋一, 石井雅之, 中村健司, 日野敏昭, 鈴木(右島)理可, 幸田尚, 横山峯介, 金児(石野)知子, 石野史敏

    生化学   2008

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  • レトロトランスポゾン由来の遺伝子Peg10の胎盤での機能

    小野竜一, 成瀬美衣, 中村健司, 日野敏昭, 鈴木(右島)理可, 高部美穂, 宇佐美貴子, 幸田尚, 小倉淳郎, 横山峯介, 金児(石野)知子

    生化学   2P-1053   2008

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  • マウスMeg1/Grb10領域のインプリンティング発現制御と個体成長への影響

    志浦寛相, 引地貴亮, 中村健司, 日野敏昭, 小田佳奈子, 鈴木(右島)理可, 幸田尚, 金児(石野)知子, 石野史敏

    生化学   2008

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  • マウス卵巣移植における移植卵巣条件の検討

    前田宜俊, 坂井恵美, 日野敏昭, 右島理可, 横山峯介, 横山峯介

    日本実験動物学会総会講演要旨集   55th   2008

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  • Inhibition of hippocampal neurogenesis by X-ray irradiation delays formation of remote memory

    Takashi Kitamura, Yoshito Saitoh, Noriko Takashima, Akiko Murayama, Yosuke Niibori, Toshiaki Hino, Hiroyuki Sugiyama, Kaoru Inokuchi

    NEUROSCIENCE RESEARCH   61   S169 - S169   2008

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  • Peg10, A. Retrotransposon-derived imprinted gene, acts cell autonomousely in mouse spongiotrophoblast development

    R. Ono, M. Naruse, K. Nakamura, T. Hino, R. Suzuki-Migishima, T. Usami, J. C. Cross, T. Kaneko-Ishino, F. Ishino

    PLACENTA   28 ( 8-9 )   A62 - A62   2007.8

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  • マウスMeg1/Grb10のインプリンティング発現制御とその過剰発現の個体への影響

    志浦寛相, 引地貴亮, 中村健司, 日野敏昭, 鈴木(右島)理可, 幸田尚, 金児(石野)知子, 石野史敏

    生化学   2007

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  • レトロトランスポゾンを起源とするPeg11/Rtl1の胎盤での役割

    関田洋一, 我妻広貴, 中村健司, 小野竜一, 鈴木(右島)理可, 日野敏昭, 幸田尚, 小倉淳郎, 横山峯介, 金児(石野)知子, 石野史敏

    生化学   3P-0701   2007

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  • レトロトランスポゾンを利用した哺乳類の胎生進化

    小野竜一, 成瀬美衣, 成瀬美衣, 中村健司, 井上貴美子, 宇佐美貴子, 日野敏昭, 鈴木(右島)理可, 高部美穂, 脇坂紀子, 越後貫成美, 三木洋美, 幸田尚, 小倉淳郎, 横山峯介, CROSS Jay, 金児(石野)知子, 石野史敏

    生化学   2007

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  • Imprinted gene Peg11/Rt11 is derived from retrotransposon and highly expressed in late-fetal placenta.

    Y. Sekita, H. Wagatsurna, R. Ono, T. Kohda, T. Hino, R. Migishima, K. Nakamura, M. Yokoyama, A. Ogura, T. Kaneko-Ishino, F. Ishino

    PLACENTA   27 ( 9-10 )   A37 - A37   2006.9

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  • レトロトランスポゾンを起源とするインプリンティング遺伝子Peg11ノックアウトマウスの解析

    関田洋一, 我妻広貴, 小野竜一, 中村健司, 中原陽子, 鈴木(右島)理可, 高部美穂, 日野敏昭, 幸田尚, 小倉淳郎, 横山峯介, 金児(石野)知子, 石野史敏

    日本分子生物学会年会講演要旨集   28th   204   2005.11

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  • レトロトランスポゾンはほ乳類の胎生進化に寄与したか?

    小野竜一, 成瀬美衣, 中村健司, 井上貴美子, 宇佐見貴子, 日野敏昭, 鈴木(右島)理可, 高部美穂, 脇阪(斎藤, 紀子, 越後貫成美, 三木洋美, 幸田尚, 小倉淳郎, 横山峯介, 金児(石野)知子, 石野史敏

    日本分子生物学会年会講演要旨集   28th   54   2005.11

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  • 様々な糖類によるマウス精子の凍結保存

    日野敏昭, 高部美穂, 右島理可, 横山峯介

    Journal of Mammalian Ova Research   22 ( 2 )   2005

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  • レトロトランスポゾン由来の遺伝子Peg10の解析

    成瀬美衣, 小野竜一, 小野竜一, 中村健司, 日野敏昭, 鈴木(右島)理可, 高部美穂, 井上貴美子, 越後貫成美, 三木洋美, 宇佐美貴子, 脇坂(斎藤)紀子, 脇坂(斎藤)紀子, 脇坂(斎藤)紀子, 脇坂(斎藤)紀子, 幸田尚, 幸田尚, 小倉淳郎, 横山峯介, 横山峯介, 金児(石野)知子, 金児(石野)知子, 石野史敏, 石野史敏

    日本分子生物学会年会講演要旨集   28th   2005

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  • 不安情動行動におけるアクチビンの役割(Activin is involved in anxiety-related brain function)

    上田 洋司, 右島 理可, 茂手木 淑子, 日野 敏昭, 高部 美穂, 喜田 聡, 杉野 弘, 土田 邦博, 横山 峯介, 井ノ口 馨

    神経化学   43 ( 2-3 )   554 - 554   2004.8

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  • Stathminによるプルキンエ細胞樹状突起内微小管の制御と登上繊維・プルキンエ細胞間シナプス除去への関与の可能性(Stathmin regulates dendritic microtubule stability of developing Purkinje cells that might be important for the olivocerebellar synapse elimination)

    大川 宜昭, 右島 理可, 日野 敏昭, 高部 美穂, 横山 峯介, 井ノ口 馨

    神経化学   43 ( 2-3 )   521 - 521   2004.8

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  • 心筋細胞の増殖と分化の制御

    竹内 隆, 中島 久仁子, 小島 瑞代, 高橋 美穂, 日野 敏昭, 高部 美穂, 右島 理可, 横山 峯介

    日本発生生物学会大会講演要旨集   37回   108 - 108   2004.5

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  • レトロトランスポゾン由来のインプリンティング遺伝子Peg10は,ほ乳類の初期発生に必須である

    小野竜一, 中村健司, 日野敏昭, 鈴木(右島)理可, 高部美穂, 井上貴美子, 越後貫成美, 三木洋美, 宇佐美貴子

    日本分子生物学会年会プログラム・講演要旨集   27th   2004

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  • jumonjiはサイクリンD1を介して発生過程における心筋細胞の増殖制御を行う 2重変異体マウスによる解析

    竹内 隆, 高橋 美穂, 中島 久仁子, 小島 瑞代, 大野 忠行, 右島 理可, 日野 敏昭, 高部 美穂, 横山 峯介

    日本発生生物学会大会講演要旨集   36回   87 - 87   2003.6

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  • 発達期小脳の選択的シナプス除去の分子機構:Stathminによるプルキンエ細胞樹状突起内微小管の制御

    大川宜昭, 藤谷和子, 右島理可, 日野敏昭, 高部美穂, 横山峯介, 井ノ口馨

    日本分子生物学会年会プログラム・講演要旨集   26th   2003

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  • ガラス化法による卵巣凍結保存の検討

    右島富士男, 西島正博, 右島理可, 倉持隆司, 日野敏明, 高部美穂, 茂手木淑子, 横山峯介, 東貞宏

    日本不妊学会雑誌   47 ( 4 )   2002

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Presentations

  • マウス卵管内における精子と卵子の輸送機構の検証

    日野 敏昭

    第5回 有性生殖研究会・新学術「全能性」最終公開シンポジウム  2025.3 

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    Event date： 2025.3

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  • 哺乳動物の卵管内における精子輸送機構 Invited

    日野 敏昭

    第16回生殖補助医療胚培養士セミナーおよび倫理講習会  2024.10 

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    Event date： 2024.10 - 2024.11

    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • 様々な糖類によるマウス精子の凍結保存

    日野敏昭, 高部美穂, 右島理可, 横山峯介

    第46回日本哺乳動物卵子学会  2005.5 

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    Venue：青森県八戸市  

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  • マルチカラーFISHによるマウス卵母細胞の核型解析法の開発とその応用

    日野 敏昭, 日下部 博一

    第44回動物生殖工学研究会  2023.12 

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  • マルチカラーFISHを利用したマウス生殖細胞の細胞遺伝学的解析法の開発とその応用

    日野 敏昭

    全能性プログラム：デコーディングからデザインへ 第5回公開シンポジウム  2023.12 

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    Presentation type：Symposium, workshop panel (nominated)  

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  • Development of a method for the identification of each meiotic chromosome using multicolor FISH in mice

    TOSHIAKI HINO

    The International Symposium "Totipotency and Germ Cell Development  2022.11 

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  • マウス卵管内における配偶子・初期胚の輸送機構：生体の卵管を使ってわかったこと

    日野 敏昭

    第8回 生殖若手の会  2022.9 

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  • マウス生体の卵管における卵子輸送の観察 ～卵管液流・繊毛拍動・蠕動運動との関係に着目して～

    日野 敏昭, 柳町 隆造

    第65回 日本卵子学会学術集会  2024.5 

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  • マウス生体の卵管を使った卵管内における配偶子輸送機構の解明 Invited

    日野 敏昭

    第31回 母と子のすこやか基金シリーズセミナー  2024.3 

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  • マウス受精卵における第二極体ゲノムの安定性の検討

    日野敏昭, 日下部博一, 立野裕幸

    財団法人染色体学会 第63回年会  2012.10 

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  • マウス受精卵の第二極体ゲノムの安定性はいつまで保たれるか？

    日野敏昭, 立野裕幸

    第54回 北海道生殖医学会 総会・学術講演会  2012.2 

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  • マウス1細胞期胚の染色体解析における簡易ガラス化法の有用性の検討

    日野敏昭, 日下部博一, 立野裕幸

    第24回動物生殖工学研究会  2010.12 

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    Venue：東京都港区  

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  • 129系マウス卵子における受精障害の原因の解明

    日野敏昭, 小田佳奈子, 中村健司, 立野裕幸, 豊田裕, 横山峯介

    第51回日本哺乳動物卵子学会  2010.5 

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    Venue：新潟県新潟市  

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  • Protective effects of various saccharides on frozen mouse sperm

    日野敏昭, 高部美穂, 右島理可, 上條信一, 横山峯介

    58th American Association for Laboratory Animal Science (AALAS) National Meeting  2007.10 

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    Venue：Charlotte、 NC  

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  • マウス精子に対する各種糖類の凍害保護効果について

    日野敏昭

    第17回動物生殖工学研究会  2005.12 

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    Venue：東京都港区  

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  • 129系マウスにおける少ない産仔数の原因の検討

    日野敏昭, 小田佳奈子, 中村健司, 豊田裕, 横山峯介

    第6回北海道実験動物研究会  2009.7 

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    Venue：北海道帯広市  

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  • 129系マウスにおける少ない産仔数の原因の検討

    日野敏昭, 小田佳奈子, 中村健司, 豊田裕, 横山峯介

    第50回日本哺乳動物卵子学会  2009.5 

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    Venue：東京都千代田区  

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  • 129系マウスにおける低い体外受精率の検討

    日野敏昭, 小田佳奈子, 横山峯介

    第53回日本生殖医学会総会・学術講演会  2008.10 

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    Venue：兵庫県神戸市  

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  • 三菱化学生命科学研究所におけるマウスの体外受精法

    日野敏昭

    第20回動物生殖工学研究会  2007.12 

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    Venue：東京都港区  

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  • マルチカラーFISH法を使ったマウス生殖細胞・初期胚の染色体解析手法の立ち上げ

    日野 敏昭

    【新学術・全能性】領域会議・総括班会議  2021.11 

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  • マウス初期胚の第二極体における半数体ゲノムの安定性と発生運命

    日野 敏昭

    全能性プログラム：デコーディングからデザインへ 第１回公開シンポジウム（キックオフシンポジウム）  2019.11 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • マウス生体における卵管の蠕動と卵管液の流れ：精子の移動や受精との関係

    日野 敏昭, 柳町 隆造

    第64回 日本生殖医学会学術講演会・総会  2019.11 

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  • マウス第二極体に由来する混倍数性受精卵のゲノム動態

    日野敏昭, 日下部博一, 立野裕幸

    第58回 日本生殖医学会 学術講演会・総会  2013.11 

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  • マウス受精卵の第二極体における半数体ゲノムの安定性の検討

    日野敏昭, 日下部博一, 立野裕幸

    第28回 動物生殖工学研究会  2012.12 

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  • How long does the second polar body of the mouse-fertilized egg retain genetic stability?

    Toshiaki Hino, Hirokazu Kusakabe, Hiroyuki Tateno

    アメリカ生殖医学会  2012.10 

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  • Amazingly active peristaltic moments and fluid production of the mouse oviduct: their roles in fluid and sperm transport and fertilization International conference

    Toshiaki Hino, Ryuzo Yanagimachi

    52nd annual conference, Society for the study of the repruduction  2019.7 

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    Language：English   Presentation type：Poster presentation  

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  • 先体反応の再考察：先体反応はいつどこでおこりどのような役割を果たすのか？ Invited

    日野 敏昭

    第63回 日本生殖医学会学術講演会  2018.9 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

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  • マウス精子の卵管内における先体反応の惹起と受精までの挙動

    日野敏昭, 室 悠子, 中野-田村美和, 岡部勝, 立野裕幸, 柳町隆造

    第63回 日本生殖医学会学術講演会  2016.11 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • マウス第二極体ゲノムに由来する混倍数性受精卵のゲノム動態とその発生能

    日野敏昭, 日下部博一, 立野裕幸

    第55回 日本卵子学会  2014.5 

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Awards

  • 第8回生殖若手の会 ベストプレゼンテーション賞

    2022.9   生殖若手の会  

    日野 敏昭

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

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Research Projects

  • 加齢による染色体分配異常の解明にむけたマウス卵母細胞の全染色体同時識別の開発応用

    Grant number：25K09494  2025.4 - 2028.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    日野 敏昭

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    Grant amount：\4,550,000 （ Direct Cost: \3,500,000 、 Indirect Cost：\1,050,000 ）

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  • Study on the mechanism of sperm and embryo transport in oviduct in situ

    Grant number：20K06468  2020.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Toshiaki Hino

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    Grant amount：\4,420,000 （ Direct Cost: \3,400,000 、 Indirect Cost：\1,020,000 ）

    In this study, we examined the transport of zygotes and embryos through the oviduct in situ. We observed that oocytes moved toward the site of fertilization against the fluid flow directed towards the ovary, driven by ciliary movement. Additionally, we found that embryos were propelled towards the uterus due to the peristaltic movement of the oviduct and the resulting fluid flow.

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  • Construction of normal cloned embryos and placentas by improvements of nuclear transfer technology in mice

    Grant number：19H05758  2019.6 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Ogura Atsuo

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    Grant type：Competitive

    Grant amount：\149,240,000 （ Direct Cost: \114,800,000 、 Indirect Cost：\34,440,000 ）

    Somatic cell cloning is a technique to endow the somatic cell genome with totipotency within the recipient ooplasm. We analyzed cloned embryos/placentas at the epigenome level to see how the genome acquires normal/abnormal reprogramming. We found that loss of H3K27me3-dependent imprinting occurred in cloned placentas, leading to placental enlargement at term and abnormal epithelial-mesenchymal transition (EMT) in the early placental tissues. We also found that treatment with a new G9a (histone methyltransferase) inhibitor significantly improved the cloning efficiency.

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  • Study on the mechanism of sperm transport in oviduct

    Grant number：17K08132  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Hino Toshiaki

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4,550,000 （ Direct Cost: \3,500,000 、 Indirect Cost：\1,050,000 ）

    How do spermatozoa in the oviduct migrate from the caudal isthmus to the ampulla where the oocytes await them? In situ observations of the mouse oviduct revealed that spermatozoa migrate to the ampulla not by their own motility but by upward oviduct fluid flow, created by adovarian peristalsis and active fluid secretion. We confirmed that these processes are controlled by ovarian hormones.

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  • 受精能賦活化処理によるマウス未成熟精子の生理的機能変化の促進化

    2014.10 - 2015.9

    旭川医科大学  独創性のある生命科学研究＜個別研究＞ 

    日野 敏昭

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    Authorship：Principal investigator  Grant type：Competitive

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  • 第二極体に由来するマウス混倍数性受精卵の作出と 発生に関する研究

    2012.7 - 2013.3

    公益財団法人 秋山記念生命科学振興財団  研究助成金 

    日野 敏昭

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    Authorship：Principal investigator  Grant type：Competitive

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  • Study on chromosomal stability and developmental potential of mouse second polar bodies

    Grant number：24791678  2012.4 - 2014.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    HINO Toshiaki

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\2,990,000 （ Direct Cost: \2,300,000 、 Indirect Cost：\690,000 ）

    The fusion of a second polar body (PB2) with one of the blastomeres during the cleavage stage has been considered as a causal mechanism underlying diploid-triploid mixoploidy in humans. In this study, we examined the genomic stability of mouse PB2s and whether the PB2s can participate in the generation of mixoploid embryos. Majority of the PB2s remained in the S phase of the cell cycle until 70 h after being emitted without undergoing apoptosis. When the PB2 was fused with one of the 2-cell blastomeres, the chromosomes of the PB2 were incorporated in the mitotic spindle of the blastomere, resulting in the formation of the 2n/3n mixoploid embryo. The 3n blastomeres had the ability to differentiate into the inner cell mass and the trophectderm of blastocysts, and constituted the placenta tissue of the embryo at 11 days after fertilization. The findings suggest that the fusion of the PB2 with a blastomere is one of the causative factors of the formation of the mixoploid embryos.

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  • マウス受精卵の第二極体における半数体ゲノムの 安定性に関する研究

    2010.7 - 2011.6

    旭川医科大学  学術振興講演資金支援事業 

    日野 敏昭

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    Authorship：Principal investigator  Grant type：Competitive

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Teaching Experience

  • 自然科学入門 (生物系）

    Institution：旭川医科大学

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  • 医学チュートリアルⅠ（基礎生物学演習）

    Institution：旭川医科大学

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  • 自然科学実験（生命科学）

    Institution：旭川医科大学

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  • 分子生物学

    Institution：旭川医科大学

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  • 基礎生物学実習

    Institution：旭川医科大学

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  • 科学論文の読み方・書き方

    Institution：旭川医科大学

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  • 遺伝学

    Institution：旭川医科大学

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Social Activities

  • 旭川ウェルビーイング・コンソーシアム「あさひかわオープンカレッジ」

    2023.11

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    １１ 月 ２３日（木）１３：３０～１５：００
    いのちの誕生～卵管の中で卵子と精子が出会うまで～

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  • ハツカネズミの体外受精を見てみよう！

    Role(s)： Lecturer,　Demonstrator

    北海道教育委員会  Dohokuサイエンスフェスティバル  2013.8

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    Type：Science cafe

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  • 平成２５年度 北海道旭川西高等学校SSH事業 「Dohoku サイエンスフェスティバル」（北海道教育委員会主催）

    2013.8

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    本フェスティバルで催されたサイエンス教室に参加し、「命の始まりとは ～ハツカネズミの体外受精を観察してみよう！～」とうタイトルで、ハツカネズミの精子と卵子の「出会い」を、小中高校生の前で約２０分間（×４回）実演した。また、命の始まりについての簡単な説明も行った。

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Academic Activities

  • 第44回動物生殖工学研究会の世話人 International contribution

    2023.12

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    研究会の世話人全般（講演依頼、会場設置、プログラム作製等）

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  • 日本生殖医学会・学術委員会 委員

    2020.12 - 2022.6

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  • 財団法人 染色体学会 第６３回（２０１２年度）年会

    2012.1 - 2012.10

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    大会準備委員

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  • 動物生殖工学研究会・理事

    2001.4

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