Updated on 2025/04/11

写真a

 
YASUDA Satoshi
 
Organization
School of Medicine Medical Course Basic Medicine Biochemistry
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Degree

  • Doctor of Philosophy ( 2014.3   Osaka University )

Research Areas

  • Life Science / Functional biochemistry

  • Life Science / Biophysics

Research History

  • Asahikawa Medical College   Assistant Professor

    2016.4

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Papers

  • Electrostatic interactions between single arginine and phospholipids modulate physiological properties of sarcoplasmic reticulum Ca2+-ATPase Reviewed

    Kazuo Yamasaki, Takashi Daiho, Satoshi Yasuda, Stefania Danko, Jun-ichi Kawabe, Hiroshi Suzuki

    Scientific Reports   12 ( 1 )   2022.7

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    Arg324 of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase forms electrostatic interactions with the phosphate moiety of phospholipids in most reaction states, and a hydrogen bond with Tyr122 in other states. Using site-directed mutagenesis, we explored the functional roles of Arg324 interactions, especially those with lipids, which at first glance might seem too weak to modulate the function of such a large membrane protein. The hydrogen bond forms transiently and facilitates Ca<sup>2+</sup> binding from the cytoplasmic side. The contributions of the electrostatic interactions to the reaction steps were quantified using a rate vs activity coefficient plot. We found that the interaction between Arg324 and lipids decreases the affinity for luminal Ca<sup>2+</sup>. The transformation rate of the phosphoenzyme intermediate is facilitated by the electrostatic interactions, and the function of these interactions depends not only on the type but also on the composition of the phospholipids. The properties observed in microsomes could not be reproduced with any single phospholipid, but with a mixture of phospholipids that mimics the native membrane. These results suggest the importance of swapping of the lipid partners of different headgroups in the reaction step. This study shows that Arg324 plays a role in the reaction cycle via complex intra-protein and protein-lipid interactions.

    DOI: 10.1038/s41598-022-16091-9

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    Other Link: https://www.nature.com/articles/s41598-022-16091-9

  • Nanodisc-based kinetic assays reveal distinct effects of phospholipid headgroups on the phosphoenzyme transition of sarcoplasmic reticulum Ca2+-ATPase Reviewed

    Kazuo Yamasaki, Takashi Daiho, Stefania Danko, Satoshi Yasuda, Hiroshi Suzuki

    Journal of Biological Chemistry   292 ( 49 )   20218 - 20227   2017.12

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1074/jbc.m117.816702

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  • Nucleotide-dependent displacement and dynamics of the α-1 helix in kinesin revealed by site-directed spin labeling EPR Reviewed

    Satoshi Yasuda, Takanori Yanagi, Masafumi D. Yamada, Shoji Ueki, Shinsaku Maruta, Akio Inoue, Toshiaki Arata

    Biochemical and Biophysical Research Communications   443 ( 3 )   911 - 916   2014.1

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bbrc.2013.12.063

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  • Spatial arrangement of rhodopsin in retinal rod outer segment membranes studied by spin-labeling and pulsed electron double resonance Reviewed

    Satoshi Yasuda, Hideyuki Hara, Fumio Tokunaga, Toshiaki Arata

    Biochemical and Biophysical Research Communications   425 ( 2 )   134 - 137   2012.8

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bbrc.2012.07.040

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  • Conformational dynamics of loops L11 and L12 of kinesin as revealed by spin-labeling EPR Reviewed

    Masafumi D. Yamada, Shinsaku Maruta, Satoshi Yasuda, Kazunori Kondo, Hidekatsu Maeda, Toshiaki Arata

    Biochemical and Biophysical Research Communications   364 ( 3 )   620 - 626   2007.12

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bbrc.2007.10.043

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  • Dynamic structures of motor proteins myosin and kinesin, and switch protein troponin as detected by SDSL-ESR

    Toshiaki Arata, Motoyoshi Nakamura, Shoji Ueki, Kazunori Sugata, Tomoki Aihara, Keisuke Ueda, Satoshi Yasuda, Ryouhei Narumi, Hiroko Kusuhara, Yukio Yamamoto

    Microscopy   54 ( suppl_1 )   i47 - i51   2005.9

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    Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    DOI: 10.1093/jmicro/54.suppl_1.i47

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MISC

  • 筋小胞体Ca<sup>2+</sup>-ATPaseのリン酸化中間体形成過程に対する脂質の違いによる影響

    山崎和生, 安田哲, 大保貴嗣, 川辺淳一

    日本生化学会大会(Web)   96th   2023

  • Effects of lipid environments on EP formation steps of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase.

    山崎和生, 安田哲, 大保貴嗣, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   49th   2023

  • Functional roles of interaction of M2/M6 helix in energy coupling of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase

    大保貴嗣, 山崎和生, 安田哲, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   48th   2022

  • 筋小胞体CaポンプのヘリックスM2/M6のCa輸送における役割

    大保貴嗣, 山崎和生, 安田哲, 川辺淳一

    日本生化学会大会(Web)   95th   2022

  • 混合脂質環境における筋小胞体Ca<sup>2+</sup>-ATPaseの特性の変化について

    山崎和生, 安田哲, 大保貴嗣, 川辺淳一

    日本生化学会大会(Web)   95th   2022

  • Effects of mixed lipid environment on the enzymatic properties of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase

    山崎和生, 安田哲, 大保貴嗣, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   48th   2022

  • 筋小胞体カルシウムポンプの多機能側鎖Arg<sup>324</sup>と多種のリン脂質のアンサンブル

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 川辺淳一, 鈴木裕

    日本生化学会大会(Web)   94th   2021

  • Diversity of lipid-protein interaction mediated by multi-functional residue Arg<sup>324</sup> of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase.

    山崎和生, 大保貴嗣, 安田哲, DANKO Stefania, 川辺淳一, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   47th (CD-ROM)   2021

  • 筋小胞体カルシウムポンプの結晶構造中に見られたArg<sup>324</sup>とリン脂質間のリンクが果たす役割について

    山崎和生, 大保貴嗣, 安田哲, DANKO Stefania, 川辺淳一, 鈴木裕

    日本生化学会大会(Web)   93rd   2020

  • ヒトフリッパーゼATP8A1とCaポンプの反応機構の比較

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, 申惠媛, STEFANIA Danko, 川辺淳一, 鈴木裕

    日本生化学会大会(Web)   93rd   2020

  • Human flippase ATP8A1 reaction mechanism.

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, SHIN Hye-Won, DANKO Stefania, 川辺淳一, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   46th   2020

  • 筋小胞体Ca<sup>2+</sup>ポンプのM2ヘリックス:膜貫通部分のエネルギー共役における役割

    大保貴嗣, 山崎和生, 安田哲, 川辺淳一

    日本生化学会大会(Web)   93rd   2020

  • Functional roles of protein-lipid interaction between Arg<sup>324</sup> and phospholipids observed in crystal structures of sarcoplasmic reticulum Ca<sup>2+</sup>-pump

    山崎和生, 大保貴嗣, 安田哲, DANKO Stefania, 川辺淳一, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   46th   2020

  • Functional roles of M2 membrane-spanning helix in energy coupling of Sarcoplasmic Reticulum Ca<sup>2+</sup>-ATPase

    大保貴嗣, 山崎和生, 安田哲, 川辺淳一

    日本生体エネルギー研究会討論会講演要旨集   46th   2020

  • 筋小胞体Ca2+ポンプの第2膜貫通ヘリックス:膜貫通部分のエネルギー共役における役割

    大保貴嗣, 山崎和生, 安田哲, STEFANIA Danko

    日本生化学会大会(Web)   92nd   2019

  • 筋小胞体カルシウムポンプのTyr<sup>122</sup>クラスター変異体で観測された異常に遅いEP分解

    山崎和生, 市村祐人, 山崎誠一郎, 大保貴嗣, 安田哲, STEFANIA Danko, 鈴木裕

    日本生化学会大会(Web)   92nd   2019

  • Effect of phospholipids on human flippase ATP8A1 reaction mechanism.

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, SHIN Hye-Won, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   45th   2019

  • Second Transmembrane Helix (M2) of Sarcoplasmic Reticulum Ca<sup>2+</sup>-ATPase; Functional roles of M2 membrane-spanning region in energy coupling

    大保貴嗣, 山崎和生, DANKO Stefania, 安田哲

    日本生体エネルギー研究会討論会講演要旨集   45th   2019

  • Analysis of Tyr<sup>122</sup>-hydorophobic cluster mutants of sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase with equations of classical physical chemistry-Exploring the method to quantify the contribution of hydrophobic-interactions in enzymatic reaction-

    山崎和生, 山崎誠一郎, 市村佑人, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   45th   2019

  • ヒトフリッパーゼATP8A1の反応機構における脂質の影響

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, 申惠媛, Danko Stefania, 鈴木裕

    日本生化学会大会(Web)   92nd   2019

  • フリッパーゼATP8A1の反応と安定性に対する界面活性剤および脂質の影響

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, 申惠媛, DANKO Stefania, 鈴木裕

    日本生化学会大会(Web)   91st   2018

  • ヒトフリッパーゼATP8A1の反応機構解析

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, SHIN Hye-Won, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   44th   2018

  • 筋小胞体Ca<sup>2+</sup>-ATPaseの脱共役モードについて

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生化学会大会(Web)   91st   2018

  • 筋小胞体カルシウムポンプの非リン酸化中間体におけるCa2+結合に及ぼす界面活性剤の影響

    大保貴嗣, 山崎和生, DANKO Stefania, 安田哲, 鈴木裕

    日本生化学会大会(Web)   91st   2018

  • 筋小胞体カルシウムポンプのCa<sup>2+</sup>結合に及ぼす界面活性剤の効果

    大保貴嗣, DANKO Stefania, 山崎和生, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   44th   2018

  • 筋小胞体Ca<sup>2+</sup>-ATPaseの脱共役モードについて

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   44th   2018

  • ナノディスクに組み込んだ筋小胞体Ca<sup>2+</sup>-ATPaseの活性に対する膜表面電荷の影響

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生化学会大会(Web)   90th   2017

  • 筋小胞体カルシウムポンプ活性に対する脂質ヘッドグループ及び膜表面電荷の影響の解析

    山崎和生, 大保貴嗣, DANKO Stefania, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   43rd   2017

  • 筋小胞体カルシウムポンプの反応に及ぼす界面活性剤の効果

    大保貴嗣, 山崎和生, DANKO Stefania, 安田哲, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   43rd   2017

  • フリッパーゼの反応機構解析

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, SHIN Hye-Won, DANKO Stefania, 鈴木裕

    日本生体エネルギー研究会討論会講演要旨集   43rd   2017

  • フリッパーゼの生化学的反応機構の解析

    安田哲, 山崎和生, 大保貴嗣, 高津宏之, 申惠媛, DANKO Stefania, 鈴木裕

    日本生化学会大会(Web)   90th   2017

  • 筋小胞体カルシウムポンプの活性に対する界面活性剤の影響

    大保貴嗣, 山崎和生, DANKO Stefania, 安田哲, 鈴木裕

    日本生化学会大会(Web)   90th   2017

  • Structural dynamics in metal ion coordination of copper pump P-type ATPase as studied by EPR spectroscopy: Effect of ATP

    YASUDA Satoshi, YASUDA Satoshi, KUWABARA Naoyuki, UEKI Shoji, ARATA Toshiaki, ARATA Toshiaki

    生物物理(Web)   56 ( Supplement 1-2 )   2016

  • 電子スピン共鳴法を用いた銅輸送P-type ATPaseの輸送サイトの研究

    安田哲, 安田哲, 植田恭広, 堀本拓也, 大門大朗, 植木正二, 桑原直之, 荒田敏昭

    日本生化学会大会(Web)   89th   2016

  • ESRによる銅輸送P-type ATPase(CopB)の輸送サイトの研究

    安田哲, 植田恭広, 堀本拓也, 大門大朗, 植木正二, 桑原直之, 荒田敏昭

    日本生体エネルギー研究会討論会講演要旨集   41st   2015

  • ESRによる銅Cu<sup>2+</sup>(II)輸送P1B-type ATPaseの常磁性金属イオン結合構造の解析

    安田哲, 植田恭広, 堀本拓也, 植木正二, 桑原直之, 荒田敏昭

    生体分子科学討論会講演要旨集   42nd   2015

  • ATP加水分解中の銅輸送P型ATPaseにおけるイオン結合構造のESR解析

    YASUDA Satoshi, DAIMON Hiroaki, UEDA Yasuhiro, HORIMOTO Takuya, UEKI Shoji, KUWABARA Naoyuki, ARATA Toshiaki, ARATA Toshiaki

    日本生化学会大会(Web)   88th   2015

  • ESR Spectroscopy on Metal binding Sites in Cu<sup>2+</sup> -Transporting Protein during ATP Hydrolysis

    YASUDA Satoshi, HORIMOTO Takuya, DAIMON Hiroaki, UEDA Yasuhiro, KUWABARA Naoyuki, ARATA Toshiaki

    生物物理(Web)   55 ( Supplement 1-2 )   2015

  • ESRを用いた銅輸送タンパク質の輸送サイトの研究

    安田哲, 植田恭広, 堀本拓也, 大門大朗, 植木正二, 桑原直之, 荒田敏昭

    電子スピンサイエンス学会年会講演要旨集   54th   2015

  • ESRによる銅Cu<sup>2+</sup>(II)輸送P1B-type ATPaseの常磁性金属イオン結合構造の解析

    安田哲, 植田恭広, 堀本拓也, 植木正二, 桑原直之, 荒田敏昭

    日本生体エネルギー研究会討論会講演要旨集   40th   2014

  • ESR Spectroscopy on Metal binding Sites in Cu<sup>2+</sup>-Transporting ATPase Protein

    YASUDA Satoshi, HORIMOTO Takuya, DAIMON Hiroaki, UEDA Yasuhiro, KUWABARA Naoyuki, ARATA Toshiaki

    電子スピンサイエンス学会年会講演要旨集   53rd   2014

  • 銅Cu(II)輸送P1B-type ATPaseの金属イオン結合構造のESR解析

    安田哲, 堀本拓也, 大門大朗, 植田恭広, 植木正二, 桑原直之, 荒田敏昭, 荒田敏昭

    日本生化学会大会(Web)   87th   2014

  • 微小管に結合したキネシンにおけるα-1ヘリックスのヌクレオチド依存的動的構造:スピンラベルESR距離測定による検出

    安田哲, 楊貴成, 山田正文, 丸田晋策, 荒田敏昭

    日本生化学会大会(Web)   86th   2013

  • キネシンにおけるα-1ヘリックスのヌクレオチド依存的変位:スピンラベルESR距離測定による検出

    安田哲, 楊貴成, 山田正文, 丸田晋策, 荒田敏昭

    電子スピンサイエンス学会年会講演要旨集   52nd   2013

  • Nucleotide-dependent Displacement and Dynamics of α-1 Helix in Motor Protein Kinesin As Revealed by Site Directed Spin Labeling ESR

    YASUDA Satoshi, YANAGI Takanori, YAMADA Masafumi, MARUTA Shinsaku, ARATA Toshiaki

    生物物理   53 ( Supplement 1-2 (Web) )   2013

  • 微小管に結合中のキネシンにおいてATP結合とネックリンカーのドッキングとを連動させるα-1ヘリックスの変位:ESRによる検出

    安田哲, 楊貴成, 高井進二, 山田正文, 丸田晋策, 植木正二, 荒田敏昭

    日本生体エネルギー研究会討論会講演要旨集   39th   2013

  • Structural dynamics of necklinker docking, interlinker strain and nucleotide-to-linker transmission in kinesin probed by spin-label ESR

    YASUDA Satoshi, TAKAI Shinji, YAMADA Masafumi D., MARUTA Shinsaku, ARATA Toshiaki

    生物物理   52 ( Supplement 1 )   2012

  • スピンラベルとパルス電子二重共鳴による網膜桿体外節膜内のロドプシン空間分布の研究

    安田哲, 原英之, 徳永史生, 荒田敏昭

    電子スピンサイエンス学会年会講演要旨集   51st   2012

  • Conformational Change of alfa-1 helix of Kinesin

    Satoshi Yasuda, Masafumi Yamada, Shinsaku Maruta, Toshiaki Arata

    Biophysical Journal   100 ( 3 )   146a - 146a   2011.2

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    Publisher:Elsevier BV  

    DOI: 10.1016/j.bpj.2010.12.1004

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  • ESRを用いたキネシンのアルファ1ヘリックスの構造変化の解析

    安田哲, 山田正文, 丸田晋策, 荒田敏昭

    生化学   2011

  • 1M1336 Conformational change of alfa-1 helix of kinesin detected by electron paramagnetic resonance (EPR)(Molecular motor 2,The 49th Annual Meeting of the Biophysical Society of Japan)

    Satoshi Yasuda, Masafumi Yamada, Shinsaku Maruta, Toshiaki Arata

    Seibutsu Butsuri   51 ( supplement )   S63 - S63   2011

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    Language:English   Publisher:Biophysical Society of Japan  

    DOI: 10.2142/biophys.51.s63_4

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  • Conformational change of alfa-1 helix of kinesin detected by electron paramagnetic resonance (EPR)

    YASUDA Satoshi, YAMADA Masafumi, MARUTA Shinsaku, ARATA Toshiaki

    生物物理   51 ( Supplement 1 )   2011

  • スピンラベルESRによる生物分子機械タンパク質の作動原理の解明

    荒田敏昭, 石井健太郎, 石井健太郎, 高井進二, 安田哲, 山本晃衣, ZHAO Chenchao, 宗宮孝安, 植田啓介, 植田啓介

    電子スピンサイエンス学会年会講演要旨集   50th   2011

  • Conformational change of α1 helix of kinesin

    YASUDA Satoshi, YAMADA Masafumi D., YAMADA Masafumi D., MARUTA Shinsaku, ARATA Toshiaki

    生物物理   50 ( Supplement 2 )   2010

  • スピンラベルESRによるキネシンのα-1ヘリックスの構造変化の検出

    安田哲, 山田正文, 丸田晋策, 荒田敏昭

    電子スピンサイエンス学会年会講演要旨集   49th   2010

  • Conformational change of -1 helix of kinesin

    YASUDA Satoshi, YAMADA Masafumi, MARUTA Shinsaku, ARATA Toshiaki

    生化学   2010

  • Analysis of Conformational Change of Novel Rice Kinesin K16 Using Small Angle X-ray Solution Scattering and EPR

    Yuko Kubo, Satoshi Yasuda, Yasunobu Sugimoto, Masafumi D. Yamada, Toshiaki Arata, Katsuzo Wakabayashi, Shinsaku Maruta

    Biophysical Journal   96 ( 3 )   138a - 138a   2009.2

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    Publisher:Elsevier BV  

    DOI: 10.1016/j.bpj.2008.12.631

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  • Detection of structural movement in nucleotide-binding pocket of kinesin using by site-directed spin labeling ESR(electron spin resonance)

    YASUDA Satoshi, YAMADA Masafumi, MARUTA Shinsaku, ARATA Toshiaki

    生化学   2008

  • 3P-149 Study of the dynamic structure of kinesin by site-directed spin labeling ESR (electron spin resonance)(The 46th Annual Meeting of the Biophysical Society of Japan)

    Satoshi Yasuda, Masafumi D. Yamada, Shinsaku Maruta, Toshiaki Arata

    Seibutsu Butsuri   48 ( supplement )   S150 - S150   2008

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    Publisher:Biophysical Society of Japan  

    DOI: 10.2142/biophys.48.s150_5

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  • Detection of substrate induced conformational changes of Na<sup>+</sup>/H<sup>+</sup> antiporter(NhaA) by SDSL-ESR

    KUWABARA Naoyuki, KUWABARA Naoyuki, YAMAKAMI Suguru, YASUDA Satoshi, UEKI Shoji, ARATA Toshiaki, KANAZAWA Hiroshi

    生化学   2007

  • 1P112 Detection of substrate induced conformational changes of Na^+/H^+ antiporter (NhaA) by SDSL-ESR(Membrane proteins,Poster Presentations)

    Naoyuki Kuwabara, Suguru Yamakami, Satoshi Yasuda, Shoji Ueki, Toshiaki Arata, Hiroshi Kanazawa

    Seibutsu Butsuri   47 ( supplement )   S51 - S51   2007

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    Publisher:Biophysical Society of Japan  

    DOI: 10.2142/biophys.47.s51_3

    J-GLOBAL

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Presentations

  • Uncoupling mechanism of the sarcoplasmic reticulum calcium pump revealed by temperature dependence

    2024.12 

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    Event date: 2024.12

    Presentation type:Oral presentation (general)  

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  • Uncoupling mechanism of the sarcoplasmic reticulum calcium pump revealed by temperature dependence

    Kazuo Yamasaki, Satoshi Yasuda, Jun-ichi Kawabe

    2024.11 

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    Event date: 2024.11

    Language:Japanese   Presentation type:Poster presentation  

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  • 筋小胞体Ca2+-ATPaseのリン酸化中間体形成過程に対する脂質の違いによる影響

    〇山崎 和生, 安田 哲, 大保 貴嗣, 川辺 淳一

    日本生体エネルギー研究会 第49回討論会  2023.12 

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    Event date: 2023.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 筋小胞体Ca2+-ATPaseのリン酸化中間体形成過程に対する脂質の違いによる影響

    〇山崎 和生, 安田 哲, 大保 貴嗣, 川辺 淳一

    第96回日本生化学会大会  2023.10 

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    Event date: 2023.10 - 2023.11

    Language:Japanese   Presentation type:Poster presentation  

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  • 混合脂質環境における筋⼩胞体Ca2+-ATPaseの特性の変化について

    〇山崎 和生, 安田 哲, 大保 貴嗣, 川辺 淳一

    日本生体エネルギー研究会第48回討論会  2022.12 

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    Event date: 2022.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 混合脂質環境における筋小胞体Ca2+-ATPaseの特性の変化について

    〇山崎 和生, 安田 哲, 大保 貴嗣, 川辺 淳一

    第95回 日本生化学会大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Poster presentation  

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  • 筋小胞体カルシウムポンプの多機能性残基 Arg324 と脂質との相互作用の多様性について

    〇山崎和生, 大保貴嗣, Danko Stefania, 安田 哲, 川辺 淳一, 鈴木裕

    日本生体エネルギー研究会第47回討論会  2021.12 

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    Event date: 2021.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Reaction mechanism of human flippase ATP8A1

    2020.12 

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    Event date: 2020.12

    Presentation type:Oral presentation (general)  

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  • Comparison of reaction mechanisms between human flippase ATP8A1 and Ca pump

    2020.9 

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    Event date: 2020.9

    Presentation type:Poster presentation  

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  • Effect of phospholipids on each reaction step of human flippase ATP8A1

    2019.12 

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    Event date: 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Effect of lipids on the reaction mechanism of human flippase ATP8A1

    2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Poster presentation  

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  • ヒトフリッパーゼATP8A1の反応機構

    〇安田 哲, 山崎 和生, 大保 貴嗣, 高津 宏之, 申 惠媛, Stefania Danko, 鈴木 裕

    日本生体エネルギー研究会 第44回討論会 

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    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 筋小胞体Ca2+-ATPaseの脱共役モードについて

    〇山崎和生, 大保貴嗣, Danko Stefania, 安田 哲, 鈴木裕

    日本生体エネルギー研究会 第44回討論会  2018.12 

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    Event date: 2018.12

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  • 筋小胞体カルシウムポンプのCa2+結合に及ぼす界面活性剤の効果

    〇大保 貴嗣, 山崎 和生, Danko Stefania, 安田 哲, 鈴木 裕

    日本生体エネルギー研究会 第44回討論会  2018.12 

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    Event date: 2018.12

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  • Membrane Perturbation of ADP-insensitive Phosphoenzyme of Ca2+-ATPase Modifies Gathering of Transmembrane Helix M2 with Cytoplasmic Domains and Luminal Gating

    S. Danko, K. Yamasaki, T. Daiho, S. Yasuda, H. Suzuki

    The 15th Inthernational Conference on Na,K-ATPase and Related Transport ATPases 

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    Event date: 2017.9

    Language:English   Presentation type:Poster presentation  

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  • Effects of phospholipid's head groups on the properties of sarcoplasmic reticulum Ca2+-ATPase embedded in nanodisc

    K. Yamasaki, T. Daiho, S. Danko, S. Yasuda, H. Suzuki

    The 15th Inthernational Conference on Na,K-ATPase and Related Transport ATPases 

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    Event date: 2017.9

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  • Electron spin resonance study on transport site in Cu2+-Transporting Protein

    2017.9 

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    Event date: 2017.9

    Language:English   Presentation type:Poster presentation  

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  • Nucleotide-dependent Displacement and Dynamics of Alpha-1 Helix in Motor Protein Kinesin As Revealed by Site Directed Spin Labeling ESR

    The 1st Awaji International Workshop on Electron Spin Science & Technology: Biological and Materials Science Oriented Applications  2013.6 

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    Event date: 2013.6

    Language:English   Presentation type:Poster presentation  

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  • Conformational change of α-1 helix of kinesin detected by electron paramagnetic resonance (EPR)

    The 49th Annual Meeting of the Biophysical Society of Japan  2011.9 

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    Event date: 2011.9

    Language:English   Presentation type:Oral presentation (general)  

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  • Conformational change of α-1 helix of kinesin detected by site-directed spin labeling ESR (electron spin resonance)

    The Biophysical Society's 55th Annual Meeting  2011.3 

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    Event date: 2011.3

    Language:English   Presentation type:Poster presentation  

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  • ESR Spectroscopy on Metal Binding Sites in Cu2+-Transporting Protein during ATP Hydrolysis

    The 3rd Awaji International Workshop on Electron Spin Science & Technology: Biological and Materials Science Oriented Applications  2015.6 

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Research Projects

  • Effect of lipids on the reaction mechanism of human ATP8A1

    2019.9 - 2020.3

    Akiyama Life Science Foundation 

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    Authorship:Principal investigator 

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  • P-type pump ; Molecular basis of energy coupling and carcinogenesis caused by defects

    Grant number:15H04346  2017.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Suzuki Hiroshi, MASAIKE Tomoko, MINOWA Osamu, DANKO Stefania

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    Authorship:Coinvestigator(s) 

    Grant amount:\16,250,000 ( Direct Cost: \12,500,000 、 Indirect Cost:\3,750,000 )

    Gating mechanism of sarco(endo)plasmic reticulum Ca-pump SERCA1a and involvement of lipid on membrane in the pump function were studied, thereby understanding of energy coupling mechanism in the active Ca2+ transport were further deepened. By the use of the Ca2+-pump, the methods for a single molecule dynamics were developed, and the structural changes of the pump were analyzed for understanding the structural mechanism. For the flippase, another P-type ATPase, studies were performed to identify its specific substrates, and reveal the mechanism. Fur types of mutations of endoplasmic Ca2+-pump SERCA2b that develop squamous cell carcinoma in mouse model were identified, and the pathological consequences and defects in the pump protein were revealed.

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Teaching Experience

  • Biochemistry

    2023.4 Institution:Asahikawa Medical College

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  • Biochemistry

    2016.4 Institution:Asahikawa Medical College

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Social Activities

  • わくわくサイエンス

    Role(s): Organizing member, Demonstrator

    2025.2

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  • エコチルサイエンス

    2024.9

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  • わくわくサイエンス in 科学館

    Role(s): Organizing member, Demonstrator

    2024.6

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  • わくわくサイエンス

    Role(s): Organizing member, Demonstrator

    2024.2

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    Type:Other

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  • わくわくサイエンス

    Role(s): Organizing member, Demonstrator

    2023.6

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    Type:Other

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  • わくわくサイエンス

    Role(s): Organizing member, Demonstrator

    2023.2

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    Type:Other

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  • わくわくサイエンス

    Role(s): Organizing member, Demonstrator

    2022.6

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    Type:Other

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  • 旭川西高校SSH事業(講座名【医学研究アラカルト】)

    Role(s): Lecturer

    2021.11

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    Type:Visiting lecture

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  • 旭川西高校SSH事業(講座名【医学研究アラカルト】)

    Role(s): Lecturer

    2020.12

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    Type:Visiting lecture

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  • 旭川西高校SSH事業(講座名【医学研究アラカルト】)

    Role(s): Lecturer

    2019.11

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    Type:Visiting lecture

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  • わくわくサイエンス

    Role(s): Demonstrator

    2019.2

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  • わくわくサイエンス

    Role(s): Demonstrator

    2018.2

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